Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "9915bf47eb54f4790b5838b0676f2806",
"name": "M1_20170516_1821_dsRNA_G8_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:48:21"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8116 (max 8117) in '8116'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.0038
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.22355e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 23 mean_circ: 0.938173
Ran for 3050 frames Cell Events: 141 mean_circ: 0.928085
Ran for 3550 frames Cell Events: 160 mean_circ: 0.925408
Ran for 4550 frames Cell Events: 212 mean_circ: 0.927335
Ran for 6050 frames Cell Events: 324 mean_circ: 0.931197
Ran for 7550 frames Cell Events: 422 mean_circ: 0.932332
Ran for 8050 frames Cell Events: 443 mean_circ: 0.93265
Ran for 9050 frames Cell Events: 490 mean_circ: 0.932723
Ran for 9550 frames Cell Events: 518 mean_circ: 0.932928
Ran for 10550 frames Cell Events: 572 mean_circ: 0.933416
Ran for 11550 frames Cell Events: 614 mean_circ: 0.933115
Ran for 12550 frames Cell Events: 687 mean_circ: 0.933647
Ran for 13050 frames Cell Events: 714 mean_circ: 0.933913
Ran for 14550 frames Cell Events: 794 mean_circ: 0.933701
Ran for 15550 frames Cell Events: 854 mean_circ: 0.932517
Ran for 16050 frames Cell Events: 897 mean_circ: 0.932536
Ran for 16550 frames Cell Events: 912 mean_circ: 0.932714
Ran for 17050 frames Cell Events: 943 mean_circ: 0.932666
Ran for 17550 frames Cell Events: 953 mean_circ: 0.932708
Ran for 18050 frames Cell Events: 979 mean_circ: 0.932663
Ran for 18550 frames Cell Events: 1006 mean_circ: 0.932528
Ran for 19050 frames Cell Events: 1036 mean_circ: 0.932377
Ran for 19550 frames Cell Events: 1067 mean_circ: 0.932617
Ran for 20050 frames Cell Events: 1098 mean_circ: 0.932907
Ran for 22550 frames Cell Events: 1247 mean_circ: 0.932567
Ran for 25050 frames Cell Events: 1388 mean_circ: 0.93176
Ran for 25550 frames Cell Events: 1421 mean_circ: 0.931741
Ran for 26050 frames Cell Events: 1445 mean_circ: 0.931916
Ran for 27050 frames Cell Events: 1513 mean_circ: 0.931616
Ran for 27550 frames Cell Events: 1534 mean_circ: 0.931723
Ran for 28050 frames Cell Events: 1560 mean_circ: 0.931706
Ran for 28550 frames Cell Events: 1584 mean_circ: 0.931589
Ran for 29050 frames Cell Events: 1606 mean_circ: 0.931548
Ran for 30050 frames Cell Events: 1661 mean_circ: 0.931585
Ran for 30550 frames Cell Events: 1691 mean_circ: 0.931788
Ran for 31050 frames Cell Events: 1734 mean_circ: 0.931854
Ran for 32050 frames Cell Events: 1788 mean_circ: 0.932018
Ran for 32550 frames Cell Events: 1810 mean_circ: 0.932107
Ran for 33050 frames Cell Events: 1829 mean_circ: 0.932216
Ran for 34050 frames Cell Events: 1892 mean_circ: 0.932147
Ran for 34550 frames Cell Events: 1910 mean_circ: 0.932271
Ran for 35050 frames Cell Events: 1935 mean_circ: 0.932292
Ran for 36050 frames Cell Events: 2003 mean_circ: 0.93235
Ran for 37050 frames Cell Events: 2057 mean_circ: 0.932626
Ran for 38550 frames Cell Events: 2130 mean_circ: 0.93263
Ran for 39050 frames Cell Events: 2150 mean_circ: 0.932593
Ran for 39550 frames Cell Events: 2179 mean_circ: 0.932388
Ran for 41050 frames Cell Events: 2276 mean_circ: 0.93204
Ran for 41550 frames Cell Events: 2297 mean_circ: 0.931986
