Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c3e515c12730ddc6f92fe3f56af31c5d",
"name": "M1_20170518_1403_dsRNA_H2_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:33:55"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8341 (max 8342) in '8341'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.8907
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.79641e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 29 mean_circ: 0.93471
Ran for 1550 frames Cell Events: 74 mean_circ: 0.942463
Ran for 3050 frames Cell Events: 150 mean_circ: 0.942835
Ran for 3550 frames Cell Events: 173 mean_circ: 0.944007
Ran for 4050 frames Cell Events: 193 mean_circ: 0.944916
Ran for 4550 frames Cell Events: 223 mean_circ: 0.944159
Ran for 5050 frames Cell Events: 243 mean_circ: 0.945028
Ran for 6550 frames Cell Events: 298 mean_circ: 0.943305
Ran for 7550 frames Cell Events: 364 mean_circ: 0.943587
Ran for 8050 frames Cell Events: 385 mean_circ: 0.944052
Ran for 8550 frames Cell Events: 415 mean_circ: 0.944979
Ran for 9050 frames Cell Events: 451 mean_circ: 0.94602
Ran for 9550 frames Cell Events: 470 mean_circ: 0.945677
Ran for 11050 frames Cell Events: 556 mean_circ: 0.946426
Ran for 11550 frames Cell Events: 577 mean_circ: 0.946562
Ran for 12050 frames Cell Events: 601 mean_circ: 0.94651
Ran for 12550 frames Cell Events: 631 mean_circ: 0.94634
Ran for 13050 frames Cell Events: 650 mean_circ: 0.946746
Ran for 15050 frames Cell Events: 773 mean_circ: 0.946065
Ran for 15550 frames Cell Events: 805 mean_circ: 0.945902
Ran for 16050 frames Cell Events: 835 mean_circ: 0.945727
Ran for 16550 frames Cell Events: 864 mean_circ: 0.94591
Ran for 17050 frames Cell Events: 892 mean_circ: 0.946061
Ran for 18050 frames Cell Events: 936 mean_circ: 0.945751
Ran for 19050 frames Cell Events: 1002 mean_circ: 0.945575
Ran for 21050 frames Cell Events: 1133 mean_circ: 0.944507
Ran for 21550 frames Cell Events: 1174 mean_circ: 0.944697
Ran for 23550 frames Cell Events: 1353 mean_circ: 0.944034
Ran for 24050 frames Cell Events: 1376 mean_circ: 0.944069
Ran for 25050 frames Cell Events: 1429 mean_circ: 0.943888
Ran for 26050 frames Cell Events: 1484 mean_circ: 0.943449
Ran for 27050 frames Cell Events: 1565 mean_circ: 0.943062
Ran for 27550 frames Cell Events: 1595 mean_circ: 0.943056
Ran for 29550 frames Cell Events: 1759 mean_circ: 0.943449
Ran for 30050 frames Cell Events: 1795 mean_circ: 0.943343
Ran for 30550 frames Cell Events: 1821 mean_circ: 0.943244
Ran for 31050 frames Cell Events: 1844 mean_circ: 0.943064
Ran for 31550 frames Cell Events: 1879 mean_circ: 0.942951
Ran for 32050 frames Cell Events: 1906 mean_circ: 0.943092
Ran for 32550 frames Cell Events: 1939 mean_circ: 0.94326
Ran for 34550 frames Cell Events: 2078 mean_circ: 0.94309
Ran for 35050 frames Cell Events: 2101 mean_circ: 0.943104
Ran for 35550 frames Cell Events: 2136 mean_circ: 0.943111
Ran for 36550 frames Cell Events: 2208 mean_circ: 0.943042
Ran for 37050 frames Cell Events: 2232 mean_circ: 0.943096
Ran for 38050 frames Cell Events: 2275 mean_circ: 0.943335
Ran for 39050 frames Cell Events: 2340 mean_circ: 0.943306
Ran for 40050 frames Cell Events: 2379 mean_circ: 0.943292
Ran for 40550 frames Cell Events: 2408 mean_circ: 0.943158
Ran for 41050 frames Cell Events: 2440 mean_circ: 0.943157
Ran for 42050 frames Cell Events: 2494 mean_circ: 0.943404
Ran for 42550 frames Cell Events: 2529 mean_circ: 0.943345
Ran for 45050 frames Cell Events: 2645 mean_circ: 0.943507
Ran for 45550 frames Cell Events: 2682 mean_circ: 0.943507
Ran for 46050 frames Cell Events: 2714 mean_circ: 0.943399
Ran for 47050 frames Cell Events: 2779 mean_circ: 0.943307
Ran for 48550 frames Cell Events: 2882 mean_circ: 0.943294
Ran for 49050 frames Cell Events: 2904 mean_circ: 0.943295
Ran for 49550 frames Cell Events: 2929 mean_circ: 0.943316
Ran for 50050 frames Cell Events: 2953 mean_circ: 0.943371
Ran for 50550 frames Cell Events: 2979 mean_circ: 0.943431
Ran for 51050 frames Cell Events: 3008 mean_circ: 0.943562
Ran for 52050 frames Cell Events: 3061 mean_circ: 0.943674
Ran for 52550 frames Cell Events: 3082 mean_circ: 0.943583
Ran for 53050 frames Cell Events: 3108 mean_circ: 0.943566
Ran for 54050 frames Cell Events: 3171 mean_circ: 0.94376
Ran for 55050 frames Cell Events: 3224 mean_circ: 0.943707
Ran for 55550 frames Cell Events: 3252 mean_circ: 0.943758
Ran for 56050 frames Cell Events: 3268 mean_circ: 0.943828
Ran for 56550 frames Cell Events: 3284 mean_circ: 0.943914
Ran for 57050 frames Cell Events: 3317 mean_circ: 0.943982
Ran for 57550 frames Cell Events: 3340 mean_circ: 0.943965
Ran for 59050 frames Cell Events: 3423 mean_circ: 0.943932
Ran for 59550 frames Cell Events: 3448 mean_circ: 0.943956
