Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8687a0e10d2c2c5b064d69486a033864",
"name": "M1_20170516_1417_dsRNA_E2_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:32:25"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8094 (max 8095) in '8094'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.5429
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.07678e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.93163
Ran for 2050 frames Cell Events: 102 mean_circ: 0.930379
Ran for 2550 frames Cell Events: 135 mean_circ: 0.929217
Ran for 3050 frames Cell Events: 163 mean_circ: 0.930441
Ran for 4050 frames Cell Events: 234 mean_circ: 0.932464
Ran for 5050 frames Cell Events: 285 mean_circ: 0.929912
Ran for 5550 frames Cell Events: 318 mean_circ: 0.931056
Ran for 7050 frames Cell Events: 409 mean_circ: 0.930795
Ran for 7550 frames Cell Events: 435 mean_circ: 0.929994
Ran for 8050 frames Cell Events: 452 mean_circ: 0.929737
Ran for 8550 frames Cell Events: 478 mean_circ: 0.929463
Ran for 9050 frames Cell Events: 495 mean_circ: 0.929586
Ran for 9550 frames Cell Events: 515 mean_circ: 0.929946
Ran for 10050 frames Cell Events: 533 mean_circ: 0.929658
Ran for 10550 frames Cell Events: 551 mean_circ: 0.928846
Ran for 11050 frames Cell Events: 583 mean_circ: 0.928742
Ran for 12050 frames Cell Events: 638 mean_circ: 0.928132
Ran for 13050 frames Cell Events: 705 mean_circ: 0.928593
Ran for 13550 frames Cell Events: 740 mean_circ: 0.928764
Ran for 14050 frames Cell Events: 760 mean_circ: 0.928606
Ran for 14550 frames Cell Events: 783 mean_circ: 0.928694
Ran for 15050 frames Cell Events: 809 mean_circ: 0.928993
Ran for 16050 frames Cell Events: 851 mean_circ: 0.929742
Ran for 16550 frames Cell Events: 879 mean_circ: 0.929747
Ran for 17050 frames Cell Events: 898 mean_circ: 0.929947
Ran for 17550 frames Cell Events: 923 mean_circ: 0.929944
Ran for 18550 frames Cell Events: 992 mean_circ: 0.930947
Ran for 19050 frames Cell Events: 1025 mean_circ: 0.930911
Ran for 19550 frames Cell Events: 1054 mean_circ: 0.930614
Ran for 22050 frames Cell Events: 1206 mean_circ: 0.930725
Ran for 23050 frames Cell Events: 1264 mean_circ: 0.931472
Ran for 26550 frames Cell Events: 1481 mean_circ: 0.931115
Ran for 27050 frames Cell Events: 1512 mean_circ: 0.931529
Ran for 29050 frames Cell Events: 1630 mean_circ: 0.931391
Ran for 30050 frames Cell Events: 1706 mean_circ: 0.931446
Ran for 31050 frames Cell Events: 1765 mean_circ: 0.931487
Ran for 31550 frames Cell Events: 1794 mean_circ: 0.931585
Ran for 32550 frames Cell Events: 1857 mean_circ: 0.93173
Ran for 33550 frames Cell Events: 1909 mean_circ: 0.931751
Ran for 34050 frames Cell Events: 1929 mean_circ: 0.931792
Ran for 34550 frames Cell Events: 1964 mean_circ: 0.931893
Ran for 36050 frames Cell Events: 2057 mean_circ: 0.93193
Ran for 36550 frames Cell Events: 2086 mean_circ: 0.931935
Ran for 37050 frames Cell Events: 2110 mean_circ: 0.931954
