Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "1e9005445e572ce7cbc147737e208322",
"name": "M1_20170518_1511_dsRNA_H6_col.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:37:41"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8327 (max 8328) in '8327'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.2049
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.83711e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 170 mean_circ: 0.945445
Ran for 2550 frames Cell Events: 189 mean_circ: 0.945765
Ran for 3050 frames Cell Events: 208 mean_circ: 0.948602
Ran for 4050 frames Cell Events: 289 mean_circ: 0.948864
Ran for 4550 frames Cell Events: 315 mean_circ: 0.946128
Ran for 5050 frames Cell Events: 340 mean_circ: 0.946017
Ran for 6050 frames Cell Events: 381 mean_circ: 0.946652
Ran for 7050 frames Cell Events: 446 mean_circ: 0.946989
Ran for 8550 frames Cell Events: 550 mean_circ: 0.94727
Ran for 10050 frames Cell Events: 660 mean_circ: 0.946523
Ran for 10550 frames Cell Events: 680 mean_circ: 0.947011
Ran for 11050 frames Cell Events: 715 mean_circ: 0.947011
Ran for 11550 frames Cell Events: 740 mean_circ: 0.947304
Ran for 12550 frames Cell Events: 804 mean_circ: 0.947244
Ran for 13550 frames Cell Events: 863 mean_circ: 0.947067
Ran for 14050 frames Cell Events: 892 mean_circ: 0.947257
Ran for 15550 frames Cell Events: 993 mean_circ: 0.947652
Ran for 17550 frames Cell Events: 1151 mean_circ: 0.947073
Ran for 18550 frames Cell Events: 1205 mean_circ: 0.947417
Ran for 19050 frames Cell Events: 1245 mean_circ: 0.947706
Ran for 19550 frames Cell Events: 1272 mean_circ: 0.947879
Ran for 21550 frames Cell Events: 1392 mean_circ: 0.947817
Ran for 22550 frames Cell Events: 1460 mean_circ: 0.947706
Ran for 23050 frames Cell Events: 1491 mean_circ: 0.947718
Ran for 24550 frames Cell Events: 1590 mean_circ: 0.947921
Ran for 25050 frames Cell Events: 1604 mean_circ: 0.94787
Ran for 25550 frames Cell Events: 1640 mean_circ: 0.94796
Ran for 26050 frames Cell Events: 1657 mean_circ: 0.947925
Ran for 28050 frames Cell Events: 1764 mean_circ: 0.948095
Ran for 28550 frames Cell Events: 1801 mean_circ: 0.947824
Ran for 31050 frames Cell Events: 1992 mean_circ: 0.947755
Ran for 31550 frames Cell Events: 2017 mean_circ: 0.947904
Ran for 32050 frames Cell Events: 2033 mean_circ: 0.947991
Ran for 34050 frames Cell Events: 2154 mean_circ: 0.947875
Ran for 34550 frames Cell Events: 2186 mean_circ: 0.947962
Ran for 35050 frames Cell Events: 2214 mean_circ: 0.947885
Ran for 35550 frames Cell Events: 2239 mean_circ: 0.94792
Ran for 36050 frames Cell Events: 2269 mean_circ: 0.947997
Ran for 36550 frames Cell Events: 2290 mean_circ: 0.947969
Ran for 37550 frames Cell Events: 2344 mean_circ: 0.94812
Ran for 38050 frames Cell Events: 2389 mean_circ: 0.94812
Ran for 39050 frames Cell Events: 2443 mean_circ: 0.948413
Ran for 39550 frames Cell Events: 2471 mean_circ: 0.948415
Ran for 40550 frames Cell Events: 2546 mean_circ: 0.948337
Ran for 41050 frames Cell Events: 2573 mean_circ: 0.948309
Ran for 41550 frames Cell Events: 2609 mean_circ: 0.948259
Ran for 42050 frames Cell Events: 2647 mean_circ: 0.948274
Ran for 42550 frames Cell Events: 2682 mean_circ: 0.948325
Ran for 43050 frames Cell Events: 2719 mean_circ: 0.948377
Ran for 44550 frames Cell Events: 2832 mean_circ: 0.948529
Ran for 46050 frames Cell Events: 2956 mean_circ: 0.948469
Ran for 47550 frames Cell Events: 3083 mean_circ: 0.948591
Ran for 49050 frames Cell Events: 3176 mean_circ: 0.948576
Ran for 50050 frames Cell Events: 3245 mean_circ: 0.948595
Ran for 51050 frames Cell Events: 3309 mean_circ: 0.948602
