Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.30.52 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5006bb7e141270ded658892ca94a7f77",
"name": "M1_20170519_1502_dsRNA_L2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:31:23"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8853 (max 8854) in '8853'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.4996
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.69616e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 27 mean_circ: 0.920594
Ran for 1050 frames Cell Events: 43 mean_circ: 0.939578
Ran for 2050 frames Cell Events: 99 mean_circ: 0.942482
Ran for 3050 frames Cell Events: 145 mean_circ: 0.943648
Ran for 4050 frames Cell Events: 210 mean_circ: 0.942413
Ran for 4550 frames Cell Events: 239 mean_circ: 0.942632
Ran for 5050 frames Cell Events: 266 mean_circ: 0.9419
Ran for 9050 frames Cell Events: 450 mean_circ: 0.94088
Ran for 10050 frames Cell Events: 497 mean_circ: 0.941367
Ran for 11050 frames Cell Events: 538 mean_circ: 0.940905
Ran for 11550 frames Cell Events: 565 mean_circ: 0.940373
Ran for 12050 frames Cell Events: 589 mean_circ: 0.940334
Ran for 13050 frames Cell Events: 646 mean_circ: 0.939243
Ran for 13550 frames Cell Events: 679 mean_circ: 0.939806
Ran for 14550 frames Cell Events: 722 mean_circ: 0.939948
Ran for 15050 frames Cell Events: 748 mean_circ: 0.939353
Ran for 15550 frames Cell Events: 772 mean_circ: 0.938767
Ran for 17050 frames Cell Events: 847 mean_circ: 0.939581
Ran for 17550 frames Cell Events: 873 mean_circ: 0.939112
Ran for 18550 frames Cell Events: 926 mean_circ: 0.939758
Ran for 19550 frames Cell Events: 996 mean_circ: 0.940099
Ran for 21050 frames Cell Events: 1065 mean_circ: 0.940464
Ran for 22050 frames Cell Events: 1114 mean_circ: 0.940188
Ran for 22550 frames Cell Events: 1136 mean_circ: 0.940388
Ran for 23550 frames Cell Events: 1191 mean_circ: 0.940278
Ran for 24050 frames Cell Events: 1217 mean_circ: 0.940437
Ran for 25050 frames Cell Events: 1281 mean_circ: 0.940551
Ran for 25550 frames Cell Events: 1309 mean_circ: 0.940523
Ran for 26050 frames Cell Events: 1352 mean_circ: 0.940523
Ran for 27050 frames Cell Events: 1401 mean_circ: 0.940099
Ran for 28550 frames Cell Events: 1467 mean_circ: 0.940452
Ran for 29550 frames Cell Events: 1526 mean_circ: 0.940569
Ran for 30550 frames Cell Events: 1571 mean_circ: 0.940623
Ran for 32550 frames Cell Events: 1689 mean_circ: 0.940737
Ran for 33550 frames Cell Events: 1737 mean_circ: 0.940163
Ran for 34550 frames Cell Events: 1798 mean_circ: 0.940369
Ran for 36050 frames Cell Events: 1867 mean_circ: 0.940293
Ran for 37050 frames Cell Events: 1932 mean_circ: 0.940224
Ran for 37550 frames Cell Events: 1953 mean_circ: 0.94032
Ran for 38550 frames Cell Events: 2004 mean_circ: 0.940271
Ran for 39050 frames Cell Events: 2027 mean_circ: 0.94024
Ran for 41550 frames Cell Events: 2184 mean_circ: 0.939993
Ran for 42050 frames Cell Events: 2204 mean_circ: 0.940124
Ran for 43550 frames Cell Events: 2297 mean_circ: 0.939796
Ran for 44050 frames Cell Events: 2322 mean_circ: 0.939926
Ran for 45050 frames Cell Events: 2378 mean_circ: 0.940125
Ran for 45550 frames Cell Events: 2409 mean_circ: 0.940217
Ran for 47050 frames Cell Events: 2478 mean_circ: 0.940248
Ran for 47550 frames Cell Events: 2509 mean_circ: 0.940339
Ran for 48050 frames Cell Events: 2525 mean_circ: 0.940446
Ran for 49050 frames Cell Events: 2594 mean_circ: 0.940514
Ran for 50550 frames Cell Events: 2685 mean_circ: 0.940446
Ran for 52050 frames Cell Events: 2777 mean_circ: 0.940641
Ran for 52550 frames Cell Events: 2804 mean_circ: 0.940608
Ran for 53050 frames Cell Events: 2830 mean_circ: 0.940564
Ran for 53550 frames Cell Events: 2863 mean_circ: 0.940579
Ran for 54550 frames Cell Events: 2912 mean_circ: 0.940586
Ran for 55550 frames Cell Events: 2972 mean_circ: 0.940748
Ran for 59550 frames Cell Events: 3211 mean_circ: 0.940919
Ran for 61050 frames Cell Events: 3305 mean_circ: 0.940999
Ran for 61550 frames Cell Events: 3326 mean_circ: 0.941076
Ran for 62050 frames Cell Events: 3359 mean_circ: 0.94101
Ran for 62550 frames Cell Events: 3384 mean_circ: 0.940956
Ran for 63050 frames Cell Events: 3417 mean_circ: 0.941052
Ran for 64550 frames Cell Events: 3506 mean_circ: 0.940931
Ran for 66050 frames Cell Events: 3612 mean_circ: 0.940927
Ran for 66550 frames Cell Events: 3638 mean_circ: 0.940941
Ran for 68550 frames Cell Events: 3755 mean_circ: 0.940948
Ran for 69050 frames Cell Events: 3779 mean_circ: 0.940913
Ran for 70050 frames Cell Events: 3835 mean_circ: 0.940956
Ran for 70550 frames Cell Events: 3862 mean_circ: 0.940887
Ran for 71550 frames Cell Events: 3915 mean_circ: 0.940899
Ran for 74050 frames Cell Events: 4052 mean_circ: 0.940885
Ran for 74550 frames Cell Events: 4076 mean_circ: 0.940864
Ran for 75050 frames Cell Events: 4100 mean_circ: 0.940791
Ran for 75550 frames Cell Events: 4128 mean_circ: 0.94086
Ran for 76050 frames Cell Events: 4150 mean_circ: 0.940819
Ran for 76550 frames Cell Events: 4173 mean_circ: 0.940806
Ran for 78050 frames Cell Events: 4249 mean_circ: 0.940852