Ran for 38050 frames Cell Events: 2168 mean_circ: 0.931675
Ran for 38550 frames Cell Events: 2188 mean_circ: 0.931747
Ran for 39050 frames Cell Events: 2212 mean_circ: 0.931896
Ran for 39550 frames Cell Events: 2232 mean_circ: 0.931789
Ran for 41050 frames Cell Events: 2289 mean_circ: 0.931782
Ran for 41550 frames Cell Events: 2326 mean_circ: 0.931737
Ran for 42050 frames Cell Events: 2355 mean_circ: 0.931567
Ran for 43050 frames Cell Events: 2406 mean_circ: 0.931551
Ran for 43550 frames Cell Events: 2429 mean_circ: 0.931681
Ran for 44050 frames Cell Events: 2459 mean_circ: 0.931567
Ran for 44550 frames Cell Events: 2488 mean_circ: 0.931723
Ran for 45050 frames Cell Events: 2519 mean_circ: 0.931679
Ran for 45550 frames Cell Events: 2556 mean_circ: 0.931292
Ran for 46550 frames Cell Events: 2597 mean_circ: 0.931178
Ran for 48050 frames Cell Events: 2706 mean_circ: 0.931363
Ran for 49050 frames Cell Events: 2743 mean_circ: 0.931425
Ran for 49550 frames Cell Events: 2765 mean_circ: 0.931216
Ran for 50550 frames Cell Events: 2812 mean_circ: 0.931098
Ran for 51050 frames Cell Events: 2836 mean_circ: 0.931101
Ran for 52050 frames Cell Events: 2888 mean_circ: 0.931123
Ran for 52550 frames Cell Events: 2914 mean_circ: 0.931246
Ran for 53050 frames Cell Events: 2947 mean_circ: 0.931138
Ran for 54050 frames Cell Events: 3000 mean_circ: 0.931165
Ran for 56050 frames Cell Events: 3061 mean_circ: 0.931171
Ran for 56550 frames Cell Events: 3085 mean_circ: 0.931203
Ran for 57050 frames Cell Events: 3116 mean_circ: 0.931124
Ran for 57550 frames Cell Events: 3135 mean_circ: 0.931116
Ran for 58550 frames Cell Events: 3179 mean_circ: 0.931093
Ran for 59550 frames Cell Events: 3224 mean_circ: 0.93098
Ran for 60050 frames Cell Events: 3244 mean_circ: 0.930906
Ran for 60550 frames Cell Events: 3260 mean_circ: 0.930777
Ran for 62050 frames Cell Events: 3359 mean_circ: 0.930969
Ran for 63050 frames Cell Events: 3422 mean_circ: 0.930816
Ran for 63550 frames Cell Events: 3446 mean_circ: 0.930854
Ran for 64050 frames Cell Events: 3470 mean_circ: 0.930885
Ran for 64550 frames Cell Events: 3499 mean_circ: 0.931031
Ran for 66050 frames Cell Events: 3558 mean_circ: 0.930989
Ran for 66550 frames Cell Events: 3582 mean_circ: 0.931069
Ran for 68550 frames Cell Events: 3691 mean_circ: 0.931284
Ran for 69050 frames Cell Events: 3735 mean_circ: 0.931393
Ran for 70050 frames Cell Events: 3796 mean_circ: 0.931354
Ran for 70550 frames Cell Events: 3817 mean_circ: 0.931341
Ran for 71050 frames Cell Events: 3843 mean_circ: 0.931315
Ran for 72050 frames Cell Events: 3892 mean_circ: 0.931396
Ran for 72550 frames Cell Events: 3922 mean_circ: 0.93131
Ran for 73050 frames Cell Events: 3941 mean_circ: 0.931313
Ran for 74550 frames Cell Events: 4038 mean_circ: 0.931333
Ran for 75050 frames Cell Events: 4055 mean_circ: 0.931294
Ran for 76050 frames Cell Events: 4119 mean_circ: 0.931231
Ran for 76550 frames Cell Events: 4149 mean_circ: 0.931231
Ran for 77050 frames Cell Events: 4170 mean_circ: 0.931056
