Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "10924a83cfeaf7b35e67f3e43c602cb6",
"name": "M1_20170515_1918_dsRNA_D2_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:27:00"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8475 (max 8476) in '8475'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.3479
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.93778e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.937614
Ran for 1050 frames Cell Events: 48 mean_circ: 0.931571
Ran for 1550 frames Cell Events: 77 mean_circ: 0.92682
Ran for 2550 frames Cell Events: 137 mean_circ: 0.926345
Ran for 3050 frames Cell Events: 157 mean_circ: 0.929946
Ran for 3550 frames Cell Events: 180 mean_circ: 0.932034
Ran for 6050 frames Cell Events: 292 mean_circ: 0.931544
Ran for 6550 frames Cell Events: 310 mean_circ: 0.931357
Ran for 7050 frames Cell Events: 334 mean_circ: 0.932267
Ran for 7550 frames Cell Events: 364 mean_circ: 0.932086
Ran for 8050 frames Cell Events: 381 mean_circ: 0.931879
Ran for 8550 frames Cell Events: 408 mean_circ: 0.931907
Ran for 10050 frames Cell Events: 483 mean_circ: 0.932183
Ran for 11050 frames Cell Events: 537 mean_circ: 0.932701
Ran for 11550 frames Cell Events: 568 mean_circ: 0.932864
Ran for 13050 frames Cell Events: 672 mean_circ: 0.932743
Ran for 17050 frames Cell Events: 913 mean_circ: 0.931108
Ran for 17550 frames Cell Events: 942 mean_circ: 0.930998
Ran for 18050 frames Cell Events: 969 mean_circ: 0.931085
Ran for 18550 frames Cell Events: 996 mean_circ: 0.931196
Ran for 19050 frames Cell Events: 1019 mean_circ: 0.931412
Ran for 19550 frames Cell Events: 1048 mean_circ: 0.931145
Ran for 20050 frames Cell Events: 1076 mean_circ: 0.93139
Ran for 20550 frames Cell Events: 1110 mean_circ: 0.930762
Ran for 21050 frames Cell Events: 1137 mean_circ: 0.930594
Ran for 21550 frames Cell Events: 1160 mean_circ: 0.930489
Ran for 22050 frames Cell Events: 1189 mean_circ: 0.930558
Ran for 24050 frames Cell Events: 1309 mean_circ: 0.930591
Ran for 24550 frames Cell Events: 1335 mean_circ: 0.930795
Ran for 25550 frames Cell Events: 1394 mean_circ: 0.930023
Ran for 26050 frames Cell Events: 1422 mean_circ: 0.930215
Ran for 27050 frames Cell Events: 1479 mean_circ: 0.93006
Ran for 28050 frames Cell Events: 1542 mean_circ: 0.930296
Ran for 28550 frames Cell Events: 1569 mean_circ: 0.930313
Ran for 29050 frames Cell Events: 1601 mean_circ: 0.930341
Ran for 30050 frames Cell Events: 1657 mean_circ: 0.930731
Ran for 30550 frames Cell Events: 1681 mean_circ: 0.930299
Ran for 31050 frames Cell Events: 1700 mean_circ: 0.930156
Ran for 32550 frames Cell Events: 1792 mean_circ: 0.930205
Ran for 33050 frames Cell Events: 1821 mean_circ: 0.930155
Ran for 34050 frames Cell Events: 1876 mean_circ: 0.930449
Ran for 35050 frames Cell Events: 1944 mean_circ: 0.930389
Ran for 35550 frames Cell Events: 1966 mean_circ: 0.930429
Ran for 36050 frames Cell Events: 1996 mean_circ: 0.930502
Ran for 36550 frames Cell Events: 2028 mean_circ: 0.930106
Ran for 37550 frames Cell Events: 2114 mean_circ: 0.930157
Ran for 38050 frames Cell Events: 2143 mean_circ: 0.930044
Ran for 39050 frames Cell Events: 2196 mean_circ: 0.930121
Ran for 40050 frames Cell Events: 2286 mean_circ: 0.930212
Ran for 41050 frames Cell Events: 2368 mean_circ: 0.930236
Ran for 41550 frames Cell Events: 2396 mean_circ: 0.930354
Ran for 42550 frames Cell Events: 2474 mean_circ: 0.93057
Ran for 43550 frames Cell Events: 2526 mean_circ: 0.930843
Ran for 44550 frames Cell Events: 2592 mean_circ: 0.930767
Ran for 45050 frames Cell Events: 2614 mean_circ: 0.930901
Ran for 46550 frames Cell Events: 2709 mean_circ: 0.930925
Ran for 47550 frames Cell Events: 2756 mean_circ: 0.930875
Ran for 48050 frames Cell Events: 2788 mean_circ: 0.930884
Ran for 48550 frames Cell Events: 2805 mean_circ: 0.930922
Ran for 49050 frames Cell Events: 2832 mean_circ: 0.931031
Ran for 49550 frames Cell Events: 2860 mean_circ: 0.930975
Ran for 50050 frames Cell Events: 2889 mean_circ: 0.930953
Ran for 50550 frames Cell Events: 2913 mean_circ: 0.930842
Ran for 53050 frames Cell Events: 3072 mean_circ: 0.930692
Ran for 53550 frames Cell Events: 3091 mean_circ: 0.930652
Ran for 55050 frames Cell Events: 3171 mean_circ: 0.930664
Ran for 56050 frames Cell Events: 3236 mean_circ: 0.930776
Ran for 57550 frames Cell Events: 3322 mean_circ: 0.931023
Ran for 59050 frames Cell Events: 3412 mean_circ: 0.930912
