Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ccdf6b0d8b0920e54b285af07f5a5a0b",
"name": "M1_20170516_1444_dsRNAE3_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:34:28"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.6598
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.09299e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.943856
Ran for 1050 frames Cell Events: 48 mean_circ: 0.942816
Ran for 1550 frames Cell Events: 73 mean_circ: 0.937603
Ran for 2050 frames Cell Events: 90 mean_circ: 0.937863
Ran for 3550 frames Cell Events: 168 mean_circ: 0.931498
Ran for 4050 frames Cell Events: 203 mean_circ: 0.930868
Ran for 6550 frames Cell Events: 350 mean_circ: 0.930987
Ran for 7050 frames Cell Events: 377 mean_circ: 0.930671
Ran for 7550 frames Cell Events: 401 mean_circ: 0.930429
Ran for 8050 frames Cell Events: 435 mean_circ: 0.930649
Ran for 8550 frames Cell Events: 456 mean_circ: 0.931378
Ran for 9050 frames Cell Events: 490 mean_circ: 0.931357
Ran for 10050 frames Cell Events: 544 mean_circ: 0.931301
Ran for 10550 frames Cell Events: 575 mean_circ: 0.931971
Ran for 11050 frames Cell Events: 608 mean_circ: 0.932468
Ran for 12050 frames Cell Events: 683 mean_circ: 0.931962
Ran for 12550 frames Cell Events: 713 mean_circ: 0.931584
Ran for 13050 frames Cell Events: 732 mean_circ: 0.931647
Ran for 13550 frames Cell Events: 760 mean_circ: 0.931515
Ran for 15550 frames Cell Events: 888 mean_circ: 0.930933
Ran for 16550 frames Cell Events: 944 mean_circ: 0.931057
Ran for 17550 frames Cell Events: 988 mean_circ: 0.931437
Ran for 21050 frames Cell Events: 1177 mean_circ: 0.932085
Ran for 22050 frames Cell Events: 1231 mean_circ: 0.932623
Ran for 22550 frames Cell Events: 1254 mean_circ: 0.932333
Ran for 24550 frames Cell Events: 1382 mean_circ: 0.932235
Ran for 25050 frames Cell Events: 1425 mean_circ: 0.932341
Ran for 25550 frames Cell Events: 1455 mean_circ: 0.93228
Ran for 26050 frames Cell Events: 1482 mean_circ: 0.932448
Ran for 26550 frames Cell Events: 1521 mean_circ: 0.932512
Ran for 27550 frames Cell Events: 1604 mean_circ: 0.932667
Ran for 30550 frames Cell Events: 1829 mean_circ: 0.932741
Ran for 31050 frames Cell Events: 1866 mean_circ: 0.932774
Ran for 32050 frames Cell Events: 1927 mean_circ: 0.932771
Ran for 32550 frames Cell Events: 1957 mean_circ: 0.932815
Ran for 33050 frames Cell Events: 1981 mean_circ: 0.932767
Ran for 35050 frames Cell Events: 2074 mean_circ: 0.932704
Ran for 36550 frames Cell Events: 2154 mean_circ: 0.932095
Ran for 37050 frames Cell Events: 2188 mean_circ: 0.932186
Ran for 38050 frames Cell Events: 2235 mean_circ: 0.932307
Ran for 39550 frames Cell Events: 2312 mean_circ: 0.932193
Ran for 40050 frames Cell Events: 2341 mean_circ: 0.932301
Ran for 43050 frames Cell Events: 2517 mean_circ: 0.932284
Ran for 43550 frames Cell Events: 2536 mean_circ: 0.932245
Ran for 44050 frames Cell Events: 2568 mean_circ: 0.931936
Ran for 45550 frames Cell Events: 2685 mean_circ: 0.932066
Ran for 50050 frames Cell Events: 2980 mean_circ: 0.93213
Ran for 51550 frames Cell Events: 3035 mean_circ: 0.932121
Ran for 52550 frames Cell Events: 3086 mean_circ: 0.931901
Ran for 53550 frames Cell Events: 3146 mean_circ: 0.931904
Ran for 54050 frames Cell Events: 3175 mean_circ: 0.93202
Ran for 55550 frames Cell Events: 3239 mean_circ: 0.932085
Ran for 56050 frames Cell Events: 3267 mean_circ: 0.932146
Ran for 57050 frames Cell Events: 3322 mean_circ: 0.932308
Ran for 57550 frames Cell Events: 3342 mean_circ: 0.932314
Ran for 58050 frames Cell Events: 3372 mean_circ: 0.932309
Ran for 58550 frames Cell Events: 3409 mean_circ: 0.932351
Ran for 59050 frames Cell Events: 3439 mean_circ: 0.93241
Ran for 60050 frames Cell Events: 3533 mean_circ: 0.932393
Ran for 60550 frames Cell Events: 3558 mean_circ: 0.932435
Ran for 62050 frames Cell Events: 3654 mean_circ: 0.932331
Ran for 62550 frames Cell Events: 3669 mean_circ: 0.932392
Ran for 64050 frames Cell Events: 3790 mean_circ: 0.93255
Ran for 66050 frames Cell Events: 3899 mean_circ: 0.932369
Ran for 66550 frames Cell Events: 3930 mean_circ: 0.93234
Ran for 67550 frames Cell Events: 4002 mean_circ: 0.932472
Ran for 68050 frames Cell Events: 4030 mean_circ: 0.932419
Ran for 68550 frames Cell Events: 4055 mean_circ: 0.932336
Ran for 70050 frames Cell Events: 4144 mean_circ: 0.932245
Ran for 70550 frames Cell Events: 4173 mean_circ: 0.932238
Ran for 71550 frames Cell Events: 4252 mean_circ: 0.932311
Ran for 72550 frames Cell Events: 4323 mean_circ: 0.932418
