Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.14.07 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "06b2a0ba78205a7ca5e4b9e8377f4b7c",
"name": "M1_20170518_2026_dsRNA_J6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:14:33"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.4865
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.02651e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 90 mean_circ: 0.947872
Ran for 3050 frames Cell Events: 170 mean_circ: 0.947059
Ran for 3550 frames Cell Events: 206 mean_circ: 0.94913
Ran for 4050 frames Cell Events: 233 mean_circ: 0.948654
Ran for 4550 frames Cell Events: 263 mean_circ: 0.950473
Ran for 5050 frames Cell Events: 287 mean_circ: 0.950332
Ran for 6050 frames Cell Events: 354 mean_circ: 0.95035
Ran for 8050 frames Cell Events: 463 mean_circ: 0.950411
Ran for 9050 frames Cell Events: 492 mean_circ: 0.949981
Ran for 10050 frames Cell Events: 543 mean_circ: 0.949738
Ran for 10550 frames Cell Events: 558 mean_circ: 0.949902
Ran for 11550 frames Cell Events: 611 mean_circ: 0.950346
Ran for 13550 frames Cell Events: 729 mean_circ: 0.948749
Ran for 14050 frames Cell Events: 760 mean_circ: 0.949156
Ran for 14550 frames Cell Events: 794 mean_circ: 0.948853
Ran for 15550 frames Cell Events: 847 mean_circ: 0.94862
Ran for 17550 frames Cell Events: 1005 mean_circ: 0.948752
Ran for 18550 frames Cell Events: 1061 mean_circ: 0.94869
Ran for 19050 frames Cell Events: 1092 mean_circ: 0.948516
Ran for 20550 frames Cell Events: 1178 mean_circ: 0.948609
Ran for 21050 frames Cell Events: 1203 mean_circ: 0.948436
Ran for 21550 frames Cell Events: 1244 mean_circ: 0.948358
Ran for 22050 frames Cell Events: 1286 mean_circ: 0.947898
Ran for 23050 frames Cell Events: 1352 mean_circ: 0.947967
Ran for 24050 frames Cell Events: 1419 mean_circ: 0.94789
Ran for 25050 frames Cell Events: 1477 mean_circ: 0.948134
Ran for 25550 frames Cell Events: 1494 mean_circ: 0.947996
Ran for 27050 frames Cell Events: 1566 mean_circ: 0.947874
Ran for 29550 frames Cell Events: 1735 mean_circ: 0.948012
Ran for 30050 frames Cell Events: 1774 mean_circ: 0.947824
Ran for 30550 frames Cell Events: 1793 mean_circ: 0.947803
Ran for 31050 frames Cell Events: 1813 mean_circ: 0.947818
Ran for 32050 frames Cell Events: 1855 mean_circ: 0.947727
Ran for 34550 frames Cell Events: 1974 mean_circ: 0.947845
Ran for 35050 frames Cell Events: 1988 mean_circ: 0.947896
Ran for 35550 frames Cell Events: 2008 mean_circ: 0.947925
Ran for 36050 frames Cell Events: 2045 mean_circ: 0.947986
Ran for 36550 frames Cell Events: 2077 mean_circ: 0.947986
Ran for 38050 frames Cell Events: 2165 mean_circ: 0.947887
Ran for 38550 frames Cell Events: 2179 mean_circ: 0.947884
Ran for 39050 frames Cell Events: 2216 mean_circ: 0.947979
Ran for 39550 frames Cell Events: 2248 mean_circ: 0.947851
Ran for 41050 frames Cell Events: 2336 mean_circ: 0.947826
Ran for 42050 frames Cell Events: 2392 mean_circ: 0.947788
Ran for 43050 frames Cell Events: 2444 mean_circ: 0.947675
Ran for 43550 frames Cell Events: 2472 mean_circ: 0.947645
Ran for 45050 frames Cell Events: 2559 mean_circ: 0.947539
Ran for 46050 frames Cell Events: 2627 mean_circ: 0.947391
Ran for 48550 frames Cell Events: 2783 mean_circ: 0.947186
Ran for 49050 frames Cell Events: 2820 mean_circ: 0.947298
Ran for 49550 frames Cell Events: 2857 mean_circ: 0.947316
Ran for 50050 frames Cell Events: 2877 mean_circ: 0.947218
Ran for 51050 frames Cell Events: 2953 mean_circ: 0.947529
Ran for 55050 frames Cell Events: 3229 mean_circ: 0.947405
Ran for 57050 frames Cell Events: 3349 mean_circ: 0.947251
Ran for 57550 frames Cell Events: 3377 mean_circ: 0.947357
Ran for 59050 frames Cell Events: 3471 mean_circ: 0.947325
Ran for 60050 frames Cell Events: 3528 mean_circ: 0.947499
Ran for 60550 frames Cell Events: 3551 mean_circ: 0.947538
Ran for 61050 frames Cell Events: 3577 mean_circ: 0.947595
Ran for 61550 frames Cell Events: 3616 mean_circ: 0.94764
Ran for 63550 frames Cell Events: 3759 mean_circ: 0.947467
Ran for 65550 frames Cell Events: 3932 mean_circ: 0.947621
Ran for 66050 frames Cell Events: 3979 mean_circ: 0.947621
Ran for 66550 frames Cell Events: 4016 mean_circ: 0.947152
Ran for 68550 frames Cell Events: 4188 mean_circ: 0.946701
Ran for 69050 frames Cell Events: 4214 mean_circ: 0.946735
Ran for 69550 frames Cell Events: 4251 mean_circ: 0.946764
Ran for 70550 frames Cell Events: 4336 mean_circ: 0.946814
