Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f53b81b292a1fc76e4f7e17d21226c97",
"name": "M1_20170515_1521_dsRNA_A8_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:10:09"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8372 (max 8373) in '8372'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.3954
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.51303e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 30 mean_circ: 0.941044
Ran for 2050 frames Cell Events: 108 mean_circ: 0.940207
Ran for 2550 frames Cell Events: 124 mean_circ: 0.939827
Ran for 4050 frames Cell Events: 200 mean_circ: 0.940224
Ran for 4550 frames Cell Events: 221 mean_circ: 0.940156
Ran for 5550 frames Cell Events: 281 mean_circ: 0.9408
Ran for 6050 frames Cell Events: 299 mean_circ: 0.939841
Ran for 8050 frames Cell Events: 371 mean_circ: 0.936543
Ran for 9550 frames Cell Events: 445 mean_circ: 0.936557
Ran for 11050 frames Cell Events: 533 mean_circ: 0.935803
Ran for 11550 frames Cell Events: 558 mean_circ: 0.935423
Ran for 12050 frames Cell Events: 586 mean_circ: 0.935293
Ran for 13050 frames Cell Events: 632 mean_circ: 0.935704
Ran for 13550 frames Cell Events: 651 mean_circ: 0.93513
Ran for 14050 frames Cell Events: 670 mean_circ: 0.935544
Ran for 15050 frames Cell Events: 721 mean_circ: 0.935641
Ran for 15550 frames Cell Events: 740 mean_circ: 0.935924
Ran for 16050 frames Cell Events: 766 mean_circ: 0.93589
Ran for 16550 frames Cell Events: 796 mean_circ: 0.935706
Ran for 17050 frames Cell Events: 822 mean_circ: 0.93604
Ran for 18050 frames Cell Events: 876 mean_circ: 0.935535
Ran for 18550 frames Cell Events: 898 mean_circ: 0.935535
Ran for 19550 frames Cell Events: 947 mean_circ: 0.936
Ran for 20550 frames Cell Events: 997 mean_circ: 0.935328
Ran for 21050 frames Cell Events: 1017 mean_circ: 0.935154
Ran for 23050 frames Cell Events: 1152 mean_circ: 0.935894
Ran for 23550 frames Cell Events: 1184 mean_circ: 0.935732
Ran for 24550 frames Cell Events: 1238 mean_circ: 0.935866
Ran for 26050 frames Cell Events: 1315 mean_circ: 0.935309
Ran for 26550 frames Cell Events: 1343 mean_circ: 0.935222
Ran for 27050 frames Cell Events: 1375 mean_circ: 0.935201
Ran for 28550 frames Cell Events: 1472 mean_circ: 0.935176
Ran for 29050 frames Cell Events: 1498 mean_circ: 0.934935
Ran for 29550 frames Cell Events: 1523 mean_circ: 0.934755
Ran for 30050 frames Cell Events: 1551 mean_circ: 0.934694
Ran for 31550 frames Cell Events: 1618 mean_circ: 0.93484
Ran for 32550 frames Cell Events: 1664 mean_circ: 0.934861
Ran for 33050 frames Cell Events: 1691 mean_circ: 0.935015
Ran for 33550 frames Cell Events: 1729 mean_circ: 0.934782
Ran for 35550 frames Cell Events: 1800 mean_circ: 0.93502
Ran for 36050 frames Cell Events: 1832 mean_circ: 0.935051
Ran for 37050 frames Cell Events: 1882 mean_circ: 0.93471
Ran for 37550 frames Cell Events: 1906 mean_circ: 0.934758
Ran for 39550 frames Cell Events: 2041 mean_circ: 0.934908
Ran for 40050 frames Cell Events: 2068 mean_circ: 0.934965
