Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "6f57574e448d40424303fbcd132f5e12",
"name": "M1_20170516_1628_dsRNA_F3_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:41:10"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.9132
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.15573e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2550 frames Cell Events: 196 mean_circ: 0.932514
Ran for 3050 frames Cell Events: 227 mean_circ: 0.932658
Ran for 4550 frames Cell Events: 301 mean_circ: 0.933046
Ran for 5050 frames Cell Events: 331 mean_circ: 0.933794
Ran for 5550 frames Cell Events: 358 mean_circ: 0.934331
Ran for 6550 frames Cell Events: 412 mean_circ: 0.934135
Ran for 8550 frames Cell Events: 561 mean_circ: 0.932273
Ran for 11550 frames Cell Events: 720 mean_circ: 0.933072
Ran for 12550 frames Cell Events: 772 mean_circ: 0.932879
Ran for 13050 frames Cell Events: 799 mean_circ: 0.932637
Ran for 15550 frames Cell Events: 942 mean_circ: 0.93261
Ran for 16550 frames Cell Events: 992 mean_circ: 0.931948
Ran for 17550 frames Cell Events: 1032 mean_circ: 0.932132
Ran for 18550 frames Cell Events: 1096 mean_circ: 0.932034
Ran for 19550 frames Cell Events: 1137 mean_circ: 0.932246
Ran for 21550 frames Cell Events: 1253 mean_circ: 0.931619
Ran for 23050 frames Cell Events: 1333 mean_circ: 0.931665
Ran for 24050 frames Cell Events: 1377 mean_circ: 0.931397
Ran for 27050 frames Cell Events: 1530 mean_circ: 0.930858
Ran for 31550 frames Cell Events: 1783 mean_circ: 0.930271
Ran for 32050 frames Cell Events: 1810 mean_circ: 0.930118
Ran for 32550 frames Cell Events: 1844 mean_circ: 0.93015
Ran for 33050 frames Cell Events: 1881 mean_circ: 0.930213
Ran for 33550 frames Cell Events: 1918 mean_circ: 0.930093
Ran for 34550 frames Cell Events: 1980 mean_circ: 0.930256
Ran for 37050 frames Cell Events: 2108 mean_circ: 0.930149
Ran for 38550 frames Cell Events: 2164 mean_circ: 0.930359
Ran for 40050 frames Cell Events: 2253 mean_circ: 0.9299
Ran for 40550 frames Cell Events: 2278 mean_circ: 0.929783
Ran for 41050 frames Cell Events: 2301 mean_circ: 0.929747
Ran for 41550 frames Cell Events: 2333 mean_circ: 0.929382
Ran for 42050 frames Cell Events: 2358 mean_circ: 0.929422
Ran for 42550 frames Cell Events: 2376 mean_circ: 0.929397
Ran for 43050 frames Cell Events: 2404 mean_circ: 0.929385
Ran for 46550 frames Cell Events: 2574 mean_circ: 0.9292
Ran for 47050 frames Cell Events: 2604 mean_circ: 0.929273
Ran for 47550 frames Cell Events: 2624 mean_circ: 0.929187
Ran for 48050 frames Cell Events: 2639 mean_circ: 0.929088
Ran for 48550 frames Cell Events: 2680 mean_circ: 0.929183
Ran for 49050 frames Cell Events: 2707 mean_circ: 0.929255
Ran for 50050 frames Cell Events: 2764 mean_circ: 0.929082
Ran for 50550 frames Cell Events: 2789 mean_circ: 0.929057
Ran for 51550 frames Cell Events: 2855 mean_circ: 0.92896
Ran for 52050 frames Cell Events: 2879 mean_circ: 0.929071
Ran for 53550 frames Cell Events: 2954 mean_circ: 0.928808
Ran for 55550 frames Cell Events: 3050 mean_circ: 0.928896
Ran for 56550 frames Cell Events: 3096 mean_circ: 0.929016
Ran for 57050 frames Cell Events: 3122 mean_circ: 0.929083
