Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.11.14 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c0e0ab365f1d9d4718507dcbbf313c2c",
"name": "M1_20170518_1910_dsRNA_J3_fast.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:11:41"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8106 (max 8107) in '8106'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.5065
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.98064e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 47 mean_circ: 0.94189
Ran for 1550 frames Cell Events: 70 mean_circ: 0.938109
Ran for 2050 frames Cell Events: 95 mean_circ: 0.940921
Ran for 2550 frames Cell Events: 128 mean_circ: 0.939377
Ran for 3550 frames Cell Events: 174 mean_circ: 0.939311
Ran for 4050 frames Cell Events: 183 mean_circ: 0.938858
Ran for 4550 frames Cell Events: 221 mean_circ: 0.937628
Ran for 5050 frames Cell Events: 244 mean_circ: 0.938117
Ran for 6550 frames Cell Events: 303 mean_circ: 0.940243
Ran for 7550 frames Cell Events: 350 mean_circ: 0.940413
Ran for 9050 frames Cell Events: 421 mean_circ: 0.940672
Ran for 9550 frames Cell Events: 441 mean_circ: 0.940528
Ran for 10050 frames Cell Events: 460 mean_circ: 0.940907
Ran for 11050 frames Cell Events: 521 mean_circ: 0.940913
Ran for 12550 frames Cell Events: 591 mean_circ: 0.941541
Ran for 13050 frames Cell Events: 608 mean_circ: 0.941243
Ran for 13550 frames Cell Events: 640 mean_circ: 0.941077
Ran for 14550 frames Cell Events: 688 mean_circ: 0.94086
Ran for 16050 frames Cell Events: 750 mean_circ: 0.941245
Ran for 16550 frames Cell Events: 768 mean_circ: 0.941234
Ran for 17550 frames Cell Events: 819 mean_circ: 0.940932
Ran for 18050 frames Cell Events: 842 mean_circ: 0.94058
Ran for 18550 frames Cell Events: 865 mean_circ: 0.940663
Ran for 19050 frames Cell Events: 898 mean_circ: 0.941273
Ran for 19550 frames Cell Events: 923 mean_circ: 0.941121
Ran for 20050 frames Cell Events: 947 mean_circ: 0.941348
Ran for 20550 frames Cell Events: 972 mean_circ: 0.941816
Ran for 21550 frames Cell Events: 1017 mean_circ: 0.941357
Ran for 22050 frames Cell Events: 1047 mean_circ: 0.941143
Ran for 22550 frames Cell Events: 1067 mean_circ: 0.941327
Ran for 23050 frames Cell Events: 1094 mean_circ: 0.941164
Ran for 23550 frames Cell Events: 1122 mean_circ: 0.941132
Ran for 24050 frames Cell Events: 1165 mean_circ: 0.941132
Ran for 24550 frames Cell Events: 1188 mean_circ: 0.941311
Ran for 25050 frames Cell Events: 1218 mean_circ: 0.941026
Ran for 26050 frames Cell Events: 1283 mean_circ: 0.940957
Ran for 27050 frames Cell Events: 1356 mean_circ: 0.940398
Ran for 27550 frames Cell Events: 1376 mean_circ: 0.940479
Ran for 29050 frames Cell Events: 1441 mean_circ: 0.940395
Ran for 29550 frames Cell Events: 1478 mean_circ: 0.940376
Ran for 31050 frames Cell Events: 1580 mean_circ: 0.940045
Ran for 33050 frames Cell Events: 1693 mean_circ: 0.94014
Ran for 33550 frames Cell Events: 1725 mean_circ: 0.940141
Ran for 34050 frames Cell Events: 1750 mean_circ: 0.940225
