Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b9a28b5c4a53dd89435c83b422f32eda",
"name": "M1_20170515_1832_dsRNA_C6_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:23:48"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.2126
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.65873e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 26 mean_circ: 0.936329
Ran for 2050 frames Cell Events: 104 mean_circ: 0.938282
Ran for 2550 frames Cell Events: 126 mean_circ: 0.937143
Ran for 3050 frames Cell Events: 151 mean_circ: 0.936263
Ran for 4550 frames Cell Events: 243 mean_circ: 0.93503
Ran for 6050 frames Cell Events: 316 mean_circ: 0.936192
Ran for 6550 frames Cell Events: 343 mean_circ: 0.936748
Ran for 8550 frames Cell Events: 453 mean_circ: 0.935763
Ran for 9050 frames Cell Events: 479 mean_circ: 0.935202
Ran for 9550 frames Cell Events: 508 mean_circ: 0.935244
Ran for 11050 frames Cell Events: 586 mean_circ: 0.936425
Ran for 11550 frames Cell Events: 614 mean_circ: 0.935916
Ran for 12050 frames Cell Events: 644 mean_circ: 0.935107
Ran for 13050 frames Cell Events: 712 mean_circ: 0.934607
Ran for 16550 frames Cell Events: 872 mean_circ: 0.932569
Ran for 18050 frames Cell Events: 950 mean_circ: 0.932876
Ran for 18550 frames Cell Events: 981 mean_circ: 0.933005
Ran for 20050 frames Cell Events: 1083 mean_circ: 0.93278
Ran for 21550 frames Cell Events: 1195 mean_circ: 0.933838
Ran for 22050 frames Cell Events: 1226 mean_circ: 0.93357
Ran for 22550 frames Cell Events: 1244 mean_circ: 0.933804
Ran for 24050 frames Cell Events: 1315 mean_circ: 0.93373
Ran for 25050 frames Cell Events: 1360 mean_circ: 0.933881
Ran for 25550 frames Cell Events: 1390 mean_circ: 0.934183
Ran for 26050 frames Cell Events: 1415 mean_circ: 0.934021
Ran for 26550 frames Cell Events: 1454 mean_circ: 0.934229
Ran for 27550 frames Cell Events: 1507 mean_circ: 0.933578
Ran for 28050 frames Cell Events: 1524 mean_circ: 0.933639
Ran for 30550 frames Cell Events: 1673 mean_circ: 0.934567
Ran for 33050 frames Cell Events: 1828 mean_circ: 0.934525
Ran for 33550 frames Cell Events: 1856 mean_circ: 0.934463
Ran for 35050 frames Cell Events: 1952 mean_circ: 0.934293
Ran for 35550 frames Cell Events: 1983 mean_circ: 0.934417
Ran for 36050 frames Cell Events: 2014 mean_circ: 0.934413
Ran for 37050 frames Cell Events: 2060 mean_circ: 0.934396
Ran for 37550 frames Cell Events: 2092 mean_circ: 0.934447
Ran for 38050 frames Cell Events: 2117 mean_circ: 0.934488
Ran for 38550 frames Cell Events: 2139 mean_circ: 0.934422
Ran for 40550 frames Cell Events: 2267 mean_circ: 0.934223
Ran for 41050 frames Cell Events: 2301 mean_circ: 0.934116
Ran for 41550 frames Cell Events: 2326 mean_circ: 0.934252
Ran for 42050 frames Cell Events: 2349 mean_circ: 0.934349
Ran for 43050 frames Cell Events: 2397 mean_circ: 0.934375
Ran for 43550 frames Cell Events: 2427 mean_circ: 0.934465
Ran for 44550 frames Cell Events: 2487 mean_circ: 0.934451
Ran for 45050 frames Cell Events: 2511 mean_circ: 0.934507
Ran for 45550 frames Cell Events: 2544 mean_circ: 0.934471
Ran for 48550 frames Cell Events: 2705 mean_circ: 0.934457
Ran for 49050 frames Cell Events: 2729 mean_circ: 0.934336
