Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "1bb3359e8084906ecadca009ffcb36a4",
"name": "M1_20170516_1545_dsRNA_E8_col.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:38:23"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8691 (max 8692) in '8691'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.8145
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.12955e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 3 mean_circ: 0.926732
Ran for 2050 frames Cell Events: 133 mean_circ: 0.946996
Ran for 2550 frames Cell Events: 158 mean_circ: 0.941357
Ran for 4050 frames Cell Events: 284 mean_circ: 0.938634
Ran for 5050 frames Cell Events: 354 mean_circ: 0.941084
Ran for 5550 frames Cell Events: 384 mean_circ: 0.941066
Ran for 6050 frames Cell Events: 404 mean_circ: 0.941349
Ran for 8050 frames Cell Events: 505 mean_circ: 0.942032
Ran for 8550 frames Cell Events: 534 mean_circ: 0.941833
Ran for 10050 frames Cell Events: 590 mean_circ: 0.941641
Ran for 10550 frames Cell Events: 606 mean_circ: 0.942137
Ran for 11050 frames Cell Events: 629 mean_circ: 0.942283
Ran for 12050 frames Cell Events: 684 mean_circ: 0.941655
Ran for 13050 frames Cell Events: 747 mean_circ: 0.942079
Ran for 14050 frames Cell Events: 812 mean_circ: 0.942189
Ran for 14550 frames Cell Events: 845 mean_circ: 0.942046
Ran for 15050 frames Cell Events: 872 mean_circ: 0.941946
Ran for 15550 frames Cell Events: 908 mean_circ: 0.942082
Ran for 16550 frames Cell Events: 963 mean_circ: 0.942105
Ran for 17550 frames Cell Events: 1049 mean_circ: 0.942212
Ran for 19550 frames Cell Events: 1195 mean_circ: 0.942081
Ran for 21050 frames Cell Events: 1305 mean_circ: 0.941821
Ran for 24050 frames Cell Events: 1489 mean_circ: 0.941865
Ran for 24550 frames Cell Events: 1526 mean_circ: 0.941616
Ran for 25050 frames Cell Events: 1565 mean_circ: 0.941583
Ran for 26050 frames Cell Events: 1624 mean_circ: 0.941836
Ran for 26550 frames Cell Events: 1650 mean_circ: 0.941903
Ran for 27050 frames Cell Events: 1675 mean_circ: 0.941891
Ran for 27550 frames Cell Events: 1696 mean_circ: 0.941842
Ran for 28050 frames Cell Events: 1714 mean_circ: 0.941829
Ran for 29050 frames Cell Events: 1772 mean_circ: 0.941801
Ran for 31550 frames Cell Events: 1909 mean_circ: 0.94245
Ran for 32550 frames Cell Events: 1986 mean_circ: 0.942155
Ran for 33050 frames Cell Events: 2021 mean_circ: 0.94234
Ran for 33550 frames Cell Events: 2048 mean_circ: 0.942516
Ran for 34550 frames Cell Events: 2120 mean_circ: 0.942428
Ran for 36050 frames Cell Events: 2214 mean_circ: 0.942279
Ran for 36550 frames Cell Events: 2243 mean_circ: 0.942433
Ran for 37050 frames Cell Events: 2276 mean_circ: 0.942468
Ran for 38050 frames Cell Events: 2317 mean_circ: 0.942376
Ran for 39550 frames Cell Events: 2415 mean_circ: 0.942592
Ran for 40050 frames Cell Events: 2436 mean_circ: 0.942579
Ran for 41050 frames Cell Events: 2500 mean_circ: 0.942913
Ran for 41550 frames Cell Events: 2515 mean_circ: 0.943022
Ran for 44050 frames Cell Events: 2690 mean_circ: 0.942778
Ran for 45050 frames Cell Events: 2754 mean_circ: 0.942916
Ran for 45550 frames Cell Events: 2778 mean_circ: 0.942976
Ran for 49550 frames Cell Events: 3065 mean_circ: 0.942925
Ran for 51550 frames Cell Events: 3167 mean_circ: 0.943044
Ran for 52050 frames Cell Events: 3193 mean_circ: 0.943094
Ran for 52550 frames Cell Events: 3213 mean_circ: 0.943038
Ran for 54550 frames Cell Events: 3346 mean_circ: 0.943518
Ran for 55050 frames Cell Events: 3376 mean_circ: 0.943506
Ran for 55550 frames Cell Events: 3406 mean_circ: 0.94357
Ran for 57550 frames Cell Events: 3495 mean_circ: 0.943655
Ran for 58050 frames Cell Events: 3521 mean_circ: 0.943604
Ran for 59050 frames Cell Events: 3584 mean_circ: 0.943508
Ran for 59550 frames Cell Events: 3610 mean_circ: 0.943546
Ran for 60050 frames Cell Events: 3638 mean_circ: 0.943599
Ran for 61050 frames Cell Events: 3701 mean_circ: 0.943516
Ran for 63550 frames Cell Events: 3859 mean_circ: 0.943198