Ran for 73550 frames Cell Events: 4379 mean_circ: 0.932527
Ran for 74050 frames Cell Events: 4413 mean_circ: 0.932687
Ran for 76050 frames Cell Events: 4532 mean_circ: 0.932823
Ran for 78550 frames Cell Events: 4675 mean_circ: 0.9329
Ran for 79050 frames Cell Events: 4694 mean_circ: 0.932919
Ran for 79550 frames Cell Events: 4718 mean_circ: 0.932915
Ran for 80050 frames Cell Events: 4743 mean_circ: 0.932798
Ran for 81050 frames Cell Events: 4810 mean_circ: 0.932796
Ran for 83550 frames Cell Events: 4961 mean_circ: 0.932802
Ran for 85050 frames Cell Events: 5050 mean_circ: 0.9328
Ran for 86050 frames Cell Events: 5098 mean_circ: 0.932696
Ran for 88050 frames Cell Events: 5201 mean_circ: 0.932691
Ran for 89550 frames Cell Events: 5265 mean_circ: 0.932719
Ran for 91550 frames Cell Events: 5401 mean_circ: 0.932692
Ran for 92550 frames Cell Events: 5450 mean_circ: 0.932703
Ran for 93050 frames Cell Events: 5470 mean_circ: 0.932753
Ran for 93550 frames Cell Events: 5500 mean_circ: 0.932759
Ran for 94050 frames Cell Events: 5531 mean_circ: 0.932696
Ran for 94550 frames Cell Events: 5567 mean_circ: 0.932665
Ran for 96050 frames Cell Events: 5653 mean_circ: 0.932709
Ran for 97550 frames Cell Events: 5748 mean_circ: 0.93256
Ran for 98050 frames Cell Events: 5779 mean_circ: 0.932614
Ran for 98550 frames Cell Events: 5807 mean_circ: 0.93262
Ran for 99550 frames Cell Events: 5869 mean_circ: 0.932541
Ran for 102550 frames Cell Events: 6044 mean_circ: 0.932576
Ran for 103550 frames Cell Events: 6106 mean_circ: 0.93248
Ran for 106050 frames Cell Events: 6252 mean_circ: 0.932408
Ran for 107550 frames Cell Events: 6347 mean_circ: 0.932423
Ran for 108550 frames Cell Events: 6418 mean_circ: 0.932339
Ran for 109050 frames Cell Events: 6442 mean_circ: 0.932353
Ran for 110050 frames Cell Events: 6494 mean_circ: 0.932389
Ran for 111550 frames Cell Events: 6605 mean_circ: 0.932278
Ran for 112550 frames Cell Events: 6671 mean_circ: 0.932233
Ran for 113050 frames Cell Events: 6690 mean_circ: 0.932273
Ran for 114050 frames Cell Events: 6749 mean_circ: 0.932248
Ran for 116550 frames Cell Events: 6898 mean_circ: 0.932205
Ran for 119050 frames Cell Events: 7074 mean_circ: 0.932141
Ran for 120550 frames Cell Events: 7162 mean_circ: 0.932102
Ran for 121550 frames Cell Events: 7236 mean_circ: 0.932089
Ran for 122050 frames Cell Events: 7269 mean_circ: 0.932095
Ran for 123550 frames Cell Events: 7362 mean_circ: 0.932081
Ran for 124050 frames Cell Events: 7384 mean_circ: 0.932127
Ran for 124550 frames Cell Events: 7410 mean_circ: 0.932114
Ran for 125550 frames Cell Events: 7480 mean_circ: 0.932021
Ran for 126050 frames Cell Events: 7503 mean_circ: 0.932026
Ran for 126550 frames Cell Events: 7533 mean_circ: 0.932052
Ran for 127550 frames Cell Events: 7597 mean_circ: 0.932015
Ran for 129050 frames Cell Events: 7675 mean_circ: 0.932005
Ran for 130050 frames Cell Events: 7734 mean_circ: 0.932
Ran for 131050 frames Cell Events: 7789 mean_circ: 0.932052
Ran for 132050 frames Cell Events: 7850 mean_circ: 0.932132
Ran for 137050 frames Cell Events: 8236 mean_circ: 0.932195
Ran for 138550 frames Cell Events: 8331 mean_circ: 0.932184
Ran for 140550 frames Cell Events: 8484 mean_circ: 0.932192
Ran for 141050 frames Cell Events: 8518 mean_circ: 0.932118
Ran for 142050 frames Cell Events: 8584 mean_circ: 0.932155
Ran for 143050 frames Cell Events: 8658 mean_circ: 0.932203
Ran for 144050 frames Cell Events: 8721 mean_circ: 0.932197
Ran for 145550 frames Cell Events: 8817 mean_circ: 0.932239
Ran for 146550 frames Cell Events: 8890 mean_circ: 0.932279
Ran for 147050 frames Cell Events: 8918 mean_circ: 0.932239
Ran for 147550 frames Cell Events: 8947 mean_circ: 0.932237
Ran for 148550 frames Cell Events: 8997 mean_circ: 0.932249
Ran for 150050 frames Cell Events: 9093 mean_circ: 0.932267
Ran for 150550 frames Cell Events: 9121 mean_circ: 0.932269
Ran for 153550 frames Cell Events: 9363 mean_circ: 0.932362
Ran for 154050 frames Cell Events: 9389 mean_circ: 0.932398
Ran for 155050 frames Cell Events: 9444 mean_circ: 0.93239
Ran for 156050 frames Cell Events: 9506 mean_circ: 0.932407
Ran for 157050 frames Cell Events: 9562 mean_circ: 0.932403
Ran for 158050 frames Cell Events: 9622 mean_circ: 0.932364
Ran for 159550 frames Cell Events: 9710 mean_circ: 0.932329
Ran for 161050 frames Cell Events: 9801 mean_circ: 0.932394
Ran for 161550 frames Cell Events: 9826 mean_circ: 0.932389
Ran for 162050 frames Cell Events: 9848 mean_circ: 0.932398
Ran for 163050 frames Cell Events: 9907 mean_circ: 0.932408