Ran for 41050 frames Cell Events: 2114 mean_circ: 0.934942
Ran for 42050 frames Cell Events: 2170 mean_circ: 0.934858
Ran for 42550 frames Cell Events: 2189 mean_circ: 0.934736
Ran for 43050 frames Cell Events: 2221 mean_circ: 0.934824
Ran for 43550 frames Cell Events: 2240 mean_circ: 0.93486
Ran for 44550 frames Cell Events: 2282 mean_circ: 0.935098
Ran for 45050 frames Cell Events: 2304 mean_circ: 0.935054
Ran for 46550 frames Cell Events: 2391 mean_circ: 0.935235
Ran for 47050 frames Cell Events: 2418 mean_circ: 0.935322
Ran for 48050 frames Cell Events: 2458 mean_circ: 0.935219
Ran for 49050 frames Cell Events: 2501 mean_circ: 0.935227
Ran for 50550 frames Cell Events: 2601 mean_circ: 0.935423
Ran for 51050 frames Cell Events: 2624 mean_circ: 0.935458
Ran for 51550 frames Cell Events: 2649 mean_circ: 0.935406
Ran for 52050 frames Cell Events: 2673 mean_circ: 0.935405
Ran for 52550 frames Cell Events: 2689 mean_circ: 0.935474
Ran for 53050 frames Cell Events: 2726 mean_circ: 0.935522
Ran for 53550 frames Cell Events: 2751 mean_circ: 0.935547
Ran for 54050 frames Cell Events: 2779 mean_circ: 0.935467
Ran for 54550 frames Cell Events: 2812 mean_circ: 0.935486
Ran for 55050 frames Cell Events: 2840 mean_circ: 0.93546
Ran for 55550 frames Cell Events: 2861 mean_circ: 0.93547
Ran for 56050 frames Cell Events: 2872 mean_circ: 0.935556
Ran for 56550 frames Cell Events: 2891 mean_circ: 0.935533
Ran for 58550 frames Cell Events: 3011 mean_circ: 0.934943
Ran for 59050 frames Cell Events: 3046 mean_circ: 0.934946
Ran for 59550 frames Cell Events: 3072 mean_circ: 0.935106
Ran for 60550 frames Cell Events: 3124 mean_circ: 0.935078
Ran for 61050 frames Cell Events: 3147 mean_circ: 0.934941
Ran for 62050 frames Cell Events: 3205 mean_circ: 0.935056
Ran for 63050 frames Cell Events: 3265 mean_circ: 0.934951
Ran for 63550 frames Cell Events: 3305 mean_circ: 0.935006
Ran for 64050 frames Cell Events: 3331 mean_circ: 0.935003
Ran for 64550 frames Cell Events: 3355 mean_circ: 0.93493
Ran for 65050 frames Cell Events: 3374 mean_circ: 0.934887
Ran for 66050 frames Cell Events: 3416 mean_circ: 0.934762
Ran for 66550 frames Cell Events: 3439 mean_circ: 0.934804
Ran for 67550 frames Cell Events: 3473 mean_circ: 0.93484
Ran for 68550 frames Cell Events: 3536 mean_circ: 0.934826
Ran for 70050 frames Cell Events: 3629 mean_circ: 0.934781
Ran for 71550 frames Cell Events: 3718 mean_circ: 0.93484
Ran for 73050 frames Cell Events: 3806 mean_circ: 0.934778
Ran for 75550 frames Cell Events: 3964 mean_circ: 0.934279
Ran for 77550 frames Cell Events: 4087 mean_circ: 0.934307
Ran for 78050 frames Cell Events: 4118 mean_circ: 0.934406
Ran for 78550 frames Cell Events: 4144 mean_circ: 0.934444
Ran for 79550 frames Cell Events: 4201 mean_circ: 0.93447
Ran for 80550 frames Cell Events: 4248 mean_circ: 0.934537
Ran for 81550 frames Cell Events: 4303 mean_circ: 0.934471
Ran for 82550 frames Cell Events: 4377 mean_circ: 0.934505
Ran for 83550 frames Cell Events: 4424 mean_circ: 0.934509
Ran for 84050 frames Cell Events: 4455 mean_circ: 0.934482