Ran for 71050 frames Cell Events: 4385 mean_circ: 0.94686
Ran for 72050 frames Cell Events: 4438 mean_circ: 0.946844
Ran for 73050 frames Cell Events: 4504 mean_circ: 0.946821
Ran for 73550 frames Cell Events: 4541 mean_circ: 0.946831
Ran for 74550 frames Cell Events: 4625 mean_circ: 0.946823
Ran for 79050 frames Cell Events: 4940 mean_circ: 0.947043
Ran for 81550 frames Cell Events: 5123 mean_circ: 0.947199
Ran for 82550 frames Cell Events: 5204 mean_circ: 0.947197
Ran for 83050 frames Cell Events: 5237 mean_circ: 0.947197
Ran for 83550 frames Cell Events: 5259 mean_circ: 0.947286
Ran for 85050 frames Cell Events: 5356 mean_circ: 0.947368
Ran for 90050 frames Cell Events: 5707 mean_circ: 0.947275
Ran for 90550 frames Cell Events: 5742 mean_circ: 0.947275
Ran for 91550 frames Cell Events: 5814 mean_circ: 0.947268
Ran for 92050 frames Cell Events: 5845 mean_circ: 0.947247
Ran for 93550 frames Cell Events: 5958 mean_circ: 0.94722
Ran for 95550 frames Cell Events: 6107 mean_circ: 0.947156
Ran for 96050 frames Cell Events: 6153 mean_circ: 0.947185
Ran for 96550 frames Cell Events: 6186 mean_circ: 0.947195
Ran for 97050 frames Cell Events: 6236 mean_circ: 0.947208
Ran for 99050 frames Cell Events: 6410 mean_circ: 0.947204
Ran for 99550 frames Cell Events: 6437 mean_circ: 0.947193
Ran for 100550 frames Cell Events: 6504 mean_circ: 0.947219
Ran for 101050 frames Cell Events: 6550 mean_circ: 0.947219
Ran for 103050 frames Cell Events: 6669 mean_circ: 0.947212
Ran for 104550 frames Cell Events: 6767 mean_circ: 0.94725
Ran for 105550 frames Cell Events: 6829 mean_circ: 0.947289
Ran for 106550 frames Cell Events: 6891 mean_circ: 0.947178
Ran for 107050 frames Cell Events: 6925 mean_circ: 0.947158
Ran for 108050 frames Cell Events: 6983 mean_circ: 0.947199
Ran for 108550 frames Cell Events: 7017 mean_circ: 0.947149
Ran for 111050 frames Cell Events: 7195 mean_circ: 0.947116
Ran for 111550 frames Cell Events: 7222 mean_circ: 0.947146
Ran for 112050 frames Cell Events: 7245 mean_circ: 0.947089
Ran for 114050 frames Cell Events: 7371 mean_circ: 0.947028
Ran for 114550 frames Cell Events: 7394 mean_circ: 0.946995
Ran for 116050 frames Cell Events: 7491 mean_circ: 0.946978
Ran for 117050 frames Cell Events: 7566 mean_circ: 0.947026
Ran for 118050 frames Cell Events: 7633 mean_circ: 0.947056
Ran for 120550 frames Cell Events: 7807 mean_circ: 0.94716
Ran for 121050 frames Cell Events: 7831 mean_circ: 0.947113
Ran for 121550 frames Cell Events: 7851 mean_circ: 0.947122
Ran for 122550 frames Cell Events: 7898 mean_circ: 0.947213
Ran for 123550 frames Cell Events: 7978 mean_circ: 0.947144
Ran for 124050 frames Cell Events: 8016 mean_circ: 0.947147
Ran for 125050 frames Cell Events: 8079 mean_circ: 0.947137
Ran for 125550 frames Cell Events: 8108 mean_circ: 0.947139
Ran for 126550 frames Cell Events: 8179 mean_circ: 0.947125
Ran for 127050 frames Cell Events: 8216 mean_circ: 0.947121
Ran for 128050 frames Cell Events: 8262 mean_circ: 0.947064
Ran for 129050 frames Cell Events: 8319 mean_circ: 0.947074
Ran for 129550 frames Cell Events: 8350 mean_circ: 0.947059
Ran for 130050 frames Cell Events: 8370 mean_circ: 0.947061
Ran for 131050 frames Cell Events: 8447 mean_circ: 0.94712
Ran for 131550 frames Cell Events: 8479 mean_circ: 0.947099
Ran for 132050 frames Cell Events: 8497 mean_circ: 0.94709
Ran for 133050 frames Cell Events: 8553 mean_circ: 0.947085
Ran for 133550 frames Cell Events: 8603 mean_circ: 0.94711
Ran for 134050 frames Cell Events: 8633 mean_circ: 0.947057
Ran for 134550 frames Cell Events: 8662 mean_circ: 0.947053
Ran for 140050 frames Cell Events: 8990 mean_circ: 0.946999
Ran for 141550 frames Cell Events: 9107 mean_circ: 0.947023
Ran for 142550 frames Cell Events: 9168 mean_circ: 0.946996
Ran for 143050 frames Cell Events: 9192 mean_circ: 0.947014
Ran for 145050 frames Cell Events: 9331 mean_circ: 0.946997
Ran for 145550 frames Cell Events: 9368 mean_circ: 0.946989
Ran for 146050 frames Cell Events: 9401 mean_circ: 0.946948
Ran for 147050 frames Cell Events: 9472 mean_circ: 0.946916
Ran for 147550 frames Cell Events: 9498 mean_circ: 0.946918
Ran for 148550 frames Cell Events: 9555 mean_circ: 0.946957
Ran for 151550 frames Cell Events: 9791 mean_circ: 0.946921
Ran for 152050 frames Cell Events: 9818 mean_circ: 0.946922
Ran for 153550 frames Cell Events: 9935 mean_circ: 0.946947
Ran for 154550 frames Cell Events: 9986 mean_circ: 0.94698



