Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_17.58.14 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b68d55f9213f243f8fe945715dd365a5",
"name": "M1_20170518_1659_dsRNA_I2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:58:28"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.3913
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.90185e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.931907
Ran for 1050 frames Cell Events: 30 mean_circ: 0.961353
Ran for 2050 frames Cell Events: 58 mean_circ: 0.963137
Ran for 3050 frames Cell Events: 96 mean_circ: 0.96239
Ran for 3550 frames Cell Events: 117 mean_circ: 0.961731
Ran for 4050 frames Cell Events: 142 mean_circ: 0.961438
Ran for 4550 frames Cell Events: 159 mean_circ: 0.960182
Ran for 5050 frames Cell Events: 194 mean_circ: 0.958866
Ran for 5550 frames Cell Events: 206 mean_circ: 0.95838
Ran for 6050 frames Cell Events: 223 mean_circ: 0.958822
Ran for 6550 frames Cell Events: 239 mean_circ: 0.959246
Ran for 7050 frames Cell Events: 253 mean_circ: 0.95909
Ran for 7550 frames Cell Events: 273 mean_circ: 0.958591
Ran for 8550 frames Cell Events: 309 mean_circ: 0.958858
Ran for 9050 frames Cell Events: 323 mean_circ: 0.958818
Ran for 9550 frames Cell Events: 342 mean_circ: 0.958547
Ran for 10050 frames Cell Events: 370 mean_circ: 0.959443
Ran for 10550 frames Cell Events: 380 mean_circ: 0.959691
Ran for 11050 frames Cell Events: 394 mean_circ: 0.959294
Ran for 11550 frames Cell Events: 405 mean_circ: 0.958929
Ran for 12550 frames Cell Events: 445 mean_circ: 0.958869
Ran for 13050 frames Cell Events: 460 mean_circ: 0.958672
Ran for 13550 frames Cell Events: 475 mean_circ: 0.958673
Ran for 15550 frames Cell Events: 538 mean_circ: 0.958078
Ran for 16050 frames Cell Events: 553 mean_circ: 0.958353
Ran for 16550 frames Cell Events: 562 mean_circ: 0.958118
Ran for 17050 frames Cell Events: 577 mean_circ: 0.958165
Ran for 18050 frames Cell Events: 612 mean_circ: 0.957762
Ran for 18550 frames Cell Events: 633 mean_circ: 0.957357
Ran for 19050 frames Cell Events: 655 mean_circ: 0.957027
Ran for 19550 frames Cell Events: 673 mean_circ: 0.957187
Ran for 20050 frames Cell Events: 691 mean_circ: 0.957174
Ran for 20550 frames Cell Events: 704 mean_circ: 0.957475
Ran for 21050 frames Cell Events: 719 mean_circ: 0.95754
Ran for 21550 frames Cell Events: 738 mean_circ: 0.957367
Ran for 22550 frames Cell Events: 764 mean_circ: 0.957406
Ran for 23050 frames Cell Events: 779 mean_circ: 0.957337
Ran for 23550 frames Cell Events: 801 mean_circ: 0.957649
Ran for 24050 frames Cell Events: 816 mean_circ: 0.957662
Ran for 24550 frames Cell Events: 829 mean_circ: 0.957399
Ran for 25550 frames Cell Events: 871 mean_circ: 0.957089
Ran for 26050 frames Cell Events: 886 mean_circ: 0.957311
Ran for 26550 frames Cell Events: 901 mean_circ: 0.957286
Ran for 27050 frames Cell Events: 910 mean_circ: 0.957335
Ran for 27550 frames Cell Events: 929 mean_circ: 0.957191
Ran for 28050 frames Cell Events: 944 mean_circ: 0.957275
Ran for 28550 frames Cell Events: 974 mean_circ: 0.957251
Ran for 29050 frames Cell Events: 993 mean_circ: 0.956964
Ran for 30050 frames Cell Events: 1024 mean_circ: 0.956875
Ran for 30550 frames Cell Events: 1034 mean_circ: 0.957008
Ran for 31550 frames Cell Events: 1069 mean_circ: 0.95695
Ran for 32050 frames Cell Events: 1089 mean_circ: 0.95707
Ran for 32550 frames Cell Events: 1099 mean_circ: 0.957153
Ran for 33050 frames Cell Events: 1109 mean_circ: 0.957201
Ran for 33550 frames Cell Events: 1122 mean_circ: 0.956836
Ran for 34550 frames Cell Events: 1155 mean_circ: 0.956991
Ran for 35050 frames Cell Events: 1177 mean_circ: 0.957156
