Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.40.23 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "03befaf63a2d384e8f2402975bc870e4",
"name": "M1_20170519_1632_dsRNA_L8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:40:58"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.4749
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.74991e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 40 mean_circ: 0.893359
Ran for 2050 frames Cell Events: 90 mean_circ: 0.898821
Ran for 2550 frames Cell Events: 102 mean_circ: 0.900351
Ran for 3050 frames Cell Events: 130 mean_circ: 0.899534
Ran for 4550 frames Cell Events: 167 mean_circ: 0.898441
Ran for 5050 frames Cell Events: 192 mean_circ: 0.898903
Ran for 7050 frames Cell Events: 291 mean_circ: 0.900039
Ran for 7550 frames Cell Events: 318 mean_circ: 0.900283
Ran for 8050 frames Cell Events: 344 mean_circ: 0.898084
Ran for 9550 frames Cell Events: 413 mean_circ: 0.898115
Ran for 10050 frames Cell Events: 426 mean_circ: 0.897149
Ran for 11050 frames Cell Events: 469 mean_circ: 0.897809
Ran for 11550 frames Cell Events: 474 mean_circ: 0.898108
Ran for 12550 frames Cell Events: 524 mean_circ: 0.89798
Ran for 13050 frames Cell Events: 538 mean_circ: 0.898413
Ran for 13550 frames Cell Events: 560 mean_circ: 0.89851
Ran for 14050 frames Cell Events: 571 mean_circ: 0.898125
Ran for 15050 frames Cell Events: 630 mean_circ: 0.898402
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Ran for 16550 frames Cell Events: 694 mean_circ: 0.898288
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Ran for 64550 frames Cell Events: 2768 mean_circ: 0.897006
Ran for 65050 frames Cell Events: 2794 mean_circ: 0.897154
Ran for 65550 frames Cell Events: 2813 mean_circ: 0.89691
Ran for 67050 frames Cell Events: 2868 mean_circ: 0.896881
Ran for 70050 frames Cell Events: 3009 mean_circ: 0.896397
Ran for 70550 frames Cell Events: 3034 mean_circ: 0.896422
Ran for 71050 frames Cell Events: 3053 mean_circ: 0.896402
Ran for 72050 frames Cell Events: 3097 mean_circ: 0.896474
Ran for 72550 frames Cell Events: 3115 mean_circ: 0.896438
Ran for 73550 frames Cell Events: 3163 mean_circ: 0.89652
Ran for 74050 frames Cell Events: 3193 mean_circ: 0.896614
Ran for 75050 frames Cell Events: 3259 mean_circ: 0.897104
Ran for 75550 frames Cell Events: 3282 mean_circ: 0.896938
Ran for 76050 frames Cell Events: 3313 mean_circ: 0.89698
Ran for 80050 frames Cell Events: 3538 mean_circ: 0.896959
Ran for 80550 frames Cell Events: 3554 mean_circ: 0.897037
Ran for 81050 frames Cell Events: 3585 mean_circ: 0.897037
Ran for 82050 frames Cell Events: 3637 mean_circ: 0.896968
Ran for 82550 frames Cell Events: 3655 mean_circ: 0.89699
Ran for 83050 frames Cell Events: 3669 mean_circ: 0.896938
Ran for 84550 frames Cell Events: 3740 mean_circ: 0.896814
Ran for 85050 frames Cell Events: 3764 mean_circ: 0.89672
Ran for 85550 frames Cell Events: 3782 mean_circ: 0.896711
Ran for 86050 frames Cell Events: 3802 mean_circ: 0.896708
Ran for 86550 frames Cell Events: 3822 mean_circ: 0.896755
Ran for 87050 frames Cell Events: 3846 mean_circ: 0.896715
Ran for 87550 frames Cell Events: 3872 mean_circ: 0.896843
Ran for 88050 frames Cell Events: 3887 mean_circ: 0.896837
Ran for 89050 frames Cell Events: 3927 mean_circ: 0.89686
Ran for 90550 frames Cell Events: 3977 mean_circ: 0.897048
Ran for 91050 frames Cell Events: 3999 mean_circ: 0.897086
Ran for 92050 frames Cell Events: 4044 mean_circ: 0.897148
Ran for 93050 frames Cell Events: 4108 mean_circ: 0.897133
Ran for 96050 frames Cell Events: 4282 mean_circ: 0.896944
Ran for 96550 frames Cell Events: 4306 mean_circ: 0.896851
Ran for 97050 frames Cell Events: 4330 mean_circ: 0.896869
Ran for 97550 frames Cell Events: 4346 mean_circ: 0.896862
Ran for 98550 frames Cell Events: 4400 mean_circ: 0.897002
Ran for 99050 frames Cell Events: 4422 mean_circ: 0.89694
Ran for 99550 frames Cell Events: 4447 mean_circ: 0.896925
Ran for 100050 frames Cell Events: 4467 mean_circ: 0.896892
Ran for 100550 frames Cell Events: 4493 mean_circ: 0.896892
Ran for 101050 frames Cell Events: 4510 mean_circ: 0.896917
Ran for 102050 frames Cell Events: 4553 mean_circ: 0.89696
Ran for 102550 frames Cell Events: 4572 mean_circ: 0.896899
Ran for 103050 frames Cell Events: 4595 mean_circ: 0.89682
Ran for 104050 frames Cell Events: 4632 mean_circ: 0.896884
Ran for 106050 frames Cell Events: 4721 mean_circ: 0.896652
Ran for 107050 frames Cell Events: 4771 mean_circ: 0.896578
Ran for 107550 frames Cell Events: 4807 mean_circ: 0.89662
Ran for 108050 frames Cell Events: 4835 mean_circ: 0.8965
Ran for 108550 frames Cell Events: 4871 mean_circ: 0.896505
Ran for 109550 frames Cell Events: 4928 mean_circ: 0.896397
Ran for 111550 frames Cell Events: 4997 mean_circ: 0.896478
Ran for 112050 frames Cell Events: 5022 mean_circ: 0.896419
Ran for 113050 frames Cell Events: 5069 mean_circ: 0.896378
Ran for 113550 frames Cell Events: 5090 mean_circ: 0.896265
Ran for 114050 frames Cell Events: 5115 mean_circ: 0.896269
Ran for 114550 frames Cell Events: 5133 mean_circ: 0.896284
Ran for 115050 frames Cell Events: 5153 mean_circ: 0.896303
Ran for 117050 frames Cell Events: 5254 mean_circ: 0.896131
Ran for 119050 frames Cell Events: 5344 mean_circ: 0.896081