Ran for 77550 frames Cell Events: 4202 mean_circ: 0.931084
Ran for 78050 frames Cell Events: 4229 mean_circ: 0.93103
Ran for 80550 frames Cell Events: 4365 mean_circ: 0.931017
Ran for 81550 frames Cell Events: 4415 mean_circ: 0.931
Ran for 82050 frames Cell Events: 4432 mean_circ: 0.931033
Ran for 82550 frames Cell Events: 4454 mean_circ: 0.93112
Ran for 83050 frames Cell Events: 4474 mean_circ: 0.931143
Ran for 83550 frames Cell Events: 4493 mean_circ: 0.931164
Ran for 84050 frames Cell Events: 4515 mean_circ: 0.93115
Ran for 84550 frames Cell Events: 4552 mean_circ: 0.931135
Ran for 85050 frames Cell Events: 4578 mean_circ: 0.931057
Ran for 85550 frames Cell Events: 4594 mean_circ: 0.931107
Ran for 86050 frames Cell Events: 4614 mean_circ: 0.931085
Ran for 87550 frames Cell Events: 4706 mean_circ: 0.931201
Ran for 88550 frames Cell Events: 4755 mean_circ: 0.931103
Ran for 90050 frames Cell Events: 4846 mean_circ: 0.931069
Ran for 91050 frames Cell Events: 4923 mean_circ: 0.931139
Ran for 91550 frames Cell Events: 4947 mean_circ: 0.931155
Ran for 92050 frames Cell Events: 4977 mean_circ: 0.931135
Ran for 92550 frames Cell Events: 4996 mean_circ: 0.931156
Ran for 93050 frames Cell Events: 5015 mean_circ: 0.931132
Ran for 93550 frames Cell Events: 5049 mean_circ: 0.931056
Ran for 94050 frames Cell Events: 5098 mean_circ: 0.931056
Ran for 94550 frames Cell Events: 5136 mean_circ: 0.931056
Ran for 95050 frames Cell Events: 5169 mean_circ: 0.931072
Ran for 96550 frames Cell Events: 5263 mean_circ: 0.931098
Ran for 97050 frames Cell Events: 5282 mean_circ: 0.931078
Ran for 98050 frames Cell Events: 5338 mean_circ: 0.931074
Ran for 99050 frames Cell Events: 5408 mean_circ: 0.931085
Ran for 99550 frames Cell Events: 5431 mean_circ: 0.931079
Ran for 100050 frames Cell Events: 5455 mean_circ: 0.931063
Ran for 102550 frames Cell Events: 5640 mean_circ: 0.931003
Ran for 103550 frames Cell Events: 5686 mean_circ: 0.93097
Ran for 104050 frames Cell Events: 5710 mean_circ: 0.930914
Ran for 104550 frames Cell Events: 5737 mean_circ: 0.930947
Ran for 105550 frames Cell Events: 5782 mean_circ: 0.930897
Ran for 107550 frames Cell Events: 5923 mean_circ: 0.930941
Ran for 109050 frames Cell Events: 6023 mean_circ: 0.931017
Ran for 109550 frames Cell Events: 6051 mean_circ: 0.931012
Ran for 110550 frames Cell Events: 6110 mean_circ: 0.930971
Ran for 111050 frames Cell Events: 6122 mean_circ: 0.930939
Ran for 113550 frames Cell Events: 6278 mean_circ: 0.930899
Ran for 114550 frames Cell Events: 6346 mean_circ: 0.930932
Ran for 115050 frames Cell Events: 6377 mean_circ: 0.930866
Ran for 115550 frames Cell Events: 6414 mean_circ: 0.93091
Ran for 116050 frames Cell Events: 6439 mean_circ: 0.930957
Ran for 116550 frames Cell Events: 6463 mean_circ: 0.930966
Ran for 117550 frames Cell Events: 6512 mean_circ: 0.930897
Ran for 121550 frames Cell Events: 6737 mean_circ: 0.930721
Ran for 122050 frames Cell Events: 6761 mean_circ: 0.930769