Ran for 35550 frames Cell Events: 1189 mean_circ: 0.957074
Ran for 36050 frames Cell Events: 1200 mean_circ: 0.957042
Ran for 36550 frames Cell Events: 1210 mean_circ: 0.95695
Ran for 37050 frames Cell Events: 1225 mean_circ: 0.956853
Ran for 37550 frames Cell Events: 1239 mean_circ: 0.956943
Ran for 38550 frames Cell Events: 1265 mean_circ: 0.956795
Ran for 39050 frames Cell Events: 1285 mean_circ: 0.956804
Ran for 39550 frames Cell Events: 1302 mean_circ: 0.956845
Ran for 40050 frames Cell Events: 1314 mean_circ: 0.956929
Ran for 40550 frames Cell Events: 1329 mean_circ: 0.956983
Ran for 41550 frames Cell Events: 1358 mean_circ: 0.95684
Ran for 42550 frames Cell Events: 1393 mean_circ: 0.95696
Ran for 43050 frames Cell Events: 1411 mean_circ: 0.957086
Ran for 44550 frames Cell Events: 1457 mean_circ: 0.957073
Ran for 45050 frames Cell Events: 1473 mean_circ: 0.957106
Ran for 45550 frames Cell Events: 1479 mean_circ: 0.957114
Ran for 46050 frames Cell Events: 1498 mean_circ: 0.957135
Ran for 47050 frames Cell Events: 1531 mean_circ: 0.957134
Ran for 49050 frames Cell Events: 1616 mean_circ: 0.956783
Ran for 49550 frames Cell Events: 1640 mean_circ: 0.956617
Ran for 50050 frames Cell Events: 1651 mean_circ: 0.956635
Ran for 50550 frames Cell Events: 1660 mean_circ: 0.956679
Ran for 51550 frames Cell Events: 1683 mean_circ: 0.956729
Ran for 52050 frames Cell Events: 1694 mean_circ: 0.956678
Ran for 52550 frames Cell Events: 1716 mean_circ: 0.956603
Ran for 54050 frames Cell Events: 1771 mean_circ: 0.956788
Ran for 55050 frames Cell Events: 1798 mean_circ: 0.956949
Ran for 55550 frames Cell Events: 1809 mean_circ: 0.956936
Ran for 56050 frames Cell Events: 1821 mean_circ: 0.956991
Ran for 56550 frames Cell Events: 1830 mean_circ: 0.956945
Ran for 57050 frames Cell Events: 1847 mean_circ: 0.956911
Ran for 58050 frames Cell Events: 1869 mean_circ: 0.956929
Ran for 58550 frames Cell Events: 1883 mean_circ: 0.956967
Ran for 59050 frames Cell Events: 1902 mean_circ: 0.956893
Ran for 60050 frames Cell Events: 1953 mean_circ: 0.956948
Ran for 60550 frames Cell Events: 1973 mean_circ: 0.95683
Ran for 61550 frames Cell Events: 2014 mean_circ: 0.956749
Ran for 62050 frames Cell Events: 2026 mean_circ: 0.956807
Ran for 62550 frames Cell Events: 2060 mean_circ: 0.956809
Ran for 64050 frames Cell Events: 2122 mean_circ: 0.956792
Ran for 64550 frames Cell Events: 2139 mean_circ: 0.956822
Ran for 65050 frames Cell Events: 2156 mean_circ: 0.956889
Ran for 65550 frames Cell Events: 2170 mean_circ: 0.956885
Ran for 66050 frames Cell Events: 2184 mean_circ: 0.956968
Ran for 67550 frames Cell Events: 2231 mean_circ: 0.957063
Ran for 68550 frames Cell Events: 2266 mean_circ: 0.957061
Ran for 69050 frames Cell Events: 2295 mean_circ: 0.957172
Ran for 70550 frames Cell Events: 2350 mean_circ: 0.957017
Ran for 72050 frames Cell Events: 2406 mean_circ: 0.956983
Ran for 72550 frames Cell Events: 2429 mean_circ: 0.95693
Ran for 74050 frames Cell Events: 2483 mean_circ: 0.956829
Ran for 74550 frames Cell Events: 2500 mean_circ: 0.95682
Ran for 75050 frames Cell Events: 2525 mean_circ: 0.956785
Ran for 76050 frames Cell Events: 2572 mean_circ: 0.956806
Ran for 77050 frames Cell Events: 2629 mean_circ: 0.956977
Ran for 79550 frames Cell Events: 2774 mean_circ: 0.956384
Ran for 81050 frames Cell Events: 2858 mean_circ: 0.956255
Ran for 82050 frames Cell Events: 2891 mean_circ: 0.956353
Ran for 82550 frames Cell Events: 2924 mean_circ: 0.955919
Ran for 84050 frames Cell Events: 2969 mean_circ: 0.956046
Ran for 85050 frames Cell Events: 3004 mean_circ: 0.956078
Ran for 85550 frames Cell Events: 3016 mean_circ: 0.956007
Ran for 86050 frames Cell Events: 3038 mean_circ: 0.956041
