Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.05.20 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "996b396fd79d6f5c49b3724413595400",
"name": "M1_20170518_1819_dsRNA_I7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:05:45"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8095 (max 8096) in '8095'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.3929
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.94993e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 37 mean_circ: 0.903517
Ran for 1050 frames Cell Events: 58 mean_circ: 0.944064
Ran for 2050 frames Cell Events: 119 mean_circ: 0.946504
Ran for 2550 frames Cell Events: 150 mean_circ: 0.946504
Ran for 3050 frames Cell Events: 171 mean_circ: 0.9486
Ran for 3550 frames Cell Events: 196 mean_circ: 0.94904
Ran for 4050 frames Cell Events: 207 mean_circ: 0.949061
Ran for 4550 frames Cell Events: 233 mean_circ: 0.949374
Ran for 6050 frames Cell Events: 293 mean_circ: 0.95097
Ran for 6550 frames Cell Events: 319 mean_circ: 0.951567
Ran for 7050 frames Cell Events: 350 mean_circ: 0.952323
Ran for 7550 frames Cell Events: 370 mean_circ: 0.952169
Ran for 8050 frames Cell Events: 406 mean_circ: 0.952427
Ran for 9050 frames Cell Events: 464 mean_circ: 0.952226
Ran for 9550 frames Cell Events: 488 mean_circ: 0.952299
Ran for 10550 frames Cell Events: 552 mean_circ: 0.952687
Ran for 11050 frames Cell Events: 573 mean_circ: 0.952998
Ran for 11550 frames Cell Events: 595 mean_circ: 0.952701
Ran for 12550 frames Cell Events: 641 mean_circ: 0.952609
Ran for 13550 frames Cell Events: 682 mean_circ: 0.952974
Ran for 14550 frames Cell Events: 753 mean_circ: 0.953045
Ran for 15550 frames Cell Events: 808 mean_circ: 0.953296
Ran for 16050 frames Cell Events: 836 mean_circ: 0.953113
Ran for 18050 frames Cell Events: 954 mean_circ: 0.953108
Ran for 19050 frames Cell Events: 1017 mean_circ: 0.953124
Ran for 19550 frames Cell Events: 1042 mean_circ: 0.953319
Ran for 21050 frames Cell Events: 1113 mean_circ: 0.953173
Ran for 22050 frames Cell Events: 1165 mean_circ: 0.953586
Ran for 23050 frames Cell Events: 1244 mean_circ: 0.953507
Ran for 23550 frames Cell Events: 1262 mean_circ: 0.953213
Ran for 24050 frames Cell Events: 1277 mean_circ: 0.953252
Ran for 25550 frames Cell Events: 1356 mean_circ: 0.953438
Ran for 26050 frames Cell Events: 1382 mean_circ: 0.953371
Ran for 27550 frames Cell Events: 1483 mean_circ: 0.953596
Ran for 28550 frames Cell Events: 1527 mean_circ: 0.953578
Ran for 30050 frames Cell Events: 1618 mean_circ: 0.953502
Ran for 32050 frames Cell Events: 1711 mean_circ: 0.953367
Ran for 33050 frames Cell Events: 1763 mean_circ: 0.953654
Ran for 34050 frames Cell Events: 1818 mean_circ: 0.953769
Ran for 34550 frames Cell Events: 1838 mean_circ: 0.953773
Ran for 35550 frames Cell Events: 1892 mean_circ: 0.953764
Ran for 36050 frames Cell Events: 1925 mean_circ: 0.953689
Ran for 36550 frames Cell Events: 1944 mean_circ: 0.953737
Ran for 37050 frames Cell Events: 1975 mean_circ: 0.953692
Ran for 37550 frames Cell Events: 1996 mean_circ: 0.953592
Ran for 41050 frames Cell Events: 2192 mean_circ: 0.953155
Ran for 43050 frames Cell Events: 2325 mean_circ: 0.953514
Ran for 43550 frames Cell Events: 2349 mean_circ: 0.9535
Ran for 44550 frames Cell Events: 2413 mean_circ: 0.953528
Ran for 46050 frames Cell Events: 2496 mean_circ: 0.95363
Ran for 47050 frames Cell Events: 2564 mean_circ: 0.953565
Ran for 49050 frames Cell Events: 2655 mean_circ: 0.953404
Ran for 51050 frames Cell Events: 2760 mean_circ: 0.953293
Ran for 51550 frames Cell Events: 2790 mean_circ: 0.953099
Ran for 52550 frames Cell Events: 2854 mean_circ: 0.953157
Ran for 53050 frames Cell Events: 2886 mean_circ: 0.953163
Ran for 54050 frames Cell Events: 2954 mean_circ: 0.953053
Ran for 54550 frames Cell Events: 2979 mean_circ: 0.953046
Ran for 55050 frames Cell Events: 3007 mean_circ: 0.953178
Ran for 57050 frames Cell Events: 3116 mean_circ: 0.953219
Ran for 58050 frames Cell Events: 3180 mean_circ: 0.953189
Ran for 58550 frames Cell Events: 3221 mean_circ: 0.953231
Ran for 59550 frames Cell Events: 3283 mean_circ: 0.953288
Ran for 60050 frames Cell Events: 3303 mean_circ: 0.953311
Ran for 60550 frames Cell Events: 3339 mean_circ: 0.953354
Ran for 61050 frames Cell Events: 3365 mean_circ: 0.953455
Ran for 62050 frames Cell Events: 3419 mean_circ: 0.953495
Ran for 62550 frames Cell Events: 3450 mean_circ: 0.953459
Ran for 63050 frames Cell Events: 3474 mean_circ: 0.953361
Ran for 63550 frames Cell Events: 3507 mean_circ: 0.953404
Ran for 64550 frames Cell Events: 3569 mean_circ: 0.953496
