Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fc65c18aca1ce8cc5fc5dd7b73bad744",
"name": "M1_20170518_1525_dsRNA_H7_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:38:40"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.2565
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.84553e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 89 mean_circ: 0.94856
Ran for 2050 frames Cell Events: 152 mean_circ: 0.939956
Ran for 3050 frames Cell Events: 215 mean_circ: 0.941
Ran for 4050 frames Cell Events: 263 mean_circ: 0.941945
Ran for 6050 frames Cell Events: 365 mean_circ: 0.942964
Ran for 7050 frames Cell Events: 430 mean_circ: 0.942808
Ran for 7550 frames Cell Events: 463 mean_circ: 0.94333
Ran for 9050 frames Cell Events: 578 mean_circ: 0.944301
Ran for 9550 frames Cell Events: 611 mean_circ: 0.943713
Ran for 11050 frames Cell Events: 726 mean_circ: 0.944711
Ran for 11550 frames Cell Events: 756 mean_circ: 0.944447
Ran for 12050 frames Cell Events: 788 mean_circ: 0.944333
Ran for 12550 frames Cell Events: 827 mean_circ: 0.944333
Ran for 13050 frames Cell Events: 865 mean_circ: 0.94352
Ran for 13550 frames Cell Events: 889 mean_circ: 0.943027
Ran for 14050 frames Cell Events: 924 mean_circ: 0.942617
Ran for 15050 frames Cell Events: 968 mean_circ: 0.942125
Ran for 16050 frames Cell Events: 1034 mean_circ: 0.942304
Ran for 16550 frames Cell Events: 1063 mean_circ: 0.942352
Ran for 17050 frames Cell Events: 1097 mean_circ: 0.942644
Ran for 18550 frames Cell Events: 1220 mean_circ: 0.942321
Ran for 19550 frames Cell Events: 1291 mean_circ: 0.942221
Ran for 20050 frames Cell Events: 1334 mean_circ: 0.942033
Ran for 22050 frames Cell Events: 1461 mean_circ: 0.942049
Ran for 23050 frames Cell Events: 1537 mean_circ: 0.941934
Ran for 23550 frames Cell Events: 1585 mean_circ: 0.942028
Ran for 25050 frames Cell Events: 1669 mean_circ: 0.941983
Ran for 25550 frames Cell Events: 1692 mean_circ: 0.941857
Ran for 26050 frames Cell Events: 1724 mean_circ: 0.941892
Ran for 27050 frames Cell Events: 1797 mean_circ: 0.941824
Ran for 27550 frames Cell Events: 1835 mean_circ: 0.941813
Ran for 29050 frames Cell Events: 1948 mean_circ: 0.942417
Ran for 30050 frames Cell Events: 2002 mean_circ: 0.942474
Ran for 30550 frames Cell Events: 2040 mean_circ: 0.94249
Ran for 31550 frames Cell Events: 2109 mean_circ: 0.942541
Ran for 33050 frames Cell Events: 2212 mean_circ: 0.942447
Ran for 33550 frames Cell Events: 2242 mean_circ: 0.942498
Ran for 34550 frames Cell Events: 2309 mean_circ: 0.942268
Ran for 35550 frames Cell Events: 2370 mean_circ: 0.942241
Ran for 37050 frames Cell Events: 2480 mean_circ: 0.941831
Ran for 38050 frames Cell Events: 2539 mean_circ: 0.941882
Ran for 41050 frames Cell Events: 2727 mean_circ: 0.941827
Ran for 41550 frames Cell Events: 2748 mean_circ: 0.941825
Ran for 42050 frames Cell Events: 2778 mean_circ: 0.941849
Ran for 42550 frames Cell Events: 2804 mean_circ: 0.941849
Ran for 43050 frames Cell Events: 2837 mean_circ: 0.941849
Ran for 44050 frames Cell Events: 2898 mean_circ: 0.94184
Ran for 44550 frames Cell Events: 2922 mean_circ: 0.941859
Ran for 46550 frames Cell Events: 3047 mean_circ: 0.942309
Ran for 47050 frames Cell Events: 3073 mean_circ: 0.942258
Ran for 47550 frames Cell Events: 3108 mean_circ: 0.942322
Ran for 48050 frames Cell Events: 3138 mean_circ: 0.942351
Ran for 49050 frames Cell Events: 3196 mean_circ: 0.942287
Ran for 50050 frames Cell Events: 3253 mean_circ: 0.942306
Ran for 50550 frames Cell Events: 3296 mean_circ: 0.942328
Ran for 51050 frames Cell Events: 3337 mean_circ: 0.942346
Ran for 51550 frames Cell Events: 3365 mean_circ: 0.942308
Ran for 52050 frames Cell Events: 3408 mean_circ: 0.942358
Ran for 53050 frames Cell Events: 3485 mean_circ: 0.942273
Ran for 53550 frames Cell Events: 3516 mean_circ: 0.942301
Ran for 54050 frames Cell Events: 3545 mean_circ: 0.942191
Ran for 55050 frames Cell Events: 3626 mean_circ: 0.942061
Ran for 56550 frames Cell Events: 3746 mean_circ: 0.942097
Ran for 61050 frames Cell Events: 4078 mean_circ: 0.942051
Ran for 62050 frames Cell Events: 4142 mean_circ: 0.942218
Ran for 63050 frames Cell Events: 4194 mean_circ: 0.942267
Ran for 63550 frames Cell Events: 4239 mean_circ: 0.942267
Ran for 64550 frames Cell Events: 4307 mean_circ: 0.942215
Ran for 65050 frames Cell Events: 4340 mean_circ: 0.942171
Ran for 66550 frames Cell Events: 4424 mean_circ: 0.942142
Ran for 67050 frames Cell Events: 4470 mean_circ: 0.942072
Ran for 67550 frames Cell Events: 4495 mean_circ: 0.942053
