Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.31.32 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bb3eec7a0dda4aac3d4ccce35d6d0d81",
"name": "M1_20170519_1505_dsRNA_L2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:32:01"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8920 (max 8921) in '8920'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.4754
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.69778e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2550 frames Cell Events: 230 mean_circ: 0.92523
Ran for 3050 frames Cell Events: 255 mean_circ: 0.9275
Ran for 3550 frames Cell Events: 283 mean_circ: 0.927963
Ran for 4550 frames Cell Events: 304 mean_circ: 0.928137
Ran for 5050 frames Cell Events: 325 mean_circ: 0.928023
Ran for 5550 frames Cell Events: 348 mean_circ: 0.929129
Ran for 6550 frames Cell Events: 397 mean_circ: 0.929263
Ran for 9550 frames Cell Events: 627 mean_circ: 0.927661
Ran for 10050 frames Cell Events: 662 mean_circ: 0.927661
Ran for 10550 frames Cell Events: 692 mean_circ: 0.928205
Ran for 11050 frames Cell Events: 721 mean_circ: 0.929024
Ran for 11550 frames Cell Events: 753 mean_circ: 0.928879
Ran for 13550 frames Cell Events: 881 mean_circ: 0.928608
Ran for 14050 frames Cell Events: 920 mean_circ: 0.928608
Ran for 15550 frames Cell Events: 1001 mean_circ: 0.928976
Ran for 16050 frames Cell Events: 1015 mean_circ: 0.929008
Ran for 16550 frames Cell Events: 1055 mean_circ: 0.928666
Ran for 18050 frames Cell Events: 1178 mean_circ: 0.928234
Ran for 20050 frames Cell Events: 1292 mean_circ: 0.928354
Ran for 21550 frames Cell Events: 1394 mean_circ: 0.928726
Ran for 22050 frames Cell Events: 1414 mean_circ: 0.928726
Ran for 22550 frames Cell Events: 1436 mean_circ: 0.928585
Ran for 23550 frames Cell Events: 1502 mean_circ: 0.92879
Ran for 24050 frames Cell Events: 1533 mean_circ: 0.928727
Ran for 26050 frames Cell Events: 1657 mean_circ: 0.928808
Ran for 26550 frames Cell Events: 1686 mean_circ: 0.928959
Ran for 28550 frames Cell Events: 1841 mean_circ: 0.929132
Ran for 29050 frames Cell Events: 1857 mean_circ: 0.929206
Ran for 30050 frames Cell Events: 1908 mean_circ: 0.929137
Ran for 30550 frames Cell Events: 1941 mean_circ: 0.929226
Ran for 31050 frames Cell Events: 1967 mean_circ: 0.929145
Ran for 32050 frames Cell Events: 2040 mean_circ: 0.929305
Ran for 32550 frames Cell Events: 2065 mean_circ: 0.929308
Ran for 33050 frames Cell Events: 2092 mean_circ: 0.929331
Ran for 33550 frames Cell Events: 2121 mean_circ: 0.929388
Ran for 34050 frames Cell Events: 2152 mean_circ: 0.929284
Ran for 34550 frames Cell Events: 2188 mean_circ: 0.929284
Ran for 35050 frames Cell Events: 2224 mean_circ: 0.929486
Ran for 35550 frames Cell Events: 2245 mean_circ: 0.929455
Ran for 36050 frames Cell Events: 2267 mean_circ: 0.929427
Ran for 36550 frames Cell Events: 2301 mean_circ: 0.929527
Ran for 39550 frames Cell Events: 2486 mean_circ: 0.929301
Ran for 40050 frames Cell Events: 2526 mean_circ: 0.929519
Ran for 41550 frames Cell Events: 2633 mean_circ: 0.929493
Ran for 42550 frames Cell Events: 2697 mean_circ: 0.92975
Ran for 43050 frames Cell Events: 2735 mean_circ: 0.929808
Ran for 44050 frames Cell Events: 2796 mean_circ: 0.929559
Ran for 45550 frames Cell Events: 2898 mean_circ: 0.929363
Ran for 46050 frames Cell Events: 2922 mean_circ: 0.929614
Ran for 46550 frames Cell Events: 2941 mean_circ: 0.929633
Ran for 47050 frames Cell Events: 2975 mean_circ: 0.929615
Ran for 48050 frames Cell Events: 3050 mean_circ: 0.92964
Ran for 49050 frames Cell Events: 3120 mean_circ: 0.929954
Ran for 49550 frames Cell Events: 3148 mean_circ: 0.929965
Ran for 50050 frames Cell Events: 3178 mean_circ: 0.929965
Ran for 52050 frames Cell Events: 3312 mean_circ: 0.929619
Ran for 52550 frames Cell Events: 3346 mean_circ: 0.929796
Ran for 53050 frames Cell Events: 3368 mean_circ: 0.929806
Ran for 53550 frames Cell Events: 3395 mean_circ: 0.92978
Ran for 54050 frames Cell Events: 3413 mean_circ: 0.929779
Ran for 54550 frames Cell Events: 3447 mean_circ: 0.929695
Ran for 55550 frames Cell Events: 3518 mean_circ: 0.929636
Ran for 56050 frames Cell Events: 3548 mean_circ: 0.929596
Ran for 57050 frames Cell Events: 3590 mean_circ: 0.929634
Ran for 57550 frames Cell Events: 3615 mean_circ: 0.929481
Ran for 58050 frames Cell Events: 3635 mean_circ: 0.9295
Ran for 58550 frames Cell Events: 3656 mean_circ: 0.929486
Ran for 59050 frames Cell Events: 3691 mean_circ: 0.929463
Ran for 60550 frames Cell Events: 3790 mean_circ: 0.929258
Ran for 61050 frames Cell Events: 3810 mean_circ: 0.929186
Ran for 62050 frames Cell Events: 3874 mean_circ: 0.929259
Ran for 62550 frames Cell Events: 3899 mean_circ: 0.929289
Ran for 63050 frames Cell Events: 3927 mean_circ: 0.929297
Ran for 63550 frames Cell Events: 3948 mean_circ: 0.929239
