Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "295fe82bbfee040d84a2e91ccba10537",
"name": "M1_20170515_1855_dsRNA_C8_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:25:46"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.3398
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.79801e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.944683
Ran for 550 frames Cell Events: 24 mean_circ: 0.921459
Ran for 1050 frames Cell Events: 49 mean_circ: 0.934401
Ran for 1550 frames Cell Events: 74 mean_circ: 0.930571
Ran for 2050 frames Cell Events: 108 mean_circ: 0.92852
Ran for 4550 frames Cell Events: 290 mean_circ: 0.93499
Ran for 5050 frames Cell Events: 320 mean_circ: 0.933994
Ran for 6050 frames Cell Events: 354 mean_circ: 0.934382
Ran for 6550 frames Cell Events: 385 mean_circ: 0.934459
Ran for 7050 frames Cell Events: 414 mean_circ: 0.934853
Ran for 7550 frames Cell Events: 441 mean_circ: 0.934965
Ran for 9550 frames Cell Events: 572 mean_circ: 0.933934
Ran for 10050 frames Cell Events: 602 mean_circ: 0.933752
Ran for 11550 frames Cell Events: 688 mean_circ: 0.933478
Ran for 12050 frames Cell Events: 728 mean_circ: 0.933355
Ran for 13550 frames Cell Events: 820 mean_circ: 0.933136
Ran for 14550 frames Cell Events: 895 mean_circ: 0.933558
Ran for 15050 frames Cell Events: 917 mean_circ: 0.933704
Ran for 15550 frames Cell Events: 945 mean_circ: 0.933864
Ran for 16050 frames Cell Events: 965 mean_circ: 0.933994
Ran for 17050 frames Cell Events: 1021 mean_circ: 0.934217
Ran for 19050 frames Cell Events: 1149 mean_circ: 0.934354
Ran for 19550 frames Cell Events: 1175 mean_circ: 0.934153
Ran for 20550 frames Cell Events: 1222 mean_circ: 0.934307
Ran for 21050 frames Cell Events: 1248 mean_circ: 0.934172
Ran for 21550 frames Cell Events: 1290 mean_circ: 0.93418
Ran for 22050 frames Cell Events: 1320 mean_circ: 0.933864
Ran for 23050 frames Cell Events: 1381 mean_circ: 0.934288
Ran for 24050 frames Cell Events: 1435 mean_circ: 0.934185
Ran for 24550 frames Cell Events: 1460 mean_circ: 0.93417
Ran for 25550 frames Cell Events: 1525 mean_circ: 0.934242
Ran for 26050 frames Cell Events: 1553 mean_circ: 0.934296
Ran for 26550 frames Cell Events: 1574 mean_circ: 0.934228
Ran for 28550 frames Cell Events: 1701 mean_circ: 0.934337
Ran for 29050 frames Cell Events: 1732 mean_circ: 0.934406
Ran for 31050 frames Cell Events: 1869 mean_circ: 0.934444
Ran for 31550 frames Cell Events: 1892 mean_circ: 0.934454
Ran for 32550 frames Cell Events: 1952 mean_circ: 0.934441
Ran for 34050 frames Cell Events: 2042 mean_circ: 0.934648
Ran for 34550 frames Cell Events: 2067 mean_circ: 0.934643
Ran for 35050 frames Cell Events: 2091 mean_circ: 0.934577
Ran for 37050 frames Cell Events: 2230 mean_circ: 0.934851
Ran for 38050 frames Cell Events: 2303 mean_circ: 0.934763
Ran for 38550 frames Cell Events: 2336 mean_circ: 0.934546
Ran for 39050 frames Cell Events: 2360 mean_circ: 0.934631
Ran for 39550 frames Cell Events: 2400 mean_circ: 0.934503
Ran for 40550 frames Cell Events: 2467 mean_circ: 0.93437
Ran for 41050 frames Cell Events: 2490 mean_circ: 0.934401
Ran for 42050 frames Cell Events: 2537 mean_circ: 0.934398
Ran for 42550 frames Cell Events: 2555 mean_circ: 0.934467
Ran for 44050 frames Cell Events: 2645 mean_circ: 0.934438
Ran for 45050 frames Cell Events: 2700 mean_circ: 0.934276
Ran for 46050 frames Cell Events: 2753 mean_circ: 0.934482
Ran for 47050 frames Cell Events: 2822 mean_circ: 0.934684
Ran for 47550 frames Cell Events: 2856 mean_circ: 0.934542
Ran for 48050 frames Cell Events: 2883 mean_circ: 0.934584
Ran for 49050 frames Cell Events: 2955 mean_circ: 0.934707
Ran for 49550 frames Cell Events: 2987 mean_circ: 0.934767
Ran for 50050 frames Cell Events: 3013 mean_circ: 0.934818
Ran for 50550 frames Cell Events: 3037 mean_circ: 0.93491
Ran for 51050 frames Cell Events: 3064 mean_circ: 0.934919
Ran for 51550 frames Cell Events: 3090 mean_circ: 0.934871
Ran for 52050 frames Cell Events: 3115 mean_circ: 0.934777
Ran for 53050 frames Cell Events: 3159 mean_circ: 0.934919
Ran for 54050 frames Cell Events: 3238 mean_circ: 0.934812
Ran for 55050 frames Cell Events: 3295 mean_circ: 0.934694
Ran for 55550 frames Cell Events: 3323 mean_circ: 0.934747
Ran for 56550 frames Cell Events: 3363 mean_circ: 0.934782
Ran for 58050 frames Cell Events: 3448 mean_circ: 0.934861
Ran for 58550 frames Cell Events: 3479 mean_circ: 0.934896
Ran for 59050 frames Cell Events: 3522 mean_circ: 0.935025
Ran for 61050 frames Cell Events: 3621 mean_circ: 0.935147
Ran for 63050 frames Cell Events: 3730 mean_circ: 0.935304
Ran for 63550 frames Cell Events: 3751 mean_circ: 0.93532
Ran for 64050 frames Cell Events: 3782 mean_circ: 0.935204
Ran for 64550 frames Cell Events: 3809 mean_circ: 0.935146
Ran for 65050 frames Cell Events: 3834 mean_circ: 0.935224
Ran for 65550 frames Cell Events: 3851 mean_circ: 0.935227
Ran for 67050 frames Cell Events: 3932 mean_circ: 0.935276