Ran for 84550 frames Cell Events: 4487 mean_circ: 0.93449
Ran for 86050 frames Cell Events: 4570 mean_circ: 0.934658
Ran for 88050 frames Cell Events: 4690 mean_circ: 0.934633
Ran for 88550 frames Cell Events: 4709 mean_circ: 0.934662
Ran for 89550 frames Cell Events: 4777 mean_circ: 0.934532
Ran for 90550 frames Cell Events: 4822 mean_circ: 0.934484
Ran for 92050 frames Cell Events: 4906 mean_circ: 0.934474
Ran for 92550 frames Cell Events: 4931 mean_circ: 0.934475
Ran for 93050 frames Cell Events: 4961 mean_circ: 0.934501
Ran for 94050 frames Cell Events: 4995 mean_circ: 0.93449
Ran for 94550 frames Cell Events: 5011 mean_circ: 0.934437
Ran for 95050 frames Cell Events: 5053 mean_circ: 0.934383
Ran for 97050 frames Cell Events: 5172 mean_circ: 0.934275
Ran for 97550 frames Cell Events: 5203 mean_circ: 0.934292
Ran for 98050 frames Cell Events: 5230 mean_circ: 0.934315
Ran for 99550 frames Cell Events: 5314 mean_circ: 0.934212
Ran for 100050 frames Cell Events: 5331 mean_circ: 0.934276
Ran for 103050 frames Cell Events: 5476 mean_circ: 0.934184
Ran for 104550 frames Cell Events: 5540 mean_circ: 0.934167
Ran for 105050 frames Cell Events: 5579 mean_circ: 0.934181
Ran for 107050 frames Cell Events: 5701 mean_circ: 0.934261
Ran for 107550 frames Cell Events: 5729 mean_circ: 0.934299
Ran for 108050 frames Cell Events: 5749 mean_circ: 0.934296
Ran for 108550 frames Cell Events: 5772 mean_circ: 0.934185
Ran for 109550 frames Cell Events: 5826 mean_circ: 0.934191
Ran for 110050 frames Cell Events: 5841 mean_circ: 0.934208
Ran for 110550 frames Cell Events: 5861 mean_circ: 0.934191
Ran for 111050 frames Cell Events: 5898 mean_circ: 0.934164
Ran for 111550 frames Cell Events: 5921 mean_circ: 0.934134
Ran for 112050 frames Cell Events: 5948 mean_circ: 0.934099
Ran for 113550 frames Cell Events: 6032 mean_circ: 0.93402
Ran for 114550 frames Cell Events: 6085 mean_circ: 0.934059
Ran for 115050 frames Cell Events: 6119 mean_circ: 0.93392
Ran for 115550 frames Cell Events: 6138 mean_circ: 0.933988
Ran for 116550 frames Cell Events: 6183 mean_circ: 0.933999
Ran for 117050 frames Cell Events: 6201 mean_circ: 0.934066
Ran for 119050 frames Cell Events: 6330 mean_circ: 0.934076
Ran for 120550 frames Cell Events: 6407 mean_circ: 0.934079
Ran for 121050 frames Cell Events: 6428 mean_circ: 0.934135
Ran for 121550 frames Cell Events: 6448 mean_circ: 0.934041
Ran for 122050 frames Cell Events: 6474 mean_circ: 0.933994
Ran for 122550 frames Cell Events: 6494 mean_circ: 0.933954
Ran for 123050 frames Cell Events: 6515 mean_circ: 0.933967
Ran for 124050 frames Cell Events: 6575 mean_circ: 0.933996
Ran for 124550 frames Cell Events: 6609 mean_circ: 0.933996
Ran for 125050 frames Cell Events: 6625 mean_circ: 0.933959
Ran for 127050 frames Cell Events: 6752 mean_circ: 0.933969
Ran for 127550 frames Cell Events: 6768 mean_circ: 0.933972
Ran for 129050 frames Cell Events: 6844 mean_circ: 0.933953
Ran for 129550 frames Cell Events: 6868 mean_circ: 0.933939