Ran for 60050 frames Cell Events: 3460 mean_circ: 0.930838
Ran for 60550 frames Cell Events: 3495 mean_circ: 0.930922
Ran for 61550 frames Cell Events: 3573 mean_circ: 0.930637
Ran for 62050 frames Cell Events: 3593 mean_circ: 0.930717
Ran for 63050 frames Cell Events: 3646 mean_circ: 0.930878
Ran for 63550 frames Cell Events: 3668 mean_circ: 0.93098
Ran for 65050 frames Cell Events: 3750 mean_circ: 0.931082
Ran for 69550 frames Cell Events: 4073 mean_circ: 0.930723
Ran for 70050 frames Cell Events: 4100 mean_circ: 0.930695
Ran for 71550 frames Cell Events: 4194 mean_circ: 0.930815
Ran for 72050 frames Cell Events: 4226 mean_circ: 0.930877
Ran for 73050 frames Cell Events: 4284 mean_circ: 0.931006
Ran for 73550 frames Cell Events: 4301 mean_circ: 0.931026
Ran for 74050 frames Cell Events: 4330 mean_circ: 0.930954
Ran for 75050 frames Cell Events: 4380 mean_circ: 0.930785
Ran for 78050 frames Cell Events: 4582 mean_circ: 0.930671
Ran for 79050 frames Cell Events: 4643 mean_circ: 0.93061
Ran for 79550 frames Cell Events: 4664 mean_circ: 0.930705
Ran for 81050 frames Cell Events: 4761 mean_circ: 0.930713
Ran for 81550 frames Cell Events: 4791 mean_circ: 0.930725
Ran for 82050 frames Cell Events: 4819 mean_circ: 0.93073
Ran for 82550 frames Cell Events: 4843 mean_circ: 0.930717
Ran for 83550 frames Cell Events: 4897 mean_circ: 0.930761
Ran for 84050 frames Cell Events: 4918 mean_circ: 0.930777
Ran for 85550 frames Cell Events: 5014 mean_circ: 0.930773
Ran for 86050 frames Cell Events: 5044 mean_circ: 0.930731
Ran for 86550 frames Cell Events: 5075 mean_circ: 0.930691
Ran for 88050 frames Cell Events: 5173 mean_circ: 0.930626
Ran for 90550 frames Cell Events: 5384 mean_circ: 0.930497
Ran for 92050 frames Cell Events: 5484 mean_circ: 0.930491
Ran for 93550 frames Cell Events: 5550 mean_circ: 0.9305
Ran for 96050 frames Cell Events: 5699 mean_circ: 0.930487
Ran for 97550 frames Cell Events: 5791 mean_circ: 0.930536
Ran for 98050 frames Cell Events: 5817 mean_circ: 0.930553
Ran for 98550 frames Cell Events: 5830 mean_circ: 0.930563
Ran for 101550 frames Cell Events: 6015 mean_circ: 0.930379
Ran for 102050 frames Cell Events: 6039 mean_circ: 0.930397
Ran for 102550 frames Cell Events: 6065 mean_circ: 0.930395
Ran for 103550 frames Cell Events: 6124 mean_circ: 0.930307
Ran for 104050 frames Cell Events: 6142 mean_circ: 0.930251
Ran for 104550 frames Cell Events: 6160 mean_circ: 0.930213
Ran for 106050 frames Cell Events: 6259 mean_circ: 0.9303
Ran for 108050 frames Cell Events: 6405 mean_circ: 0.930394
Ran for 110050 frames Cell Events: 6544 mean_circ: 0.93037
Ran for 110550 frames Cell Events: 6565 mean_circ: 0.930413
Ran for 111550 frames Cell Events: 6621 mean_circ: 0.930422
Ran for 113550 frames Cell Events: 6759 mean_circ: 0.930535
Ran for 115050 frames Cell Events: 6834 mean_circ: 0.930541
Ran for 116550 frames Cell Events: 6915 mean_circ: 0.930456
Ran for 117550 frames Cell Events: 6978 mean_circ: 0.930521
Ran for 119050 frames Cell Events: 7065 mean_circ: 0.930436
Ran for 120050 frames Cell Events: 7121 mean_circ: 0.930402
Ran for 120550 frames Cell Events: 7147 mean_circ: 0.930383
Ran for 122550 frames Cell Events: 7259 mean_circ: 0.930369
Ran for 123050 frames Cell Events: 7285 mean_circ: 0.930362
Ran for 125050 frames Cell Events: 7425 mean_circ: 0.930333
Ran for 126050 frames Cell Events: 7464 mean_circ: 0.930344
Ran for 126550 frames Cell Events: 7492 mean_circ: 0.930373
Ran for 127050 frames Cell Events: 7510 mean_circ: 0.930407
Ran for 127550 frames Cell Events: 7543 mean_circ: 0.930421
Ran for 128050 frames Cell Events: 7583 mean_circ: 0.930313
Ran for 128550 frames Cell Events: 7606 mean_circ: 0.930357
Ran for 130550 frames Cell Events: 7721 mean_circ: 0.930244
Ran for 132050 frames Cell Events: 7806 mean_circ: 0.930302
Ran for 132550 frames Cell Events: 7838 mean_circ: 0.930245
Ran for 133050 frames Cell Events: 7864 mean_circ: 0.930249
Ran for 133550 frames Cell Events: 7886 mean_circ: 0.930124
Ran for 134550 frames Cell Events: 7942 mean_circ: 0.930174
Ran for 135550 frames Cell Events: 8018 mean_circ: 0.930082
Ran for 136050 frames Cell Events: 8043 mean_circ: 0.930037
Ran for 136550 frames Cell Events: 8061 mean_circ: 0.930014
Ran for 137050 frames Cell Events: 8086 mean_circ: 0.929976
Ran for 139050 frames Cell Events: 8166 mean_circ: 0.929861
Ran for 140550 frames Cell Events: 8272 mean_circ: 0.929885
Ran for 142550 frames Cell Events: 8385 mean_circ: 0.929851