Ran for 67550 frames Cell Events: 3961 mean_circ: 0.935282
Ran for 69550 frames Cell Events: 4086 mean_circ: 0.935098
Ran for 70050 frames Cell Events: 4110 mean_circ: 0.935143
Ran for 70550 frames Cell Events: 4136 mean_circ: 0.935179
Ran for 71050 frames Cell Events: 4154 mean_circ: 0.935116
Ran for 71550 frames Cell Events: 4182 mean_circ: 0.935218
Ran for 72050 frames Cell Events: 4211 mean_circ: 0.935294
Ran for 73550 frames Cell Events: 4325 mean_circ: 0.935026
Ran for 74050 frames Cell Events: 4350 mean_circ: 0.935067
Ran for 74550 frames Cell Events: 4386 mean_circ: 0.935067
Ran for 75550 frames Cell Events: 4434 mean_circ: 0.935086
Ran for 76550 frames Cell Events: 4474 mean_circ: 0.935101
Ran for 77050 frames Cell Events: 4503 mean_circ: 0.935103
Ran for 78050 frames Cell Events: 4569 mean_circ: 0.935095
Ran for 80050 frames Cell Events: 4711 mean_circ: 0.935008
Ran for 82050 frames Cell Events: 4858 mean_circ: 0.934729
Ran for 83050 frames Cell Events: 4916 mean_circ: 0.934727
Ran for 83550 frames Cell Events: 4958 mean_circ: 0.934712
Ran for 84550 frames Cell Events: 5028 mean_circ: 0.934704
Ran for 89050 frames Cell Events: 5312 mean_circ: 0.934776
Ran for 89550 frames Cell Events: 5342 mean_circ: 0.934764
Ran for 91550 frames Cell Events: 5482 mean_circ: 0.934716
Ran for 92050 frames Cell Events: 5511 mean_circ: 0.934771
Ran for 92550 frames Cell Events: 5547 mean_circ: 0.934718
Ran for 93550 frames Cell Events: 5615 mean_circ: 0.934671
Ran for 94050 frames Cell Events: 5647 mean_circ: 0.934732
Ran for 94550 frames Cell Events: 5692 mean_circ: 0.934732
Ran for 95050 frames Cell Events: 5718 mean_circ: 0.934866
Ran for 95550 frames Cell Events: 5745 mean_circ: 0.934869
Ran for 96550 frames Cell Events: 5807 mean_circ: 0.93485
Ran for 97050 frames Cell Events: 5838 mean_circ: 0.934858
Ran for 98050 frames Cell Events: 5909 mean_circ: 0.934822
Ran for 98550 frames Cell Events: 5926 mean_circ: 0.934834
Ran for 99550 frames Cell Events: 5984 mean_circ: 0.934769
Ran for 100050 frames Cell Events: 6014 mean_circ: 0.934763
Ran for 101050 frames Cell Events: 6091 mean_circ: 0.934839
Ran for 101550 frames Cell Events: 6120 mean_circ: 0.934878
Ran for 102550 frames Cell Events: 6182 mean_circ: 0.934869
Ran for 103550 frames Cell Events: 6236 mean_circ: 0.934918
Ran for 104550 frames Cell Events: 6274 mean_circ: 0.934999
Ran for 105050 frames Cell Events: 6287 mean_circ: 0.935026
Ran for 106050 frames Cell Events: 6338 mean_circ: 0.935033
Ran for 106550 frames Cell Events: 6360 mean_circ: 0.935038
Ran for 109050 frames Cell Events: 6495 mean_circ: 0.935012
Ran for 111550 frames Cell Events: 6665 mean_circ: 0.934828
Ran for 112050 frames Cell Events: 6695 mean_circ: 0.934832
Ran for 112550 frames Cell Events: 6722 mean_circ: 0.934745
Ran for 113550 frames Cell Events: 6768 mean_circ: 0.934744
Ran for 114550 frames Cell Events: 6827 mean_circ: 0.934705
Ran for 115050 frames Cell Events: 6852 mean_circ: 0.934717
Ran for 117050 frames Cell Events: 6949 mean_circ: 0.934727
Ran for 118050 frames Cell Events: 7011 mean_circ: 0.934762
Ran for 118550 frames Cell Events: 7035 mean_circ: 0.934785
Ran for 120050 frames Cell Events: 7118 mean_circ: 0.934795
Ran for 120550 frames Cell Events: 7149 mean_circ: 0.934788
Ran for 121550 frames Cell Events: 7211 mean_circ: 0.934839
Ran for 122050 frames Cell Events: 7242 mean_circ: 0.934824
Ran for 123050 frames Cell Events: 7290 mean_circ: 0.934823
Ran for 124550 frames Cell Events: 7391 mean_circ: 0.934812
Ran for 125550 frames Cell Events: 7448 mean_circ: 0.934875
Ran for 126050 frames Cell Events: 7467 mean_circ: 0.934889
Ran for 127050 frames Cell Events: 7518 mean_circ: 0.934901
Ran for 129050 frames Cell Events: 7666 mean_circ: 0.934851
Ran for 129550 frames Cell Events: 7693 mean_circ: 0.934871
Ran for 130050 frames Cell Events: 7732 mean_circ: 0.934919
Ran for 131050 frames Cell Events: 7782 mean_circ: 0.934897
Ran for 131550 frames Cell Events: 7808 mean_circ: 0.934916
Ran for 132050 frames Cell Events: 7833 mean_circ: 0.934882
Ran for 133050 frames Cell Events: 7890 mean_circ: 0.934926
Ran for 133550 frames Cell Events: 7925 mean_circ: 0.934989
Ran for 134050 frames Cell Events: 7946 mean_circ: 0.935025
Ran for 135050 frames Cell Events: 8002 mean_circ: 0.935002
Ran for 135550 frames Cell Events: 8028 mean_circ: 0.934993
Ran for 136550 frames Cell Events: 8079 mean_circ: 0.935048
Ran for 137050 frames Cell Events: 8096 mean_circ: 0.935092
Ran for 137550 frames Cell Events: 8128 mean_circ: 0.935071
Ran for 138550 frames Cell Events: 8183 mean_circ: 0.935072
Ran for 139050 frames Cell Events: 8208 mean_circ: 0.935095
Ran for 139550 frames Cell Events: 8230 mean_circ: 0.935085
Ran for 140050 frames Cell Events: 8261 mean_circ: 0.935031
Ran for 141050 frames Cell Events: 8319 mean_circ: 0.935034
Ran for 141550 frames Cell Events: 8343 mean_circ: 0.935065



