Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bafb29e116b7d8a41cb4f1980ecd49db",
"name": "M1_20170516_1329_dsRNA_D4_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:29:20"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.1966
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.0484e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.945676
Ran for 550 frames Cell Events: 21 mean_circ: 0.93676
Ran for 1050 frames Cell Events: 42 mean_circ: 0.939482
Ran for 3050 frames Cell Events: 156 mean_circ: 0.939769
Ran for 3550 frames Cell Events: 192 mean_circ: 0.94013
Ran for 4050 frames Cell Events: 229 mean_circ: 0.940497
Ran for 4550 frames Cell Events: 252 mean_circ: 0.940823
Ran for 5550 frames Cell Events: 307 mean_circ: 0.941091
Ran for 6050 frames Cell Events: 326 mean_circ: 0.941617
Ran for 7550 frames Cell Events: 393 mean_circ: 0.941417
Ran for 8050 frames Cell Events: 419 mean_circ: 0.939532
Ran for 8550 frames Cell Events: 450 mean_circ: 0.939082
Ran for 11050 frames Cell Events: 572 mean_circ: 0.937624
Ran for 12550 frames Cell Events: 650 mean_circ: 0.937576
Ran for 13050 frames Cell Events: 666 mean_circ: 0.937432
Ran for 13550 frames Cell Events: 691 mean_circ: 0.937612
Ran for 14050 frames Cell Events: 723 mean_circ: 0.936807
Ran for 15050 frames Cell Events: 774 mean_circ: 0.936436
Ran for 15550 frames Cell Events: 806 mean_circ: 0.936503
Ran for 16050 frames Cell Events: 829 mean_circ: 0.936591
Ran for 17550 frames Cell Events: 898 mean_circ: 0.936161
Ran for 18050 frames Cell Events: 913 mean_circ: 0.936169
Ran for 18550 frames Cell Events: 928 mean_circ: 0.936243
Ran for 20050 frames Cell Events: 1005 mean_circ: 0.93614
Ran for 21050 frames Cell Events: 1059 mean_circ: 0.93567
Ran for 21550 frames Cell Events: 1079 mean_circ: 0.935677
Ran for 23550 frames Cell Events: 1164 mean_circ: 0.935804
Ran for 24050 frames Cell Events: 1187 mean_circ: 0.935963
Ran for 24550 frames Cell Events: 1218 mean_circ: 0.935725
Ran for 25050 frames Cell Events: 1240 mean_circ: 0.935445
Ran for 26050 frames Cell Events: 1288 mean_circ: 0.935444
Ran for 27050 frames Cell Events: 1331 mean_circ: 0.935681
Ran for 27550 frames Cell Events: 1355 mean_circ: 0.935624
Ran for 28550 frames Cell Events: 1406 mean_circ: 0.935638
Ran for 29050 frames Cell Events: 1425 mean_circ: 0.935549
Ran for 30050 frames Cell Events: 1484 mean_circ: 0.93593
Ran for 30550 frames Cell Events: 1514 mean_circ: 0.935927
Ran for 32050 frames Cell Events: 1632 mean_circ: 0.936088
Ran for 32550 frames Cell Events: 1651 mean_circ: 0.936061
Ran for 33050 frames Cell Events: 1668 mean_circ: 0.936073
Ran for 35550 frames Cell Events: 1792 mean_circ: 0.935766
Ran for 37050 frames Cell Events: 1859 mean_circ: 0.935814
Ran for 37550 frames Cell Events: 1870 mean_circ: 0.935858
Ran for 38550 frames Cell Events: 1902 mean_circ: 0.936008
Ran for 39050 frames Cell Events: 1923 mean_circ: 0.936057
Ran for 40550 frames Cell Events: 1991 mean_circ: 0.936317
Ran for 41050 frames Cell Events: 2001 mean_circ: 0.936356
Ran for 41550 frames Cell Events: 2016 mean_circ: 0.936386
Ran for 42050 frames Cell Events: 2038 mean_circ: 0.93642
Ran for 44550 frames Cell Events: 2154 mean_circ: 0.93652
Ran for 45050 frames Cell Events: 2176 mean_circ: 0.936566
