Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3f857c177ad8fc0ae1d7368007a9ec8b",
"name": "M1_20170516_1653_dsRNA_LZ_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:42:26"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8135 (max 8136) in '8135'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.9499
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.17066e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 42 mean_circ: 0.919246
Ran for 2550 frames Cell Events: 130 mean_circ: 0.926459
Ran for 4050 frames Cell Events: 191 mean_circ: 0.92565
Ran for 4550 frames Cell Events: 219 mean_circ: 0.924708
Ran for 5050 frames Cell Events: 245 mean_circ: 0.925494
Ran for 6050 frames Cell Events: 294 mean_circ: 0.923383
Ran for 6550 frames Cell Events: 315 mean_circ: 0.922977
Ran for 8050 frames Cell Events: 369 mean_circ: 0.923889
Ran for 8550 frames Cell Events: 395 mean_circ: 0.923904
Ran for 9050 frames Cell Events: 417 mean_circ: 0.924383
Ran for 9550 frames Cell Events: 446 mean_circ: 0.924955
Ran for 10550 frames Cell Events: 498 mean_circ: 0.926084
Ran for 11550 frames Cell Events: 542 mean_circ: 0.925915
Ran for 12550 frames Cell Events: 600 mean_circ: 0.925521
Ran for 13050 frames Cell Events: 622 mean_circ: 0.925907
Ran for 14550 frames Cell Events: 696 mean_circ: 0.925577
Ran for 15550 frames Cell Events: 729 mean_circ: 0.925369
Ran for 16550 frames Cell Events: 782 mean_circ: 0.925724
Ran for 18550 frames Cell Events: 899 mean_circ: 0.926629
Ran for 19050 frames Cell Events: 928 mean_circ: 0.926685
Ran for 20050 frames Cell Events: 982 mean_circ: 0.927409
Ran for 20550 frames Cell Events: 997 mean_circ: 0.92769
Ran for 21050 frames Cell Events: 1011 mean_circ: 0.927509
Ran for 22550 frames Cell Events: 1075 mean_circ: 0.927568
Ran for 23550 frames Cell Events: 1119 mean_circ: 0.928063
Ran for 24050 frames Cell Events: 1142 mean_circ: 0.9281
Ran for 24550 frames Cell Events: 1174 mean_circ: 0.92799
Ran for 25550 frames Cell Events: 1219 mean_circ: 0.928018
Ran for 26050 frames Cell Events: 1256 mean_circ: 0.927998
Ran for 27550 frames Cell Events: 1344 mean_circ: 0.927789
Ran for 28050 frames Cell Events: 1368 mean_circ: 0.927565
Ran for 29550 frames Cell Events: 1443 mean_circ: 0.927934
Ran for 30050 frames Cell Events: 1457 mean_circ: 0.927815
Ran for 30550 frames Cell Events: 1488 mean_circ: 0.927738
Ran for 31050 frames Cell Events: 1508 mean_circ: 0.927291
Ran for 31550 frames Cell Events: 1531 mean_circ: 0.927395
Ran for 32050 frames Cell Events: 1566 mean_circ: 0.927226
Ran for 32550 frames Cell Events: 1583 mean_circ: 0.927186
Ran for 33050 frames Cell Events: 1617 mean_circ: 0.927413
Ran for 33550 frames Cell Events: 1638 mean_circ: 0.927487
Ran for 34050 frames Cell Events: 1662 mean_circ: 0.927292
Ran for 34550 frames Cell Events: 1688 mean_circ: 0.927238
Ran for 35050 frames Cell Events: 1720 mean_circ: 0.927296
Ran for 35550 frames Cell Events: 1741 mean_circ: 0.927344
Ran for 37550 frames Cell Events: 1838 mean_circ: 0.927754
