Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.11.54 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "417a27853d770515b7f473eba6b6f7a1",
"name": "M1_20170518_2001_dsRNA_J4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:12:22"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8106 (max 8107) in '8106'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.413
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.01133e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 68 mean_circ: 0.952193
Ran for 1550 frames Cell Events: 93 mean_circ: 0.948879
Ran for 2550 frames Cell Events: 153 mean_circ: 0.944804
Ran for 4050 frames Cell Events: 261 mean_circ: 0.944992
Ran for 4550 frames Cell Events: 282 mean_circ: 0.944246
Ran for 5050 frames Cell Events: 309 mean_circ: 0.943895
Ran for 6050 frames Cell Events: 370 mean_circ: 0.942824
Ran for 6550 frames Cell Events: 402 mean_circ: 0.943346
Ran for 7050 frames Cell Events: 419 mean_circ: 0.943443
Ran for 9550 frames Cell Events: 540 mean_circ: 0.943066
Ran for 10050 frames Cell Events: 555 mean_circ: 0.942951
Ran for 10550 frames Cell Events: 582 mean_circ: 0.94282
Ran for 11050 frames Cell Events: 604 mean_circ: 0.942319
Ran for 13050 frames Cell Events: 729 mean_circ: 0.942567
Ran for 13550 frames Cell Events: 748 mean_circ: 0.942961
Ran for 14050 frames Cell Events: 776 mean_circ: 0.943583
Ran for 14550 frames Cell Events: 792 mean_circ: 0.943914
Ran for 15050 frames Cell Events: 823 mean_circ: 0.943884
Ran for 16050 frames Cell Events: 879 mean_circ: 0.944661
Ran for 16550 frames Cell Events: 918 mean_circ: 0.944496
Ran for 17550 frames Cell Events: 963 mean_circ: 0.944447
Ran for 18050 frames Cell Events: 987 mean_circ: 0.944569
Ran for 18550 frames Cell Events: 1003 mean_circ: 0.94423
Ran for 20550 frames Cell Events: 1087 mean_circ: 0.943883
Ran for 22550 frames Cell Events: 1205 mean_circ: 0.944377
Ran for 23050 frames Cell Events: 1241 mean_circ: 0.943955
Ran for 26550 frames Cell Events: 1434 mean_circ: 0.94381
Ran for 27050 frames Cell Events: 1457 mean_circ: 0.943825
Ran for 27550 frames Cell Events: 1481 mean_circ: 0.943976
Ran for 28050 frames Cell Events: 1506 mean_circ: 0.944051
Ran for 28550 frames Cell Events: 1536 mean_circ: 0.944014
Ran for 30050 frames Cell Events: 1603 mean_circ: 0.943981
Ran for 30550 frames Cell Events: 1622 mean_circ: 0.943945
Ran for 31050 frames Cell Events: 1649 mean_circ: 0.944046
Ran for 31550 frames Cell Events: 1677 mean_circ: 0.943751
Ran for 32550 frames Cell Events: 1722 mean_circ: 0.943911
Ran for 33050 frames Cell Events: 1749 mean_circ: 0.943832
Ran for 33550 frames Cell Events: 1774 mean_circ: 0.944018
Ran for 36050 frames Cell Events: 1923 mean_circ: 0.943988
Ran for 37050 frames Cell Events: 1974 mean_circ: 0.943738
Ran for 37550 frames Cell Events: 1995 mean_circ: 0.943837
Ran for 38050 frames Cell Events: 2022 mean_circ: 0.943913
Ran for 38550 frames Cell Events: 2048 mean_circ: 0.94384
Ran for 39050 frames Cell Events: 2069 mean_circ: 0.94402
Ran for 39550 frames Cell Events: 2095 mean_circ: 0.943977
Ran for 40050 frames Cell Events: 2125 mean_circ: 0.943842
Ran for 40550 frames Cell Events: 2147 mean_circ: 0.943828
Ran for 41050 frames Cell Events: 2169 mean_circ: 0.94386
Ran for 41550 frames Cell Events: 2195 mean_circ: 0.943969
Ran for 42050 frames Cell Events: 2212 mean_circ: 0.94401
Ran for 43550 frames Cell Events: 2311 mean_circ: 0.944089
Ran for 44050 frames Cell Events: 2325 mean_circ: 0.944037
Ran for 44550 frames Cell Events: 2339 mean_circ: 0.944119
Ran for 45050 frames Cell Events: 2368 mean_circ: 0.944063
Ran for 45550 frames Cell Events: 2408 mean_circ: 0.943959
Ran for 46550 frames Cell Events: 2466 mean_circ: 0.944032
Ran for 47050 frames Cell Events: 2491 mean_circ: 0.944093
Ran for 48050 frames Cell Events: 2531 mean_circ: 0.944165
Ran for 48550 frames Cell Events: 2551 mean_circ: 0.944292
Ran for 49550 frames Cell Events: 2625 mean_circ: 0.944567
Ran for 50050 frames Cell Events: 2660 mean_circ: 0.944597
Ran for 50550 frames Cell Events: 2695 mean_circ: 0.94476
Ran for 51050 frames Cell Events: 2736 mean_circ: 0.944643
Ran for 52550 frames Cell Events: 2857 mean_circ: 0.944448
Ran for 53050 frames Cell Events: 2881 mean_circ: 0.944489
Ran for 57050 frames Cell Events: 3208 mean_circ: 0.944349
Ran for 59550 frames Cell Events: 3334 mean_circ: 0.944377
Ran for 60050 frames Cell Events: 3355 mean_circ: 0.94436
Ran for 60550 frames Cell Events: 3383 mean_circ: 0.944335
Ran for 61050 frames Cell Events: 3408 mean_circ: 0.944347
Ran for 62050 frames Cell Events: 3471 mean_circ: 0.944361
Ran for 62550 frames Cell Events: 3508 mean_circ: 0.94439
Ran for 63050 frames Cell Events: 3531 mean_circ: 0.944249
Ran for 64550 frames Cell Events: 3609 mean_circ: 0.944279
Ran for 67050 frames Cell Events: 3735 mean_circ: 0.944186
Ran for 68550 frames Cell Events: 3819 mean_circ: 0.944201
