Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.14.42 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d1f797fe66f63a4584412223d529d570",
"name": "M1_20170518_2029_dsRNA_J6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:15:08"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.4931
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.02832e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 36 mean_circ: 0.934142
Ran for 1050 frames Cell Events: 68 mean_circ: 0.931323
Ran for 1550 frames Cell Events: 91 mean_circ: 0.928882
Ran for 2550 frames Cell Events: 124 mean_circ: 0.928882
Ran for 3050 frames Cell Events: 158 mean_circ: 0.930704
Ran for 5050 frames Cell Events: 329 mean_circ: 0.932316
Ran for 5550 frames Cell Events: 357 mean_circ: 0.9323
Ran for 7050 frames Cell Events: 491 mean_circ: 0.932873
Ran for 7550 frames Cell Events: 531 mean_circ: 0.933194
Ran for 8550 frames Cell Events: 609 mean_circ: 0.933797
Ran for 11050 frames Cell Events: 811 mean_circ: 0.933823
Ran for 11550 frames Cell Events: 836 mean_circ: 0.933941
Ran for 12550 frames Cell Events: 930 mean_circ: 0.933535
Ran for 14050 frames Cell Events: 1032 mean_circ: 0.933975
Ran for 14550 frames Cell Events: 1055 mean_circ: 0.933354
Ran for 16050 frames Cell Events: 1167 mean_circ: 0.933607
Ran for 16550 frames Cell Events: 1194 mean_circ: 0.933638
Ran for 17050 frames Cell Events: 1224 mean_circ: 0.933657
Ran for 17550 frames Cell Events: 1257 mean_circ: 0.933801
Ran for 18050 frames Cell Events: 1291 mean_circ: 0.933868
Ran for 19550 frames Cell Events: 1410 mean_circ: 0.934181
Ran for 20550 frames Cell Events: 1479 mean_circ: 0.933776
Ran for 22050 frames Cell Events: 1586 mean_circ: 0.933393
Ran for 22550 frames Cell Events: 1614 mean_circ: 0.933165
Ran for 23050 frames Cell Events: 1635 mean_circ: 0.93327
Ran for 27050 frames Cell Events: 1910 mean_circ: 0.932812
Ran for 28050 frames Cell Events: 1973 mean_circ: 0.932984
Ran for 29550 frames Cell Events: 2093 mean_circ: 0.933071
Ran for 30050 frames Cell Events: 2127 mean_circ: 0.93303
Ran for 31050 frames Cell Events: 2184 mean_circ: 0.932768
Ran for 33050 frames Cell Events: 2298 mean_circ: 0.933044
Ran for 33550 frames Cell Events: 2331 mean_circ: 0.933239
Ran for 34050 frames Cell Events: 2357 mean_circ: 0.933202
Ran for 34550 frames Cell Events: 2380 mean_circ: 0.933138
Ran for 36050 frames Cell Events: 2486 mean_circ: 0.933133
Ran for 40050 frames Cell Events: 2766 mean_circ: 0.932865
Ran for 41050 frames Cell Events: 2836 mean_circ: 0.93281
Ran for 41550 frames Cell Events: 2869 mean_circ: 0.932856
Ran for 42050 frames Cell Events: 2903 mean_circ: 0.932938
Ran for 42550 frames Cell Events: 2938 mean_circ: 0.932906
Ran for 43550 frames Cell Events: 2994 mean_circ: 0.933011
Ran for 44550 frames Cell Events: 3069 mean_circ: 0.93308
Ran for 45550 frames Cell Events: 3140 mean_circ: 0.933269
Ran for 46050 frames Cell Events: 3178 mean_circ: 0.933149
Ran for 47050 frames Cell Events: 3256 mean_circ: 0.933366
Ran for 47550 frames Cell Events: 3288 mean_circ: 0.933434
Ran for 48050 frames Cell Events: 3312 mean_circ: 0.93331
Ran for 49550 frames Cell Events: 3421 mean_circ: 0.933392
Ran for 50050 frames Cell Events: 3444 mean_circ: 0.933488
Ran for 50550 frames Cell Events: 3469 mean_circ: 0.9335
Ran for 51050 frames Cell Events: 3497 mean_circ: 0.933293
Ran for 52050 frames Cell Events: 3558 mean_circ: 0.933471
Ran for 53050 frames Cell Events: 3641 mean_circ: 0.93333
Ran for 54050 frames Cell Events: 3722 mean_circ: 0.933218
Ran for 55050 frames Cell Events: 3772 mean_circ: 0.933316
Ran for 56050 frames Cell Events: 3851 mean_circ: 0.933263
Ran for 56550 frames Cell Events: 3880 mean_circ: 0.933308
Ran for 57050 frames Cell Events: 3905 mean_circ: 0.933314
Ran for 57550 frames Cell Events: 3933 mean_circ: 0.933413
Ran for 58550 frames Cell Events: 3988 mean_circ: 0.933405
Ran for 59050 frames Cell Events: 4012 mean_circ: 0.933483
Ran for 60050 frames Cell Events: 4077 mean_circ: 0.933489
Ran for 60550 frames Cell Events: 4108 mean_circ: 0.933595
Ran for 62050 frames Cell Events: 4211 mean_circ: 0.933653
Ran for 62550 frames Cell Events: 4238 mean_circ: 0.933776
Ran for 63550 frames Cell Events: 4305 mean_circ: 0.933854
Ran for 64550 frames Cell Events: 4354 mean_circ: 0.933852
Ran for 65550 frames Cell Events: 4411 mean_circ: 0.933755
Ran for 66050 frames Cell Events: 4429 mean_circ: 0.933798
Ran for 68550 frames Cell Events: 4590 mean_circ: 0.933721
Ran for 69550 frames Cell Events: 4657 mean_circ: 0.933851
Ran for 70050 frames Cell Events: 4692 mean_circ: 0.933943
Ran for 70550 frames Cell Events: 4719 mean_circ: 0.933901
Ran for 71550 frames Cell Events: 4807 mean_circ: 0.933802
Ran for 72550 frames Cell Events: 4880 mean_circ: 0.933944
Ran for 73050 frames Cell Events: 4902 mean_circ: 0.934012
Ran for 74050 frames Cell Events: 4982 mean_circ: 0.934066