Ran for 64050 frames Cell Events: 3980 mean_circ: 0.929297
Ran for 65550 frames Cell Events: 4066 mean_circ: 0.929271
Ran for 66550 frames Cell Events: 4123 mean_circ: 0.929266
Ran for 67050 frames Cell Events: 4146 mean_circ: 0.929237
Ran for 69050 frames Cell Events: 4280 mean_circ: 0.929184
Ran for 69550 frames Cell Events: 4309 mean_circ: 0.929294
Ran for 70050 frames Cell Events: 4339 mean_circ: 0.929118
Ran for 70550 frames Cell Events: 4364 mean_circ: 0.929074
Ran for 72050 frames Cell Events: 4474 mean_circ: 0.929143
Ran for 75050 frames Cell Events: 4691 mean_circ: 0.929391
Ran for 75550 frames Cell Events: 4711 mean_circ: 0.9294
Ran for 76050 frames Cell Events: 4746 mean_circ: 0.929361
Ran for 76550 frames Cell Events: 4768 mean_circ: 0.929472
Ran for 77050 frames Cell Events: 4783 mean_circ: 0.929454
Ran for 77550 frames Cell Events: 4825 mean_circ: 0.929543
Ran for 78550 frames Cell Events: 4892 mean_circ: 0.929544
Ran for 80050 frames Cell Events: 4984 mean_circ: 0.929382
Ran for 80550 frames Cell Events: 5020 mean_circ: 0.929368
Ran for 81550 frames Cell Events: 5105 mean_circ: 0.929368
Ran for 82550 frames Cell Events: 5184 mean_circ: 0.929535
Ran for 84050 frames Cell Events: 5262 mean_circ: 0.929477
Ran for 85050 frames Cell Events: 5323 mean_circ: 0.929496
Ran for 85550 frames Cell Events: 5346 mean_circ: 0.929457
Ran for 86050 frames Cell Events: 5379 mean_circ: 0.929444
Ran for 88050 frames Cell Events: 5510 mean_circ: 0.929329
Ran for 88550 frames Cell Events: 5540 mean_circ: 0.929333
Ran for 89050 frames Cell Events: 5567 mean_circ: 0.929403
Ran for 90050 frames Cell Events: 5658 mean_circ: 0.929366
Ran for 90550 frames Cell Events: 5696 mean_circ: 0.929426
Ran for 92050 frames Cell Events: 5783 mean_circ: 0.929415
Ran for 93050 frames Cell Events: 5834 mean_circ: 0.929497
Ran for 93550 frames Cell Events: 5864 mean_circ: 0.929504
Ran for 94550 frames Cell Events: 5905 mean_circ: 0.929503
Ran for 95550 frames Cell Events: 5960 mean_circ: 0.929519
Ran for 96050 frames Cell Events: 5983 mean_circ: 0.929548
Ran for 96550 frames Cell Events: 6011 mean_circ: 0.929516
Ran for 97050 frames Cell Events: 6053 mean_circ: 0.92953
Ran for 97550 frames Cell Events: 6076 mean_circ: 0.929506
Ran for 98050 frames Cell Events: 6101 mean_circ: 0.929491
Ran for 98550 frames Cell Events: 6134 mean_circ: 0.929527
Ran for 99050 frames Cell Events: 6148 mean_circ: 0.929539
Ran for 100050 frames Cell Events: 6197 mean_circ: 0.929537
Ran for 101050 frames Cell Events: 6249 mean_circ: 0.929582
Ran for 101550 frames Cell Events: 6279 mean_circ: 0.929562
Ran for 102050 frames Cell Events: 6306 mean_circ: 0.929602
Ran for 103050 frames Cell Events: 6369 mean_circ: 0.929556
Ran for 103550 frames Cell Events: 6400 mean_circ: 0.929562
Ran for 104550 frames Cell Events: 6476 mean_circ: 0.929581
Ran for 105550 frames Cell Events: 6527 mean_circ: 0.929607
Ran for 106050 frames Cell Events: 6552 mean_circ: 0.929606
Ran for 106550 frames Cell Events: 6573 mean_circ: 0.929624
Ran for 107550 frames Cell Events: 6637 mean_circ: 0.929605
Ran for 108550 frames Cell Events: 6703 mean_circ: 0.929468
Ran for 109550 frames Cell Events: 6762 mean_circ: 0.929479
Ran for 110050 frames Cell Events: 6784 mean_circ: 0.929514
Ran for 110550 frames Cell Events: 6805 mean_circ: 0.929544
Ran for 111050 frames Cell Events: 6837 mean_circ: 0.929524
Ran for 112550 frames Cell Events: 6935 mean_circ: 0.929414
Ran for 113050 frames Cell Events: 6965 mean_circ: 0.929402
Ran for 113550 frames Cell Events: 6991 mean_circ: 0.929386
Ran for 114050 frames Cell Events: 7016 mean_circ: 0.92941
Ran for 114550 frames Cell Events: 7049 mean_circ: 0.929327
Ran for 115050 frames Cell Events: 7076 mean_circ: 0.92932
Ran for 116050 frames Cell Events: 7128 mean_circ: 0.929224
Ran for 117550 frames Cell Events: 7220 mean_circ: 0.929282
Ran for 118050 frames Cell Events: 7234 mean_circ: 0.929277
Ran for 122550 frames Cell Events: 7556 mean_circ: 0.929138
Ran for 123050 frames Cell Events: 7580 mean_circ: 0.929127
Ran for 123550 frames Cell Events: 7629 mean_circ: 0.929069
Ran for 124550 frames Cell Events: 7699 mean_circ: 0.928977
Ran for 125550 frames Cell Events: 7763 mean_circ: 0.928987
Ran for 126550 frames Cell Events: 7815 mean_circ: 0.929001
Ran for 127550 frames Cell Events: 7869 mean_circ: 0.928931
Ran for 128050 frames Cell Events: 7901 mean_circ: 0.928862
Ran for 133050 frames Cell Events: 8208 mean_circ: 0.928764
Ran for 137550 frames Cell Events: 8538 mean_circ: 0.928919
Ran for 138550 frames Cell Events: 8617 mean_circ: 0.928817
Ran for 139050 frames Cell Events: 8649 mean_circ: 0.928803
Ran for 139550 frames Cell Events: 8682 mean_circ: 0.928743
Ran for 141050 frames Cell Events: 8790 mean_circ: 0.92881
Ran for 142050 frames Cell Events: 8869 mean_circ: 0.928857
Ran for 143050 frames Cell Events: 8921 mean_circ: 0.928934



