Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "62549ac5490b06ded1b1a4f9619ec0b5",
"name": "M1_20170518_1426_dsRNA_H4_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:35:39"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8459 (max 8460) in '8459'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.0067
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.81004e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 15 mean_circ: 0.954186
Ran for 1050 frames Cell Events: 46 mean_circ: 0.941922
Ran for 2050 frames Cell Events: 95 mean_circ: 0.93142
Ran for 2550 frames Cell Events: 118 mean_circ: 0.935604
Ran for 3050 frames Cell Events: 144 mean_circ: 0.936635
Ran for 5050 frames Cell Events: 240 mean_circ: 0.940416
Ran for 5550 frames Cell Events: 278 mean_circ: 0.941954
Ran for 7550 frames Cell Events: 409 mean_circ: 0.943324
Ran for 8550 frames Cell Events: 479 mean_circ: 0.944099
Ran for 9550 frames Cell Events: 539 mean_circ: 0.943902
Ran for 10550 frames Cell Events: 588 mean_circ: 0.943936
Ran for 11050 frames Cell Events: 623 mean_circ: 0.943947
Ran for 12550 frames Cell Events: 702 mean_circ: 0.943476
Ran for 13550 frames Cell Events: 761 mean_circ: 0.943669
Ran for 14050 frames Cell Events: 785 mean_circ: 0.943379
Ran for 14550 frames Cell Events: 809 mean_circ: 0.943403
Ran for 16050 frames Cell Events: 898 mean_circ: 0.942905
Ran for 16550 frames Cell Events: 930 mean_circ: 0.942627
Ran for 17050 frames Cell Events: 966 mean_circ: 0.942699
Ran for 17550 frames Cell Events: 999 mean_circ: 0.942699
Ran for 18550 frames Cell Events: 1054 mean_circ: 0.942363
Ran for 20050 frames Cell Events: 1156 mean_circ: 0.941924
Ran for 23050 frames Cell Events: 1346 mean_circ: 0.941421
Ran for 23550 frames Cell Events: 1375 mean_circ: 0.941472
Ran for 24050 frames Cell Events: 1398 mean_circ: 0.94151
Ran for 24550 frames Cell Events: 1428 mean_circ: 0.941636
Ran for 25050 frames Cell Events: 1467 mean_circ: 0.94189
Ran for 26550 frames Cell Events: 1554 mean_circ: 0.94191
Ran for 28050 frames Cell Events: 1655 mean_circ: 0.941715
Ran for 29050 frames Cell Events: 1706 mean_circ: 0.941974
Ran for 30050 frames Cell Events: 1765 mean_circ: 0.94224
Ran for 30550 frames Cell Events: 1792 mean_circ: 0.942222
Ran for 31050 frames Cell Events: 1819 mean_circ: 0.941953
Ran for 31550 frames Cell Events: 1845 mean_circ: 0.941985
Ran for 32050 frames Cell Events: 1869 mean_circ: 0.941983
Ran for 33550 frames Cell Events: 1969 mean_circ: 0.941913
Ran for 34550 frames Cell Events: 2033 mean_circ: 0.941951
Ran for 35050 frames Cell Events: 2055 mean_circ: 0.941773
Ran for 35550 frames Cell Events: 2091 mean_circ: 0.941735
Ran for 36550 frames Cell Events: 2148 mean_circ: 0.941752
Ran for 37050 frames Cell Events: 2179 mean_circ: 0.941874
Ran for 37550 frames Cell Events: 2211 mean_circ: 0.941933
Ran for 38050 frames Cell Events: 2250 mean_circ: 0.941987
Ran for 39050 frames Cell Events: 2318 mean_circ: 0.942131
Ran for 39550 frames Cell Events: 2340 mean_circ: 0.942113
Ran for 41050 frames Cell Events: 2436 mean_circ: 0.94193
Ran for 41550 frames Cell Events: 2468 mean_circ: 0.941902
Ran for 42550 frames Cell Events: 2524 mean_circ: 0.941887
Ran for 44550 frames Cell Events: 2632 mean_circ: 0.942153
Ran for 45550 frames Cell Events: 2676 mean_circ: 0.942265
Ran for 46050 frames Cell Events: 2707 mean_circ: 0.942278
Ran for 47050 frames Cell Events: 2771 mean_circ: 0.941926
Ran for 48050 frames Cell Events: 2829 mean_circ: 0.94193
Ran for 49050 frames Cell Events: 2891 mean_circ: 0.942035
Ran for 50050 frames Cell Events: 2949 mean_circ: 0.94207
Ran for 52550 frames Cell Events: 3069 mean_circ: 0.942151
Ran for 53050 frames Cell Events: 3099 mean_circ: 0.942056
Ran for 53550 frames Cell Events: 3132 mean_circ: 0.942035
Ran for 54050 frames Cell Events: 3161 mean_circ: 0.94196
Ran for 55050 frames Cell Events: 3203 mean_circ: 0.942037
Ran for 56050 frames Cell Events: 3252 mean_circ: 0.942005
Ran for 56550 frames Cell Events: 3279 mean_circ: 0.941907
Ran for 58050 frames Cell Events: 3378 mean_circ: 0.941669
Ran for 58550 frames Cell Events: 3405 mean_circ: 0.941643
Ran for 59050 frames Cell Events: 3434 mean_circ: 0.941583
Ran for 60050 frames Cell Events: 3491 mean_circ: 0.94159
Ran for 61550 frames Cell Events: 3589 mean_circ: 0.941646
Ran for 62050 frames Cell Events: 3608 mean_circ: 0.941685
Ran for 62550 frames Cell Events: 3638 mean_circ: 0.941737
Ran for 63550 frames Cell Events: 3700 mean_circ: 0.941871