Ran for 53550 frames Cell Events: 3501 mean_circ: 0.948824
Ran for 56550 frames Cell Events: 3760 mean_circ: 0.948696
Ran for 57050 frames Cell Events: 3787 mean_circ: 0.948675
Ran for 57550 frames Cell Events: 3813 mean_circ: 0.948586
Ran for 58550 frames Cell Events: 3893 mean_circ: 0.948464
Ran for 59550 frames Cell Events: 3975 mean_circ: 0.948355
Ran for 60050 frames Cell Events: 4009 mean_circ: 0.948426
Ran for 60550 frames Cell Events: 4052 mean_circ: 0.948448
Ran for 61550 frames Cell Events: 4116 mean_circ: 0.948466
Ran for 64050 frames Cell Events: 4324 mean_circ: 0.948186
Ran for 64550 frames Cell Events: 4351 mean_circ: 0.948178
Ran for 65050 frames Cell Events: 4371 mean_circ: 0.948137
Ran for 67550 frames Cell Events: 4515 mean_circ: 0.948161
Ran for 68050 frames Cell Events: 4548 mean_circ: 0.948191
Ran for 68550 frames Cell Events: 4582 mean_circ: 0.948219
Ran for 69050 frames Cell Events: 4605 mean_circ: 0.948254
Ran for 71050 frames Cell Events: 4744 mean_circ: 0.948316
Ran for 72550 frames Cell Events: 4825 mean_circ: 0.948241
Ran for 73050 frames Cell Events: 4852 mean_circ: 0.948194
Ran for 73550 frames Cell Events: 4888 mean_circ: 0.948114
Ran for 75550 frames Cell Events: 5034 mean_circ: 0.948084
Ran for 76050 frames Cell Events: 5059 mean_circ: 0.948032
Ran for 77550 frames Cell Events: 5173 mean_circ: 0.948117
Ran for 78050 frames Cell Events: 5206 mean_circ: 0.948038
Ran for 79550 frames Cell Events: 5311 mean_circ: 0.947874
Ran for 80050 frames Cell Events: 5354 mean_circ: 0.947801
Ran for 81050 frames Cell Events: 5414 mean_circ: 0.947708
Ran for 81550 frames Cell Events: 5443 mean_circ: 0.947729
Ran for 82050 frames Cell Events: 5465 mean_circ: 0.94774
Ran for 84050 frames Cell Events: 5591 mean_circ: 0.947856
Ran for 84550 frames Cell Events: 5610 mean_circ: 0.947853
Ran for 85050 frames Cell Events: 5643 mean_circ: 0.947853
Ran for 85550 frames Cell Events: 5688 mean_circ: 0.947825
Ran for 88550 frames Cell Events: 5880 mean_circ: 0.947646
Ran for 90050 frames Cell Events: 6013 mean_circ: 0.947708
Ran for 91050 frames Cell Events: 6069 mean_circ: 0.947765
Ran for 93050 frames Cell Events: 6220 mean_circ: 0.947643
Ran for 93550 frames Cell Events: 6266 mean_circ: 0.947618
Ran for 94550 frames Cell Events: 6338 mean_circ: 0.947643
Ran for 95050 frames Cell Events: 6362 mean_circ: 0.947546
Ran for 100050 frames Cell Events: 6689 mean_circ: 0.94756
Ran for 101550 frames Cell Events: 6797 mean_circ: 0.94759
Ran for 102550 frames Cell Events: 6854 mean_circ: 0.947558
Ran for 104050 frames Cell Events: 6950 mean_circ: 0.947559
Ran for 105050 frames Cell Events: 6998 mean_circ: 0.947578
Ran for 105550 frames Cell Events: 7022 mean_circ: 0.947577
Ran for 106050 frames Cell Events: 7041 mean_circ: 0.9476
Ran for 107050 frames Cell Events: 7096 mean_circ: 0.947653
Ran for 108550 frames Cell Events: 7207 mean_circ: 0.947637
Ran for 110550 frames Cell Events: 7317 mean_circ: 0.947616
Ran for 116050 frames Cell Events: 7693 mean_circ: 0.947631
Ran for 116550 frames Cell Events: 7723 mean_circ: 0.947657
Ran for 118550 frames Cell Events: 7857 mean_circ: 0.947551
Ran for 119050 frames Cell Events: 7881 mean_circ: 0.947581
Ran for 120550 frames Cell Events: 7970 mean_circ: 0.947532
Ran for 121050 frames Cell Events: 7993 mean_circ: 0.94753
Ran for 121550 frames Cell Events: 8016 mean_circ: 0.947509
Ran for 122550 frames Cell Events: 8075 mean_circ: 0.947417
Ran for 124050 frames Cell Events: 8180 mean_circ: 0.94747
Ran for 125050 frames Cell Events: 8222 mean_circ: 0.947507
Ran for 125550 frames Cell Events: 8263 mean_circ: 0.947507



