Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.15.46 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3ba8e3dbe74fdf7a6a402ba599e2ec79",
"name": "M1_20170518_2041_dsRNA_J7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:16:11"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.5132
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.03516e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.946522
Ran for 550 frames Cell Events: 27 mean_circ: 0.942668
Ran for 2050 frames Cell Events: 100 mean_circ: 0.937702
Ran for 2550 frames Cell Events: 124 mean_circ: 0.938174
Ran for 3050 frames Cell Events: 145 mean_circ: 0.936243
Ran for 3550 frames Cell Events: 162 mean_circ: 0.937603
Ran for 4050 frames Cell Events: 194 mean_circ: 0.938827
Ran for 5050 frames Cell Events: 215 mean_circ: 0.938
Ran for 5550 frames Cell Events: 242 mean_circ: 0.938191
Ran for 7050 frames Cell Events: 317 mean_circ: 0.934621
Ran for 7550 frames Cell Events: 344 mean_circ: 0.934188
Ran for 8050 frames Cell Events: 366 mean_circ: 0.934664
Ran for 9050 frames Cell Events: 409 mean_circ: 0.935418
Ran for 9550 frames Cell Events: 435 mean_circ: 0.935286
Ran for 10050 frames Cell Events: 465 mean_circ: 0.934866
Ran for 10550 frames Cell Events: 504 mean_circ: 0.93249
Ran for 11050 frames Cell Events: 545 mean_circ: 0.93222
Ran for 12550 frames Cell Events: 637 mean_circ: 0.931538
Ran for 13050 frames Cell Events: 661 mean_circ: 0.931166
Ran for 13550 frames Cell Events: 697 mean_circ: 0.931592
Ran for 14550 frames Cell Events: 743 mean_circ: 0.931518
Ran for 15050 frames Cell Events: 786 mean_circ: 0.931199
Ran for 15550 frames Cell Events: 809 mean_circ: 0.930915
Ran for 16050 frames Cell Events: 826 mean_circ: 0.931085
Ran for 16550 frames Cell Events: 863 mean_circ: 0.931214
Ran for 17050 frames Cell Events: 891 mean_circ: 0.931289
Ran for 18050 frames Cell Events: 933 mean_circ: 0.931014
Ran for 19550 frames Cell Events: 997 mean_circ: 0.93137
Ran for 20050 frames Cell Events: 1017 mean_circ: 0.931257
Ran for 21050 frames Cell Events: 1081 mean_circ: 0.931544
Ran for 22050 frames Cell Events: 1134 mean_circ: 0.931711
Ran for 22550 frames Cell Events: 1169 mean_circ: 0.931777
Ran for 23050 frames Cell Events: 1205 mean_circ: 0.931331
Ran for 23550 frames Cell Events: 1225 mean_circ: 0.931218
Ran for 24050 frames Cell Events: 1248 mean_circ: 0.931479
Ran for 25050 frames Cell Events: 1301 mean_circ: 0.931566
Ran for 25550 frames Cell Events: 1335 mean_circ: 0.9317
Ran for 26550 frames Cell Events: 1395 mean_circ: 0.93192
Ran for 28050 frames Cell Events: 1472 mean_circ: 0.932572
Ran for 28550 frames Cell Events: 1501 mean_circ: 0.932634
Ran for 29050 frames Cell Events: 1521 mean_circ: 0.932802
Ran for 29550 frames Cell Events: 1557 mean_circ: 0.932976
Ran for 30050 frames Cell Events: 1580 mean_circ: 0.932946
Ran for 30550 frames Cell Events: 1602 mean_circ: 0.933051
Ran for 31550 frames Cell Events: 1653 mean_circ: 0.933153
Ran for 32050 frames Cell Events: 1678 mean_circ: 0.933198
Ran for 32550 frames Cell Events: 1704 mean_circ: 0.932949
Ran for 33050 frames Cell Events: 1721 mean_circ: 0.933065
