Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "829f7870ad097b5180c5a8da5cedb169",
"name": "M1_20170518_1513_dsRNA_H6_fast.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:37:58"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8120 (max 8121) in '8120'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.2222
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.83871e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 31 mean_circ: 0.935987
Ran for 1550 frames Cell Events: 106 mean_circ: 0.943632
Ran for 2050 frames Cell Events: 135 mean_circ: 0.941642
Ran for 2550 frames Cell Events: 161 mean_circ: 0.940581
Ran for 3550 frames Cell Events: 234 mean_circ: 0.937832
Ran for 4550 frames Cell Events: 307 mean_circ: 0.938064
Ran for 6050 frames Cell Events: 394 mean_circ: 0.937191
Ran for 6550 frames Cell Events: 414 mean_circ: 0.937362
Ran for 7550 frames Cell Events: 490 mean_circ: 0.936777
Ran for 9550 frames Cell Events: 631 mean_circ: 0.937538
Ran for 10550 frames Cell Events: 669 mean_circ: 0.936354
Ran for 11550 frames Cell Events: 718 mean_circ: 0.936581
Ran for 13550 frames Cell Events: 877 mean_circ: 0.937974
Ran for 14550 frames Cell Events: 938 mean_circ: 0.93827
Ran for 16050 frames Cell Events: 1023 mean_circ: 0.938569
Ran for 17050 frames Cell Events: 1094 mean_circ: 0.938487
Ran for 19550 frames Cell Events: 1306 mean_circ: 0.939034
Ran for 20550 frames Cell Events: 1373 mean_circ: 0.938484
Ran for 21050 frames Cell Events: 1396 mean_circ: 0.938588
Ran for 22550 frames Cell Events: 1504 mean_circ: 0.938355
Ran for 23050 frames Cell Events: 1532 mean_circ: 0.938544
Ran for 23550 frames Cell Events: 1562 mean_circ: 0.938804
Ran for 25050 frames Cell Events: 1676 mean_circ: 0.938419
Ran for 26550 frames Cell Events: 1809 mean_circ: 0.938733
Ran for 30050 frames Cell Events: 2086 mean_circ: 0.937959
Ran for 30550 frames Cell Events: 2126 mean_circ: 0.937746
Ran for 33050 frames Cell Events: 2315 mean_circ: 0.93799
Ran for 33550 frames Cell Events: 2344 mean_circ: 0.938098
Ran for 34050 frames Cell Events: 2377 mean_circ: 0.937996
Ran for 35050 frames Cell Events: 2445 mean_circ: 0.938071
Ran for 35550 frames Cell Events: 2481 mean_circ: 0.938072
Ran for 37050 frames Cell Events: 2577 mean_circ: 0.938102
Ran for 37550 frames Cell Events: 2612 mean_circ: 0.938347
Ran for 38050 frames Cell Events: 2638 mean_circ: 0.938512
Ran for 38550 frames Cell Events: 2654 mean_circ: 0.938419
Ran for 39050 frames Cell Events: 2687 mean_circ: 0.938504
Ran for 40550 frames Cell Events: 2802 mean_circ: 0.938567
Ran for 41050 frames Cell Events: 2842 mean_circ: 0.938553
Ran for 42050 frames Cell Events: 2904 mean_circ: 0.938739
Ran for 42550 frames Cell Events: 2944 mean_circ: 0.938679
Ran for 45050 frames Cell Events: 3186 mean_circ: 0.937947
Ran for 45550 frames Cell Events: 3219 mean_circ: 0.937797
Ran for 46050 frames Cell Events: 3266 mean_circ: 0.937797
Ran for 46550 frames Cell Events: 3305 mean_circ: 0.937585
Ran for 50050 frames Cell Events: 3545 mean_circ: 0.937415
Ran for 50550 frames Cell Events: 3580 mean_circ: 0.937377
Ran for 51050 frames Cell Events: 3619 mean_circ: 0.937296
Ran for 51550 frames Cell Events: 3651 mean_circ: 0.93715
Ran for 53050 frames Cell Events: 3749 mean_circ: 0.936918
Ran for 53550 frames Cell Events: 3792 mean_circ: 0.936828
Ran for 54050 frames Cell Events: 3828 mean_circ: 0.936828
Ran for 55050 frames Cell Events: 3911 mean_circ: 0.936837
Ran for 55550 frames Cell Events: 3948 mean_circ: 0.93693
Ran for 57550 frames Cell Events: 4087 mean_circ: 0.936743
Ran for 58050 frames Cell Events: 4111 mean_circ: 0.936663
Ran for 60050 frames Cell Events: 4260 mean_circ: 0.936928
Ran for 62550 frames Cell Events: 4405 mean_circ: 0.93687
Ran for 65050 frames Cell Events: 4566 mean_circ: 0.936795
Ran for 66050 frames Cell Events: 4634 mean_circ: 0.936727
Ran for 68050 frames Cell Events: 4799 mean_circ: 0.936654
Ran for 70050 frames Cell Events: 4965 mean_circ: 0.936786
Ran for 70550 frames Cell Events: 4987 mean_circ: 0.93668
Ran for 71050 frames Cell Events: 5022 mean_circ: 0.936631
Ran for 71550 frames Cell Events: 5052 mean_circ: 0.936651
Ran for 72050 frames Cell Events: 5080 mean_circ: 0.936619
Ran for 72550 frames Cell Events: 5111 mean_circ: 0.936674
Ran for 73050 frames Cell Events: 5137 mean_circ: 0.936669
Ran for 73550 frames Cell Events: 5179 mean_circ: 0.936685
Ran for 74050 frames Cell Events: 5218 mean_circ: 0.936685
Ran for 74550 frames Cell Events: 5238 mean_circ: 0.936723
Ran for 75050 frames Cell Events: 5270 mean_circ: 0.936704
Ran for 75550 frames Cell Events: 5317 mean_circ: 0.936849
Ran for 76050 frames Cell Events: 5346 mean_circ: 0.936857
Ran for 78550 frames Cell Events: 5548 mean_circ: 0.936951
Ran for 80550 frames Cell Events: 5714 mean_circ: 0.937024
Ran for 81050 frames Cell Events: 5738 mean_circ: 0.937027
Ran for 82050 frames Cell Events: 5828 mean_circ: 0.936931
Ran for 84550 frames Cell Events: 6032 mean_circ: 0.937068
Ran for 85050 frames Cell Events: 6080 mean_circ: 0.937074
Ran for 86050 frames Cell Events: 6166 mean_circ: 0.937125
Ran for 86550 frames Cell Events: 6205 mean_circ: 0.9371
Ran for 88050 frames Cell Events: 6330 mean_circ: 0.937084
Ran for 89550 frames Cell Events: 6466 mean_circ: 0.93701
Ran for 90050 frames Cell Events: 6501 mean_circ: 0.937041
Ran for 92550 frames Cell Events: 6667 mean_circ: 0.937022
Ran for 93050 frames Cell Events: 6707 mean_circ: 0.937022
Ran for 93550 frames Cell Events: 6743 mean_circ: 0.937059
Ran for 97550 frames Cell Events: 7037 mean_circ: 0.936936
Ran for 99050 frames Cell Events: 7179 mean_circ: 0.936921
Ran for 100050 frames Cell Events: 7270 mean_circ: 0.936893
Ran for 100550 frames Cell Events: 7313 mean_circ: 0.936863
Ran for 101050 frames Cell Events: 7346 mean_circ: 0.936925
Ran for 101550 frames Cell Events: 7370 mean_circ: 0.93696
Ran for 102550 frames Cell Events: 7398 mean_circ: 0.937003
Ran for 103050 frames Cell Events: 7416 mean_circ: 0.936929
Ran for 103550 frames Cell Events: 7463 mean_circ: 0.936916
Ran for 104550 frames Cell Events: 7539 mean_circ: 0.936954
Ran for 106050 frames Cell Events: 7625 mean_circ: 0.936992
Ran for 108550 frames Cell Events: 7779 mean_circ: 0.937067
Ran for 109550 frames Cell Events: 7854 mean_circ: 0.937074
Ran for 112050 frames Cell Events: 8065 mean_circ: 0.937039



