Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.36.34 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bc98dd969adaa51961308b10d51d56c7",
"name": "M1_20170519_1556_dsRNA_L7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:37:09"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.2982
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.72824e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.942985
Ran for 1050 frames Cell Events: 38 mean_circ: 0.926229
Ran for 1550 frames Cell Events: 61 mean_circ: 0.921606
Ran for 2550 frames Cell Events: 112 mean_circ: 0.928448
Ran for 3050 frames Cell Events: 138 mean_circ: 0.929374
Ran for 4550 frames Cell Events: 209 mean_circ: 0.929039
Ran for 5050 frames Cell Events: 255 mean_circ: 0.929039
Ran for 5550 frames Cell Events: 277 mean_circ: 0.926428
Ran for 6550 frames Cell Events: 344 mean_circ: 0.927061
Ran for 8050 frames Cell Events: 411 mean_circ: 0.928188
Ran for 9050 frames Cell Events: 477 mean_circ: 0.929415
Ran for 9550 frames Cell Events: 510 mean_circ: 0.929756
Ran for 10050 frames Cell Events: 541 mean_circ: 0.929756
Ran for 11050 frames Cell Events: 594 mean_circ: 0.930821
Ran for 12550 frames Cell Events: 678 mean_circ: 0.931681
Ran for 13550 frames Cell Events: 738 mean_circ: 0.932581
Ran for 14050 frames Cell Events: 758 mean_circ: 0.932379
Ran for 15550 frames Cell Events: 822 mean_circ: 0.932387
Ran for 16550 frames Cell Events: 864 mean_circ: 0.931905
Ran for 17050 frames Cell Events: 886 mean_circ: 0.931738
Ran for 18550 frames Cell Events: 977 mean_circ: 0.931971
Ran for 20550 frames Cell Events: 1084 mean_circ: 0.932595
Ran for 21050 frames Cell Events: 1113 mean_circ: 0.932605
Ran for 21550 frames Cell Events: 1131 mean_circ: 0.932875
Ran for 23050 frames Cell Events: 1205 mean_circ: 0.933023
Ran for 23550 frames Cell Events: 1235 mean_circ: 0.932853
Ran for 24050 frames Cell Events: 1264 mean_circ: 0.932937
Ran for 25550 frames Cell Events: 1356 mean_circ: 0.932921
Ran for 27550 frames Cell Events: 1442 mean_circ: 0.932865
Ran for 29050 frames Cell Events: 1516 mean_circ: 0.932711
Ran for 31550 frames Cell Events: 1685 mean_circ: 0.93194
Ran for 32050 frames Cell Events: 1707 mean_circ: 0.932106
Ran for 32550 frames Cell Events: 1744 mean_circ: 0.932348
Ran for 33050 frames Cell Events: 1770 mean_circ: 0.932318
Ran for 33550 frames Cell Events: 1804 mean_circ: 0.932323
Ran for 34050 frames Cell Events: 1821 mean_circ: 0.932331
Ran for 35550 frames Cell Events: 1928 mean_circ: 0.932494
Ran for 36050 frames Cell Events: 1957 mean_circ: 0.93265
Ran for 36550 frames Cell Events: 1983 mean_circ: 0.932763
Ran for 37550 frames Cell Events: 2051 mean_circ: 0.932779
Ran for 38550 frames Cell Events: 2094 mean_circ: 0.932914
Ran for 40050 frames Cell Events: 2186 mean_circ: 0.932679
Ran for 40550 frames Cell Events: 2222 mean_circ: 0.932661
Ran for 42550 frames Cell Events: 2336 mean_circ: 0.932642
Ran for 45550 frames Cell Events: 2495 mean_circ: 0.932465
Ran for 46050 frames Cell Events: 2525 mean_circ: 0.932525
Ran for 47050 frames Cell Events: 2589 mean_circ: 0.932525