Ran for 34550 frames Cell Events: 1776 mean_circ: 0.940362
Ran for 35550 frames Cell Events: 1813 mean_circ: 0.940491
Ran for 36050 frames Cell Events: 1849 mean_circ: 0.940755
Ran for 36550 frames Cell Events: 1888 mean_circ: 0.940718
Ran for 37050 frames Cell Events: 1914 mean_circ: 0.94073
Ran for 38050 frames Cell Events: 1969 mean_circ: 0.940697
Ran for 38550 frames Cell Events: 1996 mean_circ: 0.940596
Ran for 40050 frames Cell Events: 2072 mean_circ: 0.940716
Ran for 41550 frames Cell Events: 2148 mean_circ: 0.940604
Ran for 42050 frames Cell Events: 2175 mean_circ: 0.940652
Ran for 44050 frames Cell Events: 2284 mean_circ: 0.940693
Ran for 44550 frames Cell Events: 2305 mean_circ: 0.940565
Ran for 45050 frames Cell Events: 2326 mean_circ: 0.940304
Ran for 46550 frames Cell Events: 2403 mean_circ: 0.940069
Ran for 47050 frames Cell Events: 2427 mean_circ: 0.939811
Ran for 49050 frames Cell Events: 2529 mean_circ: 0.939597
Ran for 50550 frames Cell Events: 2605 mean_circ: 0.939303
Ran for 51050 frames Cell Events: 2626 mean_circ: 0.939231
Ran for 51550 frames Cell Events: 2643 mean_circ: 0.939256
Ran for 53550 frames Cell Events: 2754 mean_circ: 0.939314
Ran for 54050 frames Cell Events: 2777 mean_circ: 0.939327
Ran for 54550 frames Cell Events: 2800 mean_circ: 0.939338
Ran for 56050 frames Cell Events: 2883 mean_circ: 0.938996
Ran for 56550 frames Cell Events: 2904 mean_circ: 0.93894
Ran for 57550 frames Cell Events: 2949 mean_circ: 0.938978
Ran for 58050 frames Cell Events: 2973 mean_circ: 0.938976
Ran for 59050 frames Cell Events: 3029 mean_circ: 0.938877
Ran for 59550 frames Cell Events: 3059 mean_circ: 0.938899
Ran for 60050 frames Cell Events: 3077 mean_circ: 0.938919
Ran for 61050 frames Cell Events: 3137 mean_circ: 0.939029
Ran for 63050 frames Cell Events: 3238 mean_circ: 0.938827
Ran for 63550 frames Cell Events: 3261 mean_circ: 0.938874
Ran for 64550 frames Cell Events: 3331 mean_circ: 0.938868
Ran for 65550 frames Cell Events: 3388 mean_circ: 0.938971
Ran for 66050 frames Cell Events: 3416 mean_circ: 0.939042
Ran for 66550 frames Cell Events: 3445 mean_circ: 0.939092
Ran for 67050 frames Cell Events: 3482 mean_circ: 0.939006
Ran for 67550 frames Cell Events: 3498 mean_circ: 0.93903
Ran for 68550 frames Cell Events: 3555 mean_circ: 0.939024
Ran for 69050 frames Cell Events: 3581 mean_circ: 0.939045
Ran for 69550 frames Cell Events: 3603 mean_circ: 0.939055
Ran for 70050 frames Cell Events: 3629 mean_circ: 0.939048
Ran for 70550 frames Cell Events: 3654 mean_circ: 0.938928
Ran for 72050 frames Cell Events: 3740 mean_circ: 0.93884
Ran for 72550 frames Cell Events: 3755 mean_circ: 0.938903
Ran for 74550 frames Cell Events: 3873 mean_circ: 0.93867
Ran for 75550 frames Cell Events: 3926 mean_circ: 0.938651
Ran for 76550 frames Cell Events: 3974 mean_circ: 0.93859
Ran for 79050 frames Cell Events: 4106 mean_circ: 0.938446
Ran for 79550 frames Cell Events: 4140 mean_circ: 0.938455
Ran for 81050 frames Cell Events: 4225 mean_circ: 0.93845
Ran for 81550 frames Cell Events: 4247 mean_circ: 0.938459