Ran for 65050 frames Cell Events: 3598 mean_circ: 0.95345
Ran for 66050 frames Cell Events: 3642 mean_circ: 0.953515
Ran for 66550 frames Cell Events: 3658 mean_circ: 0.953496
Ran for 67050 frames Cell Events: 3682 mean_circ: 0.953494
Ran for 70550 frames Cell Events: 3887 mean_circ: 0.953623
Ran for 71550 frames Cell Events: 3926 mean_circ: 0.953548
Ran for 72050 frames Cell Events: 3955 mean_circ: 0.953553
Ran for 72550 frames Cell Events: 3983 mean_circ: 0.953538
Ran for 73050 frames Cell Events: 4007 mean_circ: 0.953474
Ran for 74550 frames Cell Events: 4060 mean_circ: 0.953438
Ran for 75050 frames Cell Events: 4083 mean_circ: 0.953414
Ran for 76050 frames Cell Events: 4130 mean_circ: 0.95344
Ran for 76550 frames Cell Events: 4162 mean_circ: 0.953382
Ran for 77050 frames Cell Events: 4186 mean_circ: 0.953359
Ran for 77550 frames Cell Events: 4221 mean_circ: 0.953294
Ran for 78550 frames Cell Events: 4287 mean_circ: 0.953357
Ran for 79050 frames Cell Events: 4322 mean_circ: 0.953349
Ran for 80550 frames Cell Events: 4359 mean_circ: 0.953292
Ran for 81550 frames Cell Events: 4404 mean_circ: 0.953397
Ran for 82050 frames Cell Events: 4432 mean_circ: 0.953354
Ran for 83050 frames Cell Events: 4494 mean_circ: 0.953275
Ran for 83550 frames Cell Events: 4520 mean_circ: 0.953308
Ran for 85550 frames Cell Events: 4634 mean_circ: 0.953255
Ran for 86050 frames Cell Events: 4657 mean_circ: 0.953293
Ran for 87050 frames Cell Events: 4703 mean_circ: 0.953324
Ran for 87550 frames Cell Events: 4729 mean_circ: 0.95335
Ran for 90050 frames Cell Events: 4881 mean_circ: 0.953357
Ran for 90550 frames Cell Events: 4906 mean_circ: 0.953394
Ran for 91550 frames Cell Events: 4987 mean_circ: 0.95329
Ran for 92550 frames Cell Events: 5070 mean_circ: 0.953256
Ran for 93550 frames Cell Events: 5146 mean_circ: 0.95308
Ran for 94550 frames Cell Events: 5207 mean_circ: 0.953185
Ran for 95050 frames Cell Events: 5251 mean_circ: 0.95315
Ran for 95550 frames Cell Events: 5275 mean_circ: 0.953126
Ran for 97550 frames Cell Events: 5408 mean_circ: 0.952965
Ran for 99050 frames Cell Events: 5490 mean_circ: 0.95289
Ran for 100050 frames Cell Events: 5534 mean_circ: 0.952918
Ran for 100550 frames Cell Events: 5547 mean_circ: 0.952866
Ran for 101550 frames Cell Events: 5603 mean_circ: 0.952772
Ran for 102550 frames Cell Events: 5650 mean_circ: 0.952838
Ran for 103050 frames Cell Events: 5677 mean_circ: 0.952817
Ran for 103550 frames Cell Events: 5708 mean_circ: 0.952705
Ran for 104050 frames Cell Events: 5737 mean_circ: 0.952683
Ran for 104550 frames Cell Events: 5773 mean_circ: 0.95271
Ran for 106050 frames Cell Events: 5864 mean_circ: 0.952657
Ran for 106550 frames Cell Events: 5887 mean_circ: 0.952762
Ran for 107050 frames Cell Events: 5910 mean_circ: 0.952778
Ran for 108550 frames Cell Events: 5979 mean_circ: 0.952745
Ran for 109550 frames Cell Events: 6028 mean_circ: 0.95278
Ran for 110050 frames Cell Events: 6046 mean_circ: 0.952769
Ran for 110550 frames Cell Events: 6078 mean_circ: 0.952821
Ran for 111050 frames Cell Events: 6110 mean_circ: 0.952836
Ran for 112050 frames Cell Events: 6194 mean_circ: 0.952882
Ran for 112550 frames Cell Events: 6232 mean_circ: 0.952856
Ran for 113550 frames Cell Events: 6273 mean_circ: 0.95287
Ran for 114050 frames Cell Events: 6302 mean_circ: 0.952898
Ran for 114550 frames Cell Events: 6326 mean_circ: 0.952893
Ran for 116050 frames Cell Events: 6409 mean_circ: 0.952928
Ran for 116550 frames Cell Events: 6433 mean_circ: 0.952894
Ran for 117550 frames Cell Events: 6493 mean_circ: 0.952861
Ran for 121550 frames Cell Events: 6783 mean_circ: 0.952824
Ran for 122050 frames Cell Events: 6803 mean_circ: 0.952844
Ran for 123550 frames Cell Events: 6921 mean_circ: 0.952806
Ran for 124050 frames Cell Events: 6957 mean_circ: 0.952822
Ran for 124550 frames Cell Events: 6991 mean_circ: 0.952819
Ran for 125050 frames Cell Events: 7015 mean_circ: 0.952803
Ran for 125550 frames Cell Events: 7056 mean_circ: 0.952819
Ran for 127050 frames Cell Events: 7144 mean_circ: 0.952885
Ran for 127550 frames Cell Events: 7178 mean_circ: 0.952879
Ran for 128550 frames Cell Events: 7250 mean_circ: 0.952967
Ran for 130550 frames Cell Events: 7406 mean_circ: 0.953021
Ran for 134050 frames Cell Events: 7634 mean_circ: 0.952952
Ran for 135550 frames Cell Events: 7732 mean_circ: 0.952994
Ran for 136550 frames Cell Events: 7793 mean_circ: 0.953025
Ran for 137550 frames Cell Events: 7861 mean_circ: 0.953014
Ran for 138050 frames Cell Events: 7885 mean_circ: 0.953045
Ran for 141050 frames Cell Events: 8062 mean_circ: 0.953023
Ran for 141550 frames Cell Events: 8089 mean_circ: 0.952992



