Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e35de0b1e078210e996a2cd0fd6d1103",
"name": "M1_20170515_1658_dsRNA_B8_col.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:16:44"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8137 (max 8138) in '8137'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.2037
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.09407e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 2 mean_circ: 0.930637
Ran for 550 frames Cell Events: 25 mean_circ: 0.953945
Ran for 1550 frames Cell Events: 69 mean_circ: 0.954629
Ran for 2550 frames Cell Events: 120 mean_circ: 0.954685
Ran for 3550 frames Cell Events: 165 mean_circ: 0.953774
Ran for 5050 frames Cell Events: 220 mean_circ: 0.953104
Ran for 6050 frames Cell Events: 281 mean_circ: 0.951703
Ran for 6550 frames Cell Events: 309 mean_circ: 0.950527
Ran for 7050 frames Cell Events: 336 mean_circ: 0.949692
Ran for 9050 frames Cell Events: 435 mean_circ: 0.948572
Ran for 9550 frames Cell Events: 453 mean_circ: 0.948072
Ran for 10050 frames Cell Events: 479 mean_circ: 0.9483
Ran for 10550 frames Cell Events: 499 mean_circ: 0.948455
Ran for 11050 frames Cell Events: 509 mean_circ: 0.948151
Ran for 11550 frames Cell Events: 537 mean_circ: 0.947413
Ran for 12050 frames Cell Events: 561 mean_circ: 0.947585
Ran for 13050 frames Cell Events: 597 mean_circ: 0.946537
Ran for 14050 frames Cell Events: 652 mean_circ: 0.945655
Ran for 14550 frames Cell Events: 674 mean_circ: 0.945698
Ran for 15550 frames Cell Events: 727 mean_circ: 0.946149
Ran for 16550 frames Cell Events: 761 mean_circ: 0.94643
Ran for 17050 frames Cell Events: 788 mean_circ: 0.946252
Ran for 17550 frames Cell Events: 810 mean_circ: 0.946013
Ran for 19050 frames Cell Events: 864 mean_circ: 0.94575
Ran for 19550 frames Cell Events: 882 mean_circ: 0.945852
Ran for 20050 frames Cell Events: 902 mean_circ: 0.944768
Ran for 22550 frames Cell Events: 1005 mean_circ: 0.944727
Ran for 24550 frames Cell Events: 1102 mean_circ: 0.944905
Ran for 25050 frames Cell Events: 1126 mean_circ: 0.945129
Ran for 25550 frames Cell Events: 1144 mean_circ: 0.945059
Ran for 26050 frames Cell Events: 1170 mean_circ: 0.945088
Ran for 26550 frames Cell Events: 1190 mean_circ: 0.945405
Ran for 27550 frames Cell Events: 1247 mean_circ: 0.944949
Ran for 29550 frames Cell Events: 1340 mean_circ: 0.945038
Ran for 30550 frames Cell Events: 1368 mean_circ: 0.945345
Ran for 34050 frames Cell Events: 1548 mean_circ: 0.945948
Ran for 34550 frames Cell Events: 1578 mean_circ: 0.945885
Ran for 35050 frames Cell Events: 1590 mean_circ: 0.945819
Ran for 35550 frames Cell Events: 1618 mean_circ: 0.94573
Ran for 36550 frames Cell Events: 1678 mean_circ: 0.945504
Ran for 37050 frames Cell Events: 1694 mean_circ: 0.945317
Ran for 38550 frames Cell Events: 1761 mean_circ: 0.945299
Ran for 39550 frames Cell Events: 1810 mean_circ: 0.945532
Ran for 40550 frames Cell Events: 1859 mean_circ: 0.945456
Ran for 42050 frames Cell Events: 1917 mean_circ: 0.945605
Ran for 42550 frames Cell Events: 1941 mean_circ: 0.945605
Ran for 43050 frames Cell Events: 1959 mean_circ: 0.945633
