Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.26.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "324d9a92893385bdb6746776a8a94e13",
"name": "M1_20170519_1419_dsRNA_K6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:27:05"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9055 (max 9056) in '9055'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.9109
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.66989e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 26 mean_circ: 0.949898
Ran for 1550 frames Cell Events: 99 mean_circ: 0.941945
Ran for 2550 frames Cell Events: 169 mean_circ: 0.944634
Ran for 3050 frames Cell Events: 202 mean_circ: 0.944216
Ran for 4550 frames Cell Events: 260 mean_circ: 0.943917
Ran for 5050 frames Cell Events: 287 mean_circ: 0.945868
Ran for 5550 frames Cell Events: 319 mean_circ: 0.945085
Ran for 6050 frames Cell Events: 357 mean_circ: 0.945085
Ran for 7050 frames Cell Events: 405 mean_circ: 0.942683
Ran for 8050 frames Cell Events: 460 mean_circ: 0.942108
Ran for 8550 frames Cell Events: 484 mean_circ: 0.942861
Ran for 10050 frames Cell Events: 584 mean_circ: 0.944128
Ran for 11050 frames Cell Events: 651 mean_circ: 0.943894
Ran for 11550 frames Cell Events: 682 mean_circ: 0.943826
Ran for 12550 frames Cell Events: 746 mean_circ: 0.943955
Ran for 13050 frames Cell Events: 777 mean_circ: 0.944178
Ran for 14050 frames Cell Events: 846 mean_circ: 0.944152
Ran for 15050 frames Cell Events: 910 mean_circ: 0.944495
Ran for 15550 frames Cell Events: 936 mean_circ: 0.944073
Ran for 16550 frames Cell Events: 992 mean_circ: 0.944121
Ran for 17550 frames Cell Events: 1056 mean_circ: 0.944407
Ran for 18550 frames Cell Events: 1117 mean_circ: 0.944128
Ran for 19050 frames Cell Events: 1135 mean_circ: 0.944126
Ran for 19550 frames Cell Events: 1163 mean_circ: 0.944114
Ran for 20050 frames Cell Events: 1192 mean_circ: 0.944245
Ran for 20550 frames Cell Events: 1226 mean_circ: 0.943968
Ran for 21050 frames Cell Events: 1257 mean_circ: 0.943865
Ran for 21550 frames Cell Events: 1285 mean_circ: 0.943566
Ran for 22050 frames Cell Events: 1311 mean_circ: 0.943155
Ran for 22550 frames Cell Events: 1337 mean_circ: 0.94305
Ran for 23550 frames Cell Events: 1403 mean_circ: 0.94293
Ran for 24050 frames Cell Events: 1424 mean_circ: 0.942918
Ran for 24550 frames Cell Events: 1467 mean_circ: 0.942945
Ran for 26050 frames Cell Events: 1554 mean_circ: 0.942826
Ran for 27050 frames Cell Events: 1629 mean_circ: 0.942467
Ran for 29550 frames Cell Events: 1756 mean_circ: 0.942078
Ran for 31050 frames Cell Events: 1857 mean_circ: 0.941703
Ran for 31550 frames Cell Events: 1880 mean_circ: 0.941868
Ran for 32050 frames Cell Events: 1918 mean_circ: 0.941733
Ran for 33050 frames Cell Events: 1956 mean_circ: 0.941735
Ran for 33550 frames Cell Events: 1986 mean_circ: 0.941895
Ran for 34050 frames Cell Events: 2015 mean_circ: 0.941879
Ran for 35550 frames Cell Events: 2103 mean_circ: 0.941755
Ran for 36550 frames Cell Events: 2164 mean_circ: 0.941708
Ran for 37050 frames Cell Events: 2199 mean_circ: 0.941446
Ran for 38050 frames Cell Events: 2268 mean_circ: 0.941448
Ran for 39050 frames Cell Events: 2327 mean_circ: 0.94164
Ran for 39550 frames Cell Events: 2353 mean_circ: 0.941737
Ran for 40050 frames Cell Events: 2381 mean_circ: 0.941807
Ran for 41050 frames Cell Events: 2443 mean_circ: 0.941731
Ran for 43050 frames Cell Events: 2564 mean_circ: 0.941791
Ran for 44050 frames Cell Events: 2614 mean_circ: 0.941507
Ran for 44550 frames Cell Events: 2644 mean_circ: 0.941578
Ran for 45550 frames Cell Events: 2710 mean_circ: 0.941713
Ran for 46550 frames Cell Events: 2768 mean_circ: 0.941723
Ran for 48050 frames Cell Events: 2854 mean_circ: 0.941762
Ran for 48550 frames Cell Events: 2883 mean_circ: 0.941716
Ran for 49050 frames Cell Events: 2898 mean_circ: 0.94176
Ran for 49550 frames Cell Events: 2923 mean_circ: 0.941783
Ran for 50050 frames Cell Events: 2949 mean_circ: 0.941846
Ran for 51550 frames Cell Events: 3041 mean_circ: 0.941792
Ran for 52050 frames Cell Events: 3066 mean_circ: 0.941824
Ran for 52550 frames Cell Events: 3091 mean_circ: 0.941789
Ran for 53050 frames Cell Events: 3113 mean_circ: 0.941864
Ran for 53550 frames Cell Events: 3136 mean_circ: 0.941902
Ran for 54550 frames Cell Events: 3197 mean_circ: 0.941889
Ran for 55050 frames Cell Events: 3217 mean_circ: 0.941836
Ran for 55550 frames Cell Events: 3249 mean_circ: 0.941752
Ran for 56050 frames Cell Events: 3279 mean_circ: 0.941806
Ran for 56550 frames Cell Events: 3305 mean_circ: 0.941832
Ran for 57050 frames Cell Events: 3331 mean_circ: 0.94185
Ran for 58050 frames Cell Events: 3404 mean_circ: 0.941796
Ran for 59550 frames Cell Events: 3509 mean_circ: 0.941719
Ran for 60550 frames Cell Events: 3582 mean_circ: 0.941727
Ran for 61050 frames Cell Events: 3618 mean_circ: 0.941751
Ran for 61550 frames Cell Events: 3662 mean_circ: 0.94166
Ran for 62050 frames Cell Events: 3711 mean_circ: 0.941685
Ran for 63050 frames Cell Events: 3774 mean_circ: 0.941449
Ran for 63550 frames Cell Events: 3804 mean_circ: 0.941441