Ran for 64550 frames Cell Events: 3748 mean_circ: 0.941953
Ran for 66550 frames Cell Events: 3863 mean_circ: 0.94206
Ran for 68050 frames Cell Events: 3944 mean_circ: 0.94208
Ran for 68550 frames Cell Events: 3975 mean_circ: 0.941982
Ran for 70050 frames Cell Events: 4063 mean_circ: 0.941854
Ran for 74050 frames Cell Events: 4356 mean_circ: 0.941791
Ran for 74550 frames Cell Events: 4384 mean_circ: 0.941815
Ran for 75050 frames Cell Events: 4417 mean_circ: 0.941792
Ran for 75550 frames Cell Events: 4447 mean_circ: 0.941777
Ran for 76050 frames Cell Events: 4464 mean_circ: 0.941847
Ran for 77550 frames Cell Events: 4557 mean_circ: 0.941819
Ran for 79050 frames Cell Events: 4654 mean_circ: 0.941786
Ran for 79550 frames Cell Events: 4692 mean_circ: 0.941785
Ran for 80550 frames Cell Events: 4762 mean_circ: 0.941656
Ran for 81050 frames Cell Events: 4792 mean_circ: 0.941622
Ran for 82550 frames Cell Events: 4888 mean_circ: 0.941545
Ran for 83050 frames Cell Events: 4940 mean_circ: 0.941545
Ran for 84050 frames Cell Events: 5009 mean_circ: 0.941541
Ran for 84550 frames Cell Events: 5055 mean_circ: 0.941576
Ran for 85050 frames Cell Events: 5085 mean_circ: 0.941539
Ran for 85550 frames Cell Events: 5109 mean_circ: 0.941541
Ran for 86050 frames Cell Events: 5137 mean_circ: 0.941556
Ran for 86550 frames Cell Events: 5167 mean_circ: 0.941532
Ran for 87050 frames Cell Events: 5203 mean_circ: 0.94151
Ran for 88050 frames Cell Events: 5257 mean_circ: 0.941578
Ran for 89050 frames Cell Events: 5334 mean_circ: 0.941427
Ran for 89550 frames Cell Events: 5357 mean_circ: 0.941381
Ran for 91050 frames Cell Events: 5440 mean_circ: 0.941316
Ran for 91550 frames Cell Events: 5465 mean_circ: 0.94135
Ran for 92050 frames Cell Events: 5501 mean_circ: 0.941374
Ran for 93050 frames Cell Events: 5570 mean_circ: 0.941288
Ran for 93550 frames Cell Events: 5599 mean_circ: 0.941292
Ran for 94050 frames Cell Events: 5636 mean_circ: 0.941237
Ran for 95050 frames Cell Events: 5714 mean_circ: 0.941131
Ran for 95550 frames Cell Events: 5738 mean_circ: 0.941155
Ran for 96050 frames Cell Events: 5775 mean_circ: 0.941169
Ran for 97550 frames Cell Events: 5862 mean_circ: 0.941133
Ran for 98050 frames Cell Events: 5899 mean_circ: 0.941157
Ran for 98550 frames Cell Events: 5934 mean_circ: 0.94109
Ran for 99550 frames Cell Events: 6005 mean_circ: 0.941094
Ran for 101050 frames Cell Events: 6100 mean_circ: 0.941102
Ran for 103550 frames Cell Events: 6263 mean_circ: 0.941061
Ran for 104050 frames Cell Events: 6291 mean_circ: 0.941066
Ran for 104550 frames Cell Events: 6322 mean_circ: 0.941055
Ran for 105050 frames Cell Events: 6367 mean_circ: 0.941014
Ran for 106550 frames Cell Events: 6472 mean_circ: 0.940828
Ran for 107050 frames Cell Events: 6490 mean_circ: 0.940836
Ran for 107550 frames Cell Events: 6520 mean_circ: 0.940833
Ran for 109550 frames Cell Events: 6671 mean_circ: 0.940855
Ran for 110050 frames Cell Events: 6702 mean_circ: 0.940864
Ran for 110550 frames Cell Events: 6735 mean_circ: 0.940854
Ran for 111550 frames Cell Events: 6800 mean_circ: 0.940855
Ran for 112050 frames Cell Events: 6838 mean_circ: 0.940852
Ran for 112550 frames Cell Events: 6869 mean_circ: 0.940844
Ran for 114050 frames Cell Events: 6973 mean_circ: 0.940801
Ran for 115550 frames Cell Events: 7048 mean_circ: 0.940798
Ran for 116550 frames Cell Events: 7112 mean_circ: 0.940723
Ran for 117050 frames Cell Events: 7156 mean_circ: 0.940713
Ran for 117550 frames Cell Events: 7202 mean_circ: 0.940713
Ran for 119550 frames Cell Events: 7349 mean_circ: 0.940614
Ran for 120550 frames Cell Events: 7408 mean_circ: 0.940645
Ran for 121550 frames Cell Events: 7465 mean_circ: 0.940619
Ran for 122050 frames Cell Events: 7494 mean_circ: 0.940618
Ran for 122550 frames Cell Events: 7516 mean_circ: 0.940668
Ran for 124550 frames Cell Events: 7610 mean_circ: 0.940688
Ran for 125050 frames Cell Events: 7638 mean_circ: 0.940674
Ran for 127050 frames Cell Events: 7756 mean_circ: 0.940654
Ran for 127550 frames Cell Events: 7785 mean_circ: 0.940674
Ran for 131550 frames Cell Events: 8034 mean_circ: 0.940674
Ran for 132550 frames Cell Events: 8093 mean_circ: 0.940676
Ran for 133050 frames Cell Events: 8125 mean_circ: 0.940704
Ran for 133550 frames Cell Events: 8166 mean_circ: 0.940629
Ran for 135050 frames Cell Events: 8261 mean_circ: 0.940649
Ran for 136550 frames Cell Events: 8361 mean_circ: 0.940662
Ran for 138050 frames Cell Events: 8457 mean_circ: 0.94065