Ran for 45550 frames Cell Events: 2200 mean_circ: 0.936553
Ran for 46050 frames Cell Events: 2233 mean_circ: 0.936459
Ran for 46550 frames Cell Events: 2252 mean_circ: 0.936448
Ran for 48550 frames Cell Events: 2370 mean_circ: 0.936422
Ran for 50050 frames Cell Events: 2434 mean_circ: 0.936315
Ran for 50550 frames Cell Events: 2461 mean_circ: 0.936307
Ran for 51550 frames Cell Events: 2505 mean_circ: 0.936204
Ran for 52050 frames Cell Events: 2534 mean_circ: 0.936315
Ran for 52550 frames Cell Events: 2554 mean_circ: 0.936407
Ran for 53050 frames Cell Events: 2578 mean_circ: 0.936371
Ran for 53550 frames Cell Events: 2604 mean_circ: 0.936254
Ran for 56550 frames Cell Events: 2747 mean_circ: 0.936013
Ran for 57050 frames Cell Events: 2766 mean_circ: 0.936069
Ran for 57550 frames Cell Events: 2782 mean_circ: 0.936147
Ran for 60050 frames Cell Events: 2894 mean_circ: 0.93615
Ran for 60550 frames Cell Events: 2917 mean_circ: 0.936117
Ran for 61550 frames Cell Events: 2965 mean_circ: 0.936125
Ran for 62050 frames Cell Events: 2983 mean_circ: 0.936203
Ran for 62550 frames Cell Events: 2998 mean_circ: 0.936165
Ran for 64550 frames Cell Events: 3087 mean_circ: 0.936311
Ran for 65550 frames Cell Events: 3125 mean_circ: 0.936329
Ran for 66050 frames Cell Events: 3153 mean_circ: 0.93646
Ran for 67550 frames Cell Events: 3222 mean_circ: 0.936528
Ran for 68050 frames Cell Events: 3251 mean_circ: 0.93656
Ran for 68550 frames Cell Events: 3267 mean_circ: 0.936518
Ran for 69050 frames Cell Events: 3288 mean_circ: 0.936504
Ran for 69550 frames Cell Events: 3306 mean_circ: 0.936527
Ran for 70050 frames Cell Events: 3322 mean_circ: 0.936569
Ran for 70550 frames Cell Events: 3343 mean_circ: 0.93655
Ran for 71050 frames Cell Events: 3375 mean_circ: 0.936569
Ran for 71550 frames Cell Events: 3399 mean_circ: 0.936543
Ran for 72550 frames Cell Events: 3453 mean_circ: 0.936441
Ran for 73550 frames Cell Events: 3497 mean_circ: 0.936583
Ran for 74050 frames Cell Events: 3520 mean_circ: 0.936559
Ran for 75050 frames Cell Events: 3584 mean_circ: 0.936569
Ran for 75550 frames Cell Events: 3608 mean_circ: 0.936564
Ran for 77050 frames Cell Events: 3678 mean_circ: 0.936595
Ran for 77550 frames Cell Events: 3711 mean_circ: 0.936555
Ran for 78050 frames Cell Events: 3732 mean_circ: 0.936543
Ran for 79050 frames Cell Events: 3777 mean_circ: 0.936511
Ran for 80550 frames Cell Events: 3861 mean_circ: 0.93653
Ran for 81050 frames Cell Events: 3882 mean_circ: 0.936517
Ran for 82550 frames Cell Events: 3972 mean_circ: 0.936517
Ran for 83050 frames Cell Events: 3987 mean_circ: 0.936537
Ran for 83550 frames Cell Events: 4008 mean_circ: 0.936587
Ran for 84050 frames Cell Events: 4030 mean_circ: 0.936549
Ran for 84550 frames Cell Events: 4055 mean_circ: 0.936637
Ran for 85050 frames Cell Events: 4089 mean_circ: 0.936646
Ran for 86050 frames Cell Events: 4144 mean_circ: 0.936568
Ran for 87050 frames Cell Events: 4193 mean_circ: 0.936612
Ran for 87550 frames Cell Events: 4225 mean_circ: 0.936587
Ran for 88050 frames Cell Events: 4247 mean_circ: 0.936647
Ran for 88550 frames Cell Events: 4276 mean_circ: 0.936612
Ran for 89050 frames Cell Events: 4300 mean_circ: 0.936577
Ran for 89550 frames Cell Events: 4316 mean_circ: 0.936606