Ran for 48050 frames Cell Events: 2652 mean_circ: 0.932703
Ran for 49050 frames Cell Events: 2697 mean_circ: 0.932566
Ran for 49550 frames Cell Events: 2715 mean_circ: 0.93262
Ran for 50050 frames Cell Events: 2749 mean_circ: 0.932616
Ran for 50550 frames Cell Events: 2773 mean_circ: 0.932557
Ran for 51050 frames Cell Events: 2808 mean_circ: 0.932606
Ran for 51550 frames Cell Events: 2825 mean_circ: 0.932545
Ran for 52050 frames Cell Events: 2843 mean_circ: 0.932614
Ran for 52550 frames Cell Events: 2869 mean_circ: 0.93249
Ran for 53050 frames Cell Events: 2898 mean_circ: 0.932719
Ran for 53550 frames Cell Events: 2921 mean_circ: 0.932779
Ran for 56050 frames Cell Events: 3048 mean_circ: 0.932842
Ran for 57550 frames Cell Events: 3143 mean_circ: 0.93274
Ran for 58550 frames Cell Events: 3213 mean_circ: 0.932764
Ran for 59050 frames Cell Events: 3234 mean_circ: 0.932832
Ran for 59550 frames Cell Events: 3261 mean_circ: 0.932892
Ran for 61050 frames Cell Events: 3356 mean_circ: 0.93276
Ran for 61550 frames Cell Events: 3374 mean_circ: 0.932789
Ran for 62050 frames Cell Events: 3396 mean_circ: 0.932705
Ran for 63550 frames Cell Events: 3503 mean_circ: 0.932567
Ran for 64050 frames Cell Events: 3527 mean_circ: 0.932505
Ran for 65550 frames Cell Events: 3591 mean_circ: 0.932304
Ran for 66550 frames Cell Events: 3657 mean_circ: 0.932403
Ran for 67050 frames Cell Events: 3687 mean_circ: 0.932345
Ran for 67550 frames Cell Events: 3712 mean_circ: 0.932299
Ran for 68050 frames Cell Events: 3733 mean_circ: 0.932319
Ran for 68550 frames Cell Events: 3765 mean_circ: 0.932331
Ran for 70050 frames Cell Events: 3870 mean_circ: 0.932475
Ran for 72550 frames Cell Events: 4018 mean_circ: 0.932212
Ran for 73050 frames Cell Events: 4043 mean_circ: 0.932106
Ran for 74050 frames Cell Events: 4111 mean_circ: 0.932077
Ran for 74550 frames Cell Events: 4139 mean_circ: 0.932018
Ran for 75050 frames Cell Events: 4167 mean_circ: 0.931986
Ran for 75550 frames Cell Events: 4188 mean_circ: 0.931926
Ran for 77550 frames Cell Events: 4311 mean_circ: 0.93197
Ran for 78550 frames Cell Events: 4349 mean_circ: 0.931956
Ran for 80550 frames Cell Events: 4471 mean_circ: 0.931858
Ran for 81550 frames Cell Events: 4517 mean_circ: 0.931777
Ran for 82550 frames Cell Events: 4576 mean_circ: 0.93196
Ran for 83050 frames Cell Events: 4604 mean_circ: 0.931912
Ran for 84050 frames Cell Events: 4679 mean_circ: 0.931932
Ran for 84550 frames Cell Events: 4701 mean_circ: 0.931997
Ran for 86550 frames Cell Events: 4848 mean_circ: 0.932218
Ran for 87050 frames Cell Events: 4876 mean_circ: 0.932145
Ran for 88050 frames Cell Events: 4940 mean_circ: 0.932106
Ran for 88550 frames Cell Events: 4960 mean_circ: 0.932139
Ran for 89550 frames Cell Events: 5012 mean_circ: 0.932235
Ran for 91550 frames Cell Events: 5119 mean_circ: 0.932146
Ran for 93550 frames Cell Events: 5229 mean_circ: 0.932095
Ran for 94050 frames Cell Events: 5251 mean_circ: 0.932111
Ran for 96550 frames Cell Events: 5404 mean_circ: 0.932124
Ran for 97550 frames Cell Events: 5466 mean_circ: 0.932032