Ran for 119550 frames Cell Events: 5368 mean_circ: 0.895994
Ran for 120050 frames Cell Events: 5383 mean_circ: 0.896029
Ran for 122050 frames Cell Events: 5466 mean_circ: 0.895986
Ran for 122550 frames Cell Events: 5487 mean_circ: 0.896078
Ran for 123550 frames Cell Events: 5530 mean_circ: 0.896122
Ran for 124050 frames Cell Events: 5550 mean_circ: 0.896083
Ran for 125050 frames Cell Events: 5564 mean_circ: 0.896063
Ran for 125550 frames Cell Events: 5579 mean_circ: 0.896026
Ran for 126050 frames Cell Events: 5597 mean_circ: 0.896016
Ran for 126550 frames Cell Events: 5612 mean_circ: 0.896027
Ran for 128050 frames Cell Events: 5668 mean_circ: 0.895998
Ran for 128550 frames Cell Events: 5682 mean_circ: 0.895878
Ran for 129050 frames Cell Events: 5704 mean_circ: 0.895863
Ran for 130050 frames Cell Events: 5739 mean_circ: 0.895824
Ran for 130550 frames Cell Events: 5757 mean_circ: 0.895793
Ran for 132550 frames Cell Events: 5861 mean_circ: 0.895697
Ran for 133050 frames Cell Events: 5880 mean_circ: 0.895732
Ran for 133550 frames Cell Events: 5900 mean_circ: 0.895743
Ran for 134550 frames Cell Events: 5958 mean_circ: 0.895778
Ran for 135050 frames Cell Events: 5986 mean_circ: 0.89569
Ran for 136050 frames Cell Events: 6010 mean_circ: 0.895554
Ran for 137550 frames Cell Events: 6091 mean_circ: 0.895517
Ran for 138050 frames Cell Events: 6111 mean_circ: 0.895533
Ran for 139050 frames Cell Events: 6148 mean_circ: 0.895477
Ran for 139550 frames Cell Events: 6180 mean_circ: 0.895466
Ran for 140550 frames Cell Events: 6228 mean_circ: 0.895662
Ran for 141550 frames Cell Events: 6283 mean_circ: 0.895674
Ran for 144050 frames Cell Events: 6436 mean_circ: 0.895527
Ran for 144550 frames Cell Events: 6471 mean_circ: 0.89558
Ran for 145550 frames Cell Events: 6521 mean_circ: 0.8955
Ran for 146050 frames Cell Events: 6540 mean_circ: 0.895509
Ran for 146550 frames Cell Events: 6560 mean_circ: 0.895532
Ran for 147050 frames Cell Events: 6581 mean_circ: 0.895532
Ran for 147550 frames Cell Events: 6604 mean_circ: 0.895502
Ran for 149050 frames Cell Events: 6658 mean_circ: 0.895436
Ran for 149550 frames Cell Events: 6684 mean_circ: 0.895453
Ran for 150050 frames Cell Events: 6706 mean_circ: 0.895443
Ran for 150550 frames Cell Events: 6727 mean_circ: 0.895492
Ran for 151550 frames Cell Events: 6778 mean_circ: 0.895504
Ran for 152550 frames Cell Events: 6827 mean_circ: 0.895455
Ran for 153050 frames Cell Events: 6852 mean_circ: 0.89548
Ran for 153550 frames Cell Events: 6871 mean_circ: 0.895474
Ran for 154550 frames Cell Events: 6925 mean_circ: 0.89545
Ran for 156050 frames Cell Events: 7001 mean_circ: 0.895348
Ran for 156550 frames Cell Events: 7012 mean_circ: 0.895374
Ran for 157050 frames Cell Events: 7036 mean_circ: 0.895372
Ran for 157550 frames Cell Events: 7064 mean_circ: 0.895394
Ran for 158050 frames Cell Events: 7083 mean_circ: 0.895376
Ran for 158550 frames Cell Events: 7107 mean_circ: 0.895443
Ran for 159050 frames Cell Events: 7128 mean_circ: 0.895486
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Ran for 162550 frames Cell Events: 7288 mean_circ: 0.89569
Ran for 163550 frames Cell Events: 7337 mean_circ: 0.895636
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Ran for 172050 frames Cell Events: 7738 mean_circ: 0.895392
Ran for 173050 frames Cell Events: 7799 mean_circ: 0.895333
Ran for 173550 frames Cell Events: 7820 mean_circ: 0.895404
Ran for 174050 frames Cell Events: 7831 mean_circ: 0.895414
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Ran for 193050 frames Cell Events: 8712 mean_circ: 0.895439
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Ran for 200050 frames Cell Events: 9051 mean_circ: 0.895201
Ran for 201050 frames Cell Events: 9087 mean_circ: 0.895214
Ran for 201550 frames Cell Events: 9098 mean_circ: 0.895242
Ran for 202050 frames Cell Events: 9113 mean_circ: 0.895226
Ran for 202550 frames Cell Events: 9142 mean_circ: 0.895245
Ran for 203050 frames Cell Events: 9154 mean_circ: 0.895239
Ran for 203550 frames Cell Events: 9176 mean_circ: 0.895199
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Ran for 206050 frames Cell Events: 9295 mean_circ: 0.895005
Ran for 207050 frames Cell Events: 9339 mean_circ: 0.894904
Ran for 208050 frames Cell Events: 9390 mean_circ: 0.894844
Ran for 208550 frames Cell Events: 9406 mean_circ: 0.894867
Ran for 210050 frames Cell Events: 9474 mean_circ: 0.894788
Ran for 211050 frames Cell Events: 9517 mean_circ: 0.894784
Ran for 212050 frames Cell Events: 9568 mean_circ: 0.894745
Ran for 212550 frames Cell Events: 9590 mean_circ: 0.894706
Ran for 213050 frames Cell Events: 9612 mean_circ: 0.8947
Ran for 214050 frames Cell Events: 9664 mean_circ: 0.894681
Ran for 215050 frames Cell Events: 9702 mean_circ: 0.894595
Ran for 216050 frames Cell Events: 9737 mean_circ: 0.894598
Ran for 216550 frames Cell Events: 9761 mean_circ: 0.894624
Ran for 217550 frames Cell Events: 9811 mean_circ: 0.894646
Ran for 218050 frames Cell Events: 9840 mean_circ: 0.894644
Ran for 219550 frames Cell Events: 9928 mean_circ: 0.894552
Ran for 220050 frames Cell Events: 9942 mean_circ: 0.894591
Ran for 220550 frames Cell Events: 9959 mean_circ: 0.894605
Ran for 221050 frames Cell Events: 9980 mean_circ: 0.894622



