Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "678d6e75fda190b6042d199a543e62f9",
"name": "M1_20170516_1547_dsRNA_E8_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:38:50"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8475 (max 8476) in '8475'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.8218
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.13115e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 41 mean_circ: 0.930791
Ran for 2050 frames Cell Events: 112 mean_circ: 0.924806
Ran for 2550 frames Cell Events: 142 mean_circ: 0.928225
Ran for 3550 frames Cell Events: 209 mean_circ: 0.932805
Ran for 4550 frames Cell Events: 265 mean_circ: 0.932908
Ran for 6050 frames Cell Events: 359 mean_circ: 0.932664
Ran for 7050 frames Cell Events: 413 mean_circ: 0.93419
Ran for 8050 frames Cell Events: 465 mean_circ: 0.934026
Ran for 8550 frames Cell Events: 491 mean_circ: 0.934026
Ran for 9050 frames Cell Events: 519 mean_circ: 0.933763
Ran for 11050 frames Cell Events: 643 mean_circ: 0.934442
Ran for 11550 frames Cell Events: 669 mean_circ: 0.934557
Ran for 12050 frames Cell Events: 692 mean_circ: 0.935099
Ran for 13050 frames Cell Events: 743 mean_circ: 0.935265
Ran for 13550 frames Cell Events: 775 mean_circ: 0.93478
Ran for 14550 frames Cell Events: 849 mean_circ: 0.934446
Ran for 15050 frames Cell Events: 872 mean_circ: 0.934472
Ran for 15550 frames Cell Events: 903 mean_circ: 0.934648
Ran for 16550 frames Cell Events: 987 mean_circ: 0.93418
Ran for 18550 frames Cell Events: 1108 mean_circ: 0.93386
Ran for 19550 frames Cell Events: 1155 mean_circ: 0.933561
Ran for 20050 frames Cell Events: 1198 mean_circ: 0.933684
Ran for 21050 frames Cell Events: 1257 mean_circ: 0.933728
Ran for 22050 frames Cell Events: 1326 mean_circ: 0.93322
Ran for 24050 frames Cell Events: 1438 mean_circ: 0.933169
Ran for 24550 frames Cell Events: 1465 mean_circ: 0.933085
Ran for 26050 frames Cell Events: 1529 mean_circ: 0.933547
Ran for 27550 frames Cell Events: 1639 mean_circ: 0.933851
Ran for 28050 frames Cell Events: 1678 mean_circ: 0.933995
Ran for 29050 frames Cell Events: 1738 mean_circ: 0.934024
Ran for 29550 frames Cell Events: 1763 mean_circ: 0.93395
Ran for 30550 frames Cell Events: 1831 mean_circ: 0.933706
Ran for 31050 frames Cell Events: 1859 mean_circ: 0.933868
Ran for 31550 frames Cell Events: 1880 mean_circ: 0.933799
Ran for 32050 frames Cell Events: 1914 mean_circ: 0.933817
Ran for 33050 frames Cell Events: 1979 mean_circ: 0.934003
Ran for 34050 frames Cell Events: 2025 mean_circ: 0.933991
Ran for 34550 frames Cell Events: 2050 mean_circ: 0.933923
Ran for 37550 frames Cell Events: 2224 mean_circ: 0.933521
Ran for 38550 frames Cell Events: 2281 mean_circ: 0.93361
Ran for 40550 frames Cell Events: 2375 mean_circ: 0.933191
Ran for 41550 frames Cell Events: 2430 mean_circ: 0.933244
Ran for 42050 frames Cell Events: 2454 mean_circ: 0.933025
Ran for 44550 frames Cell Events: 2606 mean_circ: 0.932999
Ran for 45050 frames Cell Events: 2650 mean_circ: 0.933036
Ran for 46050 frames Cell Events: 2708 mean_circ: 0.933225
Ran for 47050 frames Cell Events: 2755 mean_circ: 0.933008
Ran for 47550 frames Cell Events: 2776 mean_circ: 0.933083
Ran for 48050 frames Cell Events: 2798 mean_circ: 0.933129
Ran for 48550 frames Cell Events: 2820 mean_circ: 0.932975
Ran for 49550 frames Cell Events: 2870 mean_circ: 0.932952
Ran for 50050 frames Cell Events: 2898 mean_circ: 0.932964
Ran for 52050 frames Cell Events: 2999 mean_circ: 0.932926
Ran for 54550 frames Cell Events: 3155 mean_circ: 0.932575
Ran for 55550 frames Cell Events: 3191 mean_circ: 0.93261
Ran for 56050 frames Cell Events: 3217 mean_circ: 0.932614
Ran for 57050 frames Cell Events: 3281 mean_circ: 0.932609
Ran for 57550 frames Cell Events: 3322 mean_circ: 0.93248
Ran for 58050 frames Cell Events: 3351 mean_circ: 0.932507
Ran for 61050 frames Cell Events: 3566 mean_circ: 0.932552
Ran for 63050 frames Cell Events: 3689 mean_circ: 0.93261
Ran for 66050 frames Cell Events: 3886 mean_circ: 0.932607
Ran for 69550 frames Cell Events: 4070 mean_circ: 0.932712
Ran for 70550 frames Cell Events: 4117 mean_circ: 0.93271