Ran for 58050 frames Cell Events: 3167 mean_circ: 0.929056
Ran for 58550 frames Cell Events: 3193 mean_circ: 0.929002
Ran for 59050 frames Cell Events: 3216 mean_circ: 0.929086
Ran for 59550 frames Cell Events: 3244 mean_circ: 0.929195
Ran for 61050 frames Cell Events: 3335 mean_circ: 0.929287
Ran for 61550 frames Cell Events: 3357 mean_circ: 0.929287
Ran for 62050 frames Cell Events: 3395 mean_circ: 0.929197
Ran for 62550 frames Cell Events: 3419 mean_circ: 0.929135
Ran for 63050 frames Cell Events: 3433 mean_circ: 0.929133
Ran for 64050 frames Cell Events: 3496 mean_circ: 0.92917
Ran for 66550 frames Cell Events: 3635 mean_circ: 0.928998
Ran for 67050 frames Cell Events: 3666 mean_circ: 0.928947
Ran for 67550 frames Cell Events: 3693 mean_circ: 0.928997
Ran for 68050 frames Cell Events: 3714 mean_circ: 0.928942
Ran for 68550 frames Cell Events: 3745 mean_circ: 0.928974
Ran for 69050 frames Cell Events: 3781 mean_circ: 0.928959
Ran for 69550 frames Cell Events: 3804 mean_circ: 0.928957
Ran for 71050 frames Cell Events: 3878 mean_circ: 0.928935
Ran for 71550 frames Cell Events: 3915 mean_circ: 0.928961
Ran for 73050 frames Cell Events: 3995 mean_circ: 0.92885
Ran for 75050 frames Cell Events: 4090 mean_circ: 0.928812
Ran for 76050 frames Cell Events: 4152 mean_circ: 0.928685
Ran for 77050 frames Cell Events: 4212 mean_circ: 0.928574
Ran for 78550 frames Cell Events: 4309 mean_circ: 0.928399
Ran for 80050 frames Cell Events: 4392 mean_circ: 0.928491
Ran for 81050 frames Cell Events: 4436 mean_circ: 0.928564
Ran for 81550 frames Cell Events: 4468 mean_circ: 0.928663
Ran for 82050 frames Cell Events: 4496 mean_circ: 0.928642
Ran for 82550 frames Cell Events: 4525 mean_circ: 0.928643
Ran for 83550 frames Cell Events: 4574 mean_circ: 0.928622
Ran for 84550 frames Cell Events: 4631 mean_circ: 0.928657
Ran for 85050 frames Cell Events: 4651 mean_circ: 0.928635
Ran for 86050 frames Cell Events: 4715 mean_circ: 0.928572
Ran for 86550 frames Cell Events: 4736 mean_circ: 0.92854
Ran for 87550 frames Cell Events: 4801 mean_circ: 0.928539
Ran for 88050 frames Cell Events: 4833 mean_circ: 0.928577
Ran for 89050 frames Cell Events: 4889 mean_circ: 0.928667
Ran for 89550 frames Cell Events: 4909 mean_circ: 0.928653
Ran for 90050 frames Cell Events: 4931 mean_circ: 0.928624
Ran for 93050 frames Cell Events: 5101 mean_circ: 0.928418
Ran for 93550 frames Cell Events: 5120 mean_circ: 0.928401
Ran for 95050 frames Cell Events: 5213 mean_circ: 0.928479
Ran for 97050 frames Cell Events: 5341 mean_circ: 0.928501
Ran for 98050 frames Cell Events: 5394 mean_circ: 0.928569
Ran for 100050 frames Cell Events: 5519 mean_circ: 0.928491
Ran for 101550 frames Cell Events: 5597 mean_circ: 0.928548
Ran for 103050 frames Cell Events: 5665 mean_circ: 0.928445
Ran for 105050 frames Cell Events: 5759 mean_circ: 0.928373
Ran for 106050 frames Cell Events: 5808 mean_circ: 0.928401
Ran for 108550 frames Cell Events: 5950 mean_circ: 0.928474
Ran for 109050 frames Cell Events: 5974 mean_circ: 0.928467
Ran for 110550 frames Cell Events: 6078 mean_circ: 0.928396
Ran for 111550 frames Cell Events: 6121 mean_circ: 0.928407
Ran for 112550 frames Cell Events: 6177 mean_circ: 0.928539