Ran for 64550 frames Cell Events: 3916 mean_circ: 0.94323
Ran for 65050 frames Cell Events: 3950 mean_circ: 0.943211
Ran for 67050 frames Cell Events: 4096 mean_circ: 0.943005
Ran for 67550 frames Cell Events: 4136 mean_circ: 0.94304
Ran for 68550 frames Cell Events: 4184 mean_circ: 0.943031
Ran for 73550 frames Cell Events: 4603 mean_circ: 0.942914
Ran for 74550 frames Cell Events: 4702 mean_circ: 0.942914
Ran for 76050 frames Cell Events: 4860 mean_circ: 0.942178
Ran for 78050 frames Cell Events: 5031 mean_circ: 0.942057
Ran for 81050 frames Cell Events: 5201 mean_circ: 0.942144
Ran for 81550 frames Cell Events: 5230 mean_circ: 0.942183
Ran for 82550 frames Cell Events: 5281 mean_circ: 0.942189
Ran for 84550 frames Cell Events: 5396 mean_circ: 0.942175
Ran for 85050 frames Cell Events: 5428 mean_circ: 0.942219
Ran for 89050 frames Cell Events: 5686 mean_circ: 0.942425
Ran for 90050 frames Cell Events: 5758 mean_circ: 0.942371
Ran for 91050 frames Cell Events: 5809 mean_circ: 0.942349
Ran for 92050 frames Cell Events: 5867 mean_circ: 0.942346
Ran for 93050 frames Cell Events: 5918 mean_circ: 0.942324
Ran for 95050 frames Cell Events: 6071 mean_circ: 0.942242
Ran for 95550 frames Cell Events: 6098 mean_circ: 0.942227
Ran for 96050 frames Cell Events: 6118 mean_circ: 0.942253
Ran for 96550 frames Cell Events: 6140 mean_circ: 0.942273
Ran for 99050 frames Cell Events: 6293 mean_circ: 0.94232
Ran for 99550 frames Cell Events: 6316 mean_circ: 0.942313
Ran for 100050 frames Cell Events: 6337 mean_circ: 0.942337
Ran for 100550 frames Cell Events: 6378 mean_circ: 0.94238
Ran for 101050 frames Cell Events: 6399 mean_circ: 0.942402
Ran for 101550 frames Cell Events: 6436 mean_circ: 0.942377
Ran for 102550 frames Cell Events: 6492 mean_circ: 0.942444
Ran for 104050 frames Cell Events: 6578 mean_circ: 0.942474
Ran for 104550 frames Cell Events: 6612 mean_circ: 0.942376
Ran for 105550 frames Cell Events: 6661 mean_circ: 0.942418
Ran for 106050 frames Cell Events: 6690 mean_circ: 0.942422
Ran for 107050 frames Cell Events: 6742 mean_circ: 0.942374
Ran for 107550 frames Cell Events: 6757 mean_circ: 0.942385
Ran for 108050 frames Cell Events: 6795 mean_circ: 0.942401
Ran for 109050 frames Cell Events: 6843 mean_circ: 0.942452
Ran for 109550 frames Cell Events: 6875 mean_circ: 0.942448
Ran for 110550 frames Cell Events: 6939 mean_circ: 0.942449
Ran for 111050 frames Cell Events: 6979 mean_circ: 0.942437
Ran for 112050 frames Cell Events: 7042 mean_circ: 0.942338
Ran for 113050 frames Cell Events: 7097 mean_circ: 0.942382
Ran for 115050 frames Cell Events: 7226 mean_circ: 0.942418
Ran for 116050 frames Cell Events: 7295 mean_circ: 0.942453
Ran for 117050 frames Cell Events: 7366 mean_circ: 0.942405
Ran for 117550 frames Cell Events: 7386 mean_circ: 0.942426
Ran for 118050 frames Cell Events: 7413 mean_circ: 0.942453
Ran for 120050 frames Cell Events: 7554 mean_circ: 0.942464
Ran for 120550 frames Cell Events: 7578 mean_circ: 0.942429
Ran for 121050 frames Cell Events: 7604 mean_circ: 0.942403
Ran for 122050 frames Cell Events: 7673 mean_circ: 0.9425
Ran for 123050 frames Cell Events: 7727 mean_circ: 0.942489
Ran for 126050 frames Cell Events: 7966 mean_circ: 0.94249
Ran for 128550 frames Cell Events: 8134 mean_circ: 0.942567
Ran for 129050 frames Cell Events: 8165 mean_circ: 0.94258
Ran for 129550 frames Cell Events: 8210 mean_circ: 0.94254
Ran for 130050 frames Cell Events: 8231 mean_circ: 0.942576
Ran for 130550 frames Cell Events: 8255 mean_circ: 0.942568
Ran for 131550 frames Cell Events: 8289 mean_circ: 0.942491
Ran for 132050 frames Cell Events: 8314 mean_circ: 0.942489
Ran for 133050 frames Cell Events: 8366 mean_circ: 0.942553
Ran for 134550 frames Cell Events: 8447 mean_circ: 0.942584
Ran for 135050 frames Cell Events: 8469 mean_circ: 0.942619
Ran for 135550 frames Cell Events: 8494 mean_circ: 0.942651
Ran for 136050 frames Cell Events: 8516 mean_circ: 0.942659



