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The beads were designed to have a Young's modulus of\napproximately 4.3 \u00b1 1.5 kPa. These measurements were used to illustrate the\ncorrection of systematic errors for the computation of the Young's modulus in\nRT-DC (figure 10 in [1]). The reservoir measurement was added for the sake of\ncompleteness. The files were converted from the .tdms file format to the .rtdc\nfile format using DCKit 0.10.0 [2] and dclab 0.27.8 [3].  \n  \nBefore analysis, the data should be filtered for porosity (1.0 < area_ratio <\n1.1) and deformation (0 < deform < 0.035). This is necessary, because events\nwere not always detected correctly (incorrect contour data) due to lack of\nimaging contrast.  \n  \n  \n **References:**  \n[1] Christoph Herold. Mapping of Deformation to Apparent Young\u2019s Modulus in\nReal-Time Deformability Cytometry. 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"metadata_modified": "2025-07-05T02:02:33.196443", "mimetype": "text/plain", "mimetype_inner": null, "name": "README.txt", "package_id": "c7a98a04-4e0a-98a7-fb0b-eca379d1f219", "position": 4, "resource_type": null, "s3_available": true, "s3_url": "https://objectstore.hpccloud.mpcdf.mpg.de/circle-5a7a053d-55fb-4f99-960c-f478d0bd418f/resource/659/483/d5-e04d-6fb9-e6c4-a33e482fc411", "sha256": "ce74ac179b26576e0252485a26a584457a0026331a061e85360cec5583660796", "size": 1640, "state": "active", "url": "https://dcor.mpl.mpg.de/dataset/c7a98a04-4e0a-98a7-fb0b-eca379d1f219/resource/659483d5-e04d-6fb9-e6c4-a33e482fc411/download/readme.txt", "url_type": "upload"}], "tags": [], "groups": [], "relationships_as_subject": [], "relationships_as_object": []}, {"authors": "Miguel Xavier, Philipp Rosendahl, Paul M\u00fcller, Maik Herbig, Jochen Guck", "creator_user_id": "d76d937b-3601-4559-92da-585a47c257f5", "doi": "10.6084/m9.figshare.11662773.v2", "id": "f0b5f678-e4f0-c623-d2e4-c0f28d4c8a7a", "isopen": true, "license_id": "CC0-1.0", "license_title": "Creative Commons Public Domain Dedication", "license_url": "https://creativecommons.org/publicdomain/zero/1.0/", "metadata_created": "2020-09-16T16:02:11.704039", "metadata_modified": "2025-07-05T02:02:32.887605", "name": "figshare-11662773-v2", "notes": "**Real-time deformability data of primary human skeletal stem cells and the\nhuman osteosarcoma cell line MG-63**  \n  \nThe MG-63 cell line is used as a model system for human skeletal stem cells\n(SSC). See references [1] and [2] for more information. The file names follow\nthe pattern _\"[sample]_[flow rate/reservoir]_[repetition]_[date].rtdc\"_ , i.e.\nthe file \"SSC_32uls_rep1_20150611.rtdc\" contains human skeletal stem cell data\nmeasured at a flow rate of 32\u00b5l/s - it is the first of two repetitions.  \n  \n  \nThis is a subset of the data used by Xavier et al. [1] to quantify the\nstiffness of MG-63 and SSC cells. Herbig et al. [2] used the 16\u00b5l/s data in\nsection IIID to illustrate linear mixed model analysis.  \n  \n  \n **References  \n** [1] M. Xavier et al., Mechanical phenotyping of primary human skeletal stem\ncells in heterogeneous populations by real-time deformability cytometry,\nIntegrative Biology, 2016, doi:10.1039/C5IB00304K  \n  \n[2] M. Herbig et al., Statistics for real-time deformability cytometry:\nClustering, dimensionality reduction, and significance testing,\nBiomicrofluidics, 2018. doi:10.1063/1.5027197  \n  \n\n", "num_resources": 17, "num_tags": 0, "organization": {"id": "5a7a053d-55fb-4f99-960c-f478d0bd418f", "name": "figshare-import", "title": "Figshare mirror", "type": "organization", "description": "This lab contains selected data imported from figshare. If you would like your dataset to appear here, please send the figshare DOI to Paul M\u00fcller.", "image_url": "", "created": "2020-09-16T15:58:45.009115", "is_organization": true, "approval_status": "approved", "state": "active"}, "owner_org": "5a7a053d-55fb-4f99-960c-f478d0bd418f", "private": false, "references": "doi:10.1039/C5IB00304K, doi:10.1063/1.5027197", "state": "active", "title": "Real-time deformability cytometry data of primary human skeletal stem cells and the human osteosarcoma cell line MG-63", "type": "dataset", "resources": [{"cache_last_updated": null, "cache_url": null, "created": "2020-09-16T16:02:12.719109", "dc:experiment:date": "2015-06-11", "dc:experiment:event count": 1793, "dc:experiment:run index": 4, "dc:experiment:sample": "SSC reservoir rep2", "dc:experiment:time": "16:18:51", "dc:imaging:flash device": "LED (undefined)", "dc:imaging:flash duration": 2.0, "dc:imaging:frame rate": 500.0, "dc:imaging:pixel size": 0.34, "dc:imaging:roi position x": 504, 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