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 3.75102e+008
****************************************
Measurement finished

Mean Circularitiy: 0.894585
Mean Area: 620.184
Mean FL-1 intensity: 2323.36
Mean FL-2 intensity: 3523.88
Mean FL-3 intensity: 0

frames processed: 217631
Total Time: 110760ms
Number of Runs (new bg img) 0
Frames collected: 221395
Skipped frames: 64964
Rescued Frames: 3118870
Samples DAQ: 55351751
Samples written: 110697516
Samples per Channel: 55348758
FPS: 1998.87

Samples per second: 499717
Expected samples/s: 500000

Events: 10001
Events per second: 90.2942
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :320113
# 1 frames :105262
# 2 frames :4808
# 3 frames :1402
# 4 frames :776
# 5 frames :282
# 6 frames :2380
# 7 frames :2482
# 8 frames :1422
# 9 frames :940
# 10 frames :537
# 11 frames :320
# 12 frames :299
# 13 frames :291
# 14 frames :251
# 15 frames :228
# 16 frames :181
# 17 frames :136
# 18 frames :91
# 19 frames :82
# 20 frames :70
# 21 frames :56
# 22 frames :45
# 23 frames :38
# 24 frames :39
# 25 frames :27
# 26 frames :14
# 27 frames :27
# 28 frames :17
# 29 frames :10
# 30 frames :9
# 31 frames :10
# 32 frames :17
# 33 frames :7
# 34 frames :3
# 35 frames :4
# 36 frames :4
# 37 frames :7
# 38 frames :5
# 39 frames :5
# 40 frames :7
# 41 frames :2
# 45 frames :1
# 46 frames :1
# 48 frames :1
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 53 frames :1
# 54 frames :1
# 57 frames :1
# 58 frames :2
# 61 frames :1
# 70 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.4749
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0