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.09381e+008
****************************************
Measurement finished

Mean Circularitiy: 0.932394
Mean Area: 646.823
Mean FL-1 intensity: 5299.84
Mean FL-2 intensity: 7082.91
Mean FL-3 intensity: 0

frames processed: 160834
Total Time: 82417.7ms
Number of Runs (new bg img) 0
Frames collected: 164709
Skipped frames: 33926
Rescued Frames: 1643051
Samples DAQ: 41180085
Samples written: 82354158
Samples per Channel: 41177079
FPS: 1998.47

Samples per second: 499615
Expected samples/s: 500000

Events: 10002
Events per second: 121.357
jumped back (PMT): 30
#Frames read, frequency
# 0 frames :259599
# 1 frames :55629
# 2 frames :3213
# 3 frames :1076
# 4 frames :588
# 5 frames :268
# 6 frames :2214
# 7 frames :1438
# 8 frames :1204
# 9 frames :827
# 10 frames :512
# 11 frames :390
# 12 frames :301
# 13 frames :224
# 14 frames :306
# 15 frames :232
# 16 frames :212
# 17 frames :165
# 18 frames :133
# 19 frames :94
# 20 frames :72
# 21 frames :73
# 22 frames :70
# 23 frames :59
# 24 frames :48
# 25 frames :39
# 26 frames :36
# 27 frames :27
# 28 frames :19
# 29 frames :19
# 30 frames :20
# 31 frames :21
# 32 frames :14
# 33 frames :14
# 34 frames :13
# 35 frames :14
# 36 frames :11
# 37 frames :6
# 38 frames :5
# 39 frames :10
# 40 frames :6
# 41 frames :6
# 42 frames :4
# 43 frames :3
# 44 frames :4
# 45 frames :4
# 46 frames :7
# 47 frames :9
# 48 frames :5
# 49 frames :1
# 50 frames :3
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :1
# 55 frames :2
# 56 frames :1
# 57 frames :2
# 59 frames :2
# 60 frames :1
# 61 frames :2
# 62 frames :2
# 66 frames :1
# 69 frames :1
# 78 frames :1
# 83 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.6598
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0