Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2faba1a315ed9dd3788f9bdadcbef3b4",
"name": "M1_20170516_1846_dsRNA_D7_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:50:25"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.9672
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.23828e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0
Ran for 550 frames Cell Events: 16 mean_circ: 0.930292
Ran for 3550 frames Cell Events: 168 mean_circ: 0.937981
Ran for 4550 frames Cell Events: 214 mean_circ: 0.938462
Ran for 5550 frames Cell Events: 261 mean_circ: 0.935868
Ran for 7050 frames Cell Events: 363 mean_circ: 0.936277
Ran for 9050 frames Cell Events: 461 mean_circ: 0.936621
Ran for 9550 frames Cell Events: 480 mean_circ: 0.936582
Ran for 11550 frames Cell Events: 599 mean_circ: 0.936068
Ran for 12050 frames Cell Events: 632 mean_circ: 0.936043
Ran for 12550 frames Cell Events: 655 mean_circ: 0.936135
Ran for 13050 frames Cell Events: 675 mean_circ: 0.936591
Ran for 13550 frames Cell Events: 695 mean_circ: 0.936817
Ran for 16050 frames Cell Events: 873 mean_circ: 0.936102
Ran for 17550 frames Cell Events: 958 mean_circ: 0.9347
Ran for 18050 frames Cell Events: 979 mean_circ: 0.934564
Ran for 18550 frames Cell Events: 1000 mean_circ: 0.934663
Ran for 19050 frames Cell Events: 1024 mean_circ: 0.934735
Ran for 20550 frames Cell Events: 1118 mean_circ: 0.934447
Ran for 21050 frames Cell Events: 1142 mean_circ: 0.934489
Ran for 21550 frames Cell Events: 1164 mean_circ: 0.934281
Ran for 22050 frames Cell Events: 1191 mean_circ: 0.934551
Ran for 23550 frames Cell Events: 1259 mean_circ: 0.934189
Ran for 24050 frames Cell Events: 1280 mean_circ: 0.933923
Ran for 24550 frames Cell Events: 1293 mean_circ: 0.934063
Ran for 25050 frames Cell Events: 1321 mean_circ: 0.933882
Ran for 25550 frames Cell Events: 1357 mean_circ: 0.933444
Ran for 26050 frames Cell Events: 1372 mean_circ: 0.933391
Ran for 26550 frames Cell Events: 1408 mean_circ: 0.933496
Ran for 27550 frames Cell Events: 1453 mean_circ: 0.933684
Ran for 28050 frames Cell Events: 1478 mean_circ: 0.933562
Ran for 28550 frames Cell Events: 1502 mean_circ: 0.933607
Ran for 30050 frames Cell Events: 1597 mean_circ: 0.933643
Ran for 30550 frames Cell Events: 1609 mean_circ: 0.933759
Ran for 31050 frames Cell Events: 1639 mean_circ: 0.934016
Ran for 31550 frames Cell Events: 1670 mean_circ: 0.934412
Ran for 32050 frames Cell Events: 1690 mean_circ: 0.934408
Ran for 33550 frames Cell Events: 1768 mean_circ: 0.934399
Ran for 34050 frames Cell Events: 1793 mean_circ: 0.934436
Ran for 34550 frames Cell Events: 1825 mean_circ: 0.934507
Ran for 35550 frames Cell Events: 1896 mean_circ: 0.934599
Ran for 36050 frames Cell Events: 1922 mean_circ: 0.934684
Ran for 36550 frames Cell Events: 1945 mean_circ: 0.934568
Ran for 37550 frames Cell Events: 2000 mean_circ: 0.934843
Ran for 38550 frames Cell Events: 2061 mean_circ: 0.93474
Ran for 39550 frames Cell Events: 2107 mean_circ: 0.934795
Ran for 40050 frames Cell Events: 2132 mean_circ: 0.934661
Ran for 41050 frames Cell Events: 2178 mean_circ: 0.934543
Ran for 41550 frames Cell Events: 2202 mean_circ: 0.934487