Ran for 49550 frames Cell Events: 2757 mean_circ: 0.93432
Ran for 50050 frames Cell Events: 2790 mean_circ: 0.934449
Ran for 50550 frames Cell Events: 2822 mean_circ: 0.934406
Ran for 51550 frames Cell Events: 2867 mean_circ: 0.93442
Ran for 53050 frames Cell Events: 2963 mean_circ: 0.934596
Ran for 53550 frames Cell Events: 2994 mean_circ: 0.934781
Ran for 54050 frames Cell Events: 3010 mean_circ: 0.934849
Ran for 54550 frames Cell Events: 3046 mean_circ: 0.934849
Ran for 55550 frames Cell Events: 3107 mean_circ: 0.935146
Ran for 56050 frames Cell Events: 3130 mean_circ: 0.935218
Ran for 56550 frames Cell Events: 3157 mean_circ: 0.935058
Ran for 57550 frames Cell Events: 3218 mean_circ: 0.934849
Ran for 58050 frames Cell Events: 3249 mean_circ: 0.934696
Ran for 58550 frames Cell Events: 3272 mean_circ: 0.934804
Ran for 59050 frames Cell Events: 3298 mean_circ: 0.934802
Ran for 61050 frames Cell Events: 3394 mean_circ: 0.934942
Ran for 61550 frames Cell Events: 3427 mean_circ: 0.934942
Ran for 62550 frames Cell Events: 3473 mean_circ: 0.934802
Ran for 63050 frames Cell Events: 3489 mean_circ: 0.934755
Ran for 63550 frames Cell Events: 3515 mean_circ: 0.934764
Ran for 65050 frames Cell Events: 3623 mean_circ: 0.934743
Ran for 65550 frames Cell Events: 3661 mean_circ: 0.934746
Ran for 66050 frames Cell Events: 3678 mean_circ: 0.934691
Ran for 70550 frames Cell Events: 3923 mean_circ: 0.934833
Ran for 71050 frames Cell Events: 3945 mean_circ: 0.934835
Ran for 73050 frames Cell Events: 4057 mean_circ: 0.934752
Ran for 75050 frames Cell Events: 4173 mean_circ: 0.934893
Ran for 75550 frames Cell Events: 4207 mean_circ: 0.934935
Ran for 76050 frames Cell Events: 4228 mean_circ: 0.934954
Ran for 76550 frames Cell Events: 4253 mean_circ: 0.934867
Ran for 77050 frames Cell Events: 4273 mean_circ: 0.934866
Ran for 77550 frames Cell Events: 4305 mean_circ: 0.934885
Ran for 81550 frames Cell Events: 4528 mean_circ: 0.934978
Ran for 82050 frames Cell Events: 4547 mean_circ: 0.935009
Ran for 84550 frames Cell Events: 4722 mean_circ: 0.935082
Ran for 86550 frames Cell Events: 4863 mean_circ: 0.935173
Ran for 87050 frames Cell Events: 4885 mean_circ: 0.935174
Ran for 87550 frames Cell Events: 4905 mean_circ: 0.935181
Ran for 88050 frames Cell Events: 4933 mean_circ: 0.935177
Ran for 89050 frames Cell Events: 4993 mean_circ: 0.935092
Ran for 91550 frames Cell Events: 5126 mean_circ: 0.935068
Ran for 92050 frames Cell Events: 5161 mean_circ: 0.935058
Ran for 92550 frames Cell Events: 5189 mean_circ: 0.93509
Ran for 94050 frames Cell Events: 5271 mean_circ: 0.935081
Ran for 95050 frames Cell Events: 5333 mean_circ: 0.934986
Ran for 95550 frames Cell Events: 5370 mean_circ: 0.934995
Ran for 96050 frames Cell Events: 5388 mean_circ: 0.934995
Ran for 96550 frames Cell Events: 5415 mean_circ: 0.934951
Ran for 97050 frames Cell Events: 5445 mean_circ: 0.934947
Ran for 97550 frames Cell Events: 5472 mean_circ: 0.935026
Ran for 98550 frames Cell Events: 5519 mean_circ: 0.934914
Ran for 99550 frames Cell Events: 5589 mean_circ: 0.934888
Ran for 100050 frames Cell Events: 5620 mean_circ: 0.934808
Ran for 102050 frames Cell Events: 5718 mean_circ: 0.934871