End this measurment..



Stopping time: 1.13024e+008
****************************************
Measurement finished

Mean Circularitiy: 0.942689
Mean Area: 614.376
Mean FL-1 intensity: 3826.89
Mean FL-2 intensity: 4843.12
Mean FL-3 intensity: 0

frames processed: 135780
Total Time: 69177.5ms
Number of Runs (new bg img) 0
Frames collected: 138150
Skipped frames: 50916
Rescued Frames: 2393024
Samples DAQ: 34537399
Samples written: 69074728
Samples per Channel: 34537364
FPS: 1997.04

Samples per second: 499257
Expected samples/s: 500000

Events: 8692
Events per second: 125.648
jumped back (PMT): 2939
#Frames read, frequency
# 0 frames :217814
# 1 frames :44135
# 2 frames :2638
# 3 frames :908
# 4 frames :572
# 5 frames :233
# 6 frames :1276
# 7 frames :1128
# 8 frames :849
# 9 frames :602
# 10 frames :722
# 11 frames :384
# 12 frames :266
# 13 frames :149
# 14 frames :169
# 15 frames :208
# 16 frames :207
# 17 frames :140
# 18 frames :113
# 19 frames :85
# 20 frames :74
# 21 frames :74
# 22 frames :66
# 23 frames :67
# 24 frames :55
# 25 frames :48
# 26 frames :35
# 27 frames :34
# 28 frames :39
# 29 frames :19
# 30 frames :22
# 31 frames :23
# 32 frames :33
# 33 frames :15
# 34 frames :19
# 35 frames :13
# 36 frames :24
# 37 frames :11
# 38 frames :14
# 39 frames :12
# 40 frames :12
# 41 frames :5
# 42 frames :10
# 43 frames :6
# 44 frames :9
# 45 frames :2
# 46 frames :4
# 47 frames :2
# 48 frames :3
# 49 frames :5
# 50 frames :4
# 51 frames :3
# 52 frames :2
# 53 frames :6
# 54 frames :3
# 55 frames :1
# 56 frames :3
# 57 frames :2
# 58 frames :2
# 59 frames :1
# 60 frames :3
# 61 frames :2
# 62 frames :2
# 66 frames :1
# 70 frames :1
# 71 frames :1
# 75 frames :1
# 77 frames :1
# 78 frames :1
# 85 frames :1
# 86 frames :1
# 87 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.8145
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0