Ran for 43550 frames Cell Events: 1977 mean_circ: 0.945799
Ran for 44550 frames Cell Events: 2018 mean_circ: 0.945464
Ran for 45050 frames Cell Events: 2031 mean_circ: 0.945534
Ran for 45550 frames Cell Events: 2052 mean_circ: 0.945633
Ran for 46550 frames Cell Events: 2099 mean_circ: 0.945691
Ran for 48050 frames Cell Events: 2177 mean_circ: 0.945718
Ran for 48550 frames Cell Events: 2211 mean_circ: 0.945604
Ran for 49050 frames Cell Events: 2239 mean_circ: 0.945508
Ran for 51050 frames Cell Events: 2319 mean_circ: 0.945748
Ran for 52050 frames Cell Events: 2342 mean_circ: 0.945694
Ran for 53050 frames Cell Events: 2385 mean_circ: 0.945812
Ran for 54050 frames Cell Events: 2444 mean_circ: 0.945938
Ran for 55050 frames Cell Events: 2491 mean_circ: 0.945953
Ran for 55550 frames Cell Events: 2518 mean_circ: 0.945793
Ran for 56550 frames Cell Events: 2565 mean_circ: 0.945829
Ran for 57050 frames Cell Events: 2583 mean_circ: 0.94563
Ran for 57550 frames Cell Events: 2601 mean_circ: 0.945612
Ran for 58050 frames Cell Events: 2615 mean_circ: 0.945688
Ran for 58550 frames Cell Events: 2628 mean_circ: 0.94566
Ran for 60550 frames Cell Events: 2735 mean_circ: 0.94568
Ran for 61550 frames Cell Events: 2769 mean_circ: 0.945708
Ran for 62550 frames Cell Events: 2817 mean_circ: 0.945705
Ran for 64050 frames Cell Events: 2891 mean_circ: 0.945825
Ran for 64550 frames Cell Events: 2920 mean_circ: 0.945836
Ran for 65050 frames Cell Events: 2948 mean_circ: 0.945781
Ran for 66050 frames Cell Events: 2983 mean_circ: 0.945813
Ran for 67050 frames Cell Events: 3032 mean_circ: 0.945869
Ran for 69050 frames Cell Events: 3111 mean_circ: 0.945961
Ran for 69550 frames Cell Events: 3124 mean_circ: 0.946001
Ran for 70050 frames Cell Events: 3140 mean_circ: 0.945991
Ran for 70550 frames Cell Events: 3164 mean_circ: 0.945981
Ran for 71050 frames Cell Events: 3202 mean_circ: 0.945981
Ran for 71550 frames Cell Events: 3225 mean_circ: 0.94596
Ran for 72050 frames Cell Events: 3242 mean_circ: 0.945988
Ran for 72550 frames Cell Events: 3261 mean_circ: 0.946017
Ran for 73550 frames Cell Events: 3305 mean_circ: 0.946096
Ran for 75050 frames Cell Events: 3374 mean_circ: 0.946172
Ran for 75550 frames Cell Events: 3395 mean_circ: 0.946218
Ran for 76550 frames Cell Events: 3446 mean_circ: 0.946312
Ran for 78550 frames Cell Events: 3537 mean_circ: 0.946388
Ran for 80550 frames Cell Events: 3622 mean_circ: 0.946225
Ran for 81050 frames Cell Events: 3639 mean_circ: 0.946229
Ran for 81550 frames Cell Events: 3665 mean_circ: 0.946193
Ran for 82050 frames Cell Events: 3689 mean_circ: 0.946226
Ran for 83050 frames Cell Events: 3750 mean_circ: 0.946328
Ran for 84050 frames Cell Events: 3800 mean_circ: 0.946404
Ran for 85050 frames Cell Events: 3842 mean_circ: 0.946443
Ran for 86550 frames Cell Events: 3922 mean_circ: 0.946456
Ran for 87550 frames Cell Events: 3964 mean_circ: 0.946536
Ran for 88050 frames Cell Events: 4005 mean_circ: 0.946545
Ran for 88550 frames Cell Events: 4031 mean_circ: 0.946554
Ran for 89050 frames Cell Events: 4062 mean_circ: 0.946596
Ran for 89550 frames Cell Events: 4087 mean_circ: 0.946667