Ran for 113550 frames Cell Events: 6233 mean_circ: 0.928515
Ran for 114550 frames Cell Events: 6295 mean_circ: 0.928542
Ran for 115050 frames Cell Events: 6322 mean_circ: 0.928553
Ran for 116050 frames Cell Events: 6364 mean_circ: 0.928495
Ran for 116550 frames Cell Events: 6388 mean_circ: 0.928477
Ran for 118050 frames Cell Events: 6474 mean_circ: 0.928575
Ran for 118550 frames Cell Events: 6494 mean_circ: 0.928605
Ran for 119050 frames Cell Events: 6517 mean_circ: 0.928576
Ran for 120050 frames Cell Events: 6577 mean_circ: 0.928614
Ran for 122050 frames Cell Events: 6685 mean_circ: 0.928546
Ran for 123550 frames Cell Events: 6764 mean_circ: 0.928581
Ran for 124550 frames Cell Events: 6822 mean_circ: 0.928453
Ran for 125050 frames Cell Events: 6860 mean_circ: 0.928477
Ran for 126550 frames Cell Events: 6954 mean_circ: 0.928505
Ran for 128050 frames Cell Events: 7051 mean_circ: 0.928538
Ran for 129050 frames Cell Events: 7113 mean_circ: 0.928604
Ran for 129550 frames Cell Events: 7134 mean_circ: 0.92861
Ran for 130550 frames Cell Events: 7179 mean_circ: 0.928494
Ran for 131050 frames Cell Events: 7207 mean_circ: 0.928555
Ran for 132050 frames Cell Events: 7274 mean_circ: 0.928604
Ran for 132550 frames Cell Events: 7296 mean_circ: 0.928568
Ran for 133050 frames Cell Events: 7325 mean_circ: 0.928571
Ran for 134050 frames Cell Events: 7390 mean_circ: 0.928554
Ran for 134550 frames Cell Events: 7419 mean_circ: 0.928593
Ran for 135050 frames Cell Events: 7445 mean_circ: 0.928578
Ran for 135550 frames Cell Events: 7463 mean_circ: 0.928536
Ran for 137550 frames Cell Events: 7597 mean_circ: 0.928575
Ran for 138050 frames Cell Events: 7621 mean_circ: 0.928582
Ran for 139050 frames Cell Events: 7692 mean_circ: 0.928529
Ran for 141050 frames Cell Events: 7797 mean_circ: 0.928606
Ran for 142050 frames Cell Events: 7851 mean_circ: 0.928591
Ran for 143050 frames Cell Events: 7903 mean_circ: 0.928625
Ran for 143550 frames Cell Events: 7922 mean_circ: 0.928646
Ran for 144550 frames Cell Events: 7989 mean_circ: 0.928561
Ran for 145550 frames Cell Events: 8041 mean_circ: 0.928631
Ran for 146550 frames Cell Events: 8108 mean_circ: 0.92867
Ran for 147050 frames Cell Events: 8137 mean_circ: 0.928663
Ran for 147550 frames Cell Events: 8153 mean_circ: 0.928661
Ran for 148050 frames Cell Events: 8176 mean_circ: 0.928687
Ran for 148550 frames Cell Events: 8192 mean_circ: 0.928684
Ran for 149550 frames Cell Events: 8244 mean_circ: 0.928749
Ran for 150050 frames Cell Events: 8265 mean_circ: 0.928804
Ran for 151550 frames Cell Events: 8365 mean_circ: 0.92876
Ran for 154550 frames Cell Events: 8520 mean_circ: 0.928731
Ran for 155550 frames Cell Events: 8590 mean_circ: 0.92878
Ran for 157550 frames Cell Events: 8693 mean_circ: 0.928813
Ran for 158050 frames Cell Events: 8725 mean_circ: 0.92875
Ran for 158550 frames Cell Events: 8751 mean_circ: 0.928766
Ran for 159550 frames Cell Events: 8815 mean_circ: 0.92864
Ran for 160050 frames Cell Events: 8840 mean_circ: 0.928614
Ran for 160550 frames Cell Events: 8860 mean_circ: 0.928634
Ran for 161050 frames Cell Events: 8895 mean_circ: 0.928618
Ran for 161550 frames Cell Events: 8934 mean_circ: 0.928645