Ran for 86550 frames Cell Events: 3056 mean_circ: 0.956033
Ran for 87050 frames Cell Events: 3083 mean_circ: 0.956057
Ran for 88550 frames Cell Events: 3149 mean_circ: 0.956011
Ran for 89550 frames Cell Events: 3188 mean_circ: 0.955991
Ran for 90550 frames Cell Events: 3217 mean_circ: 0.956
Ran for 91050 frames Cell Events: 3242 mean_circ: 0.955903
Ran for 91550 frames Cell Events: 3260 mean_circ: 0.955888
Ran for 92550 frames Cell Events: 3295 mean_circ: 0.955892
Ran for 93050 frames Cell Events: 3316 mean_circ: 0.955892
Ran for 93550 frames Cell Events: 3338 mean_circ: 0.955816
Ran for 94550 frames Cell Events: 3363 mean_circ: 0.955806
Ran for 95050 frames Cell Events: 3386 mean_circ: 0.955714
Ran for 95550 frames Cell Events: 3412 mean_circ: 0.955714
Ran for 96050 frames Cell Events: 3432 mean_circ: 0.955725
Ran for 97550 frames Cell Events: 3499 mean_circ: 0.955653
Ran for 98050 frames Cell Events: 3519 mean_circ: 0.955662
Ran for 98550 frames Cell Events: 3548 mean_circ: 0.955723
Ran for 99050 frames Cell Events: 3572 mean_circ: 0.955787
Ran for 99550 frames Cell Events: 3598 mean_circ: 0.95581
Ran for 100550 frames Cell Events: 3633 mean_circ: 0.955825
Ran for 101050 frames Cell Events: 3661 mean_circ: 0.955619
Ran for 101550 frames Cell Events: 3693 mean_circ: 0.955677
Ran for 102050 frames Cell Events: 3705 mean_circ: 0.955662
Ran for 103050 frames Cell Events: 3738 mean_circ: 0.955691
Ran for 104050 frames Cell Events: 3771 mean_circ: 0.955699
Ran for 104550 frames Cell Events: 3790 mean_circ: 0.955701
Ran for 105050 frames Cell Events: 3809 mean_circ: 0.955734
Ran for 105550 frames Cell Events: 3831 mean_circ: 0.955811
Ran for 106050 frames Cell Events: 3853 mean_circ: 0.955786
Ran for 106550 frames Cell Events: 3871 mean_circ: 0.955811
Ran for 107050 frames Cell Events: 3887 mean_circ: 0.955792
Ran for 107550 frames Cell Events: 3906 mean_circ: 0.955817
Ran for 108550 frames Cell Events: 3947 mean_circ: 0.955861
Ran for 109050 frames Cell Events: 3960 mean_circ: 0.955863
Ran for 109550 frames Cell Events: 3980 mean_circ: 0.955755
Ran for 110050 frames Cell Events: 3991 mean_circ: 0.955759
Ran for 110550 frames Cell Events: 4010 mean_circ: 0.955786
Ran for 112050 frames Cell Events: 4067 mean_circ: 0.955938
Ran for 115050 frames Cell Events: 4185 mean_circ: 0.955977
Ran for 116050 frames Cell Events: 4220 mean_circ: 0.956004
Ran for 116550 frames Cell Events: 4243 mean_circ: 0.956047
Ran for 117050 frames Cell Events: 4258 mean_circ: 0.956052
Ran for 117550 frames Cell Events: 4282 mean_circ: 0.956036
Ran for 118050 frames Cell Events: 4300 mean_circ: 0.956086
Ran for 118550 frames Cell Events: 4325 mean_circ: 0.956112
Ran for 119050 frames Cell Events: 4346 mean_circ: 0.95617
Ran for 119550 frames Cell Events: 4371 mean_circ: 0.956156
Ran for 120050 frames Cell Events: 4383 mean_circ: 0.956155
Ran for 121550 frames Cell Events: 4432 mean_circ: 0.956135
Ran for 122050 frames Cell Events: 4445 mean_circ: 0.956145
Ran for 123050 frames Cell Events: 4481 mean_circ: 0.956164
Ran for 124050 frames Cell Events: 4506 mean_circ: 0.956201
Ran for 125050 frames Cell Events: 4556 mean_circ: 0.956158
Ran for 127050 frames Cell Events: 4653 mean_circ: 0.956232
Ran for 127550 frames Cell Events: 4674 mean_circ: 0.956251
Ran for 128050 frames Cell Events: 4694 mean_circ: 0.956295
Ran for 128550 frames Cell Events: 4713 mean_circ: 0.956299
Ran for 129050 frames Cell Events: 4732 mean_circ: 0.956303
Ran for 129550 frames Cell Events: 4753 mean_circ: 0.956285
Ran for 130550 frames Cell Events: 4785 mean_circ: 0.95625
Ran for 131050 frames Cell Events: 4807 mean_circ: 0.956293
Ran for 131550 frames Cell Events: 4817 mean_circ: 0.956326