Ran for 123050 frames Cell Events: 6817 mean_circ: 0.930788
Ran for 125550 frames Cell Events: 6972 mean_circ: 0.930897
Ran for 127050 frames Cell Events: 7054 mean_circ: 0.930937
Ran for 127550 frames Cell Events: 7079 mean_circ: 0.930936
Ran for 128050 frames Cell Events: 7098 mean_circ: 0.930975
Ran for 129050 frames Cell Events: 7156 mean_circ: 0.930992
Ran for 131550 frames Cell Events: 7297 mean_circ: 0.930946
Ran for 132050 frames Cell Events: 7321 mean_circ: 0.930949
Ran for 133050 frames Cell Events: 7342 mean_circ: 0.930954
Ran for 134050 frames Cell Events: 7398 mean_circ: 0.930993
Ran for 134550 frames Cell Events: 7424 mean_circ: 0.930991
Ran for 135050 frames Cell Events: 7452 mean_circ: 0.931011
Ran for 136050 frames Cell Events: 7513 mean_circ: 0.931071
Ran for 137550 frames Cell Events: 7611 mean_circ: 0.931114
Ran for 140550 frames Cell Events: 7743 mean_circ: 0.931025
Ran for 141550 frames Cell Events: 7784 mean_circ: 0.930963
Ran for 142050 frames Cell Events: 7806 mean_circ: 0.93099
Ran for 143050 frames Cell Events: 7864 mean_circ: 0.931047
Ran for 143550 frames Cell Events: 7890 mean_circ: 0.931067
Ran for 144550 frames Cell Events: 7963 mean_circ: 0.930945
Ran for 146550 frames Cell Events: 8079 mean_circ: 0.931043



End this measurment..



Stopping time: 1.07751e+008
****************************************
Measurement finished

Mean Circularitiy: 0.931027
Mean Area: 656.637
Mean FL-1 intensity: 3833.1
Mean FL-2 intensity: 5093.28
Mean FL-3 intensity: 0

frames processed: 143738
Total Time: 73507.1ms
Number of Runs (new bg img) 0
Frames collected: 146800
Skipped frames: 24643
Rescued Frames: 1265000
Samples DAQ: 36699968
Samples written: 73399726
Samples per Channel: 36699863
FPS: 1997.09

Samples per second: 499270
Expected samples/s: 500000

Events: 8095
Events per second: 110.125
jumped back (PMT): 32
#Frames read, frequency
# 0 frames :228223
# 1 frames :53729
# 2 frames :2591
# 3 frames :924
# 4 frames :529
# 5 frames :274
# 6 frames :1671
# 7 frames :1195
# 8 frames :873
# 9 frames :528
# 10 frames :386
# 11 frames :333
# 12 frames :307
# 13 frames :211
# 14 frames :258
# 15 frames :206
# 16 frames :202
# 17 frames :165
# 18 frames :146
# 19 frames :107
# 20 frames :85
# 21 frames :77
# 22 frames :60
# 23 frames :59
# 24 frames :45
# 25 frames :34
# 26 frames :34
# 27 frames :44
# 28 frames :26
# 29 frames :29
# 30 frames :23
# 31 frames :14
# 32 frames :16
# 33 frames :13
# 34 frames :14
# 35 frames :11
# 36 frames :8
# 37 frames :13
# 38 frames :5
# 39 frames :9
# 40 frames :4
# 41 frames :4
# 42 frames :3
# 43 frames :2
# 44 frames :1
# 45 frames :2
# 46 frames :3
# 47 frames :3
# 48 frames :3
# 49 frames :2
# 50 frames :2
# 51 frames :4
# 52 frames :5
# 54 frames :2
# 56 frames :1
# 57 frames :1
# 59 frames :1
# 62 frames :1
# 65 frames :1
# 70 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.5429
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0