Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.13.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2980e1ac568b52e889662aafa199a71d",
"name": "M1_20170518_2016_dsRNA_J5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:13:57"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8007 (max 8008) in '8007'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.4664
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.02044e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 28 mean_circ: 0.949065
Ran for 1550 frames Cell Events: 80 mean_circ: 0.929031
Ran for 2050 frames Cell Events: 95 mean_circ: 0.935005
Ran for 4050 frames Cell Events: 195 mean_circ: 0.93738
Ran for 6550 frames Cell Events: 302 mean_circ: 0.937119
Ran for 7050 frames Cell Events: 332 mean_circ: 0.938266
Ran for 7550 frames Cell Events: 355 mean_circ: 0.938038
Ran for 9050 frames Cell Events: 427 mean_circ: 0.938985
Ran for 9550 frames Cell Events: 453 mean_circ: 0.939486
Ran for 10050 frames Cell Events: 476 mean_circ: 0.939335
Ran for 11050 frames Cell Events: 522 mean_circ: 0.939475
Ran for 11550 frames Cell Events: 561 mean_circ: 0.939035
Ran for 12050 frames Cell Events: 582 mean_circ: 0.93938
Ran for 12550 frames Cell Events: 611 mean_circ: 0.939385
Ran for 14050 frames Cell Events: 699 mean_circ: 0.939583
Ran for 14550 frames Cell Events: 725 mean_circ: 0.939679
Ran for 15550 frames Cell Events: 766 mean_circ: 0.939517
Ran for 16050 frames Cell Events: 789 mean_circ: 0.93966
Ran for 17550 frames Cell Events: 872 mean_circ: 0.939234
Ran for 18050 frames Cell Events: 907 mean_circ: 0.939226
Ran for 18550 frames Cell Events: 926 mean_circ: 0.939378
Ran for 19050 frames Cell Events: 953 mean_circ: 0.939391
Ran for 19550 frames Cell Events: 988 mean_circ: 0.93925
Ran for 20050 frames Cell Events: 1025 mean_circ: 0.938746
Ran for 21050 frames Cell Events: 1068 mean_circ: 0.938997
Ran for 21550 frames Cell Events: 1088 mean_circ: 0.938711
Ran for 22550 frames Cell Events: 1147 mean_circ: 0.93858
Ran for 23050 frames Cell Events: 1174 mean_circ: 0.938647
Ran for 24050 frames Cell Events: 1229 mean_circ: 0.938457
Ran for 24550 frames Cell Events: 1259 mean_circ: 0.938507
Ran for 25550 frames Cell Events: 1308 mean_circ: 0.938311
Ran for 29550 frames Cell Events: 1569 mean_circ: 0.939109
Ran for 30050 frames Cell Events: 1601 mean_circ: 0.939177
Ran for 30550 frames Cell Events: 1638 mean_circ: 0.939223
Ran for 31050 frames Cell Events: 1670 mean_circ: 0.939208
Ran for 31550 frames Cell Events: 1696 mean_circ: 0.939266
Ran for 32550 frames Cell Events: 1740 mean_circ: 0.939255
Ran for 34550 frames Cell Events: 1854 mean_circ: 0.93906
Ran for 35050 frames Cell Events: 1874 mean_circ: 0.938886
Ran for 36050 frames Cell Events: 1912 mean_circ: 0.939093
Ran for 36550 frames Cell Events: 1935 mean_circ: 0.939082
Ran for 38050 frames Cell Events: 2003 mean_circ: 0.938731
Ran for 38550 frames Cell Events: 2037 mean_circ: 0.938748
Ran for 39550 frames Cell Events: 2103 mean_circ: 0.938635
Ran for 40050 frames Cell Events: 2139 mean_circ: 0.938611