Ran for 91050 frames Cell Events: 4149 mean_circ: 0.946724
Ran for 92050 frames Cell Events: 4186 mean_circ: 0.946726
Ran for 92550 frames Cell Events: 4206 mean_circ: 0.946727
Ran for 93050 frames Cell Events: 4223 mean_circ: 0.946729
Ran for 95050 frames Cell Events: 4322 mean_circ: 0.94675
Ran for 95550 frames Cell Events: 4340 mean_circ: 0.946798
Ran for 98050 frames Cell Events: 4495 mean_circ: 0.947044
Ran for 98550 frames Cell Events: 4511 mean_circ: 0.947071
Ran for 99050 frames Cell Events: 4542 mean_circ: 0.947095
Ran for 99550 frames Cell Events: 4576 mean_circ: 0.947088
Ran for 101550 frames Cell Events: 4706 mean_circ: 0.9471
Ran for 102550 frames Cell Events: 4783 mean_circ: 0.946898
Ran for 103550 frames Cell Events: 4842 mean_circ: 0.94689
Ran for 105050 frames Cell Events: 4949 mean_circ: 0.94678
Ran for 105550 frames Cell Events: 4979 mean_circ: 0.946663
Ran for 107050 frames Cell Events: 5075 mean_circ: 0.9467
Ran for 107550 frames Cell Events: 5105 mean_circ: 0.946714
Ran for 109050 frames Cell Events: 5199 mean_circ: 0.94681
Ran for 110050 frames Cell Events: 5263 mean_circ: 0.946852
Ran for 111050 frames Cell Events: 5323 mean_circ: 0.94695
Ran for 111550 frames Cell Events: 5353 mean_circ: 0.946913
Ran for 113050 frames Cell Events: 5423 mean_circ: 0.94693
Ran for 114550 frames Cell Events: 5498 mean_circ: 0.946874
Ran for 115050 frames Cell Events: 5524 mean_circ: 0.946887
Ran for 117050 frames Cell Events: 5611 mean_circ: 0.946908
Ran for 118050 frames Cell Events: 5666 mean_circ: 0.946913
Ran for 118550 frames Cell Events: 5688 mean_circ: 0.946951
Ran for 119550 frames Cell Events: 5737 mean_circ: 0.946937
Ran for 120050 frames Cell Events: 5763 mean_circ: 0.947004
Ran for 120550 frames Cell Events: 5789 mean_circ: 0.947
Ran for 121550 frames Cell Events: 5838 mean_circ: 0.947063
Ran for 122550 frames Cell Events: 5898 mean_circ: 0.946993
Ran for 123550 frames Cell Events: 5950 mean_circ: 0.947094
Ran for 124050 frames Cell Events: 5972 mean_circ: 0.947075
Ran for 125050 frames Cell Events: 6038 mean_circ: 0.947006
Ran for 125550 frames Cell Events: 6063 mean_circ: 0.94699
Ran for 126050 frames Cell Events: 6090 mean_circ: 0.946971
Ran for 126550 frames Cell Events: 6116 mean_circ: 0.94694
Ran for 127050 frames Cell Events: 6134 mean_circ: 0.94694
Ran for 129050 frames Cell Events: 6237 mean_circ: 0.946919
Ran for 129550 frames Cell Events: 6264 mean_circ: 0.946865
Ran for 130550 frames Cell Events: 6325 mean_circ: 0.946831
Ran for 131050 frames Cell Events: 6358 mean_circ: 0.94687
Ran for 134550 frames Cell Events: 6560 mean_circ: 0.946862
Ran for 135550 frames Cell Events: 6622 mean_circ: 0.946921
Ran for 138050 frames Cell Events: 6756 mean_circ: 0.94691
Ran for 138550 frames Cell Events: 6773 mean_circ: 0.946863
Ran for 139050 frames Cell Events: 6803 mean_circ: 0.946908
Ran for 139550 frames Cell Events: 6823 mean_circ: 0.946871
Ran for 140050 frames Cell Events: 6848 mean_circ: 0.946873
Ran for 141050 frames Cell Events: 6894 mean_circ: 0.946901
Ran for 143050 frames Cell Events: 7003 mean_circ: 0.946923