Ran for 38050 frames Cell Events: 1876 mean_circ: 0.927761
Ran for 38550 frames Cell Events: 1899 mean_circ: 0.927885
Ran for 39550 frames Cell Events: 1954 mean_circ: 0.927939
Ran for 40550 frames Cell Events: 2005 mean_circ: 0.927826
Ran for 41050 frames Cell Events: 2038 mean_circ: 0.927836
Ran for 42050 frames Cell Events: 2084 mean_circ: 0.927849
Ran for 43050 frames Cell Events: 2152 mean_circ: 0.927807
Ran for 43550 frames Cell Events: 2185 mean_circ: 0.927883
Ran for 44050 frames Cell Events: 2210 mean_circ: 0.927952
Ran for 45050 frames Cell Events: 2262 mean_circ: 0.928239
Ran for 45550 frames Cell Events: 2281 mean_circ: 0.92837
Ran for 47550 frames Cell Events: 2387 mean_circ: 0.928501
Ran for 48050 frames Cell Events: 2399 mean_circ: 0.928583
Ran for 48550 frames Cell Events: 2425 mean_circ: 0.928643
Ran for 49050 frames Cell Events: 2450 mean_circ: 0.92855
Ran for 50550 frames Cell Events: 2527 mean_circ: 0.928633
Ran for 51550 frames Cell Events: 2587 mean_circ: 0.928882
Ran for 52050 frames Cell Events: 2619 mean_circ: 0.928958
Ran for 52550 frames Cell Events: 2636 mean_circ: 0.929058
Ran for 53550 frames Cell Events: 2680 mean_circ: 0.928907
Ran for 54050 frames Cell Events: 2712 mean_circ: 0.929079
Ran for 55050 frames Cell Events: 2742 mean_circ: 0.928949
Ran for 55550 frames Cell Events: 2767 mean_circ: 0.928949
Ran for 56050 frames Cell Events: 2802 mean_circ: 0.929007
Ran for 57050 frames Cell Events: 2854 mean_circ: 0.928879
Ran for 58550 frames Cell Events: 2911 mean_circ: 0.928825
Ran for 59550 frames Cell Events: 2968 mean_circ: 0.928893
Ran for 61050 frames Cell Events: 3055 mean_circ: 0.928944
Ran for 64550 frames Cell Events: 3318 mean_circ: 0.928486
Ran for 65550 frames Cell Events: 3401 mean_circ: 0.928326
Ran for 66050 frames Cell Events: 3429 mean_circ: 0.928283
Ran for 66550 frames Cell Events: 3455 mean_circ: 0.928293
Ran for 67550 frames Cell Events: 3516 mean_circ: 0.928322
Ran for 68050 frames Cell Events: 3539 mean_circ: 0.92836
Ran for 68550 frames Cell Events: 3574 mean_circ: 0.92836
Ran for 70050 frames Cell Events: 3653 mean_circ: 0.928216
Ran for 71050 frames Cell Events: 3692 mean_circ: 0.928131
Ran for 71550 frames Cell Events: 3709 mean_circ: 0.927985
Ran for 72050 frames Cell Events: 3724 mean_circ: 0.927948
Ran for 75050 frames Cell Events: 3843 mean_circ: 0.927634
Ran for 76050 frames Cell Events: 3871 mean_circ: 0.927681
Ran for 76550 frames Cell Events: 3902 mean_circ: 0.927721
Ran for 77550 frames Cell Events: 3952 mean_circ: 0.927786
Ran for 78050 frames Cell Events: 3981 mean_circ: 0.927854
Ran for 78550 frames Cell Events: 4008 mean_circ: 0.927958
Ran for 79550 frames Cell Events: 4057 mean_circ: 0.928056
Ran for 81550 frames Cell Events: 4169 mean_circ: 0.927906
Ran for 82050 frames Cell Events: 4188 mean_circ: 0.927844
Ran for 82550 frames Cell Events: 4220 mean_circ: 0.927844
Ran for 83550 frames Cell Events: 4269 mean_circ: 0.927765
Ran for 84050 frames Cell Events: 4289 mean_circ: 0.927779
Ran for 85050 frames Cell Events: 4342 mean_circ: 0.92779