Ran for 64050 frames Cell Events: 3828 mean_circ: 0.941278
Ran for 65550 frames Cell Events: 3902 mean_circ: 0.941322
Ran for 66050 frames Cell Events: 3934 mean_circ: 0.94141
Ran for 67050 frames Cell Events: 3985 mean_circ: 0.941476
Ran for 69550 frames Cell Events: 4102 mean_circ: 0.941624
Ran for 72050 frames Cell Events: 4293 mean_circ: 0.941765
Ran for 72550 frames Cell Events: 4325 mean_circ: 0.941719
Ran for 73050 frames Cell Events: 4350 mean_circ: 0.941769
Ran for 74550 frames Cell Events: 4400 mean_circ: 0.941693
Ran for 75050 frames Cell Events: 4434 mean_circ: 0.941715
Ran for 76050 frames Cell Events: 4478 mean_circ: 0.941557
Ran for 76550 frames Cell Events: 4504 mean_circ: 0.94157
Ran for 77050 frames Cell Events: 4533 mean_circ: 0.94162
Ran for 77550 frames Cell Events: 4553 mean_circ: 0.941654
Ran for 78050 frames Cell Events: 4574 mean_circ: 0.941707
Ran for 78550 frames Cell Events: 4607 mean_circ: 0.941728
Ran for 80550 frames Cell Events: 4741 mean_circ: 0.941805
Ran for 82550 frames Cell Events: 4849 mean_circ: 0.941643
Ran for 83050 frames Cell Events: 4878 mean_circ: 0.941675
Ran for 83550 frames Cell Events: 4906 mean_circ: 0.941661
Ran for 85050 frames Cell Events: 4977 mean_circ: 0.941746
Ran for 86050 frames Cell Events: 5043 mean_circ: 0.941731
Ran for 89550 frames Cell Events: 5250 mean_circ: 0.941541
Ran for 91050 frames Cell Events: 5346 mean_circ: 0.94161
Ran for 92550 frames Cell Events: 5444 mean_circ: 0.94156
Ran for 93050 frames Cell Events: 5472 mean_circ: 0.941503
Ran for 93550 frames Cell Events: 5508 mean_circ: 0.941468
Ran for 94050 frames Cell Events: 5540 mean_circ: 0.941421
Ran for 95550 frames Cell Events: 5626 mean_circ: 0.941454
Ran for 96550 frames Cell Events: 5708 mean_circ: 0.941307
Ran for 97050 frames Cell Events: 5740 mean_circ: 0.94138
Ran for 97550 frames Cell Events: 5781 mean_circ: 0.941346
Ran for 98050 frames Cell Events: 5802 mean_circ: 0.94135
Ran for 98550 frames Cell Events: 5834 mean_circ: 0.941276
Ran for 99550 frames Cell Events: 5902 mean_circ: 0.941278
Ran for 100550 frames Cell Events: 5958 mean_circ: 0.941261
Ran for 101050 frames Cell Events: 6004 mean_circ: 0.9413
Ran for 102050 frames Cell Events: 6070 mean_circ: 0.941308
Ran for 104050 frames Cell Events: 6224 mean_circ: 0.941285
Ran for 104550 frames Cell Events: 6261 mean_circ: 0.941318
Ran for 105050 frames Cell Events: 6282 mean_circ: 0.941334
Ran for 105550 frames Cell Events: 6299 mean_circ: 0.941323
Ran for 107050 frames Cell Events: 6387 mean_circ: 0.941262
Ran for 107550 frames Cell Events: 6426 mean_circ: 0.941247
Ran for 108550 frames Cell Events: 6492 mean_circ: 0.941295
Ran for 109550 frames Cell Events: 6556 mean_circ: 0.941269
Ran for 110050 frames Cell Events: 6587 mean_circ: 0.941259
Ran for 110550 frames Cell Events: 6623 mean_circ: 0.941271
Ran for 111550 frames Cell Events: 6685 mean_circ: 0.941295
Ran for 113550 frames Cell Events: 6803 mean_circ: 0.941212
Ran for 116050 frames Cell Events: 6949 mean_circ: 0.941123
Ran for 116550 frames Cell Events: 6982 mean_circ: 0.941117
Ran for 117050 frames Cell Events: 7018 mean_circ: 0.941172
Ran for 118550 frames Cell Events: 7109 mean_circ: 0.941181
Ran for 119050 frames Cell Events: 7146 mean_circ: 0.941221
Ran for 120550 frames Cell Events: 7242 mean_circ: 0.941232
Ran for 121550 frames Cell Events: 7299 mean_circ: 0.941218
Ran for 122050 frames Cell Events: 7330 mean_circ: 0.941162
Ran for 122550 frames Cell Events: 7358 mean_circ: 0.941182
Ran for 123050 frames Cell Events: 7390 mean_circ: 0.941205
Ran for 123550 frames Cell Events: 7412 mean_circ: 0.941182
Ran for 126550 frames Cell Events: 7630 mean_circ: 0.941276
Ran for 128050 frames Cell Events: 7720 mean_circ: 0.941257
Ran for 129550 frames Cell Events: 7826 mean_circ: 0.941242
Ran for 130550 frames Cell Events: 7887 mean_circ: 0.941297
Ran for 131050 frames Cell Events: 7915 mean_circ: 0.941268
Ran for 131550 frames Cell Events: 7947 mean_circ: 0.941256
Ran for 133050 frames Cell Events: 8053 mean_circ: 0.941267
Ran for 134050 frames Cell Events: 8115 mean_circ: 0.9412
Ran for 134550 frames Cell Events: 8140 mean_circ: 0.941213
Ran for 135550 frames Cell Events: 8202 mean_circ: 0.941198
Ran for 136050 frames Cell Events: 8232 mean_circ: 0.941216
Ran for 136550 frames Cell Events: 8261 mean_circ: 0.941224
Ran for 139050 frames Cell Events: 8433 mean_circ: 0.941127
Ran for 140050 frames Cell Events: 8500 mean_circ: 0.94115
Ran for 140550 frames Cell Events: 8528 mean_circ: 0.941155
Ran for 141050 frames Cell Events: 8561 mean_circ: 0.941143
Ran for 141550 frames Cell Events: 8593 mean_circ: 0.941147
Ran for 143050 frames Cell Events: 8705 mean_circ: 0.941039
Ran for 143550 frames Cell Events: 8751 mean_circ: 0.941048
Ran for 145050 frames Cell Events: 8851 mean_circ: 0.941005
Ran for 146050 frames Cell Events: 8915 mean_circ: 0.94109
Ran for 146550 frames Cell Events: 8947 mean_circ: 0.941089
Ran for 147550 frames Cell Events: 9013 mean_circ: 0.941135
Ran for 148050 frames Cell Events: 9051 mean_circ: 0.941145



