Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.05.54 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "14e77fd6f08682b411b8bdd815fc5fd7",
"name": "M1_20170518_1821_dsRNA_I7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:06:20"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8118 (max 8119) in '8118'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.398
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.95155e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 78 mean_circ: 0.94597
Ran for 2550 frames Cell Events: 130 mean_circ: 0.947483
Ran for 3050 frames Cell Events: 162 mean_circ: 0.94741
Ran for 4050 frames Cell Events: 224 mean_circ: 0.947039
Ran for 4550 frames Cell Events: 266 mean_circ: 0.947428
Ran for 5050 frames Cell Events: 303 mean_circ: 0.946917
Ran for 6050 frames Cell Events: 360 mean_circ: 0.945807
Ran for 7050 frames Cell Events: 427 mean_circ: 0.944197
Ran for 7550 frames Cell Events: 455 mean_circ: 0.943858
Ran for 8550 frames Cell Events: 520 mean_circ: 0.943621
Ran for 9050 frames Cell Events: 556 mean_circ: 0.943426
Ran for 9550 frames Cell Events: 595 mean_circ: 0.942936
Ran for 10050 frames Cell Events: 639 mean_circ: 0.942787
Ran for 11050 frames Cell Events: 687 mean_circ: 0.943477
Ran for 12050 frames Cell Events: 739 mean_circ: 0.943293
Ran for 12550 frames Cell Events: 767 mean_circ: 0.943142
Ran for 13050 frames Cell Events: 797 mean_circ: 0.943418
Ran for 14050 frames Cell Events: 846 mean_circ: 0.9434
Ran for 15550 frames Cell Events: 954 mean_circ: 0.943706
Ran for 18550 frames Cell Events: 1122 mean_circ: 0.944108
Ran for 19050 frames Cell Events: 1152 mean_circ: 0.944078
Ran for 19550 frames Cell Events: 1195 mean_circ: 0.943606
Ran for 20550 frames Cell Events: 1251 mean_circ: 0.943413
Ran for 21050 frames Cell Events: 1271 mean_circ: 0.943338
Ran for 23550 frames Cell Events: 1415 mean_circ: 0.943264
Ran for 29550 frames Cell Events: 1886 mean_circ: 0.943365
Ran for 32050 frames Cell Events: 2047 mean_circ: 0.943832
Ran for 32550 frames Cell Events: 2070 mean_circ: 0.943794
Ran for 33050 frames Cell Events: 2091 mean_circ: 0.943783
Ran for 33550 frames Cell Events: 2126 mean_circ: 0.943851
Ran for 34050 frames Cell Events: 2148 mean_circ: 0.943882
Ran for 35050 frames Cell Events: 2215 mean_circ: 0.943787
Ran for 36050 frames Cell Events: 2278 mean_circ: 0.943784
Ran for 36550 frames Cell Events: 2329 mean_circ: 0.943784
Ran for 37050 frames Cell Events: 2365 mean_circ: 0.943811
Ran for 38050 frames Cell Events: 2422 mean_circ: 0.943689
Ran for 38550 frames Cell Events: 2450 mean_circ: 0.943718
Ran for 39050 frames Cell Events: 2486 mean_circ: 0.943675
Ran for 39550 frames Cell Events: 2515 mean_circ: 0.943675
Ran for 40050 frames Cell Events: 2533 mean_circ: 0.943394
Ran for 40550 frames Cell Events: 2559 mean_circ: 0.943286
Ran for 41050 frames Cell Events: 2593 mean_circ: 0.943286
Ran for 42050 frames Cell Events: 2642 mean_circ: 0.943489
Ran for 42550 frames Cell Events: 2669 mean_circ: 0.943544
Ran for 44050 frames Cell Events: 2762 mean_circ: 0.943694
Ran for 45550 frames Cell Events: 2848 mean_circ: 0.943704
Ran for 46050 frames Cell Events: 2874 mean_circ: 0.943813
Ran for 47050 frames Cell Events: 2945 mean_circ: 0.943798
Ran for 48050 frames Cell Events: 3009 mean_circ: 0.943661
Ran for 50550 frames Cell Events: 3177 mean_circ: 0.943562
Ran for 52550 frames Cell Events: 3322 mean_circ: 0.94368
Ran for 53050 frames Cell Events: 3351 mean_circ: 0.943845
Ran for 54550 frames Cell Events: 3442 mean_circ: 0.943809
Ran for 55050 frames Cell Events: 3468 mean_circ: 0.943928
Ran for 55550 frames Cell Events: 3502 mean_circ: 0.943986
Ran for 56050 frames Cell Events: 3528 mean_circ: 0.944089
Ran for 56550 frames Cell Events: 3552 mean_circ: 0.944169
Ran for 59050 frames Cell Events: 3698 mean_circ: 0.944257
Ran for 60050 frames Cell Events: 3762 mean_circ: 0.944229
Ran for 61050 frames Cell Events: 3833 mean_circ: 0.944178
Ran for 61550 frames Cell Events: 3856 mean_circ: 0.94422
Ran for 63050 frames Cell Events: 3949 mean_circ: 0.94433
Ran for 64550 frames Cell Events: 4023 mean_circ: 0.944405
Ran for 65050 frames Cell Events: 4044 mean_circ: 0.944387
Ran for 65550 frames Cell Events: 4081 mean_circ: 0.944388
Ran for 67550 frames Cell Events: 4189 mean_circ: 0.944548
Ran for 68550 frames Cell Events: 4272 mean_circ: 0.944551
Ran for 69550 frames Cell Events: 4341 mean_circ: 0.944388
Ran for 73550 frames Cell Events: 4616 mean_circ: 0.944336
Ran for 74550 frames Cell Events: 4683 mean_circ: 0.944426
Ran for 75550 frames Cell Events: 4742 mean_circ: 0.944427
Ran for 77050 frames Cell Events: 4845 mean_circ: 0.944462
Ran for 78550 frames Cell Events: 4923 mean_circ: 0.944364
Ran for 79050 frames Cell Events: 4948 mean_circ: 0.944413
Ran for 79550 frames Cell Events: 4988 mean_circ: 0.944284
Ran for 81050 frames Cell Events: 5066 mean_circ: 0.944151
Ran for 82050 frames Cell Events: 5135 mean_circ: 0.944111
Ran for 83050 frames Cell Events: 5205 mean_circ: 0.944136
Ran for 83550 frames Cell Events: 5234 mean_circ: 0.944136
Ran for 84050 frames Cell Events: 5257 mean_circ: 0.94409
Ran for 85550 frames Cell Events: 5377 mean_circ: 0.944027
Ran for 86550 frames Cell Events: 5430 mean_circ: 0.944061
Ran for 87050 frames Cell Events: 5459 mean_circ: 0.944032
Ran for 87550 frames Cell Events: 5506 mean_circ: 0.943979
Ran for 88050 frames Cell Events: 5531 mean_circ: 0.943988
Ran for 90050 frames Cell Events: 5679 mean_circ: 0.943919
Ran for 90550 frames Cell Events: 5713 mean_circ: 0.943974
Ran for 91050 frames Cell Events: 5736 mean_circ: 0.943931
Ran for 91550 frames Cell Events: 5770 mean_circ: 0.943921
Ran for 92050 frames Cell Events: 5796 mean_circ: 0.94391
Ran for 93550 frames Cell Events: 5893 mean_circ: 0.943953
Ran for 94050 frames Cell Events: 5929 mean_circ: 0.943991
Ran for 94550 frames Cell Events: 5964 mean_circ: 0.944026
Ran for 96050 frames Cell Events: 6058 mean_circ: 0.943867
Ran for 99550 frames Cell Events: 6273 mean_circ: 0.943799
Ran for 102550 frames Cell Events: 6484 mean_circ: 0.943896
Ran for 103550 frames Cell Events: 6547 mean_circ: 0.943957
Ran for 105550 frames Cell Events: 6671 mean_circ: 0.943972
Ran for 106050 frames Cell Events: 6694 mean_circ: 0.943967
Ran for 106550 frames Cell Events: 6727 mean_circ: 0.943939
Ran for 109050 frames Cell Events: 6900 mean_circ: 0.943753
Ran for 110550 frames Cell Events: 6992 mean_circ: 0.943798
Ran for 111550 frames Cell Events: 7061 mean_circ: 0.943777
Ran for 113050 frames Cell Events: 7178 mean_circ: 0.94378
Ran for 114550 frames Cell Events: 7291 mean_circ: 0.943831
Ran for 116050 frames Cell Events: 7381 mean_circ: 0.943908
Ran for 117050 frames Cell Events: 7438 mean_circ: 0.943961
Ran for 118050 frames Cell Events: 7493 mean_circ: 0.943952
Ran for 119550 frames Cell Events: 7576 mean_circ: 0.944018
Ran for 120050 frames Cell Events: 7607 mean_circ: 0.943973
Ran for 120550 frames Cell Events: 7629 mean_circ: 0.944014
Ran for 122050 frames Cell Events: 7741 mean_circ: 0.944005
Ran for 125550 frames Cell Events: 7979 mean_circ: 0.943963
Ran for 126550 frames Cell Events: 8045 mean_circ: 0.943946
Ran for 127550 frames Cell Events: 8099 mean_circ: 0.943976