Ran for 84550 frames Cell Events: 4403 mean_circ: 0.938674
Ran for 85050 frames Cell Events: 4429 mean_circ: 0.938698
Ran for 86050 frames Cell Events: 4486 mean_circ: 0.938598
Ran for 86550 frames Cell Events: 4511 mean_circ: 0.93863
Ran for 87050 frames Cell Events: 4545 mean_circ: 0.9386
Ran for 88050 frames Cell Events: 4612 mean_circ: 0.938623
Ran for 88550 frames Cell Events: 4633 mean_circ: 0.938665
Ran for 92050 frames Cell Events: 4807 mean_circ: 0.938615
Ran for 94050 frames Cell Events: 4918 mean_circ: 0.938618
Ran for 94550 frames Cell Events: 4938 mean_circ: 0.938667
Ran for 95050 frames Cell Events: 4966 mean_circ: 0.938671
Ran for 96050 frames Cell Events: 5012 mean_circ: 0.938743
Ran for 97050 frames Cell Events: 5069 mean_circ: 0.938684
Ran for 97550 frames Cell Events: 5093 mean_circ: 0.938691
Ran for 98050 frames Cell Events: 5122 mean_circ: 0.938766
Ran for 98550 frames Cell Events: 5156 mean_circ: 0.938813
Ran for 99050 frames Cell Events: 5194 mean_circ: 0.938808
Ran for 99550 frames Cell Events: 5217 mean_circ: 0.938876
Ran for 100050 frames Cell Events: 5250 mean_circ: 0.938799
Ran for 101550 frames Cell Events: 5343 mean_circ: 0.938697
Ran for 102050 frames Cell Events: 5375 mean_circ: 0.938697
Ran for 103550 frames Cell Events: 5455 mean_circ: 0.938753
Ran for 104550 frames Cell Events: 5509 mean_circ: 0.938773
Ran for 105050 frames Cell Events: 5540 mean_circ: 0.938718
Ran for 105550 frames Cell Events: 5567 mean_circ: 0.938779
Ran for 106550 frames Cell Events: 5613 mean_circ: 0.938797
Ran for 108050 frames Cell Events: 5698 mean_circ: 0.938605
Ran for 109050 frames Cell Events: 5742 mean_circ: 0.938622
Ran for 110050 frames Cell Events: 5791 mean_circ: 0.938647
Ran for 110550 frames Cell Events: 5820 mean_circ: 0.938678
Ran for 111050 frames Cell Events: 5843 mean_circ: 0.938686
Ran for 111550 frames Cell Events: 5868 mean_circ: 0.938596
Ran for 112550 frames Cell Events: 5929 mean_circ: 0.938558
Ran for 113050 frames Cell Events: 5959 mean_circ: 0.938538
Ran for 113550 frames Cell Events: 5977 mean_circ: 0.938496
Ran for 114550 frames Cell Events: 6028 mean_circ: 0.938505
Ran for 115050 frames Cell Events: 6061 mean_circ: 0.938507
Ran for 115550 frames Cell Events: 6080 mean_circ: 0.938497
Ran for 116050 frames Cell Events: 6090 mean_circ: 0.938528
Ran for 117050 frames Cell Events: 6132 mean_circ: 0.938557
Ran for 118050 frames Cell Events: 6177 mean_circ: 0.938648
Ran for 121050 frames Cell Events: 6372 mean_circ: 0.938683
Ran for 122550 frames Cell Events: 6456 mean_circ: 0.938552
Ran for 124050 frames Cell Events: 6546 mean_circ: 0.938621
Ran for 124550 frames Cell Events: 6572 mean_circ: 0.938646
Ran for 125550 frames Cell Events: 6641 mean_circ: 0.938649
Ran for 126050 frames Cell Events: 6668 mean_circ: 0.938675
Ran for 127550 frames Cell Events: 6731 mean_circ: 0.938583
Ran for 128050 frames Cell Events: 6759 mean_circ: 0.93854
Ran for 128550 frames Cell Events: 6777 mean_circ: 0.938566