End this measurment..



Stopping time: 3.67063e+008
****************************************
Measurement finished

Mean Circularitiy: 0.941158
Mean Area: 575.995
Mean FL-1 intensity: 2941.29
Mean FL-2 intensity: 4508.23
Mean FL-3 intensity: 0

frames processed: 144442
Total Time: 74138.9ms
Number of Runs (new bg img) 0
Frames collected: 148110
Skipped frames: 23172
Rescued Frames: 1193184
Samples DAQ: 37028116
Samples written: 74054986
Samples per Channel: 37027493
FPS: 1997.74

Samples per second: 499434
Expected samples/s: 500000

Events: 9056
Events per second: 122.149
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :227613
# 1 frames :55947
# 2 frames :2887
# 3 frames :958
# 4 frames :439
# 5 frames :185
# 6 frames :2699
# 7 frames :1074
# 8 frames :1209
# 9 frames :598
# 10 frames :463
# 11 frames :245
# 12 frames :235
# 13 frames :171
# 14 frames :292
# 15 frames :193
# 16 frames :161
# 17 frames :109
# 18 frames :75
# 19 frames :48
# 20 frames :83
# 21 frames :67
# 22 frames :60
# 23 frames :48
# 24 frames :47
# 25 frames :21
# 26 frames :23
# 27 frames :14
# 28 frames :21
# 29 frames :11
# 30 frames :13
# 31 frames :12
# 32 frames :4
# 33 frames :10
# 34 frames :9
# 35 frames :11
# 36 frames :7
# 37 frames :4
# 38 frames :4
# 39 frames :2
# 40 frames :1
# 41 frames :3
# 42 frames :3
# 43 frames :2
# 44 frames :3
# 46 frames :3
# 48 frames :2
# 49 frames :1
# 58 frames :1
# 61 frames :2
# 65 frames :1
# 67 frames :1
# 68 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.9109
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0