Ran for 42050 frames Cell Events: 2225 mean_circ: 0.93441
Ran for 42550 frames Cell Events: 2252 mean_circ: 0.9344
Ran for 43050 frames Cell Events: 2284 mean_circ: 0.934416
Ran for 44050 frames Cell Events: 2319 mean_circ: 0.934473
Ran for 45050 frames Cell Events: 2387 mean_circ: 0.934794
Ran for 45550 frames Cell Events: 2408 mean_circ: 0.934683
Ran for 46550 frames Cell Events: 2464 mean_circ: 0.934584
Ran for 48550 frames Cell Events: 2561 mean_circ: 0.934892
Ran for 49050 frames Cell Events: 2578 mean_circ: 0.934911
Ran for 49550 frames Cell Events: 2608 mean_circ: 0.934908
Ran for 50050 frames Cell Events: 2634 mean_circ: 0.934998
Ran for 51050 frames Cell Events: 2684 mean_circ: 0.935236
Ran for 51550 frames Cell Events: 2706 mean_circ: 0.935107
Ran for 52550 frames Cell Events: 2756 mean_circ: 0.935202
Ran for 53050 frames Cell Events: 2779 mean_circ: 0.935299
Ran for 54550 frames Cell Events: 2864 mean_circ: 0.935132
Ran for 55050 frames Cell Events: 2887 mean_circ: 0.935194
Ran for 56050 frames Cell Events: 2951 mean_circ: 0.935363
Ran for 57550 frames Cell Events: 3027 mean_circ: 0.935364
Ran for 58050 frames Cell Events: 3051 mean_circ: 0.935424
Ran for 60050 frames Cell Events: 3174 mean_circ: 0.935615
Ran for 60550 frames Cell Events: 3196 mean_circ: 0.935664
Ran for 61550 frames Cell Events: 3265 mean_circ: 0.935541
Ran for 62050 frames Cell Events: 3287 mean_circ: 0.935525
Ran for 62550 frames Cell Events: 3306 mean_circ: 0.935566
Ran for 63050 frames Cell Events: 3331 mean_circ: 0.93553
Ran for 63550 frames Cell Events: 3356 mean_circ: 0.935554
Ran for 64050 frames Cell Events: 3387 mean_circ: 0.935459
Ran for 64550 frames Cell Events: 3406 mean_circ: 0.935452
Ran for 65050 frames Cell Events: 3432 mean_circ: 0.93543
Ran for 66550 frames Cell Events: 3522 mean_circ: 0.935355
Ran for 67050 frames Cell Events: 3550 mean_circ: 0.935496
Ran for 68050 frames Cell Events: 3598 mean_circ: 0.935558
Ran for 69050 frames Cell Events: 3649 mean_circ: 0.935558
Ran for 70050 frames Cell Events: 3718 mean_circ: 0.935473
Ran for 70550 frames Cell Events: 3749 mean_circ: 0.935583
Ran for 71550 frames Cell Events: 3805 mean_circ: 0.935514
Ran for 72550 frames Cell Events: 3846 mean_circ: 0.93554
Ran for 73050 frames Cell Events: 3880 mean_circ: 0.935529
Ran for 73550 frames Cell Events: 3914 mean_circ: 0.935413
Ran for 74050 frames Cell Events: 3939 mean_circ: 0.935432
Ran for 75050 frames Cell Events: 4005 mean_circ: 0.93542
Ran for 75550 frames Cell Events: 4032 mean_circ: 0.935378
Ran for 76550 frames Cell Events: 4096 mean_circ: 0.935413
Ran for 77050 frames Cell Events: 4112 mean_circ: 0.935482
Ran for 78050 frames Cell Events: 4161 mean_circ: 0.935523
Ran for 78550 frames Cell Events: 4180 mean_circ: 0.935561
Ran for 79050 frames Cell Events: 4203 mean_circ: 0.935532
Ran for 80050 frames Cell Events: 4256 mean_circ: 0.935663
Ran for 80550 frames Cell Events: 4269 mean_circ: 0.935642
Ran for 81550 frames Cell Events: 4323 mean_circ: 0.935517
Ran for 82050 frames Cell Events: 4346 mean_circ: 0.935553
Ran for 82550 frames Cell Events: 4372 mean_circ: 0.935478
Ran for 83050 frames Cell Events: 4406 mean_circ: 0.935476