Ran for 86550 frames Cell Events: 4406 mean_circ: 0.927769
Ran for 87550 frames Cell Events: 4463 mean_circ: 0.927761
Ran for 88050 frames Cell Events: 4500 mean_circ: 0.927752
Ran for 88550 frames Cell Events: 4527 mean_circ: 0.927738
Ran for 90550 frames Cell Events: 4643 mean_circ: 0.927935
Ran for 92050 frames Cell Events: 4728 mean_circ: 0.927803
Ran for 93550 frames Cell Events: 4797 mean_circ: 0.927772
Ran for 94050 frames Cell Events: 4828 mean_circ: 0.927722
Ran for 94550 frames Cell Events: 4844 mean_circ: 0.927684
Ran for 96050 frames Cell Events: 4931 mean_circ: 0.92776
Ran for 97050 frames Cell Events: 4978 mean_circ: 0.927712
Ran for 98050 frames Cell Events: 5030 mean_circ: 0.927544
Ran for 98550 frames Cell Events: 5046 mean_circ: 0.927569
Ran for 99050 frames Cell Events: 5070 mean_circ: 0.927566
Ran for 100550 frames Cell Events: 5141 mean_circ: 0.927722
Ran for 101050 frames Cell Events: 5162 mean_circ: 0.927716
Ran for 102550 frames Cell Events: 5256 mean_circ: 0.927715
Ran for 103550 frames Cell Events: 5304 mean_circ: 0.927845
Ran for 104050 frames Cell Events: 5326 mean_circ: 0.9279
Ran for 106050 frames Cell Events: 5440 mean_circ: 0.927735
Ran for 106550 frames Cell Events: 5484 mean_circ: 0.927735
Ran for 108050 frames Cell Events: 5565 mean_circ: 0.927862
Ran for 108550 frames Cell Events: 5589 mean_circ: 0.927933
Ran for 109050 frames Cell Events: 5609 mean_circ: 0.927779
Ran for 110050 frames Cell Events: 5654 mean_circ: 0.927749
Ran for 111050 frames Cell Events: 5709 mean_circ: 0.927828
Ran for 111550 frames Cell Events: 5731 mean_circ: 0.927807
Ran for 114050 frames Cell Events: 5865 mean_circ: 0.927745
Ran for 115050 frames Cell Events: 5929 mean_circ: 0.927692
Ran for 115550 frames Cell Events: 5955 mean_circ: 0.927737
Ran for 116550 frames Cell Events: 5992 mean_circ: 0.927823
Ran for 117550 frames Cell Events: 6039 mean_circ: 0.927803
Ran for 119050 frames Cell Events: 6100 mean_circ: 0.927805
Ran for 121050 frames Cell Events: 6217 mean_circ: 0.927795
Ran for 122050 frames Cell Events: 6277 mean_circ: 0.927874
Ran for 123550 frames Cell Events: 6338 mean_circ: 0.927953
Ran for 124050 frames Cell Events: 6360 mean_circ: 0.927942
Ran for 124550 frames Cell Events: 6394 mean_circ: 0.927941
Ran for 125050 frames Cell Events: 6421 mean_circ: 0.928017
Ran for 125550 frames Cell Events: 6443 mean_circ: 0.928095
Ran for 126550 frames Cell Events: 6500 mean_circ: 0.928049
Ran for 128550 frames Cell Events: 6598 mean_circ: 0.928065
Ran for 129050 frames Cell Events: 6615 mean_circ: 0.928074
Ran for 130550 frames Cell Events: 6705 mean_circ: 0.92813
Ran for 131050 frames Cell Events: 6723 mean_circ: 0.928069
Ran for 131550 frames Cell Events: 6749 mean_circ: 0.928053
Ran for 132050 frames Cell Events: 6777 mean_circ: 0.928085
Ran for 133550 frames Cell Events: 6860 mean_circ: 0.928135
Ran for 134050 frames Cell Events: 6883 mean_circ: 0.92814
Ran for 135050 frames Cell Events: 6938 mean_circ: 0.928102