Ran for 72550 frames Cell Events: 4240 mean_circ: 0.932536
Ran for 73050 frames Cell Events: 4266 mean_circ: 0.932577
Ran for 73550 frames Cell Events: 4286 mean_circ: 0.932578
Ran for 74550 frames Cell Events: 4345 mean_circ: 0.932542
Ran for 75550 frames Cell Events: 4429 mean_circ: 0.932584
Ran for 76550 frames Cell Events: 4492 mean_circ: 0.932674
Ran for 77050 frames Cell Events: 4520 mean_circ: 0.932746
Ran for 78550 frames Cell Events: 4616 mean_circ: 0.932605
Ran for 79050 frames Cell Events: 4631 mean_circ: 0.932648
Ran for 79550 frames Cell Events: 4654 mean_circ: 0.932654
Ran for 81050 frames Cell Events: 4758 mean_circ: 0.932405
Ran for 82050 frames Cell Events: 4829 mean_circ: 0.932371
Ran for 82550 frames Cell Events: 4861 mean_circ: 0.932408
Ran for 83050 frames Cell Events: 4884 mean_circ: 0.932477
Ran for 84050 frames Cell Events: 4926 mean_circ: 0.932596
Ran for 84550 frames Cell Events: 4955 mean_circ: 0.932645
Ran for 85050 frames Cell Events: 4977 mean_circ: 0.932605
Ran for 87050 frames Cell Events: 5112 mean_circ: 0.932455
Ran for 88550 frames Cell Events: 5214 mean_circ: 0.932385
Ran for 89050 frames Cell Events: 5230 mean_circ: 0.93236
Ran for 89550 frames Cell Events: 5251 mean_circ: 0.932376
Ran for 90550 frames Cell Events: 5310 mean_circ: 0.932424
Ran for 93050 frames Cell Events: 5461 mean_circ: 0.932502
Ran for 93550 frames Cell Events: 5500 mean_circ: 0.932544
Ran for 96550 frames Cell Events: 5681 mean_circ: 0.932441
Ran for 97050 frames Cell Events: 5720 mean_circ: 0.932416
Ran for 99050 frames Cell Events: 5845 mean_circ: 0.932345
Ran for 99550 frames Cell Events: 5872 mean_circ: 0.932429
Ran for 100050 frames Cell Events: 5893 mean_circ: 0.932445
Ran for 100550 frames Cell Events: 5916 mean_circ: 0.932467
Ran for 101050 frames Cell Events: 5935 mean_circ: 0.932505
Ran for 101550 frames Cell Events: 5961 mean_circ: 0.932502
Ran for 103050 frames Cell Events: 6058 mean_circ: 0.932578
Ran for 106050 frames Cell Events: 6240 mean_circ: 0.932496
Ran for 106550 frames Cell Events: 6270 mean_circ: 0.932489
Ran for 109050 frames Cell Events: 6407 mean_circ: 0.932488
Ran for 109550 frames Cell Events: 6428 mean_circ: 0.932514
Ran for 110550 frames Cell Events: 6487 mean_circ: 0.932472
Ran for 113050 frames Cell Events: 6638 mean_circ: 0.93247
Ran for 116550 frames Cell Events: 6826 mean_circ: 0.93251
Ran for 117050 frames Cell Events: 6853 mean_circ: 0.932539
Ran for 118550 frames Cell Events: 6928 mean_circ: 0.932579
Ran for 119550 frames Cell Events: 6966 mean_circ: 0.932594
Ran for 120050 frames Cell Events: 7016 mean_circ: 0.932534
Ran for 120550 frames Cell Events: 7058 mean_circ: 0.932517
Ran for 123550 frames Cell Events: 7266 mean_circ: 0.932414
Ran for 125050 frames Cell Events: 7355 mean_circ: 0.932448
Ran for 125550 frames Cell Events: 7388 mean_circ: 0.932453
Ran for 127050 frames Cell Events: 7466 mean_circ: 0.932367
Ran for 127550 frames Cell Events: 7496 mean_circ: 0.932328
Ran for 128050 frames Cell Events: 7510 mean_circ: 0.93233
Ran for 129550 frames Cell Events: 7604 mean_circ: 0.932347
Ran for 131550 frames Cell Events: 7730 mean_circ: 0.932503
Ran for 132050 frames Cell Events: 7752 mean_circ: 0.932441
Ran for 133050 frames Cell Events: 7823 mean_circ: 0.932474
Ran for 133550 frames Cell Events: 7856 mean_circ: 0.932486
Ran for 134050 frames Cell Events: 7884 mean_circ: 0.932441
Ran for 134550 frames Cell Events: 7910 mean_circ: 0.932484
Ran for 135550 frames Cell Events: 7967 mean_circ: 0.93249
Ran for 136550 frames Cell Events: 8019 mean_circ: 0.932467
Ran for 137550 frames Cell Events: 8087 mean_circ: 0.932492
Ran for 138550 frames Cell Events: 8135 mean_circ: 0.932539
Ran for 139050 frames Cell Events: 8156 mean_circ: 0.932563
Ran for 139550 frames Cell Events: 8179 mean_circ: 0.932564
Ran for 141050 frames Cell Events: 8263 mean_circ: 0.932597
Ran for 141550 frames Cell Events: 8303 mean_circ: 0.932597
Ran for 142550 frames Cell Events: 8364 mean_circ: 0.932533
Ran for 143050 frames Cell Events: 8398 mean_circ: 0.932488
Ran for 144050 frames Cell Events: 8451 mean_circ: 0.932505