Ran for 143550 frames Cell Events: 7024 mean_circ: 0.94692
Ran for 144550 frames Cell Events: 7064 mean_circ: 0.946911
Ran for 145050 frames Cell Events: 7086 mean_circ: 0.946909
Ran for 145550 frames Cell Events: 7103 mean_circ: 0.946902
Ran for 146550 frames Cell Events: 7170 mean_circ: 0.946893
Ran for 147550 frames Cell Events: 7219 mean_circ: 0.946838
Ran for 148550 frames Cell Events: 7268 mean_circ: 0.946855
Ran for 149550 frames Cell Events: 7323 mean_circ: 0.946837
Ran for 150550 frames Cell Events: 7377 mean_circ: 0.946846
Ran for 151050 frames Cell Events: 7400 mean_circ: 0.946859
Ran for 152550 frames Cell Events: 7480 mean_circ: 0.946878
Ran for 153050 frames Cell Events: 7502 mean_circ: 0.946882
Ran for 154550 frames Cell Events: 7578 mean_circ: 0.946904
Ran for 156050 frames Cell Events: 7662 mean_circ: 0.946912
Ran for 156550 frames Cell Events: 7687 mean_circ: 0.946921
Ran for 157050 frames Cell Events: 7704 mean_circ: 0.946931
Ran for 157550 frames Cell Events: 7730 mean_circ: 0.946925
Ran for 158050 frames Cell Events: 7755 mean_circ: 0.946927
Ran for 158550 frames Cell Events: 7775 mean_circ: 0.9469
Ran for 162050 frames Cell Events: 7924 mean_circ: 0.94691
Ran for 162550 frames Cell Events: 7943 mean_circ: 0.946921
Ran for 163050 frames Cell Events: 7961 mean_circ: 0.946914
Ran for 164550 frames Cell Events: 8050 mean_circ: 0.946895
Ran for 165050 frames Cell Events: 8073 mean_circ: 0.946915



End this measurment..



Stopping time: 3.10239e+007
****************************************
Measurement finished

Mean Circularitiy: 0.946916
Mean Area: 669.504
Mean FL-1 intensity: 4034.52
Mean FL-2 intensity: 5099.99
Mean FL-3 intensity: 0

frames processed: 165532
Total Time: 83197.6ms
Number of Runs (new bg img) 0
Frames collected: 166264
Skipped frames: 62762
Rescued Frames: 3230823
Samples DAQ: 41567479
Samples written: 83131816
Samples per Channel: 41565908
FPS: 1998.42

Samples per second: 499604
Expected samples/s: 500000

Events: 8138
Events per second: 97.8153
jumped back (PMT): 3073
#Frames read, frequency
# 0 frames :254841
# 1 frames :64198
# 2 frames :2957
# 3 frames :1199
# 4 frames :768
# 5 frames :337
# 6 frames :1020
# 7 frames :1293
# 8 frames :988
# 9 frames :622
# 10 frames :544
# 11 frames :634
# 12 frames :456
# 13 frames :305
# 14 frames :236
# 15 frames :239
# 16 frames :191
# 17 frames :133
# 18 frames :141
# 19 frames :121
# 20 frames :118
# 21 frames :87
# 22 frames :79
# 23 frames :57
# 24 frames :60
# 25 frames :43
# 26 frames :28
# 27 frames :33
# 28 frames :23
# 29 frames :21
# 30 frames :19
# 31 frames :29
# 32 frames :20
# 33 frames :15
# 34 frames :18
# 35 frames :7
# 36 frames :8
# 37 frames :11
# 38 frames :9
# 39 frames :7
# 40 frames :3
# 41 frames :6
# 42 frames :7
# 43 frames :8
# 44 frames :7
# 45 frames :5
# 46 frames :5
# 47 frames :3
# 49 frames :3
# 50 frames :1
# 51 frames :2
# 52 frames :1
# 53 frames :1
# 54 frames :1
# 56 frames :4
# 57 frames :1
# 60 frames :1
# 61 frames :1
# 69 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.2037
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0