Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.51.33 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "87b8673fe51a1e896eb9146b3b85ac60",
"name": "M1_20170521_1530_dsRNA_B4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:52:00"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8535 (max 8536) in '8535'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6198
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.44078e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 14 mean_circ: 0.945016
Ran for 2550 frames Cell Events: 95 mean_circ: 0.931837
Ran for 3550 frames Cell Events: 112 mean_circ: 0.930913
Ran for 4050 frames Cell Events: 133 mean_circ: 0.931564
Ran for 5550 frames Cell Events: 205 mean_circ: 0.934059
Ran for 6050 frames Cell Events: 227 mean_circ: 0.933706
Ran for 6550 frames Cell Events: 242 mean_circ: 0.933495
Ran for 7050 frames Cell Events: 255 mean_circ: 0.93322
Ran for 8050 frames Cell Events: 302 mean_circ: 0.933487
Ran for 8550 frames Cell Events: 326 mean_circ: 0.933617
Ran for 9550 frames Cell Events: 375 mean_circ: 0.933844
Ran for 10050 frames Cell Events: 390 mean_circ: 0.932751
Ran for 10550 frames Cell Events: 403 mean_circ: 0.932792
Ran for 12050 frames Cell Events: 473 mean_circ: 0.931636
Ran for 13050 frames Cell Events: 503 mean_circ: 0.931266
Ran for 13550 frames Cell Events: 517 mean_circ: 0.931487
Ran for 14050 frames Cell Events: 530 mean_circ: 0.931154
Ran for 14550 frames Cell Events: 547 mean_circ: 0.930949
Ran for 15050 frames Cell Events: 567 mean_circ: 0.930736
Ran for 15550 frames Cell Events: 580 mean_circ: 0.930602
Ran for 16050 frames Cell Events: 607 mean_circ: 0.93054
Ran for 17050 frames Cell Events: 647 mean_circ: 0.930261
Ran for 19050 frames Cell Events: 703 mean_circ: 0.930419
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Ran for 63550 frames Cell Events: 2315 mean_circ: 0.931195
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Ran for 68550 frames Cell Events: 2495 mean_circ: 0.931442
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Ran for 74050 frames Cell Events: 2692 mean_circ: 0.93156
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Ran for 84050 frames Cell Events: 3122 mean_circ: 0.931755
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Ran for 86050 frames Cell Events: 3200 mean_circ: 0.931701
Ran for 87050 frames Cell Events: 3235 mean_circ: 0.931757
Ran for 87550 frames Cell Events: 3254 mean_circ: 0.931828
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Ran for 90050 frames Cell Events: 3353 mean_circ: 0.931992
Ran for 90550 frames Cell Events: 3368 mean_circ: 0.931948
Ran for 91550 frames Cell Events: 3410 mean_circ: 0.931781
Ran for 92050 frames Cell Events: 3422 mean_circ: 0.93174
Ran for 92550 frames Cell Events: 3445 mean_circ: 0.931754
Ran for 93050 frames Cell Events: 3464 mean_circ: 0.931727
Ran for 93550 frames Cell Events: 3483 mean_circ: 0.931574
Ran for 94050 frames Cell Events: 3503 mean_circ: 0.931607
Ran for 95550 frames Cell Events: 3560 mean_circ: 0.931663
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Ran for 96550 frames Cell Events: 3601 mean_circ: 0.931709
Ran for 97050 frames Cell Events: 3622 mean_circ: 0.931711
Ran for 98550 frames Cell Events: 3658 mean_circ: 0.931696
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Ran for 99550 frames Cell Events: 3716 mean_circ: 0.931812
Ran for 100050 frames Cell Events: 3734 mean_circ: 0.931792
Ran for 100550 frames Cell Events: 3759 mean_circ: 0.931806
Ran for 101050 frames Cell Events: 3775 mean_circ: 0.931833
Ran for 102050 frames Cell Events: 3816 mean_circ: 0.931794
Ran for 103050 frames Cell Events: 3869 mean_circ: 0.931616
Ran for 105050 frames Cell Events: 3938 mean_circ: 0.931729