Ran for 90050 frames Cell Events: 4336 mean_circ: 0.936663
Ran for 90550 frames Cell Events: 4365 mean_circ: 0.936663
Ran for 91550 frames Cell Events: 4426 mean_circ: 0.936723
Ran for 93050 frames Cell Events: 4522 mean_circ: 0.936786
Ran for 93550 frames Cell Events: 4540 mean_circ: 0.936828
Ran for 95050 frames Cell Events: 4614 mean_circ: 0.936932
Ran for 95550 frames Cell Events: 4635 mean_circ: 0.936914
Ran for 96550 frames Cell Events: 4689 mean_circ: 0.936964
Ran for 97050 frames Cell Events: 4715 mean_circ: 0.936964
Ran for 98550 frames Cell Events: 4781 mean_circ: 0.937003
Ran for 99050 frames Cell Events: 4800 mean_circ: 0.936987
Ran for 101050 frames Cell Events: 4879 mean_circ: 0.937037
Ran for 103050 frames Cell Events: 4988 mean_circ: 0.937102
Ran for 103550 frames Cell Events: 5003 mean_circ: 0.937119
Ran for 104050 frames Cell Events: 5022 mean_circ: 0.93706
Ran for 104550 frames Cell Events: 5045 mean_circ: 0.937064
Ran for 105550 frames Cell Events: 5102 mean_circ: 0.937075
Ran for 106050 frames Cell Events: 5127 mean_circ: 0.937006
Ran for 108050 frames Cell Events: 5244 mean_circ: 0.937131
Ran for 108550 frames Cell Events: 5262 mean_circ: 0.937088
Ran for 109550 frames Cell Events: 5309 mean_circ: 0.937055
Ran for 110050 frames Cell Events: 5334 mean_circ: 0.937048
Ran for 111050 frames Cell Events: 5376 mean_circ: 0.937011
Ran for 111550 frames Cell Events: 5397 mean_circ: 0.93697
Ran for 113550 frames Cell Events: 5489 mean_circ: 0.93686
Ran for 116050 frames Cell Events: 5629 mean_circ: 0.936813
Ran for 116550 frames Cell Events: 5652 mean_circ: 0.936788
Ran for 119050 frames Cell Events: 5766 mean_circ: 0.936853
Ran for 119550 frames Cell Events: 5789 mean_circ: 0.936836
Ran for 120550 frames Cell Events: 5853 mean_circ: 0.936852
Ran for 121050 frames Cell Events: 5870 mean_circ: 0.936852
Ran for 122050 frames Cell Events: 5914 mean_circ: 0.936888
Ran for 122550 frames Cell Events: 5938 mean_circ: 0.936889
Ran for 124050 frames Cell Events: 6010 mean_circ: 0.936936
Ran for 126050 frames Cell Events: 6106 mean_circ: 0.93687
Ran for 126550 frames Cell Events: 6138 mean_circ: 0.936892
Ran for 127050 frames Cell Events: 6162 mean_circ: 0.93689
Ran for 127550 frames Cell Events: 6187 mean_circ: 0.936901
Ran for 128050 frames Cell Events: 6214 mean_circ: 0.936906
Ran for 128550 frames Cell Events: 6239 mean_circ: 0.936914
Ran for 129550 frames Cell Events: 6295 mean_circ: 0.93691
Ran for 130050 frames Cell Events: 6311 mean_circ: 0.936917
Ran for 130550 frames Cell Events: 6332 mean_circ: 0.936883
Ran for 131050 frames Cell Events: 6355 mean_circ: 0.936892
Ran for 132050 frames Cell Events: 6397 mean_circ: 0.936804
Ran for 133550 frames Cell Events: 6476 mean_circ: 0.936783
Ran for 134050 frames Cell Events: 6498 mean_circ: 0.936754
Ran for 135050 frames Cell Events: 6568 mean_circ: 0.936762
Ran for 136050 frames Cell Events: 6618 mean_circ: 0.93674
Ran for 136550 frames Cell Events: 6637 mean_circ: 0.936738
Ran for 137050 frames Cell Events: 6655 mean_circ: 0.936715
Ran for 137550 frames Cell Events: 6668 mean_circ: 0.936713
Ran for 139050 frames Cell Events: 6732 mean_circ: 0.936696
Ran for 139550 frames Cell Events: 6757 mean_circ: 0.936717