Ran for 136050 frames Cell Events: 6984 mean_circ: 0.928088
Ran for 136550 frames Cell Events: 7011 mean_circ: 0.928025
Ran for 137050 frames Cell Events: 7034 mean_circ: 0.928046
Ran for 137550 frames Cell Events: 7052 mean_circ: 0.928064
Ran for 139050 frames Cell Events: 7148 mean_circ: 0.928029
Ran for 139550 frames Cell Events: 7165 mean_circ: 0.92805
Ran for 141050 frames Cell Events: 7255 mean_circ: 0.928016
Ran for 141550 frames Cell Events: 7274 mean_circ: 0.928022
Ran for 143050 frames Cell Events: 7387 mean_circ: 0.928031
Ran for 144050 frames Cell Events: 7440 mean_circ: 0.928064
Ran for 144550 frames Cell Events: 7464 mean_circ: 0.928061
Ran for 145050 frames Cell Events: 7485 mean_circ: 0.928061
Ran for 146050 frames Cell Events: 7534 mean_circ: 0.927983
Ran for 147050 frames Cell Events: 7590 mean_circ: 0.927955
Ran for 149050 frames Cell Events: 7691 mean_circ: 0.92793
Ran for 149550 frames Cell Events: 7714 mean_circ: 0.927954
Ran for 151050 frames Cell Events: 7787 mean_circ: 0.92803
Ran for 152550 frames Cell Events: 7871 mean_circ: 0.928008
Ran for 154050 frames Cell Events: 7955 mean_circ: 0.927993
Ran for 154550 frames Cell Events: 7974 mean_circ: 0.927991
Ran for 155050 frames Cell Events: 7997 mean_circ: 0.927946
Ran for 157550 frames Cell Events: 8098 mean_circ: 0.928071
Ran for 158050 frames Cell Events: 8123 mean_circ: 0.928002



End this measurment..



Stopping time: 1.17146e+008
****************************************
Measurement finished

Mean Circularitiy: 0.927984
Mean Area: 637.068
Mean FL-1 intensity: 3158.63
Mean FL-2 intensity: 4982.17
Mean FL-3 intensity: 0

frames processed: 156504
Total Time: 79247.3ms
Number of Runs (new bg img) 0
Frames collected: 158351
Skipped frames: 69819
Rescued Frames: 3404886
Samples DAQ: 39587761
Samples written: 79175378
Samples per Channel: 39587689
FPS: 1998.19

Samples per second: 499546
Expected samples/s: 500000

Events: 8136
Events per second: 102.666
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :237545
# 1 frames :66585
# 2 frames :3020
# 3 frames :1015
# 4 frames :543
# 5 frames :250
# 6 frames :2089
# 7 frames :1199
# 8 frames :1065
# 9 frames :591
# 10 frames :564
# 11 frames :326
# 12 frames :302
# 13 frames :190
# 14 frames :228
# 15 frames :172
# 16 frames :162
# 17 frames :137
# 18 frames :110
# 19 frames :75
# 20 frames :65
# 21 frames :50
# 22 frames :51
# 23 frames :44
# 24 frames :34
# 25 frames :33
# 26 frames :32
# 27 frames :18
# 28 frames :16
# 29 frames :17
# 30 frames :18
# 31 frames :15
# 32 frames :10
# 33 frames :7
# 34 frames :10
# 35 frames :9
# 36 frames :6
# 37 frames :5
# 38 frames :8
# 39 frames :7
# 40 frames :8
# 41 frames :2
# 42 frames :5
# 43 frames :5
# 44 frames :4
# 45 frames :2
# 46 frames :6
# 47 frames :4
# 48 frames :1
# 50 frames :1
# 51 frames :3
# 52 frames :1
# 53 frames :2
# 54 frames :1
# 56 frames :2
# 57 frames :1
# 59 frames :1
# 60 frames :1
# 63 frames :1
# 73 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.9499
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0