Ran for 130550 frames Cell Events: 6884 mean_circ: 0.938523
Ran for 132050 frames Cell Events: 6955 mean_circ: 0.93849
Ran for 132550 frames Cell Events: 6977 mean_circ: 0.938528
Ran for 134050 frames Cell Events: 7036 mean_circ: 0.938488
Ran for 134550 frames Cell Events: 7063 mean_circ: 0.938514
Ran for 135550 frames Cell Events: 7116 mean_circ: 0.938555
Ran for 136050 frames Cell Events: 7144 mean_circ: 0.938562
Ran for 138050 frames Cell Events: 7263 mean_circ: 0.938571
Ran for 139050 frames Cell Events: 7308 mean_circ: 0.938573
Ran for 140050 frames Cell Events: 7363 mean_circ: 0.938598
Ran for 141550 frames Cell Events: 7441 mean_circ: 0.938501
Ran for 142050 frames Cell Events: 7465 mean_circ: 0.938533
Ran for 143550 frames Cell Events: 7549 mean_circ: 0.938566
Ran for 144050 frames Cell Events: 7572 mean_circ: 0.938593
Ran for 144550 frames Cell Events: 7597 mean_circ: 0.938611
Ran for 145050 frames Cell Events: 7621 mean_circ: 0.938598
Ran for 145550 frames Cell Events: 7658 mean_circ: 0.938598
Ran for 146550 frames Cell Events: 7724 mean_circ: 0.93856
Ran for 147050 frames Cell Events: 7751 mean_circ: 0.938551
Ran for 149050 frames Cell Events: 7865 mean_circ: 0.938566
Ran for 150050 frames Cell Events: 7929 mean_circ: 0.938555
Ran for 151050 frames Cell Events: 7977 mean_circ: 0.938558
Ran for 151550 frames Cell Events: 7999 mean_circ: 0.938573
Ran for 152550 frames Cell Events: 8066 mean_circ: 0.938513
Ran for 153050 frames Cell Events: 8084 mean_circ: 0.938532



End this measurment..



Stopping time: 2.98141e+008
****************************************
Measurement finished

Mean Circularitiy: 0.938514
Mean Area: 622.222
Mean FL-1 intensity: 3060.08
Mean FL-2 intensity: 5416.11
Mean FL-3 intensity: 0

frames processed: 152958
Total Time: 76782.9ms
Number of Runs (new bg img) 0
Frames collected: 153399
Skipped frames: 82229
Rescued Frames: 4650813
Samples DAQ: 38351216
Samples written: 76699246
Samples per Channel: 38349623
FPS: 1997.83

Samples per second: 499455
Expected samples/s: 500000

Events: 8107
Events per second: 105.583
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :233833
# 1 frames :59998
# 2 frames :3114
# 3 frames :1056
# 4 frames :652
# 5 frames :260
# 6 frames :1321
# 7 frames :1504
# 8 frames :968
# 9 frames :703
# 10 frames :846
# 11 frames :454
# 12 frames :306
# 13 frames :191
# 14 frames :169
# 15 frames :247
# 16 frames :181
# 17 frames :172
# 18 frames :114
# 19 frames :90
# 20 frames :54
# 21 frames :60
# 22 frames :54
# 23 frames :42
# 24 frames :41
# 25 frames :27
# 26 frames :37
# 27 frames :23
# 28 frames :23
# 29 frames :13
# 30 frames :12
# 31 frames :14
# 32 frames :9
# 33 frames :7
# 34 frames :5
# 35 frames :9
# 36 frames :4
# 37 frames :4
# 38 frames :6
# 39 frames :5
# 40 frames :4
# 41 frames :2
# 43 frames :3
# 44 frames :2
# 45 frames :2
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :2
# 56 frames :1
# 57 frames :1
# 64 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.5065
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0