Ran for 42050 frames Cell Events: 2323 mean_circ: 0.932053
Ran for 43050 frames Cell Events: 2369 mean_circ: 0.932153
Ran for 44050 frames Cell Events: 2439 mean_circ: 0.932308
Ran for 45550 frames Cell Events: 2534 mean_circ: 0.932145
Ran for 46550 frames Cell Events: 2574 mean_circ: 0.932261
Ran for 48050 frames Cell Events: 2668 mean_circ: 0.931924
Ran for 49550 frames Cell Events: 2763 mean_circ: 0.932047
Ran for 51550 frames Cell Events: 2865 mean_circ: 0.932363
Ran for 52050 frames Cell Events: 2878 mean_circ: 0.93234
Ran for 53050 frames Cell Events: 2945 mean_circ: 0.932266
Ran for 53550 frames Cell Events: 2969 mean_circ: 0.932453
Ran for 54050 frames Cell Events: 2989 mean_circ: 0.932455
Ran for 54550 frames Cell Events: 2998 mean_circ: 0.93251
Ran for 55050 frames Cell Events: 3015 mean_circ: 0.932477
Ran for 55550 frames Cell Events: 3031 mean_circ: 0.932469
Ran for 56050 frames Cell Events: 3056 mean_circ: 0.932501
Ran for 57050 frames Cell Events: 3125 mean_circ: 0.932516
Ran for 57550 frames Cell Events: 3147 mean_circ: 0.932468
Ran for 58050 frames Cell Events: 3167 mean_circ: 0.932154
Ran for 59550 frames Cell Events: 3248 mean_circ: 0.932206
Ran for 61050 frames Cell Events: 3340 mean_circ: 0.93238
Ran for 61550 frames Cell Events: 3372 mean_circ: 0.932311
Ran for 62050 frames Cell Events: 3394 mean_circ: 0.93239
Ran for 63050 frames Cell Events: 3452 mean_circ: 0.932349
Ran for 63550 frames Cell Events: 3485 mean_circ: 0.932294
Ran for 64050 frames Cell Events: 3506 mean_circ: 0.932316
Ran for 64550 frames Cell Events: 3536 mean_circ: 0.932402
Ran for 65550 frames Cell Events: 3585 mean_circ: 0.932223
Ran for 66050 frames Cell Events: 3607 mean_circ: 0.932254
Ran for 66550 frames Cell Events: 3634 mean_circ: 0.932274
Ran for 68050 frames Cell Events: 3727 mean_circ: 0.932217
Ran for 68550 frames Cell Events: 3765 mean_circ: 0.932235
Ran for 69550 frames Cell Events: 3827 mean_circ: 0.932259
Ran for 70050 frames Cell Events: 3852 mean_circ: 0.932205
Ran for 71050 frames Cell Events: 3870 mean_circ: 0.932261
Ran for 73550 frames Cell Events: 4016 mean_circ: 0.932208
Ran for 74050 frames Cell Events: 4045 mean_circ: 0.932217
Ran for 74550 frames Cell Events: 4068 mean_circ: 0.932201
Ran for 75550 frames Cell Events: 4118 mean_circ: 0.932231
Ran for 76050 frames Cell Events: 4153 mean_circ: 0.932226
Ran for 77050 frames Cell Events: 4192 mean_circ: 0.932228
Ran for 77550 frames Cell Events: 4211 mean_circ: 0.932219
Ran for 78550 frames Cell Events: 4256 mean_circ: 0.932222
Ran for 79050 frames Cell Events: 4272 mean_circ: 0.932212
Ran for 80050 frames Cell Events: 4318 mean_circ: 0.93208
Ran for 80550 frames Cell Events: 4342 mean_circ: 0.932109
Ran for 81550 frames Cell Events: 4373 mean_circ: 0.932226
Ran for 82050 frames Cell Events: 4396 mean_circ: 0.932264
Ran for 83050 frames Cell Events: 4453 mean_circ: 0.932347
Ran for 83550 frames Cell Events: 4490 mean_circ: 0.932288
Ran for 84050 frames Cell Events: 4515 mean_circ: 0.932275
Ran for 84550 frames Cell Events: 4544 mean_circ: 0.932059
Ran for 85050 frames Cell Events: 4579 mean_circ: 0.932057
Ran for 87050 frames Cell Events: 4665 mean_circ: 0.93204
Ran for 87550 frames Cell Events: 4691 mean_circ: 0.932102
Ran for 90550 frames Cell Events: 4850 mean_circ: 0.931975