End this measurment..



Stopping time: 3.94497e+007
****************************************
Measurement finished

Mean Circularitiy: 0.929777
Mean Area: 684.961
Mean FL-1 intensity: 3451.74
Mean FL-2 intensity: 5187.47
Mean FL-3 intensity: 0

frames processed: 141567
Total Time: 71968.9ms
Number of Runs (new bg img) 0
Frames collected: 143795
Skipped frames: 118093
Rescued Frames: 5245433
Samples DAQ: 35950448
Samples written: 71897636
Samples per Channel: 35948818
FPS: 1998.02

Samples per second: 499505
Expected samples/s: 500000

Events: 8476
Events per second: 117.773
jumped back (PMT): 23
#Frames read, frequency
# 0 frames :219210
# 1 frames :56591
# 2 frames :2576
# 3 frames :927
# 4 frames :425
# 5 frames :203
# 6 frames :2521
# 7 frames :1163
# 8 frames :1020
# 9 frames :533
# 10 frames :356
# 11 frames :225
# 12 frames :213
# 13 frames :217
# 14 frames :233
# 15 frames :200
# 16 frames :127
# 17 frames :98
# 18 frames :84
# 19 frames :66
# 20 frames :70
# 21 frames :51
# 22 frames :68
# 23 frames :50
# 24 frames :39
# 25 frames :21
# 26 frames :27
# 27 frames :31
# 28 frames :13
# 29 frames :15
# 30 frames :10
# 31 frames :19
# 32 frames :9
# 33 frames :14
# 34 frames :11
# 35 frames :10
# 36 frames :13
# 37 frames :8
# 38 frames :8
# 39 frames :4
# 40 frames :2
# 41 frames :5
# 42 frames :4
# 43 frames :5
# 44 frames :1
# 45 frames :2
# 46 frames :6
# 47 frames :5
# 50 frames :3
# 53 frames :1
# 54 frames :4
# 55 frames :1
# 56 frames :1
# 57 frames :2
# 59 frames :1
# 63 frames :1
# 64 frames :1
# 68 frames :1
# 70 frames :1
# 74 frames :1
# 77 frames :1
# 81 frames :1
# 85 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.3479
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0