Ran for 83550 frames Cell Events: 4429 mean_circ: 0.935499
Ran for 85050 frames Cell Events: 4520 mean_circ: 0.935576
Ran for 86050 frames Cell Events: 4582 mean_circ: 0.935563
Ran for 86550 frames Cell Events: 4602 mean_circ: 0.93552
Ran for 87550 frames Cell Events: 4651 mean_circ: 0.935457
Ran for 88550 frames Cell Events: 4727 mean_circ: 0.935608
Ran for 90050 frames Cell Events: 4802 mean_circ: 0.935583
Ran for 90550 frames Cell Events: 4827 mean_circ: 0.935526
Ran for 91550 frames Cell Events: 4884 mean_circ: 0.935474
Ran for 92050 frames Cell Events: 4911 mean_circ: 0.935533
Ran for 92550 frames Cell Events: 4936 mean_circ: 0.935527
Ran for 93050 frames Cell Events: 4969 mean_circ: 0.935487
Ran for 96050 frames Cell Events: 5129 mean_circ: 0.935344
Ran for 96550 frames Cell Events: 5150 mean_circ: 0.935358
Ran for 97050 frames Cell Events: 5170 mean_circ: 0.935335
Ran for 98050 frames Cell Events: 5223 mean_circ: 0.935317
Ran for 100550 frames Cell Events: 5329 mean_circ: 0.935213
Ran for 101550 frames Cell Events: 5376 mean_circ: 0.935208
Ran for 102050 frames Cell Events: 5392 mean_circ: 0.935177
Ran for 103550 frames Cell Events: 5473 mean_circ: 0.935191
Ran for 104550 frames Cell Events: 5516 mean_circ: 0.935231
Ran for 105050 frames Cell Events: 5551 mean_circ: 0.935236
Ran for 105550 frames Cell Events: 5565 mean_circ: 0.935194
Ran for 106050 frames Cell Events: 5578 mean_circ: 0.935153
Ran for 107050 frames Cell Events: 5622 mean_circ: 0.935216
Ran for 107550 frames Cell Events: 5650 mean_circ: 0.935194
Ran for 108550 frames Cell Events: 5714 mean_circ: 0.935227
Ran for 110050 frames Cell Events: 5798 mean_circ: 0.93518
Ran for 111050 frames Cell Events: 5860 mean_circ: 0.935244
Ran for 111550 frames Cell Events: 5880 mean_circ: 0.935225
Ran for 112550 frames Cell Events: 5929 mean_circ: 0.935225
Ran for 113050 frames Cell Events: 5947 mean_circ: 0.935197
Ran for 114050 frames Cell Events: 5993 mean_circ: 0.935198
Ran for 114550 frames Cell Events: 6012 mean_circ: 0.935244
Ran for 115550 frames Cell Events: 6064 mean_circ: 0.935224
Ran for 118050 frames Cell Events: 6220 mean_circ: 0.935163
Ran for 118550 frames Cell Events: 6253 mean_circ: 0.935144
Ran for 119050 frames Cell Events: 6277 mean_circ: 0.93519
Ran for 119550 frames Cell Events: 6296 mean_circ: 0.935203
Ran for 120050 frames Cell Events: 6328 mean_circ: 0.93512
Ran for 120550 frames Cell Events: 6362 mean_circ: 0.935168
Ran for 122050 frames Cell Events: 6439 mean_circ: 0.935152
Ran for 124050 frames Cell Events: 6574 mean_circ: 0.935109
Ran for 125550 frames Cell Events: 6672 mean_circ: 0.93514
Ran for 128550 frames Cell Events: 6839 mean_circ: 0.935216
Ran for 129050 frames Cell Events: 6868 mean_circ: 0.935265
Ran for 129550 frames Cell Events: 6896 mean_circ: 0.935245
Ran for 130050 frames Cell Events: 6923 mean_circ: 0.935163
Ran for 130550 frames Cell Events: 6938 mean_circ: 0.935177
Ran for 131550 frames Cell Events: 6985 mean_circ: 0.935264
Ran for 132550 frames Cell Events: 7034 mean_circ: 0.9353
Ran for 133550 frames Cell Events: 7096 mean_circ: 0.935364
Ran for 134050 frames Cell Events: 7118 mean_circ: 0.935399
Ran for 134550 frames Cell Events: 7140 mean_circ: 0.935304