End this measurment..



Stopping time: 1.13188e+008
****************************************
Measurement finished

Mean Circularitiy: 0.932515
Mean Area: 614.549
Mean FL-1 intensity: 3503.26
Mean FL-2 intensity: 5064.33
Mean FL-3 intensity: 0

frames processed: 141896
Total Time: 72493.2ms
Number of Runs (new bg img) 0
Frames collected: 144752
Skipped frames: 53772
Rescued Frames: 2472699
Samples DAQ: 36190953
Samples written: 72376012
Samples per Channel: 36188006
FPS: 1996.77

Samples per second: 499192
Expected samples/s: 500000

Events: 8476
Events per second: 116.921
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :227044
# 1 frames :50357
# 2 frames :2757
# 3 frames :959
# 4 frames :525
# 5 frames :236
# 6 frames :1566
# 7 frames :1247
# 8 frames :1063
# 9 frames :640
# 10 frames :486
# 11 frames :369
# 12 frames :254
# 13 frames :210
# 14 frames :197
# 15 frames :221
# 16 frames :151
# 17 frames :130
# 18 frames :84
# 19 frames :84
# 20 frames :76
# 21 frames :66
# 22 frames :51
# 23 frames :68
# 24 frames :36
# 25 frames :47
# 26 frames :27
# 27 frames :28
# 28 frames :31
# 29 frames :23
# 30 frames :27
# 31 frames :24
# 32 frames :17
# 33 frames :20
# 34 frames :22
# 35 frames :16
# 36 frames :17
# 37 frames :7
# 38 frames :11
# 39 frames :10
# 40 frames :12
# 41 frames :17
# 42 frames :9
# 43 frames :4
# 44 frames :5
# 45 frames :3
# 46 frames :3
# 47 frames :2
# 48 frames :3
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 52 frames :4
# 54 frames :2
# 55 frames :2
# 56 frames :3
# 57 frames :2
# 58 frames :2
# 60 frames :1
# 62 frames :1
# 63 frames :1
# 64 frames :1
# 65 frames :1
# 71 frames :1
# 72 frames :1
# 73 frames :1
# 83 frames :1
# 88 frames :1
# 97 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.8218
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0