Ran for 103050 frames Cell Events: 5773 mean_circ: 0.934894
Ran for 104050 frames Cell Events: 5836 mean_circ: 0.934874
Ran for 104550 frames Cell Events: 5858 mean_circ: 0.934787
Ran for 105550 frames Cell Events: 5913 mean_circ: 0.934732
Ran for 106050 frames Cell Events: 5926 mean_circ: 0.934697
Ran for 106550 frames Cell Events: 5951 mean_circ: 0.934701
Ran for 107550 frames Cell Events: 6010 mean_circ: 0.934772
Ran for 108050 frames Cell Events: 6039 mean_circ: 0.934785
Ran for 108550 frames Cell Events: 6058 mean_circ: 0.934799
Ran for 110050 frames Cell Events: 6157 mean_circ: 0.9349
Ran for 111050 frames Cell Events: 6206 mean_circ: 0.934987
Ran for 112050 frames Cell Events: 6269 mean_circ: 0.935045
Ran for 112550 frames Cell Events: 6311 mean_circ: 0.935018
Ran for 113050 frames Cell Events: 6324 mean_circ: 0.935063
Ran for 114050 frames Cell Events: 6380 mean_circ: 0.935051
Ran for 114550 frames Cell Events: 6408 mean_circ: 0.93506
Ran for 116050 frames Cell Events: 6501 mean_circ: 0.934902
Ran for 117050 frames Cell Events: 6564 mean_circ: 0.934929
Ran for 117550 frames Cell Events: 6584 mean_circ: 0.934874
Ran for 118050 frames Cell Events: 6617 mean_circ: 0.93487
Ran for 118550 frames Cell Events: 6654 mean_circ: 0.934887
Ran for 119050 frames Cell Events: 6674 mean_circ: 0.934814
Ran for 122050 frames Cell Events: 6866 mean_circ: 0.934629
Ran for 124050 frames Cell Events: 6988 mean_circ: 0.934495
Ran for 125550 frames Cell Events: 7087 mean_circ: 0.934456
Ran for 126550 frames Cell Events: 7160 mean_circ: 0.934364
Ran for 127050 frames Cell Events: 7192 mean_circ: 0.934401
Ran for 127550 frames Cell Events: 7218 mean_circ: 0.934418
Ran for 128550 frames Cell Events: 7279 mean_circ: 0.934331
Ran for 129050 frames Cell Events: 7299 mean_circ: 0.934326
Ran for 132550 frames Cell Events: 7497 mean_circ: 0.934355
Ran for 133050 frames Cell Events: 7516 mean_circ: 0.934306
Ran for 133550 frames Cell Events: 7541 mean_circ: 0.93432
Ran for 135550 frames Cell Events: 7646 mean_circ: 0.934221
Ran for 136050 frames Cell Events: 7669 mean_circ: 0.934241
Ran for 137550 frames Cell Events: 7738 mean_circ: 0.934307
Ran for 138050 frames Cell Events: 7765 mean_circ: 0.934238
Ran for 138550 frames Cell Events: 7780 mean_circ: 0.934241
Ran for 141050 frames Cell Events: 7940 mean_circ: 0.93432
Ran for 142050 frames Cell Events: 8019 mean_circ: 0.93439
Ran for 142550 frames Cell Events: 8059 mean_circ: 0.934338
Ran for 143050 frames Cell Events: 8105 mean_circ: 0.934296
Ran for 144550 frames Cell Events: 8195 mean_circ: 0.934214
Ran for 145550 frames Cell Events: 8260 mean_circ: 0.934176
Ran for 147050 frames Cell Events: 8337 mean_circ: 0.934127
Ran for 148050 frames Cell Events: 8406 mean_circ: 0.934247
Ran for 149550 frames Cell Events: 8521 mean_circ: 0.93421
Ran for 150050 frames Cell Events: 8543 mean_circ: 0.93426
Ran for 150550 frames Cell Events: 8574 mean_circ: 0.934253
Ran for 151050 frames Cell Events: 8605 mean_circ: 0.934226
Ran for 151550 frames Cell Events: 8643 mean_circ: 0.934183
Ran for 152050 frames Cell Events: 8662 mean_circ: 0.934197
Ran for 152550 frames Cell Events: 8683 mean_circ: 0.934178