Ran for 41050 frames Cell Events: 2204 mean_circ: 0.938271
Ran for 41550 frames Cell Events: 2232 mean_circ: 0.93813
Ran for 42050 frames Cell Events: 2262 mean_circ: 0.938134
Ran for 44050 frames Cell Events: 2369 mean_circ: 0.93822
Ran for 45550 frames Cell Events: 2444 mean_circ: 0.938073
Ran for 46050 frames Cell Events: 2474 mean_circ: 0.938168
Ran for 46550 frames Cell Events: 2498 mean_circ: 0.938024
Ran for 47050 frames Cell Events: 2519 mean_circ: 0.938031
Ran for 47550 frames Cell Events: 2562 mean_circ: 0.938056
Ran for 48550 frames Cell Events: 2611 mean_circ: 0.938168
Ran for 50050 frames Cell Events: 2656 mean_circ: 0.938153
Ran for 53550 frames Cell Events: 2867 mean_circ: 0.938185
Ran for 54550 frames Cell Events: 2904 mean_circ: 0.938237
Ran for 55050 frames Cell Events: 2931 mean_circ: 0.938259
Ran for 55550 frames Cell Events: 2960 mean_circ: 0.93829
Ran for 60050 frames Cell Events: 3179 mean_circ: 0.938201
Ran for 60550 frames Cell Events: 3206 mean_circ: 0.938187
Ran for 61050 frames Cell Events: 3226 mean_circ: 0.93814
Ran for 61550 frames Cell Events: 3254 mean_circ: 0.937957
Ran for 62050 frames Cell Events: 3271 mean_circ: 0.937962
Ran for 62550 frames Cell Events: 3291 mean_circ: 0.937827
Ran for 63050 frames Cell Events: 3315 mean_circ: 0.937733
Ran for 63550 frames Cell Events: 3337 mean_circ: 0.937729
Ran for 65050 frames Cell Events: 3417 mean_circ: 0.93765
Ran for 66550 frames Cell Events: 3512 mean_circ: 0.937633
Ran for 67050 frames Cell Events: 3537 mean_circ: 0.937592
Ran for 67550 frames Cell Events: 3560 mean_circ: 0.937683
Ran for 68050 frames Cell Events: 3581 mean_circ: 0.93762
Ran for 68550 frames Cell Events: 3611 mean_circ: 0.93767
Ran for 69050 frames Cell Events: 3638 mean_circ: 0.937742
Ran for 69550 frames Cell Events: 3660 mean_circ: 0.937688
Ran for 71050 frames Cell Events: 3741 mean_circ: 0.937562
Ran for 71550 frames Cell Events: 3771 mean_circ: 0.937572
Ran for 73050 frames Cell Events: 3858 mean_circ: 0.937409
Ran for 73550 frames Cell Events: 3886 mean_circ: 0.937292
Ran for 74550 frames Cell Events: 3949 mean_circ: 0.937108
Ran for 75050 frames Cell Events: 3977 mean_circ: 0.937149
Ran for 76050 frames Cell Events: 4019 mean_circ: 0.937233
Ran for 76550 frames Cell Events: 4039 mean_circ: 0.937262
Ran for 77050 frames Cell Events: 4062 mean_circ: 0.937177
Ran for 78050 frames Cell Events: 4097 mean_circ: 0.937246
Ran for 78550 frames Cell Events: 4118 mean_circ: 0.937291
Ran for 79050 frames Cell Events: 4136 mean_circ: 0.937298
Ran for 80550 frames Cell Events: 4214 mean_circ: 0.937315
Ran for 81550 frames Cell Events: 4270 mean_circ: 0.937266
Ran for 82050 frames Cell Events: 4300 mean_circ: 0.937234
Ran for 82550 frames Cell Events: 4323 mean_circ: 0.937144
Ran for 83550 frames Cell Events: 4388 mean_circ: 0.93712
Ran for 84050 frames Cell Events: 4411 mean_circ: 0.937158
Ran for 84550 frames Cell Events: 4434 mean_circ: 0.937156
Ran for 85550 frames Cell Events: 4496 mean_circ: 0.937068