End this measurment..



Stopping time: 3.69849e+008
****************************************
Measurement finished

Mean Circularitiy: 0.928932
Mean Area: 594.269
Mean FL-1 intensity: 3002.69
Mean FL-2 intensity: 4472.38
Mean FL-3 intensity: 0

frames processed: 141289
Total Time: 71602.5ms
Number of Runs (new bg img) 0
Frames collected: 143061
Skipped frames: 39490
Rescued Frames: 1883568
Samples DAQ: 35765351
Samples written: 71530620
Samples per Channel: 35765310
FPS: 1997.99

Samples per second: 499498
Expected samples/s: 500000

Events: 8921
Events per second: 124.591
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :218349
# 1 frames :55408
# 2 frames :2899
# 3 frames :1007
# 4 frames :423
# 5 frames :162
# 6 frames :2873
# 7 frames :985
# 8 frames :1126
# 9 frames :566
# 10 frames :362
# 11 frames :207
# 12 frames :245
# 13 frames :156
# 14 frames :297
# 15 frames :202
# 16 frames :133
# 17 frames :101
# 18 frames :80
# 19 frames :52
# 20 frames :59
# 21 frames :37
# 22 frames :42
# 23 frames :40
# 24 frames :33
# 25 frames :25
# 26 frames :24
# 27 frames :14
# 28 frames :16
# 29 frames :22
# 30 frames :18
# 31 frames :13
# 32 frames :13
# 33 frames :6
# 34 frames :8
# 35 frames :10
# 36 frames :9
# 37 frames :6
# 38 frames :6
# 39 frames :5
# 40 frames :4
# 41 frames :5
# 42 frames :2
# 43 frames :6
# 44 frames :4
# 45 frames :2
# 46 frames :4
# 47 frames :3
# 48 frames :1
# 49 frames :1
# 50 frames :3
# 51 frames :3
# 52 frames :2
# 53 frames :2
# 55 frames :6
# 68 frames :1
# 70 frames :1
# 73 frames :1
# 75 frames :1
# 79 frames :1
# 86 frames :1
# 93 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.4754
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0