Ran for 60550 frames Cell Events: 3514 mean_circ: 0.943887
Ran for 61050 frames Cell Events: 3546 mean_circ: 0.943835
Ran for 61550 frames Cell Events: 3581 mean_circ: 0.943799
Ran for 62550 frames Cell Events: 3642 mean_circ: 0.943804
Ran for 63050 frames Cell Events: 3665 mean_circ: 0.943804
Ran for 64050 frames Cell Events: 3713 mean_circ: 0.943894
Ran for 65050 frames Cell Events: 3787 mean_circ: 0.943954
Ran for 66550 frames Cell Events: 3891 mean_circ: 0.94398
Ran for 67050 frames Cell Events: 3921 mean_circ: 0.944019
Ran for 67550 frames Cell Events: 3949 mean_circ: 0.944046
Ran for 68050 frames Cell Events: 3972 mean_circ: 0.944048
Ran for 68550 frames Cell Events: 4008 mean_circ: 0.94406
Ran for 69050 frames Cell Events: 4028 mean_circ: 0.943974
Ran for 70050 frames Cell Events: 4080 mean_circ: 0.943949
Ran for 70550 frames Cell Events: 4101 mean_circ: 0.94403
Ran for 71050 frames Cell Events: 4129 mean_circ: 0.944016
Ran for 71550 frames Cell Events: 4172 mean_circ: 0.944015
Ran for 72550 frames Cell Events: 4239 mean_circ: 0.943892
Ran for 73050 frames Cell Events: 4266 mean_circ: 0.943906
Ran for 73550 frames Cell Events: 4298 mean_circ: 0.943853
Ran for 74050 frames Cell Events: 4331 mean_circ: 0.943829
Ran for 74550 frames Cell Events: 4356 mean_circ: 0.943862
Ran for 76550 frames Cell Events: 4503 mean_circ: 0.943853
Ran for 77550 frames Cell Events: 4559 mean_circ: 0.943808
Ran for 78050 frames Cell Events: 4590 mean_circ: 0.943873
Ran for 78550 frames Cell Events: 4623 mean_circ: 0.943883
Ran for 79550 frames Cell Events: 4685 mean_circ: 0.943969
Ran for 80050 frames Cell Events: 4720 mean_circ: 0.943961
Ran for 82050 frames Cell Events: 4854 mean_circ: 0.943938
Ran for 85550 frames Cell Events: 5065 mean_circ: 0.943943
Ran for 86550 frames Cell Events: 5117 mean_circ: 0.943977
Ran for 87050 frames Cell Events: 5132 mean_circ: 0.944002
Ran for 87550 frames Cell Events: 5171 mean_circ: 0.943947
Ran for 88050 frames Cell Events: 5199 mean_circ: 0.944
Ran for 88550 frames Cell Events: 5236 mean_circ: 0.943935
Ran for 89550 frames Cell Events: 5282 mean_circ: 0.943905
Ran for 90550 frames Cell Events: 5326 mean_circ: 0.943889
Ran for 91550 frames Cell Events: 5377 mean_circ: 0.943842
Ran for 95050 frames Cell Events: 5621 mean_circ: 0.943977
Ran for 96050 frames Cell Events: 5658 mean_circ: 0.943977
Ran for 96550 frames Cell Events: 5693 mean_circ: 0.94396
Ran for 97050 frames Cell Events: 5728 mean_circ: 0.943942
Ran for 97550 frames Cell Events: 5754 mean_circ: 0.943884
Ran for 99050 frames Cell Events: 5853 mean_circ: 0.943769
Ran for 101550 frames Cell Events: 6004 mean_circ: 0.943701
Ran for 103550 frames Cell Events: 6102 mean_circ: 0.943608
Ran for 105050 frames Cell Events: 6209 mean_circ: 0.943661
Ran for 106550 frames Cell Events: 6294 mean_circ: 0.943701
Ran for 108050 frames Cell Events: 6384 mean_circ: 0.943683
Ran for 108550 frames Cell Events: 6421 mean_circ: 0.94362
Ran for 110550 frames Cell Events: 6555 mean_circ: 0.943674
Ran for 111550 frames Cell Events: 6614 mean_circ: 0.94362
Ran for 112050 frames Cell Events: 6639 mean_circ: 0.94357
Ran for 113050 frames Cell Events: 6697 mean_circ: 0.943666
Ran for 114050 frames Cell Events: 6746 mean_circ: 0.943623
Ran for 114550 frames Cell Events: 6778 mean_circ: 0.943657
Ran for 115550 frames Cell Events: 6842 mean_circ: 0.943669
Ran for 117050 frames Cell Events: 6918 mean_circ: 0.943653
Ran for 117550 frames Cell Events: 6946 mean_circ: 0.943645
Ran for 118550 frames Cell Events: 7011 mean_circ: 0.943574
Ran for 119050 frames Cell Events: 7043 mean_circ: 0.943511
Ran for 119550 frames Cell Events: 7078 mean_circ: 0.943498
Ran for 120050 frames Cell Events: 7106 mean_circ: 0.943513
Ran for 120550 frames Cell Events: 7132 mean_circ: 0.943532
Ran for 121550 frames Cell Events: 7201 mean_circ: 0.943569
Ran for 123050 frames Cell Events: 7302 mean_circ: 0.943461
Ran for 124050 frames Cell Events: 7360 mean_circ: 0.943426
Ran for 126050 frames Cell Events: 7468 mean_circ: 0.943348
Ran for 126550 frames Cell Events: 7494 mean_circ: 0.943369
Ran for 128050 frames Cell Events: 7607 mean_circ: 0.943329
Ran for 128550 frames Cell Events: 7640 mean_circ: 0.943396
Ran for 129550 frames Cell Events: 7686 mean_circ: 0.943393
Ran for 130550 frames Cell Events: 7748 mean_circ: 0.943428
Ran for 132050 frames Cell Events: 7832 mean_circ: 0.943369
Ran for 132550 frames Cell Events: 7872 mean_circ: 0.943374
Ran for 133050 frames Cell Events: 7916 mean_circ: 0.943374
Ran for 133550 frames Cell Events: 7946 mean_circ: 0.943406
Ran for 135550 frames Cell Events: 8062 mean_circ: 0.943471
Ran for 136050 frames Cell Events: 8090 mean_circ: 0.943435
Ran for 137550 frames Cell Events: 8177 mean_circ: 0.943447