Ran for 164050 frames Cell Events: 9072 mean_circ: 0.928699
Ran for 165050 frames Cell Events: 9128 mean_circ: 0.928647
Ran for 165550 frames Cell Events: 9157 mean_circ: 0.928676
Ran for 166550 frames Cell Events: 9206 mean_circ: 0.92866
Ran for 167550 frames Cell Events: 9259 mean_circ: 0.9287
Ran for 168550 frames Cell Events: 9306 mean_circ: 0.92872
Ran for 169050 frames Cell Events: 9321 mean_circ: 0.928719
Ran for 170050 frames Cell Events: 9376 mean_circ: 0.928715
Ran for 170550 frames Cell Events: 9399 mean_circ: 0.928743
Ran for 171050 frames Cell Events: 9423 mean_circ: 0.928724
Ran for 171550 frames Cell Events: 9455 mean_circ: 0.92873
Ran for 172550 frames Cell Events: 9506 mean_circ: 0.928717
Ran for 173550 frames Cell Events: 9559 mean_circ: 0.928691
Ran for 174050 frames Cell Events: 9581 mean_circ: 0.928691
Ran for 175050 frames Cell Events: 9626 mean_circ: 0.928693
Ran for 175550 frames Cell Events: 9653 mean_circ: 0.928685
Ran for 176550 frames Cell Events: 9723 mean_circ: 0.928702
Ran for 178050 frames Cell Events: 9806 mean_circ: 0.928654
Ran for 179550 frames Cell Events: 9894 mean_circ: 0.928578
Ran for 180050 frames Cell Events: 9921 mean_circ: 0.92852
Ran for 180550 frames Cell Events: 9947 mean_circ: 0.928521
Ran for 181550 frames Cell Events: 9984 mean_circ: 0.928537



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.15664e+008
****************************************
Measurement finished

Mean Circularitiy: 0.928529
Mean Area: 665.014
Mean FL-1 intensity: 3086.9
Mean FL-2 intensity: 5035.81
Mean FL-3 intensity: 0

frames processed: 179766
Total Time: 91049.4ms
Number of Runs (new bg img) 0
Frames collected: 181922
Skipped frames: 64400
Rescued Frames: 3026966
Samples DAQ: 45485302
Samples written: 90961120
Samples per Channel: 45480560
FPS: 1998.06

Samples per second: 499515
Expected samples/s: 500000

Events: 10002
Events per second: 109.852
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :281967
# 1 frames :66786
# 2 frames :3585
# 3 frames :1198
# 4 frames :638
# 5 frames :313
# 6 frames :2093
# 7 frames :1486
# 8 frames :1261
# 9 frames :734
# 10 frames :585
# 11 frames :431
# 12 frames :418
# 13 frames :235
# 14 frames :275
# 15 frames :297
# 16 frames :211
# 17 frames :167
# 18 frames :145
# 19 frames :101
# 20 frames :80
# 21 frames :89
# 22 frames :78
# 23 frames :74
# 24 frames :49
# 25 frames :54
# 26 frames :38
# 27 frames :40
# 28 frames :33
# 29 frames :26
# 30 frames :26
# 31 frames :31
# 32 frames :14
# 33 frames :18
# 34 frames :26
# 35 frames :16
# 36 frames :8
# 37 frames :16
# 38 frames :10
# 39 frames :3
# 40 frames :12
# 41 frames :11
# 42 frames :2
# 43 frames :3
# 44 frames :4
# 45 frames :5
# 46 frames :4
# 47 frames :1
# 48 frames :2
# 49 frames :1
# 50 frames :1
# 51 frames :2
# 52 frames :2
# 53 frames :2
# 54 frames :3
# 55 frames :1
# 56 frames :2
# 58 frames :1
# 60 frames :2
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# 64 frames :1
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# 66 frames :1
# 67 frames :2
# 71 frames :1
# 87 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.9132
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0