End this measurment..



Stopping time: 2.83774e+008
****************************************
Measurement finished

Mean Circularitiy: 0.94744
Mean Area: 634.406
Mean FL-1 intensity: 3543.48
Mean FL-2 intensity: 4916.97
Mean FL-3 intensity: 0

frames processed: 123904
Total Time: 63282.1ms
Number of Runs (new bg img) 0
Frames collected: 126410
Skipped frames: 37971
Rescued Frames: 2198706
Samples DAQ: 31604493
Samples written: 63204968
Samples per Channel: 31602484
FPS: 1997.56

Samples per second: 499391
Expected samples/s: 500000

Events: 8328
Events per second: 131.601
jumped back (PMT): 2476
#Frames read, frequency
# 0 frames :203807
# 1 frames :39039
# 2 frames :2113
# 3 frames :792
# 4 frames :450
# 5 frames :218
# 6 frames :967
# 7 frames :1144
# 8 frames :655
# 9 frames :481
# 10 frames :615
# 11 frames :301
# 12 frames :242
# 13 frames :163
# 14 frames :169
# 15 frames :242
# 16 frames :154
# 17 frames :160
# 18 frames :126
# 19 frames :72
# 20 frames :74
# 21 frames :101
# 22 frames :84
# 23 frames :55
# 24 frames :47
# 25 frames :43
# 26 frames :37
# 27 frames :42
# 28 frames :35
# 29 frames :33
# 30 frames :29
# 31 frames :32
# 32 frames :27
# 33 frames :21
# 34 frames :16
# 35 frames :15
# 36 frames :13
# 37 frames :9
# 38 frames :12
# 39 frames :15
# 40 frames :18
# 41 frames :8
# 42 frames :10
# 43 frames :15
# 44 frames :6
# 45 frames :3
# 46 frames :5
# 47 frames :5
# 48 frames :5
# 49 frames :3
# 50 frames :1
# 51 frames :2
# 52 frames :3
# 53 frames :2
# 54 frames :4
# 55 frames :5
# 56 frames :5
# 57 frames :2
# 58 frames :3
# 59 frames :3
# 60 frames :1
# 61 frames :2
# 62 frames :2
# 63 frames :1
# 69 frames :2
# 70 frames :1
# 71 frames :1
# 74 frames :1
# 81 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.2049
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0