Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "09d78f9c63462f1058f3ab4d1c190737",
"name": "M1_20170515_1703_dsRNA_B8_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:17:10"
}
}
dclab-tdms2rtdc-warnings MultipleSamplesPerEventFound
in dclab.rtdc_dataset.fmt_tdms.exc line 101:
Ignoring trace data of
'\\syn03.frontend.storage.int.mpl.mpg.de\guck_division2\Members\Laura\20240219_Kasia_screen_all_folders\20170515_katarzyna_kc167_phospho_screen\20170515_1703_dsRNA_B8_faster\M1_20170515_1703_dsRNA_B8_faster_traces.tdms'
due to multiple values for samples per event: [200 400]
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8349 (max 8350) in '8349'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.2065
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.12387e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 106 mean_circ: 0.935876
Ran for 2050 frames Cell Events: 137 mean_circ: 0.936421
Ran for 2550 frames Cell Events: 160 mean_circ: 0.932573
Ran for 3050 frames Cell Events: 176 mean_circ: 0.93366
Ran for 3550 frames Cell Events: 200 mean_circ: 0.934135
Ran for 4050 frames Cell Events: 227 mean_circ: 0.933581
Ran for 4550 frames Cell Events: 254 mean_circ: 0.933671
Ran for 5050 frames Cell Events: 275 mean_circ: 0.934249
Ran for 6050 frames Cell Events: 321 mean_circ: 0.935153
Ran for 8050 frames Cell Events: 463 mean_circ: 0.935596
Ran for 9050 frames Cell Events: 538 mean_circ: 0.936936
Ran for 10050 frames Cell Events: 586 mean_circ: 0.937358
Ran for 11550 frames Cell Events: 687 mean_circ: 0.936692
Ran for 12050 frames Cell Events: 720 mean_circ: 0.938046
Ran for 12550 frames Cell Events: 757 mean_circ: 0.938408
Ran for 15050 frames Cell Events: 926 mean_circ: 0.938451
Ran for 16550 frames Cell Events: 1007 mean_circ: 0.937988
Ran for 17050 frames Cell Events: 1029 mean_circ: 0.937848
Ran for 17550 frames Cell Events: 1048 mean_circ: 0.937693
Ran for 19550 frames Cell Events: 1180 mean_circ: 0.937026
Ran for 20550 frames Cell Events: 1230 mean_circ: 0.9375
Ran for 21550 frames Cell Events: 1300 mean_circ: 0.937507
Ran for 22550 frames Cell Events: 1344 mean_circ: 0.937631
Ran for 24050 frames Cell Events: 1470 mean_circ: 0.937241
Ran for 24550 frames Cell Events: 1503 mean_circ: 0.937365
Ran for 25550 frames Cell Events: 1580 mean_circ: 0.937629
Ran for 26050 frames Cell Events: 1616 mean_circ: 0.937749
Ran for 26550 frames Cell Events: 1650 mean_circ: 0.937781
Ran for 27050 frames Cell Events: 1680 mean_circ: 0.937817
Ran for 28550 frames Cell Events: 1778 mean_circ: 0.937803
Ran for 29550 frames Cell Events: 1838 mean_circ: 0.937155
Ran for 31050 frames Cell Events: 1942 mean_circ: 0.937094
Ran for 32050 frames Cell Events: 2021 mean_circ: 0.937195
Ran for 33550 frames Cell Events: 2142 mean_circ: 0.937496
Ran for 37050 frames Cell Events: 2429 mean_circ: 0.937816
Ran for 38050 frames Cell Events: 2472 mean_circ: 0.9379
Ran for 38550 frames Cell Events: 2496 mean_circ: 0.938037
Ran for 39050 frames Cell Events: 2522 mean_circ: 0.938101
Ran for 40550 frames Cell Events: 2602 mean_circ: 0.938128
Ran for 41050 frames Cell Events: 2623 mean_circ: 0.938114
Ran for 42550 frames Cell Events: 2712 mean_circ: 0.938108
Ran for 43550 frames Cell Events: 2782 mean_circ: 0.938173
Ran for 44550 frames Cell Events: 2848 mean_circ: 0.938049
Ran for 45550 frames Cell Events: 2900 mean_circ: 0.938009
Ran for 47550 frames Cell Events: 3038 mean_circ: 0.93787
Ran for 48050 frames Cell Events: 3072 mean_circ: 0.937929
Ran for 50550 frames Cell Events: 3247 mean_circ: 0.938023
Ran for 52550 frames Cell Events: 3358 mean_circ: 0.937989
Ran for 53050 frames Cell Events: 3396 mean_circ: 0.937922
Ran for 53550 frames Cell Events: 3417 mean_circ: 0.93795
Ran for 54550 frames Cell Events: 3471 mean_circ: 0.938052
Ran for 55550 frames Cell Events: 3527 mean_circ: 0.937846
Ran for 56550 frames Cell Events: 3593 mean_circ: 0.937696
Ran for 57050 frames Cell Events: 3626 mean_circ: 0.937731