Ran for 91050 frames Cell Events: 4882 mean_circ: 0.931964
Ran for 91550 frames Cell Events: 4914 mean_circ: 0.931957
Ran for 93050 frames Cell Events: 4988 mean_circ: 0.931951
Ran for 93550 frames Cell Events: 5008 mean_circ: 0.931879
Ran for 94050 frames Cell Events: 5025 mean_circ: 0.931828
Ran for 95050 frames Cell Events: 5091 mean_circ: 0.931864
Ran for 95550 frames Cell Events: 5118 mean_circ: 0.931898
Ran for 96050 frames Cell Events: 5140 mean_circ: 0.931921
Ran for 96550 frames Cell Events: 5183 mean_circ: 0.931971
Ran for 97050 frames Cell Events: 5216 mean_circ: 0.931899
Ran for 97550 frames Cell Events: 5239 mean_circ: 0.931836
Ran for 98050 frames Cell Events: 5263 mean_circ: 0.931837
Ran for 98550 frames Cell Events: 5285 mean_circ: 0.931855
Ran for 99050 frames Cell Events: 5310 mean_circ: 0.931876
Ran for 99550 frames Cell Events: 5332 mean_circ: 0.931832
Ran for 100050 frames Cell Events: 5353 mean_circ: 0.93182
Ran for 100550 frames Cell Events: 5369 mean_circ: 0.931814
Ran for 101050 frames Cell Events: 5399 mean_circ: 0.9318
Ran for 101550 frames Cell Events: 5414 mean_circ: 0.931835
Ran for 103550 frames Cell Events: 5516 mean_circ: 0.931738
Ran for 104050 frames Cell Events: 5538 mean_circ: 0.931735
Ran for 105550 frames Cell Events: 5616 mean_circ: 0.931821
Ran for 106050 frames Cell Events: 5645 mean_circ: 0.931848
Ran for 106550 frames Cell Events: 5669 mean_circ: 0.931839
Ran for 107550 frames Cell Events: 5728 mean_circ: 0.931816
Ran for 108050 frames Cell Events: 5764 mean_circ: 0.93171
Ran for 109050 frames Cell Events: 5822 mean_circ: 0.931718
Ran for 109550 frames Cell Events: 5855 mean_circ: 0.931611
Ran for 110550 frames Cell Events: 5881 mean_circ: 0.931583
Ran for 111050 frames Cell Events: 5899 mean_circ: 0.931591
Ran for 112550 frames Cell Events: 5978 mean_circ: 0.931617
Ran for 113050 frames Cell Events: 6014 mean_circ: 0.931601
Ran for 114550 frames Cell Events: 6088 mean_circ: 0.931721
Ran for 115050 frames Cell Events: 6115 mean_circ: 0.931789
Ran for 115550 frames Cell Events: 6147 mean_circ: 0.931831
Ran for 116050 frames Cell Events: 6176 mean_circ: 0.931797
Ran for 116550 frames Cell Events: 6205 mean_circ: 0.931741
Ran for 117550 frames Cell Events: 6288 mean_circ: 0.931784
Ran for 119050 frames Cell Events: 6368 mean_circ: 0.931797
Ran for 120050 frames Cell Events: 6434 mean_circ: 0.931893
Ran for 121050 frames Cell Events: 6496 mean_circ: 0.931921
Ran for 121550 frames Cell Events: 6526 mean_circ: 0.931975
Ran for 122050 frames Cell Events: 6549 mean_circ: 0.931977
Ran for 122550 frames Cell Events: 6568 mean_circ: 0.931981
Ran for 123550 frames Cell Events: 6625 mean_circ: 0.931909
Ran for 124050 frames Cell Events: 6651 mean_circ: 0.931948
Ran for 124550 frames Cell Events: 6678 mean_circ: 0.931969
Ran for 125050 frames Cell Events: 6692 mean_circ: 0.931945
Ran for 125550 frames Cell Events: 6711 mean_circ: 0.931927
Ran for 126050 frames Cell Events: 6737 mean_circ: 0.931924
Ran for 126550 frames Cell Events: 6774 mean_circ: 0.931872
Ran for 127050 frames Cell Events: 6803 mean_circ: 0.931898
Ran for 127550 frames Cell Events: 6835 mean_circ: 0.931855
Ran for 128050 frames Cell Events: 6871 mean_circ: 0.931848
Ran for 129550 frames Cell Events: 6950 mean_circ: 0.9318