Ran for 33550 frames Cell Events: 1752 mean_circ: 0.932898
Ran for 34050 frames Cell Events: 1779 mean_circ: 0.933094
Ran for 35050 frames Cell Events: 1832 mean_circ: 0.93333
Ran for 36550 frames Cell Events: 1916 mean_circ: 0.933692
Ran for 37050 frames Cell Events: 1944 mean_circ: 0.933613
Ran for 37550 frames Cell Events: 1962 mean_circ: 0.933665
Ran for 38550 frames Cell Events: 2008 mean_circ: 0.933385
Ran for 39550 frames Cell Events: 2050 mean_circ: 0.93338
Ran for 40050 frames Cell Events: 2081 mean_circ: 0.933255
Ran for 41550 frames Cell Events: 2186 mean_circ: 0.933292
Ran for 43050 frames Cell Events: 2289 mean_circ: 0.932921
Ran for 43550 frames Cell Events: 2313 mean_circ: 0.933008
Ran for 44550 frames Cell Events: 2379 mean_circ: 0.933077
Ran for 45550 frames Cell Events: 2435 mean_circ: 0.932958
Ran for 46550 frames Cell Events: 2506 mean_circ: 0.932906
Ran for 47050 frames Cell Events: 2544 mean_circ: 0.93287
Ran for 47550 frames Cell Events: 2572 mean_circ: 0.932773
Ran for 49050 frames Cell Events: 2644 mean_circ: 0.932782
Ran for 50050 frames Cell Events: 2704 mean_circ: 0.933036
Ran for 50550 frames Cell Events: 2729 mean_circ: 0.932972
Ran for 51050 frames Cell Events: 2751 mean_circ: 0.932956
Ran for 52550 frames Cell Events: 2840 mean_circ: 0.933024
Ran for 53050 frames Cell Events: 2866 mean_circ: 0.932983
Ran for 53550 frames Cell Events: 2885 mean_circ: 0.932965
Ran for 54050 frames Cell Events: 2914 mean_circ: 0.932914
Ran for 55550 frames Cell Events: 3015 mean_circ: 0.933195
Ran for 56550 frames Cell Events: 3080 mean_circ: 0.933293
Ran for 57050 frames Cell Events: 3103 mean_circ: 0.932975
Ran for 57550 frames Cell Events: 3124 mean_circ: 0.933004
Ran for 58050 frames Cell Events: 3143 mean_circ: 0.932978
Ran for 58550 frames Cell Events: 3164 mean_circ: 0.93307
Ran for 59550 frames Cell Events: 3215 mean_circ: 0.932823
Ran for 60550 frames Cell Events: 3282 mean_circ: 0.932666
Ran for 61050 frames Cell Events: 3314 mean_circ: 0.932732
Ran for 62050 frames Cell Events: 3372 mean_circ: 0.932801
Ran for 62550 frames Cell Events: 3407 mean_circ: 0.932915
Ran for 63050 frames Cell Events: 3430 mean_circ: 0.932971
Ran for 63550 frames Cell Events: 3445 mean_circ: 0.932913
Ran for 64550 frames Cell Events: 3517 mean_circ: 0.932874
Ran for 65050 frames Cell Events: 3543 mean_circ: 0.932816
Ran for 65550 frames Cell Events: 3563 mean_circ: 0.932861
Ran for 67050 frames Cell Events: 3640 mean_circ: 0.93281
Ran for 67550 frames Cell Events: 3660 mean_circ: 0.932645
Ran for 68050 frames Cell Events: 3687 mean_circ: 0.932606
Ran for 68550 frames Cell Events: 3709 mean_circ: 0.93263
Ran for 69050 frames Cell Events: 3731 mean_circ: 0.93262
Ran for 69550 frames Cell Events: 3764 mean_circ: 0.932666
Ran for 70550 frames Cell Events: 3821 mean_circ: 0.932537
Ran for 71050 frames Cell Events: 3842 mean_circ: 0.932444
Ran for 72050 frames Cell Events: 3898 mean_circ: 0.932347
Ran for 72550 frames Cell Events: 3923 mean_circ: 0.93236
Ran for 73050 frames Cell Events: 3946 mean_circ: 0.932502
Ran for 73550 frames Cell Events: 3972 mean_circ: 0.93252