Ran for 98050 frames Cell Events: 5487 mean_circ: 0.932118
Ran for 98550 frames Cell Events: 5516 mean_circ: 0.932151
Ran for 99550 frames Cell Events: 5593 mean_circ: 0.932199
Ran for 101550 frames Cell Events: 5733 mean_circ: 0.932292
Ran for 102050 frames Cell Events: 5758 mean_circ: 0.932303
Ran for 102550 frames Cell Events: 5791 mean_circ: 0.932361
Ran for 103050 frames Cell Events: 5818 mean_circ: 0.932334
Ran for 104050 frames Cell Events: 5885 mean_circ: 0.932283
Ran for 105550 frames Cell Events: 5996 mean_circ: 0.932323
Ran for 107050 frames Cell Events: 6114 mean_circ: 0.932289
Ran for 107550 frames Cell Events: 6144 mean_circ: 0.932273
Ran for 108550 frames Cell Events: 6203 mean_circ: 0.932306
Ran for 110050 frames Cell Events: 6272 mean_circ: 0.932295
Ran for 110550 frames Cell Events: 6316 mean_circ: 0.932283
Ran for 111050 frames Cell Events: 6352 mean_circ: 0.932285
Ran for 112050 frames Cell Events: 6415 mean_circ: 0.932157
Ran for 113050 frames Cell Events: 6475 mean_circ: 0.932148
Ran for 114550 frames Cell Events: 6568 mean_circ: 0.932071
Ran for 115050 frames Cell Events: 6591 mean_circ: 0.932091
Ran for 116050 frames Cell Events: 6654 mean_circ: 0.931931
Ran for 118550 frames Cell Events: 6808 mean_circ: 0.931891
Ran for 120050 frames Cell Events: 6894 mean_circ: 0.931953
Ran for 121050 frames Cell Events: 6946 mean_circ: 0.93197
Ran for 121550 frames Cell Events: 6975 mean_circ: 0.931955
Ran for 122050 frames Cell Events: 7015 mean_circ: 0.931947
Ran for 123050 frames Cell Events: 7084 mean_circ: 0.93201
Ran for 123550 frames Cell Events: 7103 mean_circ: 0.932007
Ran for 124550 frames Cell Events: 7169 mean_circ: 0.932081
Ran for 125050 frames Cell Events: 7206 mean_circ: 0.932082
Ran for 125550 frames Cell Events: 7231 mean_circ: 0.932149
Ran for 126050 frames Cell Events: 7257 mean_circ: 0.932192
Ran for 128550 frames Cell Events: 7418 mean_circ: 0.932258
Ran for 129050 frames Cell Events: 7439 mean_circ: 0.932286
Ran for 130050 frames Cell Events: 7521 mean_circ: 0.932331
Ran for 130550 frames Cell Events: 7556 mean_circ: 0.932361
Ran for 133050 frames Cell Events: 7735 mean_circ: 0.932314
Ran for 133550 frames Cell Events: 7760 mean_circ: 0.932278
Ran for 134550 frames Cell Events: 7827 mean_circ: 0.932214
Ran for 135550 frames Cell Events: 7891 mean_circ: 0.932235
Ran for 138550 frames Cell Events: 8076 mean_circ: 0.932123
Ran for 139050 frames Cell Events: 8103 mean_circ: 0.932079
Ran for 140050 frames Cell Events: 8163 mean_circ: 0.932038
Ran for 140550 frames Cell Events: 8188 mean_circ: 0.932025
Ran for 141050 frames Cell Events: 8201 mean_circ: 0.931998
Ran for 142550 frames Cell Events: 8278 mean_circ: 0.931978
Ran for 143050 frames Cell Events: 8298 mean_circ: 0.932029
Ran for 144550 frames Cell Events: 8371 mean_circ: 0.932075
Ran for 145550 frames Cell Events: 8418 mean_circ: 0.932079
Ran for 146050 frames Cell Events: 8431 mean_circ: 0.932012
Ran for 146550 frames Cell Events: 8456 mean_circ: 0.932053
Ran for 147050 frames Cell Events: 8475 mean_circ: 0.932072
