Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ef638b4441f2848afc66672f5ef5ba9e",
"name": "M1_20170515_1730_dsRNA_c1_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:19:09"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8101 (max 8102) in '8101'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.2298
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.28638e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 116 mean_circ: 0.942952
Ran for 2550 frames Cell Events: 136 mean_circ: 0.938927
Ran for 4050 frames Cell Events: 204 mean_circ: 0.937302
Ran for 4550 frames Cell Events: 224 mean_circ: 0.936923
Ran for 5050 frames Cell Events: 252 mean_circ: 0.9362
Ran for 6550 frames Cell Events: 306 mean_circ: 0.938137
Ran for 9050 frames Cell Events: 396 mean_circ: 0.937189
Ran for 9550 frames Cell Events: 419 mean_circ: 0.937217
Ran for 10050 frames Cell Events: 436 mean_circ: 0.937114
Ran for 10550 frames Cell Events: 461 mean_circ: 0.936813
Ran for 12050 frames Cell Events: 556 mean_circ: 0.937309
Ran for 12550 frames Cell Events: 575 mean_circ: 0.936908
Ran for 13050 frames Cell Events: 601 mean_circ: 0.936754
Ran for 13550 frames Cell Events: 619 mean_circ: 0.936829
Ran for 17050 frames Cell Events: 763 mean_circ: 0.937119
Ran for 17550 frames Cell Events: 786 mean_circ: 0.936788
Ran for 18050 frames Cell Events: 824 mean_circ: 0.936471
Ran for 19050 frames Cell Events: 845 mean_circ: 0.936022
Ran for 19550 frames Cell Events: 865 mean_circ: 0.936002
Ran for 20050 frames Cell Events: 897 mean_circ: 0.935959
Ran for 21050 frames Cell Events: 958 mean_circ: 0.935012
Ran for 21550 frames Cell Events: 983 mean_circ: 0.934938
Ran for 23050 frames Cell Events: 1074 mean_circ: 0.934968
Ran for 23550 frames Cell Events: 1099 mean_circ: 0.934926
Ran for 24550 frames Cell Events: 1142 mean_circ: 0.934926
Ran for 26550 frames Cell Events: 1242 mean_circ: 0.933292
Ran for 27550 frames Cell Events: 1295 mean_circ: 0.933443
Ran for 28050 frames Cell Events: 1330 mean_circ: 0.933724
Ran for 28550 frames Cell Events: 1363 mean_circ: 0.933776
Ran for 29050 frames Cell Events: 1380 mean_circ: 0.933795
Ran for 29550 frames Cell Events: 1409 mean_circ: 0.933613
Ran for 31050 frames Cell Events: 1503 mean_circ: 0.933302
Ran for 31550 frames Cell Events: 1525 mean_circ: 0.933315
Ran for 32050 frames Cell Events: 1548 mean_circ: 0.933289
Ran for 33050 frames Cell Events: 1603 mean_circ: 0.933068
Ran for 33550 frames Cell Events: 1624 mean_circ: 0.933036
Ran for 34050 frames Cell Events: 1652 mean_circ: 0.933064
Ran for 36050 frames Cell Events: 1759 mean_circ: 0.933306
Ran for 36550 frames Cell Events: 1793 mean_circ: 0.933371
Ran for 37050 frames Cell Events: 1813 mean_circ: 0.933101
Ran for 38050 frames Cell Events: 1869 mean_circ: 0.93309
Ran for 41050 frames Cell Events: 2039 mean_circ: 0.933259
Ran for 42050 frames Cell Events: 2090 mean_circ: 0.9334
Ran for 42550 frames Cell Events: 2118 mean_circ: 0.93314
Ran for 43050 frames Cell Events: 2137 mean_circ: 0.933311
Ran for 43550 frames Cell Events: 2155 mean_circ: 0.93329
Ran for 44050 frames Cell Events: 2183 mean_circ: 0.933393
Ran for 44550 frames Cell Events: 2221 mean_circ: 0.933407
Ran for 45550 frames Cell Events: 2283 mean_circ: 0.932943
Ran for 46050 frames Cell Events: 2306 mean_circ: 0.932896
Ran for 46550 frames Cell Events: 2339 mean_circ: 0.932772
Ran for 47550 frames Cell Events: 2411 mean_circ: 0.932852
Ran for 51050 frames Cell Events: 2622 mean_circ: 0.933166
Ran for 51550 frames Cell Events: 2660 mean_circ: 0.933312
Ran for 52050 frames Cell Events: 2683 mean_circ: 0.9332
Ran for 53050 frames Cell Events: 2748 mean_circ: 0.933408
Ran for 53550 frames Cell Events: 2779 mean_circ: 0.933262
Ran for 54550 frames Cell Events: 2842 mean_circ: 0.933276
Ran for 55050 frames Cell Events: 2881 mean_circ: 0.933229
Ran for 55550 frames Cell Events: 2908 mean_circ: 0.933223
Ran for 56050 frames Cell Events: 2930 mean_circ: 0.933319
Ran for 57050 frames Cell Events: 2979 mean_circ: 0.933305
Ran for 57550 frames Cell Events: 3011 mean_circ: 0.933287
Ran for 58050 frames Cell Events: 3037 mean_circ: 0.933367
Ran for 59050 frames Cell Events: 3100 mean_circ: 0.93341
Ran for 59550 frames Cell Events: 3120 mean_circ: 0.933426
Ran for 60050 frames Cell Events: 3151 mean_circ: 0.933459
Ran for 61050 frames Cell Events: 3222 mean_circ: 0.93328
Ran for 61550 frames Cell Events: 3252 mean_circ: 0.933268
Ran for 62550 frames Cell Events: 3311 mean_circ: 0.933087
Ran for 63050 frames Cell Events: 3339 mean_circ: 0.933122
Ran for 63550 frames Cell Events: 3366 mean_circ: 0.933212
Ran for 64050 frames Cell Events: 3398 mean_circ: 0.933245
Ran for 65050 frames Cell Events: 3485 mean_circ: 0.932892
Ran for 65550 frames Cell Events: 3507 mean_circ: 0.932921
Ran for 66050 frames Cell Events: 3541 mean_circ: 0.932798
Ran for 66550 frames Cell Events: 3573 mean_circ: 0.932828
Ran for 67050 frames Cell Events: 3616 mean_circ: 0.932854
Ran for 67550 frames Cell Events: 3652 mean_circ: 0.932876
Ran for 68050 frames Cell Events: 3679 mean_circ: 0.932831