End this measurment..



Stopping time: 2.79711e+008
****************************************
Measurement finished

Mean Circularitiy: 0.943474
Mean Area: 618.037
Mean FL-1 intensity: 3164.8
Mean FL-2 intensity: 4888.61
Mean FL-3 intensity: 0

frames processed: 138531
Total Time: 70107.7ms
Number of Runs (new bg img) 0
Frames collected: 140038
Skipped frames: 19952
Rescued Frames: 1215352
Samples DAQ: 35011004
Samples written: 70019160
Samples per Channel: 35009580
FPS: 1997.47

Samples per second: 499369
Expected samples/s: 500000

Events: 8342
Events per second: 118.988
jumped back (PMT): 18
#Frames read, frequency
# 0 frames :213575
# 1 frames :54895
# 2 frames :2697
# 3 frames :908
# 4 frames :399
# 5 frames :175
# 6 frames :2269
# 7 frames :1039
# 8 frames :1083
# 9 frames :502
# 10 frames :319
# 11 frames :343
# 12 frames :260
# 13 frames :158
# 14 frames :242
# 15 frames :187
# 16 frames :151
# 17 frames :124
# 18 frames :91
# 19 frames :62
# 20 frames :79
# 21 frames :52
# 22 frames :55
# 23 frames :55
# 24 frames :39
# 25 frames :32
# 26 frames :24
# 27 frames :25
# 28 frames :11
# 29 frames :17
# 30 frames :19
# 31 frames :17
# 32 frames :15
# 33 frames :9
# 34 frames :11
# 35 frames :6
# 36 frames :7
# 37 frames :4
# 38 frames :7
# 39 frames :5
# 40 frames :5
# 41 frames :2
# 42 frames :2
# 43 frames :4
# 44 frames :2
# 45 frames :3
# 48 frames :2
# 49 frames :1
# 50 frames :2
# 51 frames :1
# 52 frames :1
# 55 frames :1
# 58 frames :2
# 60 frames :1
# 69 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.8907
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0