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 3.02728e+008
****************************************
Measurement finished

Mean Circularitiy: 0.946983
Mean Area: 645.753
Mean FL-1 intensity: 3096.84
Mean FL-2 intensity: 4863.72
Mean FL-3 intensity: 0

frames processed: 153337
Total Time: 77488.1ms
Number of Runs (new bg img) 0
Frames collected: 154783
Skipped frames: 90348
Rescued Frames: 5025990
Samples DAQ: 38697784
Samples written: 77391368
Samples per Channel: 38695684
FPS: 1997.51

Samples per second: 499376
Expected samples/s: 500000

Events: 10002
Events per second: 129.078
jumped back (PMT): 3684
#Frames read, frequency
# 0 frames :238478
# 1 frames :58216
# 2 frames :2911
# 3 frames :971
# 4 frames :437
# 5 frames :191
# 6 frames :2745
# 7 frames :1001
# 8 frames :1224
# 9 frames :519
# 10 frames :346
# 11 frames :231
# 12 frames :233
# 13 frames :159
# 14 frames :387
# 15 frames :225
# 16 frames :184
# 17 frames :122
# 18 frames :83
# 19 frames :53
# 20 frames :98
# 21 frames :89
# 22 frames :70
# 23 frames :62
# 24 frames :43
# 25 frames :38
# 26 frames :33
# 27 frames :25
# 28 frames :28
# 29 frames :35
# 30 frames :18
# 31 frames :20
# 32 frames :26
# 33 frames :18
# 34 frames :11
# 35 frames :13
# 36 frames :10
# 37 frames :7
# 38 frames :4
# 39 frames :4
# 40 frames :9
# 41 frames :6
# 42 frames :9
# 43 frames :5
# 44 frames :5
# 45 frames :4
# 46 frames :5
# 47 frames :4
# 49 frames :1
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 54 frames :1
# 55 frames :2
# 58 frames :1
# 60 frames :1
# 62 frames :2
# 64 frames :1
# 65 frames :2
# 70 frames :2
# 77 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.4865
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0