Ran for 68550 frames Cell Events: 4558 mean_circ: 0.94201
Ran for 69050 frames Cell Events: 4592 mean_circ: 0.941981
Ran for 70050 frames Cell Events: 4658 mean_circ: 0.94191
Ran for 71050 frames Cell Events: 4727 mean_circ: 0.942017
Ran for 71550 frames Cell Events: 4763 mean_circ: 0.942075
Ran for 72050 frames Cell Events: 4811 mean_circ: 0.942052
Ran for 72550 frames Cell Events: 4842 mean_circ: 0.942071
Ran for 73550 frames Cell Events: 4939 mean_circ: 0.941993
Ran for 75050 frames Cell Events: 5055 mean_circ: 0.942061
Ran for 76050 frames Cell Events: 5110 mean_circ: 0.942032
Ran for 76550 frames Cell Events: 5151 mean_circ: 0.942058
Ran for 77050 frames Cell Events: 5182 mean_circ: 0.942123
Ran for 80050 frames Cell Events: 5377 mean_circ: 0.942091
Ran for 83050 frames Cell Events: 5576 mean_circ: 0.941843
Ran for 84050 frames Cell Events: 5654 mean_circ: 0.941855
Ran for 86050 frames Cell Events: 5781 mean_circ: 0.941984
Ran for 87550 frames Cell Events: 5886 mean_circ: 0.942054
Ran for 88050 frames Cell Events: 5921 mean_circ: 0.942061
Ran for 88550 frames Cell Events: 5946 mean_circ: 0.941995
Ran for 89050 frames Cell Events: 5971 mean_circ: 0.941991
Ran for 91050 frames Cell Events: 6107 mean_circ: 0.94199
Ran for 92550 frames Cell Events: 6211 mean_circ: 0.941883
Ran for 94050 frames Cell Events: 6346 mean_circ: 0.941833
Ran for 94550 frames Cell Events: 6373 mean_circ: 0.941923
Ran for 95550 frames Cell Events: 6447 mean_circ: 0.941812
Ran for 96050 frames Cell Events: 6480 mean_circ: 0.941834
Ran for 96550 frames Cell Events: 6509 mean_circ: 0.941815
Ran for 97050 frames Cell Events: 6540 mean_circ: 0.94178
Ran for 97550 frames Cell Events: 6570 mean_circ: 0.941688
Ran for 99050 frames Cell Events: 6685 mean_circ: 0.9417
Ran for 100550 frames Cell Events: 6789 mean_circ: 0.941697
Ran for 101550 frames Cell Events: 6864 mean_circ: 0.941635
Ran for 102550 frames Cell Events: 6922 mean_circ: 0.941611
Ran for 103050 frames Cell Events: 6958 mean_circ: 0.941596
Ran for 104550 frames Cell Events: 7055 mean_circ: 0.941625
Ran for 105050 frames Cell Events: 7093 mean_circ: 0.941632
Ran for 105550 frames Cell Events: 7132 mean_circ: 0.941626
Ran for 106050 frames Cell Events: 7163 mean_circ: 0.941655
Ran for 106550 frames Cell Events: 7195 mean_circ: 0.941615
Ran for 107550 frames Cell Events: 7267 mean_circ: 0.941587
Ran for 108050 frames Cell Events: 7289 mean_circ: 0.941528
Ran for 108550 frames Cell Events: 7317 mean_circ: 0.941523
Ran for 110050 frames Cell Events: 7428 mean_circ: 0.941465
Ran for 110550 frames Cell Events: 7457 mean_circ: 0.941475
Ran for 111550 frames Cell Events: 7534 mean_circ: 0.94152
Ran for 112550 frames Cell Events: 7603 mean_circ: 0.941479
Ran for 115550 frames Cell Events: 7805 mean_circ: 0.941423
Ran for 116050 frames Cell Events: 7848 mean_circ: 0.941423
Ran for 118550 frames Cell Events: 8034 mean_circ: 0.941321
Ran for 119050 frames Cell Events: 8063 mean_circ: 0.941277
Ran for 119550 frames Cell Events: 8094 mean_circ: 0.941268
Ran for 120050 frames Cell Events: 8127 mean_circ: 0.94124
Ran for 120550 frames Cell Events: 8161 mean_circ: 0.941259
Ran for 121050 frames Cell Events: 8193 mean_circ: 0.941231
Ran for 121550 frames Cell Events: 8224 mean_circ: 0.941226
Ran for 122050 frames Cell Events: 8247 mean_circ: 0.941226
Ran for 122550 frames Cell Events: 8280 mean_circ: 0.941274
Ran for 123550 frames Cell Events: 8337 mean_circ: 0.94131
Ran for 124550 frames Cell Events: 8406 mean_circ: 0.941223
Ran for 125550 frames Cell Events: 8462 mean_circ: 0.94126
Ran for 126550 frames Cell Events: 8529 mean_circ: 0.941262
Ran for 127050 frames Cell Events: 8563 mean_circ: 0.941286
Ran for 128050 frames Cell Events: 8630 mean_circ: 0.941323
Ran for 129050 frames Cell Events: 8701 mean_circ: 0.941309
Ran for 130050 frames Cell Events: 8767 mean_circ: 0.941248
Ran for 132050 frames Cell Events: 8907 mean_circ: 0.941168
Ran for 132550 frames Cell Events: 8956 mean_circ: 0.941171
Ran for 137050 frames Cell Events: 9291 mean_circ: 0.941286
Ran for 137550 frames Cell Events: 9312 mean_circ: 0.941293
Ran for 138050 frames Cell Events: 9346 mean_circ: 0.941261
Ran for 138550 frames Cell Events: 9381 mean_circ: 0.941279
Ran for 140050 frames Cell Events: 9490 mean_circ: 0.941263
Ran for 140550 frames Cell Events: 9533 mean_circ: 0.941214
Ran for 142050 frames Cell Events: 9644 mean_circ: 0.941191
Ran for 143550 frames Cell Events: 9759 mean_circ: 0.941132
Ran for 146050 frames Cell Events: 9949 mean_circ: 0.941129
Ran for 146550 frames Cell Events: 9986 mean_circ: 0.941118