Ran for 86050 frames Cell Events: 4518 mean_circ: 0.937109
Ran for 87550 frames Cell Events: 4597 mean_circ: 0.936888
Ran for 88050 frames Cell Events: 4617 mean_circ: 0.936916
Ran for 90050 frames Cell Events: 4722 mean_circ: 0.936915
Ran for 90550 frames Cell Events: 4742 mean_circ: 0.936881
Ran for 91050 frames Cell Events: 4760 mean_circ: 0.936899
Ran for 91550 frames Cell Events: 4785 mean_circ: 0.936907
Ran for 92050 frames Cell Events: 4808 mean_circ: 0.937049
Ran for 92550 frames Cell Events: 4835 mean_circ: 0.937007
Ran for 93050 frames Cell Events: 4857 mean_circ: 0.936999
Ran for 94050 frames Cell Events: 4931 mean_circ: 0.936967
Ran for 94550 frames Cell Events: 4964 mean_circ: 0.936906
Ran for 96550 frames Cell Events: 5080 mean_circ: 0.93662
Ran for 97050 frames Cell Events: 5106 mean_circ: 0.936686
Ran for 97550 frames Cell Events: 5131 mean_circ: 0.936724
Ran for 98050 frames Cell Events: 5162 mean_circ: 0.936706
Ran for 99050 frames Cell Events: 5217 mean_circ: 0.936545
Ran for 99550 frames Cell Events: 5233 mean_circ: 0.936589
Ran for 100550 frames Cell Events: 5273 mean_circ: 0.936476
Ran for 102550 frames Cell Events: 5389 mean_circ: 0.936439
Ran for 104050 frames Cell Events: 5491 mean_circ: 0.93625
Ran for 104550 frames Cell Events: 5522 mean_circ: 0.936266
Ran for 105050 frames Cell Events: 5548 mean_circ: 0.936273
Ran for 105550 frames Cell Events: 5584 mean_circ: 0.936267
Ran for 106050 frames Cell Events: 5592 mean_circ: 0.936192
Ran for 106550 frames Cell Events: 5618 mean_circ: 0.936177
Ran for 107050 frames Cell Events: 5649 mean_circ: 0.936256
Ran for 107550 frames Cell Events: 5674 mean_circ: 0.936289
Ran for 108050 frames Cell Events: 5703 mean_circ: 0.936376
Ran for 109050 frames Cell Events: 5754 mean_circ: 0.936443
Ran for 109550 frames Cell Events: 5784 mean_circ: 0.936327
Ran for 110050 frames Cell Events: 5813 mean_circ: 0.936289
Ran for 110550 frames Cell Events: 5834 mean_circ: 0.936287
Ran for 112550 frames Cell Events: 5952 mean_circ: 0.936082
Ran for 115050 frames Cell Events: 6095 mean_circ: 0.935943
Ran for 116050 frames Cell Events: 6153 mean_circ: 0.936052
Ran for 116550 frames Cell Events: 6187 mean_circ: 0.93604
Ran for 117050 frames Cell Events: 6221 mean_circ: 0.936033
Ran for 118050 frames Cell Events: 6285 mean_circ: 0.935999
Ran for 118550 frames Cell Events: 6314 mean_circ: 0.93605
Ran for 119050 frames Cell Events: 6338 mean_circ: 0.936032
Ran for 121050 frames Cell Events: 6468 mean_circ: 0.936068
Ran for 122050 frames Cell Events: 6526 mean_circ: 0.936077
Ran for 123050 frames Cell Events: 6577 mean_circ: 0.935978
Ran for 123550 frames Cell Events: 6601 mean_circ: 0.936022
Ran for 124050 frames Cell Events: 6617 mean_circ: 0.93599
Ran for 124550 frames Cell Events: 6644 mean_circ: 0.936037
Ran for 125050 frames Cell Events: 6673 mean_circ: 0.93603
Ran for 125550 frames Cell Events: 6705 mean_circ: 0.935964
Ran for 126050 frames Cell Events: 6729 mean_circ: 0.935995
Ran for 126550 frames Cell Events: 6763 mean_circ: 0.93597
Ran for 127050 frames Cell Events: 6792 mean_circ: 0.93585