Ran for 140050 frames Cell Events: 6782 mean_circ: 0.936753
Ran for 140550 frames Cell Events: 6791 mean_circ: 0.936756
Ran for 141050 frames Cell Events: 6810 mean_circ: 0.936779
Ran for 141550 frames Cell Events: 6830 mean_circ: 0.936758
Ran for 142550 frames Cell Events: 6871 mean_circ: 0.936775
Ran for 144050 frames Cell Events: 6948 mean_circ: 0.936734
Ran for 145050 frames Cell Events: 6992 mean_circ: 0.936702
Ran for 145550 frames Cell Events: 7013 mean_circ: 0.936735
Ran for 146050 frames Cell Events: 7025 mean_circ: 0.936708
Ran for 146550 frames Cell Events: 7049 mean_circ: 0.936719
Ran for 147550 frames Cell Events: 7103 mean_circ: 0.936717
Ran for 148550 frames Cell Events: 7162 mean_circ: 0.93669
Ran for 149550 frames Cell Events: 7228 mean_circ: 0.936653
Ran for 150550 frames Cell Events: 7280 mean_circ: 0.93662
Ran for 152050 frames Cell Events: 7347 mean_circ: 0.93659
Ran for 152550 frames Cell Events: 7378 mean_circ: 0.936543
Ran for 153550 frames Cell Events: 7424 mean_circ: 0.936561
Ran for 156050 frames Cell Events: 7540 mean_circ: 0.936563
Ran for 156550 frames Cell Events: 7563 mean_circ: 0.936565
Ran for 157550 frames Cell Events: 7601 mean_circ: 0.936577
Ran for 158550 frames Cell Events: 7677 mean_circ: 0.936534
Ran for 159550 frames Cell Events: 7720 mean_circ: 0.936516
Ran for 161050 frames Cell Events: 7817 mean_circ: 0.936453
Ran for 163050 frames Cell Events: 7949 mean_circ: 0.936452
Ran for 163550 frames Cell Events: 7972 mean_circ: 0.936524
Ran for 165550 frames Cell Events: 8071 mean_circ: 0.936532



End this measurment..



Stopping time: 1.04923e+008
****************************************
Measurement finished

Mean Circularitiy: 0.936455
Mean Area: 596.051
Mean FL-1 intensity: 3364.81
Mean FL-2 intensity: 4666.07
Mean FL-3 intensity: 0

frames processed: 165879
Total Time: 83414.4ms
Number of Runs (new bg img) 0
Frames collected: 166710
Skipped frames: 8093
Rescued Frames: 539524
Samples DAQ: 41677929
Samples written: 83355152
Samples per Channel: 41677576
FPS: 1998.58

Samples per second: 499645
Expected samples/s: 500000

Events: 8142
Events per second: 97.6091
jumped back (PMT): 19
#Frames read, frequency
# 0 frames :248714
# 1 frames :70981
# 2 frames :3509
# 3 frames :1215
# 4 frames :632
# 5 frames :317
# 6 frames :1778
# 7 frames :1329
# 8 frames :1019
# 9 frames :622
# 10 frames :668
# 11 frames :690
# 12 frames :361
# 13 frames :203
# 14 frames :217
# 15 frames :206
# 16 frames :163
# 17 frames :141
# 18 frames :83
# 19 frames :74
# 20 frames :54
# 21 frames :68
# 22 frames :44
# 23 frames :57
# 24 frames :29
# 25 frames :20
# 26 frames :22
# 27 frames :18
# 28 frames :14
# 29 frames :13
# 30 frames :16
# 31 frames :9
# 32 frames :7
# 33 frames :7
# 34 frames :6
# 35 frames :5
# 36 frames :4
# 37 frames :4
# 38 frames :3
# 39 frames :6
# 40 frames :3
# 41 frames :2
# 42 frames :5
# 43 frames :7
# 44 frames :3
# 46 frames :1
# 47 frames :2
# 49 frames :1
# 50 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :1
# 55 frames :1
# 56 frames :1
# 57 frames :1
# 58 frames :2
# 70 frames :1
# 74 frames :1
# 76 frames :1
# 78 frames :1
# 90 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.1966
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0