Ran for 153050 frames Cell Events: 8717 mean_circ: 0.934144
Ran for 154050 frames Cell Events: 8785 mean_circ: 0.934108
Ran for 154550 frames Cell Events: 8811 mean_circ: 0.934083
Ran for 155050 frames Cell Events: 8843 mean_circ: 0.934122
Ran for 156050 frames Cell Events: 8911 mean_circ: 0.934137
Ran for 157550 frames Cell Events: 8991 mean_circ: 0.934152
Ran for 158550 frames Cell Events: 9043 mean_circ: 0.934142
Ran for 159050 frames Cell Events: 9072 mean_circ: 0.934144
Ran for 160050 frames Cell Events: 9129 mean_circ: 0.934128
Ran for 160550 frames Cell Events: 9159 mean_circ: 0.934153
Ran for 162050 frames Cell Events: 9250 mean_circ: 0.934097
Ran for 163550 frames Cell Events: 9336 mean_circ: 0.934185
Ran for 164050 frames Cell Events: 9369 mean_circ: 0.93412
Ran for 164550 frames Cell Events: 9390 mean_circ: 0.934118
Ran for 165550 frames Cell Events: 9440 mean_circ: 0.934092
Ran for 166550 frames Cell Events: 9484 mean_circ: 0.934075
Ran for 167550 frames Cell Events: 9541 mean_circ: 0.934106
Ran for 168050 frames Cell Events: 9565 mean_circ: 0.934141
Ran for 168550 frames Cell Events: 9593 mean_circ: 0.93416
Ran for 169050 frames Cell Events: 9622 mean_circ: 0.93421
Ran for 170050 frames Cell Events: 9683 mean_circ: 0.934104
Ran for 171050 frames Cell Events: 9740 mean_circ: 0.93408
Ran for 171550 frames Cell Events: 9764 mean_circ: 0.934068
Ran for 174050 frames Cell Events: 9887 mean_circ: 0.934066
Ran for 174550 frames Cell Events: 9920 mean_circ: 0.934081



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 3.66754e+007
****************************************
Measurement finished

Mean Circularitiy: 0.934079
Mean Area: 649.324
Mean FL-1 intensity: 3622.84
Mean FL-2 intensity: 5474.01
Mean FL-3 intensity: 0

frames processed: 171673
Total Time: 88076.9ms
Number of Runs (new bg img) 0
Frames collected: 176010
Skipped frames: 97423
Rescued Frames: 4558028
Samples DAQ: 44007909
Samples written: 88005030
Samples per Channel: 44002515
FPS: 1998.37

Samples per second: 499592
Expected samples/s: 500000

Events: 10002
Events per second: 113.56
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :267622
# 1 frames :70387
# 2 frames :3138
# 3 frames :1035
# 4 frames :518
# 5 frames :271
# 6 frames :2696
# 7 frames :1358
# 8 frames :1246
# 9 frames :610
# 10 frames :448
# 11 frames :287
# 12 frames :306
# 13 frames :237
# 14 frames :310
# 15 frames :246
# 16 frames :184
# 17 frames :142
# 18 frames :91
# 19 frames :110
# 20 frames :97
# 21 frames :63
# 22 frames :64
# 23 frames :55
# 24 frames :50
# 25 frames :54
# 26 frames :33
# 27 frames :27
# 28 frames :31
# 29 frames :15
# 30 frames :11
# 31 frames :21
# 32 frames :19
# 33 frames :12
# 34 frames :9
# 35 frames :9
# 36 frames :6
# 37 frames :3
# 38 frames :6
# 39 frames :4
# 40 frames :6
# 41 frames :2
# 43 frames :3
# 44 frames :5
# 45 frames :3
# 46 frames :1
# 47 frames :3
# 48 frames :2
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 55 frames :3
# 56 frames :4
# 58 frames :1
# 59 frames :2
# 63 frames :1
# 67 frames :1
# 68 frames :1
# 73 frames :1
# 80 frames :1
# 95 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.2126
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0