Ran for 148050 frames Cell Events: 8523 mean_circ: 0.932019
Ran for 148550 frames Cell Events: 8554 mean_circ: 0.932031
Ran for 149050 frames Cell Events: 8593 mean_circ: 0.932009
Ran for 150050 frames Cell Events: 8664 mean_circ: 0.93201
Ran for 155050 frames Cell Events: 8987 mean_circ: 0.931985
Ran for 155550 frames Cell Events: 9003 mean_circ: 0.931941
Ran for 156550 frames Cell Events: 9071 mean_circ: 0.931949
Ran for 157050 frames Cell Events: 9104 mean_circ: 0.931905
Ran for 157550 frames Cell Events: 9131 mean_circ: 0.931908
Ran for 158550 frames Cell Events: 9180 mean_circ: 0.931857
Ran for 159050 frames Cell Events: 9206 mean_circ: 0.931842
Ran for 160550 frames Cell Events: 9295 mean_circ: 0.931934
Ran for 161050 frames Cell Events: 9323 mean_circ: 0.931937
Ran for 161550 frames Cell Events: 9344 mean_circ: 0.93195
Ran for 163550 frames Cell Events: 9471 mean_circ: 0.93194
Ran for 164550 frames Cell Events: 9524 mean_circ: 0.931947
Ran for 165550 frames Cell Events: 9594 mean_circ: 0.931941
Ran for 166050 frames Cell Events: 9630 mean_circ: 0.931953
Ran for 167050 frames Cell Events: 9702 mean_circ: 0.931937
Ran for 168050 frames Cell Events: 9760 mean_circ: 0.931954
Ran for 168550 frames Cell Events: 9787 mean_circ: 0.931966
Ran for 169050 frames Cell Events: 9819 mean_circ: 0.9319
Ran for 170050 frames Cell Events: 9903 mean_circ: 0.931899
Ran for 171050 frames Cell Events: 9970 mean_circ: 0.931888



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 3.7291e+008
****************************************
Measurement finished

Mean Circularitiy: 0.931986
Mean Area: 606.127
Mean FL-1 intensity: 3293.07
Mean FL-2 intensity: 4448.52
Mean FL-3 intensity: 0

frames processed: 171111
Total Time: 85822.9ms
Number of Runs (new bg img) 0
Frames collected: 171472
Skipped frames: 54762
Rescued Frames: 2578416
Samples DAQ: 42869095
Samples written: 85735722
Samples per Channel: 42867861
FPS: 1997.98

Samples per second: 499492
Expected samples/s: 500000

Events: 10001
Events per second: 116.531
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :260072
# 1 frames :67892
# 2 frames :3464
# 3 frames :1222
# 4 frames :622
# 5 frames :235
# 6 frames :2595
# 7 frames :1595
# 8 frames :1427
# 9 frames :724
# 10 frames :451
# 11 frames :273
# 12 frames :292
# 13 frames :218
# 14 frames :310
# 15 frames :216
# 16 frames :172
# 17 frames :122
# 18 frames :99
# 19 frames :67
# 20 frames :75
# 21 frames :75
# 22 frames :72
# 23 frames :50
# 24 frames :56
# 25 frames :29
# 26 frames :36
# 27 frames :18
# 28 frames :22
# 29 frames :17
# 30 frames :10
# 31 frames :10
# 32 frames :16
# 33 frames :16
# 34 frames :7
# 35 frames :11
# 36 frames :5
# 37 frames :7
# 38 frames :6
# 39 frames :4
# 40 frames :2
# 41 frames :6
# 42 frames :8
# 43 frames :3
# 44 frames :2
# 45 frames :7
# 46 frames :2
# 47 frames :1
# 48 frames :2
# 49 frames :1
# 51 frames :1
# 53 frames :1
# 56 frames :1
# 61 frames :1
# 68 frames :1
# 72 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.2982
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0