Ran for 78550 frames Cell Events: 4281 mean_circ: 0.940846
Ran for 79050 frames Cell Events: 4304 mean_circ: 0.940914
Ran for 79550 frames Cell Events: 4318 mean_circ: 0.940839
Ran for 80050 frames Cell Events: 4340 mean_circ: 0.940846
Ran for 81050 frames Cell Events: 4382 mean_circ: 0.940873
Ran for 81550 frames Cell Events: 4408 mean_circ: 0.940942
Ran for 83050 frames Cell Events: 4480 mean_circ: 0.940972
Ran for 84050 frames Cell Events: 4540 mean_circ: 0.940786
Ran for 84550 frames Cell Events: 4563 mean_circ: 0.940766
Ran for 85050 frames Cell Events: 4590 mean_circ: 0.940805
Ran for 87550 frames Cell Events: 4750 mean_circ: 0.940782
Ran for 88550 frames Cell Events: 4805 mean_circ: 0.940826
Ran for 90550 frames Cell Events: 4911 mean_circ: 0.940701
Ran for 91050 frames Cell Events: 4950 mean_circ: 0.940579
Ran for 91550 frames Cell Events: 4973 mean_circ: 0.940533
Ran for 92050 frames Cell Events: 5005 mean_circ: 0.940588
Ran for 92550 frames Cell Events: 5030 mean_circ: 0.940608
Ran for 93050 frames Cell Events: 5061 mean_circ: 0.940661
Ran for 93550 frames Cell Events: 5085 mean_circ: 0.940749
Ran for 94550 frames Cell Events: 5165 mean_circ: 0.940733
Ran for 96050 frames Cell Events: 5251 mean_circ: 0.94076
Ran for 96550 frames Cell Events: 5282 mean_circ: 0.940741
Ran for 98050 frames Cell Events: 5373 mean_circ: 0.940593
Ran for 102050 frames Cell Events: 5635 mean_circ: 0.940358
Ran for 102550 frames Cell Events: 5659 mean_circ: 0.94028
Ran for 104050 frames Cell Events: 5755 mean_circ: 0.940195
Ran for 105550 frames Cell Events: 5856 mean_circ: 0.940184
Ran for 106050 frames Cell Events: 5894 mean_circ: 0.94015
Ran for 106550 frames Cell Events: 5926 mean_circ: 0.939895
Ran for 107550 frames Cell Events: 5973 mean_circ: 0.939956
Ran for 108050 frames Cell Events: 5998 mean_circ: 0.939953
Ran for 108550 frames Cell Events: 6032 mean_circ: 0.93997
Ran for 109550 frames Cell Events: 6095 mean_circ: 0.939998
Ran for 111050 frames Cell Events: 6206 mean_circ: 0.939879
Ran for 111550 frames Cell Events: 6236 mean_circ: 0.939875
Ran for 112050 frames Cell Events: 6273 mean_circ: 0.939884
Ran for 112550 frames Cell Events: 6306 mean_circ: 0.939874
Ran for 113050 frames Cell Events: 6337 mean_circ: 0.939874
Ran for 113550 frames Cell Events: 6371 mean_circ: 0.939877
Ran for 115050 frames Cell Events: 6458 mean_circ: 0.939873
Ran for 115550 frames Cell Events: 6475 mean_circ: 0.939852
Ran for 116050 frames Cell Events: 6513 mean_circ: 0.939777
Ran for 117050 frames Cell Events: 6566 mean_circ: 0.939773
Ran for 118050 frames Cell Events: 6638 mean_circ: 0.939903
Ran for 118550 frames Cell Events: 6667 mean_circ: 0.939923
Ran for 121050 frames Cell Events: 6817 mean_circ: 0.939821
Ran for 122050 frames Cell Events: 6874 mean_circ: 0.939756
Ran for 123550 frames Cell Events: 6981 mean_circ: 0.939771
Ran for 124550 frames Cell Events: 7036 mean_circ: 0.939763
Ran for 125050 frames Cell Events: 7052 mean_circ: 0.939758
Ran for 125550 frames Cell Events: 7079 mean_circ: 0.93974
Ran for 126050 frames Cell Events: 7104 mean_circ: 0.939716
Ran for 126550 frames Cell Events: 7135 mean_circ: 0.939741
Ran for 127050 frames Cell Events: 7173 mean_circ: 0.939647
Ran for 128050 frames Cell Events: 7240 mean_circ: 0.939609
Ran for 128550 frames Cell Events: 7262 mean_circ: 0.939605
Ran for 129550 frames Cell Events: 7312 mean_circ: 0.939598
Ran for 130050 frames Cell Events: 7338 mean_circ: 0.939606
Ran for 130550 frames Cell Events: 7356 mean_circ: 0.939591
Ran for 131550 frames Cell Events: 7421 mean_circ: 0.93954
Ran for 132050 frames Cell Events: 7437 mean_circ: 0.939551
Ran for 133050 frames Cell Events: 7479 mean_circ: 0.939514
Ran for 133550 frames Cell Events: 7503 mean_circ: 0.939541
Ran for 134050 frames Cell Events: 7531 mean_circ: 0.939503
Ran for 134550 frames Cell Events: 7551 mean_circ: 0.939446
Ran for 135050 frames Cell Events: 7571 mean_circ: 0.939463
Ran for 136050 frames Cell Events: 7645 mean_circ: 0.939442
Ran for 136550 frames Cell Events: 7677 mean_circ: 0.939435
Ran for 138050 frames Cell Events: 7764 mean_circ: 0.939449
Ran for 139550 frames Cell Events: 7889 mean_circ: 0.939504
Ran for 141550 frames Cell Events: 8041 mean_circ: 0.939447
Ran for 143050 frames Cell Events: 8129 mean_circ: 0.939449
Ran for 143550 frames Cell Events: 8151 mean_circ: 0.939417
Ran for 144550 frames Cell Events: 8203 mean_circ: 0.939443
Ran for 145050 frames Cell Events: 8235 mean_circ: 0.939469
Ran for 147050 frames Cell Events: 8354 mean_circ: 0.939396
Ran for 147550 frames Cell Events: 8378 mean_circ: 0.939432
Ran for 148050 frames Cell Events: 8404 mean_circ: 0.939444
Ran for 149050 frames Cell Events: 8468 mean_circ: 0.939376
Ran for 150050 frames Cell Events: 8537 mean_circ: 0.939418
Ran for 151050 frames Cell Events: 8576 mean_circ: 0.939375
Ran for 152050 frames Cell Events: 8599 mean_circ: 0.939391
Ran for 152550 frames Cell Events: 8637 mean_circ: 0.93945
Ran for 154050 frames Cell Events: 8746 mean_circ: 0.93947
Ran for 154550 frames Cell Events: 8781 mean_circ: 0.93947
Ran for 155050 frames Cell Events: 8825 mean_circ: 0.939469