End this measurment..



Stopping time: 2.95219e+008
****************************************
Measurement finished

Mean Circularitiy: 0.944017
Mean Area: 594.222
Mean FL-1 intensity: 3451.3
Mean FL-2 intensity: 5377.77
Mean FL-3 intensity: 0

frames processed: 127154
Total Time: 64019.4ms
Number of Runs (new bg img) 0
Frames collected: 127870
Skipped frames: 71184
Rescued Frames: 4012249
Samples DAQ: 31968563
Samples written: 63934988
Samples per Channel: 31967494
FPS: 1997.36

Samples per second: 499341
Expected samples/s: 500000

Events: 8119
Events per second: 126.821
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :202924
# 1 frames :41840
# 2 frames :2467
# 3 frames :806
# 4 frames :503
# 5 frames :200
# 6 frames :1448
# 7 frames :1056
# 8 frames :837
# 9 frames :547
# 10 frames :686
# 11 frames :372
# 12 frames :266
# 13 frames :155
# 14 frames :163
# 15 frames :236
# 16 frames :181
# 17 frames :159
# 18 frames :126
# 19 frames :92
# 20 frames :54
# 21 frames :66
# 22 frames :60
# 23 frames :50
# 24 frames :44
# 25 frames :46
# 26 frames :30
# 27 frames :31
# 28 frames :26
# 29 frames :31
# 30 frames :13
# 31 frames :19
# 32 frames :18
# 33 frames :19
# 34 frames :5
# 35 frames :13
# 36 frames :6
# 37 frames :2
# 38 frames :7
# 39 frames :2
# 40 frames :8
# 41 frames :5
# 42 frames :6
# 43 frames :4
# 44 frames :3
# 45 frames :7
# 46 frames :2
# 47 frames :1
# 48 frames :2
# 49 frames :3
# 50 frames :1
# 51 frames :2
# 52 frames :3
# 55 frames :1
# 56 frames :1
# 59 frames :1
# 61 frames :1
# 68 frames :1
# 70 frames :1
# 89 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.398
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0