Ran for 75550 frames Cell Events: 5097 mean_circ: 0.934059
Ran for 76050 frames Cell Events: 5127 mean_circ: 0.934169
Ran for 76550 frames Cell Events: 5162 mean_circ: 0.934114
Ran for 77050 frames Cell Events: 5194 mean_circ: 0.934095
Ran for 77550 frames Cell Events: 5222 mean_circ: 0.934111
Ran for 78050 frames Cell Events: 5259 mean_circ: 0.934049
Ran for 79050 frames Cell Events: 5324 mean_circ: 0.933899
Ran for 80550 frames Cell Events: 5435 mean_circ: 0.93405
Ran for 81550 frames Cell Events: 5500 mean_circ: 0.93415
Ran for 82550 frames Cell Events: 5572 mean_circ: 0.934128
Ran for 83050 frames Cell Events: 5606 mean_circ: 0.934176
Ran for 83550 frames Cell Events: 5644 mean_circ: 0.934113
Ran for 84550 frames Cell Events: 5696 mean_circ: 0.934184
Ran for 85550 frames Cell Events: 5769 mean_circ: 0.934224
Ran for 86050 frames Cell Events: 5803 mean_circ: 0.93421
Ran for 87550 frames Cell Events: 5891 mean_circ: 0.934138
Ran for 88050 frames Cell Events: 5921 mean_circ: 0.934072
Ran for 89050 frames Cell Events: 5978 mean_circ: 0.93417
Ran for 90050 frames Cell Events: 6039 mean_circ: 0.934188
Ran for 90550 frames Cell Events: 6061 mean_circ: 0.934223
Ran for 93550 frames Cell Events: 6247 mean_circ: 0.934207
Ran for 95050 frames Cell Events: 6320 mean_circ: 0.934178
Ran for 95550 frames Cell Events: 6355 mean_circ: 0.934169
Ran for 96050 frames Cell Events: 6382 mean_circ: 0.934172
Ran for 96550 frames Cell Events: 6409 mean_circ: 0.934227
Ran for 97050 frames Cell Events: 6433 mean_circ: 0.934176
Ran for 97550 frames Cell Events: 6451 mean_circ: 0.934184
Ran for 98050 frames Cell Events: 6485 mean_circ: 0.934125
Ran for 99550 frames Cell Events: 6600 mean_circ: 0.934086
Ran for 101050 frames Cell Events: 6715 mean_circ: 0.934188
Ran for 102050 frames Cell Events: 6787 mean_circ: 0.934164
Ran for 102550 frames Cell Events: 6810 mean_circ: 0.934154
Ran for 103050 frames Cell Events: 6832 mean_circ: 0.934146
Ran for 104050 frames Cell Events: 6900 mean_circ: 0.934125
Ran for 105050 frames Cell Events: 6978 mean_circ: 0.934164
Ran for 105550 frames Cell Events: 7018 mean_circ: 0.934164
Ran for 106550 frames Cell Events: 7100 mean_circ: 0.934119
Ran for 107050 frames Cell Events: 7130 mean_circ: 0.934128
Ran for 109050 frames Cell Events: 7238 mean_circ: 0.934224
Ran for 109550 frames Cell Events: 7266 mean_circ: 0.9342
Ran for 110050 frames Cell Events: 7287 mean_circ: 0.934164
Ran for 110550 frames Cell Events: 7318 mean_circ: 0.934166
Ran for 111550 frames Cell Events: 7373 mean_circ: 0.934129
Ran for 112050 frames Cell Events: 7394 mean_circ: 0.934039
Ran for 112550 frames Cell Events: 7426 mean_circ: 0.934029
Ran for 114550 frames Cell Events: 7590 mean_circ: 0.933945
Ran for 116050 frames Cell Events: 7707 mean_circ: 0.933936
Ran for 118050 frames Cell Events: 7852 mean_circ: 0.933944
Ran for 120550 frames Cell Events: 7976 mean_circ: 0.933877
Ran for 121550 frames Cell Events: 8053 mean_circ: 0.933926
Ran for 122050 frames Cell Events: 8078 mean_circ: 0.933936
Ran for 123550 frames Cell Events: 8184 mean_circ: 0.933929
Ran for 124550 frames Cell Events: 8251 mean_circ: 0.93392
Ran for 125050 frames Cell Events: 8297 mean_circ: 0.933861
Ran for 125550 frames Cell Events: 8333 mean_circ: 0.933847
Ran for 126050 frames Cell Events: 8381 mean_circ: 0.933836
Ran for 127050 frames Cell Events: 8471 mean_circ: 0.933779
Ran for 127550 frames Cell Events: 8501 mean_circ: 0.933801
Ran for 128050 frames Cell Events: 8526 mean_circ: 0.933795
Ran for 128550 frames Cell Events: 8562 mean_circ: 0.933787
Ran for 129050 frames Cell Events: 8599 mean_circ: 0.933733
Ran for 129550 frames Cell Events: 8637 mean_circ: 0.933724
Ran for 132050 frames Cell Events: 8818 mean_circ: 0.933671
Ran for 132550 frames Cell Events: 8845 mean_circ: 0.933654
Ran for 133050 frames Cell Events: 8884 mean_circ: 0.93367
Ran for 133550 frames Cell Events: 8905 mean_circ: 0.933673
Ran for 134050 frames Cell Events: 8929 mean_circ: 0.933684
Ran for 134550 frames Cell Events: 8951 mean_circ: 0.93365
Ran for 135050 frames Cell Events: 8979 mean_circ: 0.933635
Ran for 135550 frames Cell Events: 9004 mean_circ: 0.933641
Ran for 136050 frames Cell Events: 9027 mean_circ: 0.933583
Ran for 138050 frames Cell Events: 9127 mean_circ: 0.933578
Ran for 138550 frames Cell Events: 9158 mean_circ: 0.93359
Ran for 140550 frames Cell Events: 9323 mean_circ: 0.933592
Ran for 141050 frames Cell Events: 9351 mean_circ: 0.933607
Ran for 142050 frames Cell Events: 9406 mean_circ: 0.933652
Ran for 144050 frames Cell Events: 9552 mean_circ: 0.933705
Ran for 145550 frames Cell Events: 9654 mean_circ: 0.933643
Ran for 146050 frames Cell Events: 9679 mean_circ: 0.933636
Ran for 146550 frames Cell Events: 9699 mean_circ: 0.933636
Ran for 147550 frames Cell Events: 9742 mean_circ: 0.933685
Ran for 148550 frames Cell Events: 9813 mean_circ: 0.933643
Ran for 149050 frames Cell Events: 9851 mean_circ: 0.933647