Ran for 132550 frames Cell Events: 4857 mean_circ: 0.956329
Ran for 133050 frames Cell Events: 4867 mean_circ: 0.956334
Ran for 133550 frames Cell Events: 4879 mean_circ: 0.956358
Ran for 134050 frames Cell Events: 4898 mean_circ: 0.95635
Ran for 134550 frames Cell Events: 4918 mean_circ: 0.956345
Ran for 136050 frames Cell Events: 4975 mean_circ: 0.9564
Ran for 136550 frames Cell Events: 4989 mean_circ: 0.956419
Ran for 137550 frames Cell Events: 5040 mean_circ: 0.956314
Ran for 138550 frames Cell Events: 5089 mean_circ: 0.956332
Ran for 140050 frames Cell Events: 5167 mean_circ: 0.956353
Ran for 141550 frames Cell Events: 5238 mean_circ: 0.956459
Ran for 142050 frames Cell Events: 5265 mean_circ: 0.956478
Ran for 142550 frames Cell Events: 5294 mean_circ: 0.956366
Ran for 143050 frames Cell Events: 5314 mean_circ: 0.956346
Ran for 143550 frames Cell Events: 5342 mean_circ: 0.95632
Ran for 144050 frames Cell Events: 5356 mean_circ: 0.956319
Ran for 145050 frames Cell Events: 5413 mean_circ: 0.956341
Ran for 145550 frames Cell Events: 5432 mean_circ: 0.956367
Ran for 146050 frames Cell Events: 5449 mean_circ: 0.956378
Ran for 147550 frames Cell Events: 5506 mean_circ: 0.956424
Ran for 148050 frames Cell Events: 5517 mean_circ: 0.956407
Ran for 149050 frames Cell Events: 5523 mean_circ: 0.956268
Ran for 149550 frames Cell Events: 5523 mean_circ: 0.956268
Ran for 150550 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 151550 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 152050 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 152550 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 153050 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 153550 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 154050 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 154550 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 155050 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 155550 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 156050 frames Cell Events: 5529 mean_circ: 0.956117
Ran for 156550 frames Cell Events: 5529 mean_circ: 0.956117



End this measurment..



Stopping time: 2.90264e+008
****************************************
Measurement finished

Mean Circularitiy: 0.956117
Mean Area: 620.706
Mean FL-1 intensity: 2871.71
Mean FL-2 intensity: 4469.22
Mean FL-3 intensity: 0

frames processed: 156347
Total Time: 78486.7ms
Number of Runs (new bg img) 0
Frames collected: 156790
Skipped frames: 52203
Rescued Frames: 2810641
Samples DAQ: 39197435
Samples written: 78394798
Samples per Channel: 39197399
FPS: 1997.66

Samples per second: 499415
Expected samples/s: 500000

Events: 5529
Events per second: 70.4451
jumped back (PMT): 3157
#Frames read, frequency
# 0 frames :217825
# 1 frames :84964
# 2 frames :2849
# 3 frames :1108
# 4 frames :575
# 5 frames :257
# 6 frames :1288
# 7 frames :1118
# 8 frames :673
# 9 frames :339
# 10 frames :756
# 11 frames :391
# 12 frames :299
# 13 frames :141
# 14 frames :172
# 15 frames :136
# 16 frames :163
# 17 frames :134
# 18 frames :87
# 19 frames :42
# 20 frames :31
# 21 frames :32
# 22 frames :25
# 23 frames :24
# 24 frames :26
# 25 frames :19
# 26 frames :6
# 27 frames :10
# 28 frames :9
# 29 frames :7
# 30 frames :8
# 31 frames :5
# 32 frames :2
# 33 frames :4
# 34 frames :2
# 35 frames :1
# 36 frames :2
# 37 frames :2
# 38 frames :3
# 39 frames :1
# 40 frames :1
# 41 frames :1
# 42 frames :2
# 43 frames :1
# 45 frames :2
# 47 frames :2
# 48 frames :1
# 51 frames :1
# 52 frames :1
# 54 frames :2
# 68 frames :1
# 72 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.3913
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0