End this measurment..



Stopping time: 2.81073e+008
****************************************
Measurement finished

Mean Circularitiy: 0.940672
Mean Area: 605.164
Mean FL-1 intensity: 3103.73
Mean FL-2 intensity: 4879.56
Mean FL-3 intensity: 0

frames processed: 136647
Total Time: 69128.3ms
Number of Runs (new bg img) 0
Frames collected: 138081
Skipped frames: 26188
Rescued Frames: 1540456
Samples DAQ: 34520560
Samples written: 69040380
Samples per Channel: 34520190
FPS: 1997.46

Samples per second: 499364
Expected samples/s: 500000

Events: 8460
Events per second: 122.381
jumped back (PMT): 15
#Frames read, frequency
# 0 frames :209340
# 1 frames :54944
# 2 frames :2757
# 3 frames :935
# 4 frames :374
# 5 frames :179
# 6 frames :2577
# 7 frames :1117
# 8 frames :1199
# 9 frames :498
# 10 frames :344
# 11 frames :234
# 12 frames :200
# 13 frames :138
# 14 frames :315
# 15 frames :175
# 16 frames :142
# 17 frames :100
# 18 frames :61
# 19 frames :38
# 20 frames :66
# 21 frames :58
# 22 frames :47
# 23 frames :32
# 24 frames :34
# 25 frames :28
# 26 frames :16
# 27 frames :12
# 28 frames :18
# 29 frames :12
# 30 frames :14
# 31 frames :20
# 32 frames :8
# 33 frames :6
# 34 frames :4
# 35 frames :6
# 36 frames :3
# 37 frames :2
# 38 frames :2
# 39 frames :2
# 40 frames :1
# 41 frames :6
# 43 frames :3
# 44 frames :3
# 45 frames :3
# 46 frames :3
# 47 frames :2
# 48 frames :1
# 49 frames :1
# 51 frames :1
# 57 frames :1
# 58 frames :1
# 60 frames :1
# 63 frames :1
# 72 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.0067
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0