Ran for 130050 frames Cell Events: 6977 mean_circ: 0.931673
Ran for 130550 frames Cell Events: 6998 mean_circ: 0.931668
Ran for 131550 frames Cell Events: 7049 mean_circ: 0.931728
Ran for 132050 frames Cell Events: 7072 mean_circ: 0.931774
Ran for 132550 frames Cell Events: 7091 mean_circ: 0.931785
Ran for 133050 frames Cell Events: 7121 mean_circ: 0.931707
Ran for 133550 frames Cell Events: 7149 mean_circ: 0.931706
Ran for 134550 frames Cell Events: 7204 mean_circ: 0.931778
Ran for 135050 frames Cell Events: 7217 mean_circ: 0.931775
Ran for 135550 frames Cell Events: 7252 mean_circ: 0.931801
Ran for 136050 frames Cell Events: 7273 mean_circ: 0.931779
Ran for 136550 frames Cell Events: 7295 mean_circ: 0.931721
Ran for 137050 frames Cell Events: 7312 mean_circ: 0.931705
Ran for 137550 frames Cell Events: 7327 mean_circ: 0.931727
Ran for 138550 frames Cell Events: 7364 mean_circ: 0.931735
Ran for 139050 frames Cell Events: 7389 mean_circ: 0.931768
Ran for 139550 frames Cell Events: 7423 mean_circ: 0.931786
Ran for 140050 frames Cell Events: 7447 mean_circ: 0.931743
Ran for 140550 frames Cell Events: 7475 mean_circ: 0.931752
Ran for 141050 frames Cell Events: 7497 mean_circ: 0.931718
Ran for 141550 frames Cell Events: 7533 mean_circ: 0.931709
Ran for 142050 frames Cell Events: 7557 mean_circ: 0.931683
Ran for 143050 frames Cell Events: 7599 mean_circ: 0.931644
Ran for 143550 frames Cell Events: 7629 mean_circ: 0.931659
Ran for 145550 frames Cell Events: 7734 mean_circ: 0.931598
Ran for 146550 frames Cell Events: 7771 mean_circ: 0.931594
Ran for 147050 frames Cell Events: 7796 mean_circ: 0.931557
Ran for 148050 frames Cell Events: 7863 mean_circ: 0.931533
Ran for 149050 frames Cell Events: 7925 mean_circ: 0.931555
Ran for 150050 frames Cell Events: 7980 mean_circ: 0.931488
Ran for 151050 frames Cell Events: 8026 mean_circ: 0.931503
Ran for 152050 frames Cell Events: 8073 mean_circ: 0.931525



End this measurment..



Stopping time: 1.22431e+008
****************************************
Measurement finished

Mean Circularitiy: 0.931552
Mean Area: 646.879
Mean FL-1 intensity: 3126.29
Mean FL-2 intensity: 4898.75
Mean FL-3 intensity: 0

frames processed: 151437
Total Time: 76590.2ms
Number of Runs (new bg img) 0
Frames collected: 153016
Skipped frames: 2753
Rescued Frames: 279239
Samples DAQ: 38256183
Samples written: 76507724
Samples per Channel: 38253862
FPS: 1997.85

Samples per second: 499462
Expected samples/s: 500000

Events: 8117
Events per second: 105.98
jumped back (PMT): 13
#Frames read, frequency
# 0 frames :213483
# 1 frames :80195
# 2 frames :2651
# 3 frames :1023
# 4 frames :299
# 5 frames :3197
# 6 frames :1635
# 7 frames :1000
# 8 frames :524
# 9 frames :269
# 10 frames :415
# 11 frames :363
# 12 frames :240
# 13 frames :149
# 14 frames :126
# 15 frames :86
# 16 frames :79
# 17 frames :58
# 18 frames :37
# 19 frames :37
# 20 frames :23
# 21 frames :17
# 22 frames :15
# 23 frames :14
# 24 frames :10
# 25 frames :7
# 26 frames :5
# 27 frames :4
# 28 frames :5
# 29 frames :2
# 31 frames :2
# 32 frames :1
# 33 frames :1
# 34 frames :1
# 37 frames :2
# 38 frames :1
# 41 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.0038
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0