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 2.84627e+008
****************************************
Measurement finished

Mean Circularitiy: 0.941138
Mean Area: 624.943
Mean FL-1 intensity: 3081.94
Mean FL-2 intensity: 4862.85
Mean FL-3 intensity: 0

frames processed: 145247
Total Time: 73437.3ms
Number of Runs (new bg img) 0
Frames collected: 146688
Skipped frames: 43726
Rescued Frames: 2431808
Samples DAQ: 36677043
Samples written: 73343886
Samples per Channel: 36671943
FPS: 1997.46

Samples per second: 499364
Expected samples/s: 500000

Events: 10002
Events per second: 136.198
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :229078
# 1 frames :51223
# 2 frames :2955
# 3 frames :960
# 4 frames :406
# 5 frames :201
# 6 frames :3004
# 7 frames :882
# 8 frames :1375
# 9 frames :580
# 10 frames :375
# 11 frames :214
# 12 frames :250
# 13 frames :137
# 14 frames :341
# 15 frames :214
# 16 frames :155
# 17 frames :138
# 18 frames :95
# 19 frames :56
# 20 frames :111
# 21 frames :70
# 22 frames :75
# 23 frames :47
# 24 frames :43
# 25 frames :36
# 26 frames :29
# 27 frames :17
# 28 frames :19
# 29 frames :16
# 30 frames :15
# 31 frames :23
# 32 frames :12
# 33 frames :8
# 34 frames :3
# 35 frames :8
# 36 frames :13
# 37 frames :7
# 38 frames :7
# 39 frames :5
# 40 frames :4
# 41 frames :5
# 42 frames :4
# 43 frames :4
# 44 frames :5
# 45 frames :4
# 46 frames :4
# 47 frames :1
# 48 frames :3
# 49 frames :5
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :2
# 56 frames :1
# 57 frames :1
# 58 frames :1
# 61 frames :1
# 65 frames :1
# 68 frames :2
# 69 frames :2
# 73 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.2565
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0