Ran for 74550 frames Cell Events: 4020 mean_circ: 0.93257
Ran for 75050 frames Cell Events: 4033 mean_circ: 0.932603
Ran for 76050 frames Cell Events: 4095 mean_circ: 0.932593
Ran for 78050 frames Cell Events: 4196 mean_circ: 0.932626
Ran for 79050 frames Cell Events: 4254 mean_circ: 0.932609
Ran for 80550 frames Cell Events: 4352 mean_circ: 0.932529
Ran for 81050 frames Cell Events: 4377 mean_circ: 0.932507
Ran for 83050 frames Cell Events: 4490 mean_circ: 0.93263
Ran for 83550 frames Cell Events: 4513 mean_circ: 0.932637
Ran for 84050 frames Cell Events: 4532 mean_circ: 0.932599
Ran for 85050 frames Cell Events: 4587 mean_circ: 0.932557
Ran for 87550 frames Cell Events: 4734 mean_circ: 0.932668
Ran for 88050 frames Cell Events: 4759 mean_circ: 0.932655
Ran for 88550 frames Cell Events: 4785 mean_circ: 0.932673
Ran for 90050 frames Cell Events: 4873 mean_circ: 0.932711
Ran for 90550 frames Cell Events: 4899 mean_circ: 0.932643
Ran for 91050 frames Cell Events: 4924 mean_circ: 0.932611
Ran for 91550 frames Cell Events: 4959 mean_circ: 0.932624
Ran for 92550 frames Cell Events: 4998 mean_circ: 0.932677
Ran for 93050 frames Cell Events: 5022 mean_circ: 0.932649
Ran for 93550 frames Cell Events: 5046 mean_circ: 0.932689
Ran for 95050 frames Cell Events: 5125 mean_circ: 0.932811
Ran for 97050 frames Cell Events: 5263 mean_circ: 0.932787
Ran for 97550 frames Cell Events: 5276 mean_circ: 0.93285
Ran for 98050 frames Cell Events: 5306 mean_circ: 0.932767
Ran for 98550 frames Cell Events: 5328 mean_circ: 0.932768
Ran for 99050 frames Cell Events: 5343 mean_circ: 0.932766
Ran for 99550 frames Cell Events: 5375 mean_circ: 0.932727
Ran for 100050 frames Cell Events: 5392 mean_circ: 0.9327
Ran for 100550 frames Cell Events: 5420 mean_circ: 0.932721
Ran for 101550 frames Cell Events: 5467 mean_circ: 0.932727
Ran for 102050 frames Cell Events: 5491 mean_circ: 0.932724
Ran for 102550 frames Cell Events: 5524 mean_circ: 0.932765
Ran for 103050 frames Cell Events: 5563 mean_circ: 0.932727
Ran for 104550 frames Cell Events: 5651 mean_circ: 0.932513
Ran for 106050 frames Cell Events: 5751 mean_circ: 0.932489
Ran for 107050 frames Cell Events: 5790 mean_circ: 0.932431
Ran for 107550 frames Cell Events: 5815 mean_circ: 0.932451
Ran for 108050 frames Cell Events: 5846 mean_circ: 0.932468
Ran for 109050 frames Cell Events: 5899 mean_circ: 0.932522
Ran for 109550 frames Cell Events: 5922 mean_circ: 0.932483
Ran for 111050 frames Cell Events: 6004 mean_circ: 0.932451
Ran for 114050 frames Cell Events: 6200 mean_circ: 0.932556
Ran for 114550 frames Cell Events: 6228 mean_circ: 0.932481
Ran for 115050 frames Cell Events: 6253 mean_circ: 0.932488
Ran for 116050 frames Cell Events: 6298 mean_circ: 0.932545
Ran for 116550 frames Cell Events: 6323 mean_circ: 0.932535
Ran for 117050 frames Cell Events: 6351 mean_circ: 0.932558
Ran for 118550 frames Cell Events: 6457 mean_circ: 0.932668
Ran for 119050 frames Cell Events: 6482 mean_circ: 0.932661
Ran for 119550 frames Cell Events: 6505 mean_circ: 0.93265
Ran for 120050 frames Cell Events: 6526 mean_circ: 0.932671