Ran for 70550 frames Cell Events: 3825 mean_circ: 0.932865
Ran for 71050 frames Cell Events: 3848 mean_circ: 0.932949
Ran for 71550 frames Cell Events: 3887 mean_circ: 0.932809
Ran for 72550 frames Cell Events: 3945 mean_circ: 0.932855
Ran for 73550 frames Cell Events: 3993 mean_circ: 0.932662
Ran for 74550 frames Cell Events: 4044 mean_circ: 0.932638
Ran for 75550 frames Cell Events: 4101 mean_circ: 0.932642
Ran for 76550 frames Cell Events: 4181 mean_circ: 0.932659
Ran for 77050 frames Cell Events: 4200 mean_circ: 0.932647
Ran for 77550 frames Cell Events: 4223 mean_circ: 0.932615
Ran for 79050 frames Cell Events: 4298 mean_circ: 0.932484
Ran for 80050 frames Cell Events: 4351 mean_circ: 0.932473
Ran for 81050 frames Cell Events: 4412 mean_circ: 0.932619
Ran for 81550 frames Cell Events: 4441 mean_circ: 0.932582
Ran for 82550 frames Cell Events: 4493 mean_circ: 0.932314
Ran for 83050 frames Cell Events: 4520 mean_circ: 0.932337
Ran for 83550 frames Cell Events: 4542 mean_circ: 0.932288
Ran for 84550 frames Cell Events: 4619 mean_circ: 0.932275
Ran for 85050 frames Cell Events: 4638 mean_circ: 0.932398
Ran for 85550 frames Cell Events: 4659 mean_circ: 0.932451
Ran for 86050 frames Cell Events: 4684 mean_circ: 0.932465
Ran for 87550 frames Cell Events: 4769 mean_circ: 0.932532
Ran for 88050 frames Cell Events: 4784 mean_circ: 0.932559
Ran for 88550 frames Cell Events: 4810 mean_circ: 0.932555
Ran for 89550 frames Cell Events: 4872 mean_circ: 0.93248
Ran for 90550 frames Cell Events: 4924 mean_circ: 0.932386
Ran for 92050 frames Cell Events: 5021 mean_circ: 0.932396
Ran for 92550 frames Cell Events: 5043 mean_circ: 0.932255
Ran for 93550 frames Cell Events: 5098 mean_circ: 0.932163
Ran for 94050 frames Cell Events: 5130 mean_circ: 0.93215
Ran for 95550 frames Cell Events: 5203 mean_circ: 0.932173
Ran for 96050 frames Cell Events: 5244 mean_circ: 0.932037
Ran for 99550 frames Cell Events: 5467 mean_circ: 0.931986
Ran for 100050 frames Cell Events: 5496 mean_circ: 0.932061
Ran for 101050 frames Cell Events: 5552 mean_circ: 0.932019
Ran for 102550 frames Cell Events: 5639 mean_circ: 0.932025
Ran for 103050 frames Cell Events: 5661 mean_circ: 0.931943
Ran for 103550 frames Cell Events: 5681 mean_circ: 0.931913
Ran for 105050 frames Cell Events: 5767 mean_circ: 0.931913
Ran for 106550 frames Cell Events: 5866 mean_circ: 0.931916
Ran for 107050 frames Cell Events: 5891 mean_circ: 0.93194
Ran for 107550 frames Cell Events: 5917 mean_circ: 0.931958
Ran for 108550 frames Cell Events: 5971 mean_circ: 0.931987
Ran for 109050 frames Cell Events: 5998 mean_circ: 0.932025
Ran for 109550 frames Cell Events: 6022 mean_circ: 0.932023
Ran for 111050 frames Cell Events: 6126 mean_circ: 0.931966
Ran for 111550 frames Cell Events: 6156 mean_circ: 0.931965
Ran for 112050 frames Cell Events: 6177 mean_circ: 0.931995
Ran for 112550 frames Cell Events: 6203 mean_circ: 0.932043
Ran for 113050 frames Cell Events: 6222 mean_circ: 0.931985
Ran for 114050 frames Cell Events: 6266 mean_circ: 0.931967
Ran for 116050 frames Cell Events: 6384 mean_circ: 0.931874
Ran for 116550 frames Cell Events: 6413 mean_circ: 0.931885
Ran for 117550 frames Cell Events: 6480 mean_circ: 0.931979
Ran for 118550 frames Cell Events: 6545 mean_circ: 0.93197
Ran for 119550 frames Cell Events: 6582 mean_circ: 0.932033
Ran for 120050 frames Cell Events: 6608 mean_circ: 0.931966
Ran for 120550 frames Cell Events: 6632 mean_circ: 0.93201
Ran for 121050 frames Cell Events: 6654 mean_circ: 0.932018
Ran for 121550 frames Cell Events: 6685 mean_circ: 0.932035
Ran for 122050 frames Cell Events: 6710 mean_circ: 0.932049
Ran for 122550 frames Cell Events: 6738 mean_circ: 0.932081
Ran for 123050 frames Cell Events: 6760 mean_circ: 0.932069
Ran for 124550 frames Cell Events: 6840 mean_circ: 0.932139
Ran for 126050 frames Cell Events: 6936 mean_circ: 0.932072
Ran for 127050 frames Cell Events: 6979 mean_circ: 0.931996
Ran for 128050 frames Cell Events: 7043 mean_circ: 0.931979
Ran for 129550 frames Cell Events: 7130 mean_circ: 0.931853
Ran for 131050 frames Cell Events: 7236 mean_circ: 0.931835
Ran for 131550 frames Cell Events: 7254 mean_circ: 0.931908
Ran for 132050 frames Cell Events: 7278 mean_circ: 0.931878
Ran for 132550 frames Cell Events: 7307 mean_circ: 0.931874
Ran for 133050 frames Cell Events: 7325 mean_circ: 0.931926
Ran for 133550 frames Cell Events: 7353 mean_circ: 0.931924
Ran for 137050 frames Cell Events: 7558 mean_circ: 0.931866
Ran for 137550 frames Cell Events: 7589 mean_circ: 0.931887
Ran for 138550 frames Cell Events: 7631 mean_circ: 0.931893
Ran for 139550 frames Cell Events: 7694 mean_circ: 0.931829
Ran for 140550 frames Cell Events: 7754 mean_circ: 0.931845
Ran for 141050 frames Cell Events: 7772 mean_circ: 0.931838
Ran for 142050 frames Cell Events: 7841 mean_circ: 0.931849
Ran for 142550 frames Cell Events: 7869 mean_circ: 0.931828
Ran for 143050 frames Cell Events: 7893 mean_circ: 0.931813
Ran for 144550 frames Cell Events: 7990 mean_circ: 0.931773
Ran for 145050 frames Cell Events: 8022 mean_circ: 0.931805
Ran for 145550 frames Cell Events: 8045 mean_circ: 0.931793