Ran for 69550 frames Cell Events: 3876 mean_circ: 0.944159
Ran for 70050 frames Cell Events: 3899 mean_circ: 0.944132
Ran for 70550 frames Cell Events: 3936 mean_circ: 0.944249
Ran for 72050 frames Cell Events: 3999 mean_circ: 0.944303
Ran for 73050 frames Cell Events: 4057 mean_circ: 0.944242
Ran for 73550 frames Cell Events: 4093 mean_circ: 0.944292
Ran for 74050 frames Cell Events: 4127 mean_circ: 0.944285
Ran for 76050 frames Cell Events: 4225 mean_circ: 0.94411
Ran for 77050 frames Cell Events: 4280 mean_circ: 0.944248
Ran for 77550 frames Cell Events: 4303 mean_circ: 0.944124
Ran for 78050 frames Cell Events: 4324 mean_circ: 0.943992
Ran for 78550 frames Cell Events: 4358 mean_circ: 0.943828
Ran for 79050 frames Cell Events: 4384 mean_circ: 0.943855
Ran for 79550 frames Cell Events: 4412 mean_circ: 0.943872
Ran for 80550 frames Cell Events: 4454 mean_circ: 0.943729
Ran for 81050 frames Cell Events: 4472 mean_circ: 0.943725
Ran for 81550 frames Cell Events: 4490 mean_circ: 0.943725
Ran for 82050 frames Cell Events: 4523 mean_circ: 0.943677
Ran for 83050 frames Cell Events: 4576 mean_circ: 0.943747
Ran for 83550 frames Cell Events: 4599 mean_circ: 0.943714
Ran for 84050 frames Cell Events: 4618 mean_circ: 0.943722
Ran for 84550 frames Cell Events: 4642 mean_circ: 0.943685
Ran for 86050 frames Cell Events: 4707 mean_circ: 0.943665
Ran for 86550 frames Cell Events: 4739 mean_circ: 0.943723
Ran for 87050 frames Cell Events: 4776 mean_circ: 0.943723
Ran for 89550 frames Cell Events: 4941 mean_circ: 0.943837
Ran for 90050 frames Cell Events: 4975 mean_circ: 0.943833
Ran for 91050 frames Cell Events: 5038 mean_circ: 0.94388
Ran for 91550 frames Cell Events: 5065 mean_circ: 0.943808
Ran for 92050 frames Cell Events: 5083 mean_circ: 0.943738
Ran for 92550 frames Cell Events: 5109 mean_circ: 0.943755
Ran for 93050 frames Cell Events: 5127 mean_circ: 0.943677
Ran for 93550 frames Cell Events: 5156 mean_circ: 0.943653
Ran for 94050 frames Cell Events: 5193 mean_circ: 0.943659
Ran for 95050 frames Cell Events: 5267 mean_circ: 0.94383
Ran for 98550 frames Cell Events: 5456 mean_circ: 0.943948
Ran for 99050 frames Cell Events: 5488 mean_circ: 0.943961
Ran for 99550 frames Cell Events: 5510 mean_circ: 0.943981
Ran for 103550 frames Cell Events: 5716 mean_circ: 0.943911
Ran for 104050 frames Cell Events: 5732 mean_circ: 0.943982
Ran for 104550 frames Cell Events: 5760 mean_circ: 0.944022
Ran for 105050 frames Cell Events: 5769 mean_circ: 0.94402
Ran for 105550 frames Cell Events: 5799 mean_circ: 0.943988
Ran for 106550 frames Cell Events: 5847 mean_circ: 0.943955
Ran for 107550 frames Cell Events: 5905 mean_circ: 0.943868
Ran for 109050 frames Cell Events: 5997 mean_circ: 0.943887
Ran for 110050 frames Cell Events: 6056 mean_circ: 0.943892
Ran for 110550 frames Cell Events: 6079 mean_circ: 0.94387
Ran for 111050 frames Cell Events: 6104 mean_circ: 0.943867
Ran for 111550 frames Cell Events: 6124 mean_circ: 0.943931
Ran for 112550 frames Cell Events: 6166 mean_circ: 0.943848
Ran for 114550 frames Cell Events: 6319 mean_circ: 0.943721
Ran for 115050 frames Cell Events: 6350 mean_circ: 0.943696
Ran for 115550 frames Cell Events: 6370 mean_circ: 0.94368
Ran for 116050 frames Cell Events: 6407 mean_circ: 0.943641
Ran for 117550 frames Cell Events: 6508 mean_circ: 0.943601
Ran for 118050 frames Cell Events: 6531 mean_circ: 0.943571
Ran for 118550 frames Cell Events: 6555 mean_circ: 0.94357
Ran for 119550 frames Cell Events: 6614 mean_circ: 0.943539
Ran for 120550 frames Cell Events: 6667 mean_circ: 0.943576
Ran for 121550 frames Cell Events: 6730 mean_circ: 0.943623
Ran for 122550 frames Cell Events: 6796 mean_circ: 0.943644
Ran for 124050 frames Cell Events: 6847 mean_circ: 0.943743
Ran for 125050 frames Cell Events: 6918 mean_circ: 0.943792
Ran for 126050 frames Cell Events: 7000 mean_circ: 0.943757
Ran for 126550 frames Cell Events: 7022 mean_circ: 0.94372
Ran for 130050 frames Cell Events: 7235 mean_circ: 0.943753
Ran for 130550 frames Cell Events: 7259 mean_circ: 0.943769
Ran for 131050 frames Cell Events: 7278 mean_circ: 0.94375
Ran for 132050 frames Cell Events: 7338 mean_circ: 0.943699
Ran for 133550 frames Cell Events: 7423 mean_circ: 0.94366
Ran for 134050 frames Cell Events: 7458 mean_circ: 0.943599
Ran for 135050 frames Cell Events: 7520 mean_circ: 0.943512
Ran for 135550 frames Cell Events: 7543 mean_circ: 0.943526
Ran for 136550 frames Cell Events: 7583 mean_circ: 0.943573
Ran for 138050 frames Cell Events: 7655 mean_circ: 0.943567
Ran for 138550 frames Cell Events: 7684 mean_circ: 0.943538
Ran for 139050 frames Cell Events: 7709 mean_circ: 0.943519
Ran for 140050 frames Cell Events: 7777 mean_circ: 0.943429
Ran for 140550 frames Cell Events: 7798 mean_circ: 0.943435
Ran for 144050 frames Cell Events: 8023 mean_circ: 0.943336
Ran for 144550 frames Cell Events: 8041 mean_circ: 0.943278