Ran for 130050 frames Cell Events: 6887 mean_circ: 0.933962
Ran for 130550 frames Cell Events: 6902 mean_circ: 0.933985
Ran for 131050 frames Cell Events: 6917 mean_circ: 0.934012
Ran for 131550 frames Cell Events: 6940 mean_circ: 0.934022
Ran for 132550 frames Cell Events: 6985 mean_circ: 0.934094
Ran for 133550 frames Cell Events: 7037 mean_circ: 0.934099
Ran for 136050 frames Cell Events: 7160 mean_circ: 0.934119
Ran for 138050 frames Cell Events: 7253 mean_circ: 0.934115
Ran for 138550 frames Cell Events: 7267 mean_circ: 0.934145
Ran for 139050 frames Cell Events: 7302 mean_circ: 0.934132
Ran for 140550 frames Cell Events: 7381 mean_circ: 0.933996
Ran for 141550 frames Cell Events: 7431 mean_circ: 0.934056
Ran for 143050 frames Cell Events: 7491 mean_circ: 0.934042
Ran for 143550 frames Cell Events: 7521 mean_circ: 0.934051
Ran for 144050 frames Cell Events: 7548 mean_circ: 0.934107
Ran for 145050 frames Cell Events: 7587 mean_circ: 0.934066
Ran for 147550 frames Cell Events: 7745 mean_circ: 0.934068
Ran for 148550 frames Cell Events: 7795 mean_circ: 0.933986
Ran for 150050 frames Cell Events: 7873 mean_circ: 0.934075
Ran for 154550 frames Cell Events: 8130 mean_circ: 0.934065
Ran for 155550 frames Cell Events: 8192 mean_circ: 0.934066
Ran for 156050 frames Cell Events: 8214 mean_circ: 0.934062
Ran for 157050 frames Cell Events: 8264 mean_circ: 0.93404



End this measurment..



Stopping time: 2.52099e+007
****************************************
Measurement finished

Mean Circularitiy: 0.933997
Mean Area: 617.729
Mean FL-1 intensity: 3586.74
Mean FL-2 intensity: 5393.64
Mean FL-3 intensity: 0

frames processed: 157533
Total Time: 79600.4ms
Number of Runs (new bg img) 0
Frames collected: 159078
Skipped frames: 38081
Rescued Frames: 1835545
Samples DAQ: 39769436
Samples written: 79538736
Samples per Channel: 39769368
FPS: 1998.46

Samples per second: 499613
Expected samples/s: 500000

Events: 8373
Events per second: 105.188
jumped back (PMT): 10
#Frames read, frequency
# 0 frames :237898
# 1 frames :67463
# 2 frames :3041
# 3 frames :1060
# 4 frames :522
# 5 frames :200
# 6 frames :2274
# 7 frames :1328
# 8 frames :1112
# 9 frames :569
# 10 frames :519
# 11 frames :324
# 12 frames :266
# 13 frames :191
# 14 frames :235
# 15 frames :205
# 16 frames :158
# 17 frames :116
# 18 frames :84
# 19 frames :63
# 20 frames :63
# 21 frames :57
# 22 frames :52
# 23 frames :44
# 24 frames :42
# 25 frames :28
# 26 frames :37
# 27 frames :22
# 28 frames :18
# 29 frames :19
# 30 frames :16
# 31 frames :10
# 32 frames :11
# 33 frames :6
# 34 frames :11
# 35 frames :9
# 36 frames :4
# 37 frames :4
# 38 frames :5
# 39 frames :6
# 40 frames :4
# 41 frames :4
# 42 frames :3
# 43 frames :5
# 44 frames :2
# 45 frames :2
# 46 frames :1
# 47 frames :1
# 48 frames :1
# 49 frames :1
# 50 frames :1
# 52 frames :1
# 54 frames :1
# 56 frames :1
# 58 frames :1
# 61 frames :1
# 62 frames :1
# 67 frames :1
# 68 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.3954
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0