End this measurment..



Stopping time: 3.69694e+008
****************************************
Measurement finished

Mean Circularitiy: 0.939506
Mean Area: 596.294
Mean FL-1 intensity: 3170.05
Mean FL-2 intensity: 4222.52
Mean FL-3 intensity: 0

frames processed: 154214
Total Time: 77894.4ms
Number of Runs (new bg img) 0
Frames collected: 155629
Skipped frames: 37718
Rescued Frames: 1810004
Samples DAQ: 38907193
Samples written: 77814236
Samples per Channel: 38907118
FPS: 1997.95

Samples per second: 499485
Expected samples/s: 500000

Events: 8854
Events per second: 113.667
jumped back (PMT): 4010
#Frames read, frequency
# 0 frames :233212
# 1 frames :65006
# 2 frames :3357
# 3 frames :961
# 4 frames :472
# 5 frames :238
# 6 frames :2503
# 7 frames :1337
# 8 frames :1194
# 9 frames :565
# 10 frames :387
# 11 frames :194
# 12 frames :254
# 13 frames :148
# 14 frames :291
# 15 frames :207
# 16 frames :140
# 17 frames :122
# 18 frames :81
# 19 frames :61
# 20 frames :53
# 21 frames :77
# 22 frames :53
# 23 frames :51
# 24 frames :44
# 25 frames :28
# 26 frames :22
# 27 frames :23
# 28 frames :12
# 29 frames :16
# 30 frames :14
# 31 frames :13
# 32 frames :11
# 33 frames :11
# 34 frames :6
# 35 frames :7
# 36 frames :3
# 37 frames :5
# 38 frames :5
# 39 frames :3
# 40 frames :4
# 41 frames :4
# 42 frames :4
# 43 frames :2
# 44 frames :5
# 45 frames :2
# 48 frames :3
# 49 frames :1
# 52 frames :1
# 55 frames :1
# 57 frames :1
# 59 frames :1
# 60 frames :1
# 73 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.4996
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0