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 3.02907e+008
****************************************
Measurement finished

Mean Circularitiy: 0.933642
Mean Area: 653.297
Mean FL-1 intensity: 2941.94
Mean FL-2 intensity: 5177.6
Mean FL-3 intensity: 0

frames processed: 149597
Total Time: 75489.1ms
Number of Runs (new bg img) 0
Frames collected: 150815
Skipped frames: 91566
Rescued Frames: 5104478
Samples DAQ: 37706653
Samples written: 75407414
Samples per Channel: 37703707
FPS: 1997.84

Samples per second: 499459
Expected samples/s: 500000

Events: 10002
Events per second: 132.496
jumped back (PMT): 10
#Frames read, frequency
# 0 frames :229684
# 1 frames :58308
# 2 frames :2760
# 3 frames :969
# 4 frames :339
# 5 frames :153
# 6 frames :3467
# 7 frames :1026
# 8 frames :1204
# 9 frames :541
# 10 frames :306
# 11 frames :235
# 12 frames :201
# 13 frames :189
# 14 frames :307
# 15 frames :193
# 16 frames :135
# 17 frames :112
# 18 frames :68
# 19 frames :73
# 20 frames :72
# 21 frames :60
# 22 frames :55
# 23 frames :43
# 24 frames :33
# 25 frames :32
# 26 frames :21
# 27 frames :24
# 28 frames :25
# 29 frames :26
# 30 frames :13
# 31 frames :15
# 32 frames :10
# 33 frames :11
# 34 frames :13
# 35 frames :13
# 36 frames :11
# 37 frames :5
# 38 frames :6
# 39 frames :6
# 40 frames :10
# 41 frames :5
# 42 frames :1
# 44 frames :2
# 45 frames :1
# 46 frames :1
# 47 frames :3
# 48 frames :5
# 50 frames :1
# 51 frames :2
# 52 frames :2
# 54 frames :1
# 61 frames :1
# 64 frames :1
# 65 frames :1
# 67 frames :1
# 68 frames :1
# 82 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.4931
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0