End this measurment..



Stopping time: 2.83928e+008
****************************************
Measurement finished

Mean Circularitiy: 0.936972
Mean Area: 639.124
Mean FL-1 intensity: 3368.46
Mean FL-2 intensity: 5155.24
Mean FL-3 intensity: 0

frames processed: 109627
Total Time: 56466.1ms
Number of Runs (new bg img) 0
Frames collected: 112759
Skipped frames: 41103
Rescued Frames: 2264569
Samples DAQ: 28189791
Samples written: 56379242
Samples per Channel: 28189621
FPS: 1996.93

Samples per second: 499230
Expected samples/s: 500000

Events: 8121
Events per second: 143.821
jumped back (PMT): 23
#Frames read, frequency
# 0 frames :179824
# 1 frames :34555
# 2 frames :1997
# 3 frames :638
# 4 frames :294
# 5 frames :125
# 6 frames :1817
# 7 frames :773
# 8 frames :886
# 9 frames :427
# 10 frames :315
# 11 frames :195
# 12 frames :170
# 13 frames :134
# 14 frames :220
# 15 frames :172
# 16 frames :144
# 17 frames :89
# 18 frames :68
# 19 frames :74
# 20 frames :65
# 21 frames :66
# 22 frames :49
# 23 frames :64
# 24 frames :44
# 25 frames :30
# 26 frames :25
# 27 frames :27
# 28 frames :31
# 29 frames :32
# 30 frames :32
# 31 frames :14
# 32 frames :19
# 33 frames :20
# 34 frames :11
# 35 frames :28
# 36 frames :13
# 37 frames :14
# 38 frames :11
# 39 frames :4
# 40 frames :15
# 41 frames :10
# 42 frames :9
# 43 frames :5
# 44 frames :9
# 45 frames :5
# 46 frames :4
# 47 frames :2
# 48 frames :3
# 49 frames :4
# 50 frames :6
# 51 frames :5
# 52 frames :5
# 53 frames :1
# 55 frames :5
# 56 frames :2
# 57 frames :2
# 59 frames :1
# 61 frames :1
# 62 frames :1
# 64 frames :1
# 65 frames :2
# 66 frames :1
# 67 frames :1
# 72 frames :1
# 74 frames :1
# 75 frames :1
# 77 frames :1
# 80 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.2222
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0