Ran for 127550 frames Cell Events: 6822 mean_circ: 0.935876
Ran for 128050 frames Cell Events: 6843 mean_circ: 0.935922
Ran for 128550 frames Cell Events: 6880 mean_circ: 0.935958
Ran for 129050 frames Cell Events: 6909 mean_circ: 0.935961
Ran for 129550 frames Cell Events: 6931 mean_circ: 0.935957
Ran for 130050 frames Cell Events: 6955 mean_circ: 0.935968
Ran for 131550 frames Cell Events: 7007 mean_circ: 0.936025
Ran for 133050 frames Cell Events: 7102 mean_circ: 0.936008
Ran for 133550 frames Cell Events: 7129 mean_circ: 0.936022
Ran for 134050 frames Cell Events: 7155 mean_circ: 0.936018
Ran for 136050 frames Cell Events: 7254 mean_circ: 0.936056
Ran for 136550 frames Cell Events: 7285 mean_circ: 0.93607
Ran for 137050 frames Cell Events: 7327 mean_circ: 0.936045
Ran for 137550 frames Cell Events: 7358 mean_circ: 0.936066
Ran for 138050 frames Cell Events: 7383 mean_circ: 0.936038
Ran for 138550 frames Cell Events: 7415 mean_circ: 0.936023
Ran for 139550 frames Cell Events: 7463 mean_circ: 0.935996
Ran for 141550 frames Cell Events: 7578 mean_circ: 0.935916
Ran for 142550 frames Cell Events: 7631 mean_circ: 0.935911
Ran for 143050 frames Cell Events: 7670 mean_circ: 0.935913
Ran for 143550 frames Cell Events: 7700 mean_circ: 0.935874
Ran for 144550 frames Cell Events: 7768 mean_circ: 0.935656
Ran for 145050 frames Cell Events: 7816 mean_circ: 0.935656
Ran for 146550 frames Cell Events: 7907 mean_circ: 0.935617
Ran for 147050 frames Cell Events: 7934 mean_circ: 0.935593



End this measurment..



Stopping time: 3.02119e+008
****************************************
Measurement finished

Mean Circularitiy: 0.935669
Mean Area: 637.924
Mean FL-1 intensity: 2755.56
Mean FL-2 intensity: 4932.79
Mean FL-3 intensity: 0

frames processed: 146371
Total Time: 74394.3ms
Number of Runs (new bg img) 0
Frames collected: 148686
Skipped frames: 88902
Rescued Frames: 4943592
Samples DAQ: 37171418
Samples written: 74342536
Samples per Channel: 37171268
FPS: 1998.62

Samples per second: 499652
Expected samples/s: 500000

Events: 8008
Events per second: 107.643
jumped back (PMT): 12
#Frames read, frequency
# 0 frames :218240
# 1 frames :66602
# 2 frames :3240
# 3 frames :981
# 4 frames :382
# 5 frames :173
# 6 frames :2915
# 7 frames :1196
# 8 frames :1232
# 9 frames :533
# 10 frames :347
# 11 frames :195
# 12 frames :206
# 13 frames :141
# 14 frames :247
# 15 frames :165
# 16 frames :115
# 17 frames :77
# 18 frames :53
# 19 frames :38
# 20 frames :55
# 21 frames :34
# 22 frames :35
# 23 frames :25
# 24 frames :15
# 25 frames :11
# 26 frames :12
# 27 frames :13
# 28 frames :12
# 29 frames :11
# 30 frames :11
# 31 frames :7
# 32 frames :3
# 33 frames :1
# 34 frames :4
# 35 frames :4
# 36 frames :3
# 37 frames :1
# 38 frames :2
# 39 frames :2
# 40 frames :2
# 41 frames :1
# 43 frames :1
# 45 frames :1
# 46 frames :2
# 47 frames :1
# 49 frames :1
# 52 frames :2
# 54 frames :1
# 58 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.4664
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0