End this measurment..



Stopping time: 2.95064e+008
****************************************
Measurement finished

Mean Circularitiy: 0.953009
Mean Area: 597.189
Mean FL-1 intensity: 3748.43
Mean FL-2 intensity: 5047.38
Mean FL-3 intensity: 0

frames processed: 139685
Total Time: 70952ms
Number of Runs (new bg img) 0
Frames collected: 141756
Skipped frames: 70468
Rescued Frames: 3937819
Samples DAQ: 35440455
Samples written: 70879468
Samples per Channel: 35439734
FPS: 1997.91

Samples per second: 499489
Expected samples/s: 500000

Events: 8096
Events per second: 114.105
jumped back (PMT): 3356
#Frames read, frequency
# 0 frames :215879
# 1 frames :55996
# 2 frames :2918
# 3 frames :890
# 4 frames :490
# 5 frames :218
# 6 frames :1671
# 7 frames :1473
# 8 frames :970
# 9 frames :471
# 10 frames :433
# 11 frames :287
# 12 frames :244
# 13 frames :163
# 14 frames :215
# 15 frames :203
# 16 frames :156
# 17 frames :115
# 18 frames :84
# 19 frames :59
# 20 frames :60
# 21 frames :82
# 22 frames :63
# 23 frames :46
# 24 frames :29
# 25 frames :39
# 26 frames :27
# 27 frames :25
# 28 frames :19
# 29 frames :14
# 30 frames :22
# 31 frames :14
# 32 frames :12
# 33 frames :13
# 34 frames :12
# 35 frames :5
# 36 frames :5
# 37 frames :5
# 38 frames :5
# 39 frames :1
# 40 frames :4
# 41 frames :5
# 42 frames :5
# 43 frames :2
# 44 frames :2
# 45 frames :5
# 46 frames :5
# 47 frames :3
# 48 frames :2
# 49 frames :3
# 50 frames :2
# 52 frames :1
# 55 frames :2
# 56 frames :1
# 57 frames :1
# 58 frames :1
# 60 frames :1
# 62 frames :1
# 65 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.3929
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0