End this measurment..



Stopping time: 3.80511e+007
****************************************
Measurement finished

Mean Circularitiy: 0.93504
Mean Area: 645.965
Mean FL-1 intensity: 3704.5
Mean FL-2 intensity: 5446.22
Mean FL-3 intensity: 0

frames processed: 141435
Total Time: 70984.1ms
Number of Runs (new bg img) 0
Frames collected: 141850
Skipped frames: 107511
Rescued Frames: 4917754
Samples DAQ: 35462593
Samples written: 70925064
Samples per Channel: 35462532
FPS: 1998.33

Samples per second: 499584
Expected samples/s: 500000

Events: 8357
Events per second: 117.731
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :217669
# 1 frames :53877
# 2 frames :2874
# 3 frames :953
# 4 frames :557
# 5 frames :237
# 6 frames :1855
# 7 frames :1124
# 8 frames :1007
# 9 frames :598
# 10 frames :787
# 11 frames :410
# 12 frames :251
# 13 frames :147
# 14 frames :216
# 15 frames :194
# 16 frames :172
# 17 frames :115
# 18 frames :92
# 19 frames :67
# 20 frames :61
# 21 frames :49
# 22 frames :52
# 23 frames :43
# 24 frames :55
# 25 frames :32
# 26 frames :31
# 27 frames :20
# 28 frames :16
# 29 frames :24
# 30 frames :12
# 31 frames :11
# 32 frames :9
# 33 frames :6
# 34 frames :6
# 35 frames :3
# 36 frames :5
# 37 frames :12
# 38 frames :4
# 39 frames :3
# 40 frames :1
# 41 frames :4
# 43 frames :4
# 44 frames :1
# 46 frames :3
# 47 frames :3
# 49 frames :1
# 50 frames :2
# 51 frames :1
# 54 frames :2
# 57 frames :1
# 62 frames :1
# 68 frames :1
# 69 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.3398
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0