Ran for 136550 frames Cell Events: 7272 mean_circ: 0.935231
Ran for 137050 frames Cell Events: 7299 mean_circ: 0.935213
Ran for 137550 frames Cell Events: 7323 mean_circ: 0.935235
Ran for 138050 frames Cell Events: 7345 mean_circ: 0.935268
Ran for 139550 frames Cell Events: 7431 mean_circ: 0.935254
Ran for 141050 frames Cell Events: 7513 mean_circ: 0.935263
Ran for 141550 frames Cell Events: 7547 mean_circ: 0.93523
Ran for 142050 frames Cell Events: 7583 mean_circ: 0.935167
Ran for 142550 frames Cell Events: 7617 mean_circ: 0.935188
Ran for 144050 frames Cell Events: 7709 mean_circ: 0.935249
Ran for 144550 frames Cell Events: 7738 mean_circ: 0.935253
Ran for 145050 frames Cell Events: 7762 mean_circ: 0.935293
Ran for 146050 frames Cell Events: 7813 mean_circ: 0.935276
Ran for 146550 frames Cell Events: 7840 mean_circ: 0.935222
Ran for 148050 frames Cell Events: 7937 mean_circ: 0.935276
Ran for 149050 frames Cell Events: 7971 mean_circ: 0.935247
Ran for 149550 frames Cell Events: 7990 mean_circ: 0.935227
Ran for 150550 frames Cell Events: 8030 mean_circ: 0.93522
Ran for 151050 frames Cell Events: 8055 mean_circ: 0.935189
Ran for 152050 frames Cell Events: 8108 mean_circ: 0.935156
Ran for 152550 frames Cell Events: 8124 mean_circ: 0.935163
Ran for 153050 frames Cell Events: 8143 mean_circ: 0.935151
Ran for 153550 frames Cell Events: 8182 mean_circ: 0.935154
Ran for 155050 frames Cell Events: 8284 mean_circ: 0.935146
Ran for 155550 frames Cell Events: 8314 mean_circ: 0.935134



End this measurment..



Stopping time: 1.23906e+008
****************************************
Measurement finished

Mean Circularitiy: 0.935113
Mean Area: 625.703
Mean FL-1 intensity: 3428.13
Mean FL-2 intensity: 4861.46
Mean FL-3 intensity: 0

frames processed: 155266
Total Time: 78050.1ms
Number of Runs (new bg img) 0
Frames collected: 155933
Skipped frames: 667
Rescued Frames: 81036
Samples DAQ: 38983177
Samples written: 77966234
Samples per Channel: 38983117
FPS: 1997.86

Samples per second: 499463
Expected samples/s: 500000

Events: 8339
Events per second: 106.842
jumped back (PMT): 15
#Frames read, frequency
# 0 frames :237505
# 1 frames :60989
# 2 frames :3164
# 3 frames :1077
# 4 frames :711
# 5 frames :741
# 6 frames :1823
# 7 frames :1161
# 8 frames :980
# 9 frames :627
# 10 frames :454
# 11 frames :369
# 12 frames :273
# 13 frames :274
# 14 frames :312
# 15 frames :266
# 16 frames :197
# 17 frames :128
# 18 frames :106
# 19 frames :103
# 20 frames :67
# 21 frames :58
# 22 frames :46
# 23 frames :33
# 24 frames :33
# 25 frames :44
# 26 frames :24
# 27 frames :23
# 28 frames :11
# 29 frames :12
# 30 frames :12
# 31 frames :17
# 32 frames :9
# 33 frames :12
# 34 frames :12
# 35 frames :6
# 36 frames :8
# 37 frames :5
# 38 frames :5
# 39 frames :4
# 40 frames :4
# 41 frames :4
# 42 frames :4
# 43 frames :4
# 44 frames :1
# 45 frames :3
# 46 frames :6
# 47 frames :3
# 48 frames :3
# 49 frames :1
# 50 frames :1
# 51 frames :2
# 53 frames :2
# 54 frames :2
# 57 frames :1
# 58 frames :1
# 61 frames :1
# 81 frames :1
# 94 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.9672
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0