Ran for 58550 frames Cell Events: 3734 mean_circ: 0.937576
Ran for 59050 frames Cell Events: 3774 mean_circ: 0.937638
Ran for 59550 frames Cell Events: 3806 mean_circ: 0.937638
Ran for 60050 frames Cell Events: 3844 mean_circ: 0.937497
Ran for 60550 frames Cell Events: 3874 mean_circ: 0.937597
Ran for 63050 frames Cell Events: 4029 mean_circ: 0.937757
Ran for 64550 frames Cell Events: 4131 mean_circ: 0.937704
Ran for 65050 frames Cell Events: 4158 mean_circ: 0.937743
Ran for 66050 frames Cell Events: 4207 mean_circ: 0.937757
Ran for 67050 frames Cell Events: 4258 mean_circ: 0.937853
Ran for 67550 frames Cell Events: 4294 mean_circ: 0.937873
Ran for 68050 frames Cell Events: 4334 mean_circ: 0.937873
Ran for 70050 frames Cell Events: 4468 mean_circ: 0.937823
Ran for 70550 frames Cell Events: 4507 mean_circ: 0.937725
Ran for 71550 frames Cell Events: 4563 mean_circ: 0.937617
Ran for 72550 frames Cell Events: 4619 mean_circ: 0.937777
Ran for 73050 frames Cell Events: 4641 mean_circ: 0.937717
Ran for 73550 frames Cell Events: 4685 mean_circ: 0.937767
Ran for 75050 frames Cell Events: 4763 mean_circ: 0.937848
Ran for 77050 frames Cell Events: 4853 mean_circ: 0.937907
Ran for 78050 frames Cell Events: 4920 mean_circ: 0.937963
Ran for 79050 frames Cell Events: 4984 mean_circ: 0.937943
Ran for 79550 frames Cell Events: 5015 mean_circ: 0.937977
Ran for 80050 frames Cell Events: 5037 mean_circ: 0.937968
Ran for 80550 frames Cell Events: 5053 mean_circ: 0.937997
Ran for 81550 frames Cell Events: 5115 mean_circ: 0.937995
Ran for 82050 frames Cell Events: 5160 mean_circ: 0.938055
Ran for 82550 frames Cell Events: 5188 mean_circ: 0.938118
Ran for 83050 frames Cell Events: 5217 mean_circ: 0.938133
Ran for 83550 frames Cell Events: 5237 mean_circ: 0.938184
Ran for 84550 frames Cell Events: 5289 mean_circ: 0.93812
Ran for 86050 frames Cell Events: 5389 mean_circ: 0.9381
Ran for 89050 frames Cell Events: 5597 mean_circ: 0.938227
Ran for 89550 frames Cell Events: 5640 mean_circ: 0.938187
Ran for 90050 frames Cell Events: 5668 mean_circ: 0.938171
Ran for 92550 frames Cell Events: 5852 mean_circ: 0.938064
Ran for 94050 frames Cell Events: 5996 mean_circ: 0.937796
Ran for 96550 frames Cell Events: 6187 mean_circ: 0.937879
Ran for 97550 frames Cell Events: 6236 mean_circ: 0.937846
Ran for 98050 frames Cell Events: 6259 mean_circ: 0.937888
Ran for 98550 frames Cell Events: 6284 mean_circ: 0.937915
Ran for 100050 frames Cell Events: 6390 mean_circ: 0.937835
Ran for 101550 frames Cell Events: 6463 mean_circ: 0.937883
Ran for 102050 frames Cell Events: 6501 mean_circ: 0.937895
Ran for 104550 frames Cell Events: 6664 mean_circ: 0.938021
Ran for 105050 frames Cell Events: 6705 mean_circ: 0.937962
Ran for 105550 frames Cell Events: 6730 mean_circ: 0.937948
Ran for 106550 frames Cell Events: 6805 mean_circ: 0.938051
Ran for 107550 frames Cell Events: 6872 mean_circ: 0.938071
Ran for 109050 frames Cell Events: 6965 mean_circ: 0.938131
Ran for 110050 frames Cell Events: 7012 mean_circ: 0.938101
Ran for 111550 frames Cell Events: 7101 mean_circ: 0.938196
Ran for 112550 frames Cell Events: 7162 mean_circ: 0.938204
Ran for 113050 frames Cell Events: 7193 mean_circ: 0.938183
Ran for 114550 frames Cell Events: 7310 mean_circ: 0.938177
Ran for 115050 frames Cell Events: 7328 mean_circ: 0.938129
Ran for 117550 frames Cell Events: 7505 mean_circ: 0.938349
Ran for 118050 frames Cell Events: 7546 mean_circ: 0.938326
Ran for 118550 frames Cell Events: 7584 mean_circ: 0.93831
Ran for 119550 frames Cell Events: 7652 mean_circ: 0.93832
Ran for 121550 frames Cell Events: 7799 mean_circ: 0.938253
Ran for 123050 frames Cell Events: 7878 mean_circ: 0.938199
Ran for 125050 frames Cell Events: 7983 mean_circ: 0.938127
Ran for 127550 frames Cell Events: 8135 mean_circ: 0.938196
Ran for 128550 frames Cell Events: 8182 mean_circ: 0.938105
Ran for 129050 frames Cell Events: 8211 mean_circ: 0.938067
Ran for 130550 frames Cell Events: 8311 mean_circ: 0.938038