End this measurment..



Stopping time: 3.29371e+007
****************************************
Measurement finished

Mean Circularitiy: 0.931832
Mean Area: 674.765
Mean FL-1 intensity: 3327.46
Mean FL-2 intensity: 5369.76
Mean FL-3 intensity: 0

frames processed: 142380
Total Time: 73309.5ms
Number of Runs (new bg img) 0
Frames collected: 146463
Skipped frames: 75893
Rescued Frames: 3679122
Samples DAQ: 36615696
Samples written: 73231280
Samples per Channel: 36615640
FPS: 1997.87

Samples per second: 499466
Expected samples/s: 500000

Events: 8102
Events per second: 110.518
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :221420
# 1 frames :59747
# 2 frames :2775
# 3 frames :891
# 4 frames :451
# 5 frames :198
# 6 frames :2331
# 7 frames :1050
# 8 frames :1158
# 9 frames :588
# 10 frames :382
# 11 frames :249
# 12 frames :247
# 13 frames :138
# 14 frames :229
# 15 frames :203
# 16 frames :139
# 17 frames :117
# 18 frames :71
# 19 frames :54
# 20 frames :62
# 21 frames :41
# 22 frames :58
# 23 frames :47
# 24 frames :34
# 25 frames :26
# 26 frames :25
# 27 frames :18
# 28 frames :15
# 29 frames :15
# 30 frames :18
# 31 frames :8
# 32 frames :11
# 33 frames :5
# 34 frames :4
# 35 frames :4
# 36 frames :8
# 37 frames :5
# 38 frames :4
# 39 frames :5
# 40 frames :5
# 41 frames :2
# 42 frames :4
# 43 frames :1
# 44 frames :3
# 45 frames :5
# 46 frames :2
# 47 frames :5
# 48 frames :1
# 49 frames :2
# 52 frames :2
# 53 frames :1
# 55 frames :2
# 65 frames :1
# 81 frames :1
# 82 frames :1
# 87 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.2298
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0