Ran for 120550 frames Cell Events: 6552 mean_circ: 0.932685
Ran for 121550 frames Cell Events: 6614 mean_circ: 0.932694
Ran for 122550 frames Cell Events: 6675 mean_circ: 0.932671
Ran for 123550 frames Cell Events: 6699 mean_circ: 0.932669
Ran for 124550 frames Cell Events: 6747 mean_circ: 0.932724
Ran for 125050 frames Cell Events: 6761 mean_circ: 0.932702
Ran for 127050 frames Cell Events: 6885 mean_circ: 0.932697
Ran for 127550 frames Cell Events: 6906 mean_circ: 0.932697
Ran for 128050 frames Cell Events: 6934 mean_circ: 0.932713
Ran for 129050 frames Cell Events: 6983 mean_circ: 0.932737
Ran for 130050 frames Cell Events: 7035 mean_circ: 0.932661
Ran for 130550 frames Cell Events: 7073 mean_circ: 0.932556
Ran for 131050 frames Cell Events: 7106 mean_circ: 0.932607
Ran for 132550 frames Cell Events: 7210 mean_circ: 0.932677
Ran for 133550 frames Cell Events: 7269 mean_circ: 0.932695
Ran for 136550 frames Cell Events: 7438 mean_circ: 0.932732
Ran for 137550 frames Cell Events: 7510 mean_circ: 0.932715
Ran for 138550 frames Cell Events: 7572 mean_circ: 0.932747
Ran for 139550 frames Cell Events: 7636 mean_circ: 0.932804
Ran for 141050 frames Cell Events: 7726 mean_circ: 0.932873
Ran for 141550 frames Cell Events: 7758 mean_circ: 0.932923
Ran for 142050 frames Cell Events: 7782 mean_circ: 0.932903
Ran for 143050 frames Cell Events: 7844 mean_circ: 0.932917
Ran for 144550 frames Cell Events: 7944 mean_circ: 0.932872
Ran for 146050 frames Cell Events: 8043 mean_circ: 0.932845
Ran for 146550 frames Cell Events: 8072 mean_circ: 0.932894



End this measurment..



Stopping time: 3.03589e+008
****************************************
Measurement finished

Mean Circularitiy: 0.932913
Mean Area: 673.818
Mean FL-1 intensity: 2998
Mean FL-2 intensity: 5443.84
Mean FL-3 intensity: 0

frames processed: 145203
Total Time: 73443.3ms
Number of Runs (new bg img) 0
Frames collected: 146680
Skipped frames: 94478
Rescued Frames: 5246579
Samples DAQ: 36671584
Samples written: 73340158
Samples per Channel: 36670079
FPS: 1997.19

Samples per second: 499298
Expected samples/s: 500000

Events: 8079
Events per second: 110.003
jumped back (PMT): 12
#Frames read, frequency
# 0 frames :217132
# 1 frames :64097
# 2 frames :3028
# 3 frames :872
# 4 frames :461
# 5 frames :185
# 6 frames :2716
# 7 frames :1065
# 8 frames :1161
# 9 frames :461
# 10 frames :388
# 11 frames :259
# 12 frames :218
# 13 frames :137
# 14 frames :248
# 15 frames :165
# 16 frames :125
# 17 frames :96
# 18 frames :46
# 19 frames :43
# 20 frames :64
# 21 frames :47
# 22 frames :54
# 23 frames :25
# 24 frames :33
# 25 frames :25
# 26 frames :18
# 27 frames :18
# 28 frames :8
# 29 frames :18
# 30 frames :8
# 31 frames :6
# 32 frames :7
# 33 frames :11
# 34 frames :6
# 35 frames :2
# 36 frames :5
# 37 frames :8
# 38 frames :6
# 40 frames :4
# 42 frames :2
# 43 frames :1
# 44 frames :4
# 45 frames :1
# 47 frames :3
# 48 frames :4
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 58 frames :1
# 59 frames :2
# 79 frames :1
# 97 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.5132
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0