End this measurment..



Stopping time: 3.01206e+008
****************************************
Measurement finished

Mean Circularitiy: 0.943187
Mean Area: 672.935
Mean FL-1 intensity: 3448.14
Mean FL-2 intensity: 5088.08
Mean FL-3 intensity: 0

frames processed: 143820
Total Time: 72972.5ms
Number of Runs (new bg img) 0
Frames collected: 145772
Skipped frames: 84181
Rescued Frames: 4720667
Samples DAQ: 36443404
Samples written: 72885742
Samples per Channel: 36442871
FPS: 1997.63

Samples per second: 499406
Expected samples/s: 500000

Events: 8108
Events per second: 111.11
jumped back (PMT): 3631
#Frames read, frequency
# 0 frames :217365
# 1 frames :62304
# 2 frames :3088
# 3 frames :816
# 4 frames :424
# 5 frames :156
# 6 frames :1970
# 7 frames :1487
# 8 frames :955
# 9 frames :501
# 10 frames :459
# 11 frames :197
# 12 frames :207
# 13 frames :139
# 14 frames :218
# 15 frames :214
# 16 frames :138
# 17 frames :89
# 18 frames :84
# 19 frames :38
# 20 frames :56
# 21 frames :78
# 22 frames :50
# 23 frames :43
# 24 frames :35
# 25 frames :26
# 26 frames :19
# 27 frames :26
# 28 frames :15
# 29 frames :14
# 30 frames :12
# 31 frames :17
# 32 frames :10
# 33 frames :7
# 34 frames :9
# 35 frames :4
# 36 frames :11
# 37 frames :2
# 38 frames :3
# 39 frames :8
# 40 frames :3
# 41 frames :4
# 42 frames :4
# 43 frames :8
# 44 frames :4
# 45 frames :1
# 46 frames :4
# 47 frames :3
# 48 frames :3
# 49 frames :2
# 50 frames :1
# 51 frames :3
# 52 frames :2
# 53 frames :1
# 56 frames :1
# 57 frames :2
# 59 frames :2
# 63 frames :1
# 69 frames :1
# 72 frames :1
# 73 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.413
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0