End this measurment..



Stopping time: 3.13044e+007
****************************************
Measurement finished

Mean Circularitiy: 0.938055
Mean Area: 664.266
Mean FL-1 intensity: 3797.24
Mean FL-2 intensity: 5576.75
Mean FL-3 intensity: 0

frames processed: 128110
Total Time: 65753.5ms
Number of Runs (new bg img) 0
Frames collected: 131339
Skipped frames: 65992
Rescued Frames: 3309419
Samples DAQ: 32836706
Samples written: 65669278
Samples per Channel: 32834639
FPS: 1997.44

Samples per second: 499359
Expected samples/s: 500000

Events: 8351
Events per second: 127.005
jumped back (PMT): 30
#Frames read, frequency
# 0 frames :213098
# 1 frames :38907
# 2 frames :2276
# 3 frames :805
# 4 frames :520
# 5 frames :223
# 6 frames :1315
# 7 frames :937
# 8 frames :773
# 9 frames :579
# 10 frames :545
# 11 frames :376
# 12 frames :283
# 13 frames :176
# 14 frames :190
# 15 frames :198
# 16 frames :201
# 17 frames :171
# 18 frames :138
# 19 frames :94
# 20 frames :78
# 21 frames :91
# 22 frames :79
# 23 frames :64
# 24 frames :61
# 25 frames :44
# 26 frames :46
# 27 frames :46
# 28 frames :31
# 29 frames :38
# 30 frames :25
# 31 frames :25
# 32 frames :22
# 33 frames :26
# 34 frames :21
# 35 frames :15
# 36 frames :11
# 37 frames :8
# 38 frames :10
# 39 frames :15
# 40 frames :3
# 41 frames :8
# 42 frames :5
# 43 frames :5
# 44 frames :6
# 45 frames :5
# 46 frames :7
# 47 frames :3
# 48 frames :2
# 49 frames :3
# 50 frames :4
# 51 frames :5
# 52 frames :5
# 53 frames :3
# 54 frames :3
# 55 frames :3
# 56 frames :1
# 57 frames :2
# 58 frames :4
# 59 frames :3
# 60 frames :1
# 61 frames :1
# 64 frames :1
# 66 frames :1
# 69 frames :1
# 85 frames :1
# 95 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.2065
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0