Ran for 106050 frames Cell Events: 3977 mean_circ: 0.931669
Ran for 107550 frames Cell Events: 4071 mean_circ: 0.931696
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Ran for 120550 frames Cell Events: 4662 mean_circ: 0.931437
Ran for 121050 frames Cell Events: 4684 mean_circ: 0.931412
Ran for 121550 frames Cell Events: 4704 mean_circ: 0.931459
Ran for 122050 frames Cell Events: 4724 mean_circ: 0.931476
Ran for 122550 frames Cell Events: 4736 mean_circ: 0.93142
Ran for 123050 frames Cell Events: 4755 mean_circ: 0.931449
Ran for 123550 frames Cell Events: 4774 mean_circ: 0.931406
Ran for 124050 frames Cell Events: 4801 mean_circ: 0.931445
Ran for 124550 frames Cell Events: 4819 mean_circ: 0.931435
Ran for 125050 frames Cell Events: 4840 mean_circ: 0.931417
Ran for 125550 frames Cell Events: 4858 mean_circ: 0.931406
Ran for 126050 frames Cell Events: 4873 mean_circ: 0.931345
Ran for 126550 frames Cell Events: 4889 mean_circ: 0.931379
Ran for 127050 frames Cell Events: 4911 mean_circ: 0.931347
Ran for 127550 frames Cell Events: 4931 mean_circ: 0.93136
Ran for 128050 frames Cell Events: 4947 mean_circ: 0.931417
Ran for 128550 frames Cell Events: 4973 mean_circ: 0.931407
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Ran for 132550 frames Cell Events: 5114 mean_circ: 0.931368
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Ran for 144050 frames Cell Events: 5550 mean_circ: 0.93109
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Ran for 147050 frames Cell Events: 5678 mean_circ: 0.9311
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Ran for 172550 frames Cell Events: 6580 mean_circ: 0.930952
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Ran for 173550 frames Cell Events: 6619 mean_circ: 0.930871
Ran for 174550 frames Cell Events: 6659 mean_circ: 0.930967
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Ran for 180550 frames Cell Events: 6874 mean_circ: 0.930933
Ran for 182050 frames Cell Events: 6933 mean_circ: 0.93092
Ran for 182550 frames Cell Events: 6950 mean_circ: 0.930898
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Ran for 195050 frames Cell Events: 7478 mean_circ: 0.930929
Ran for 195550 frames Cell Events: 7491 mean_circ: 0.930914
Ran for 196050 frames Cell Events: 7514 mean_circ: 0.930927
Ran for 196550 frames Cell Events: 7540 mean_circ: 0.930916
Ran for 197550 frames Cell Events: 7585 mean_circ: 0.930943
Ran for 198550 frames Cell Events: 7625 mean_circ: 0.930968
Ran for 199050 frames Cell Events: 7644 mean_circ: 0.930964
Ran for 199550 frames Cell Events: 7666 mean_circ: 0.93096
Ran for 201050 frames Cell Events: 7732 mean_circ: 0.93102
Ran for 202050 frames Cell Events: 7781 mean_circ: 0.930947
Ran for 202550 frames Cell Events: 7799 mean_circ: 0.930962
Ran for 203050 frames Cell Events: 7811 mean_circ: 0.930908
Ran for 203550 frames Cell Events: 7838 mean_circ: 0.930803
Ran for 204050 frames Cell Events: 7858 mean_circ: 0.930757
Ran for 205050 frames Cell Events: 7893 mean_circ: 0.930731
Ran for 205550 frames Cell Events: 7914 mean_circ: 0.930727
Ran for 206550 frames Cell Events: 7955 mean_circ: 0.93077
Ran for 207050 frames Cell Events: 7971 mean_circ: 0.930791
Ran for 207550 frames Cell Events: 7987 mean_circ: 0.930839
Ran for 208050 frames Cell Events: 8005 mean_circ: 0.930833
Ran for 208550 frames Cell Events: 8029 mean_circ: 0.930858
Ran for 209550 frames Cell Events: 8072 mean_circ: 0.930787
Ran for 210050 frames Cell Events: 8091 mean_circ: 0.930798
Ran for 210550 frames Cell Events: 8105 mean_circ: 0.930808
Ran for 211050 frames Cell Events: 8125 mean_circ: 0.930833
Ran for 211550 frames Cell Events: 8153 mean_circ: 0.930851
Ran for 212050 frames Cell Events: 8167 mean_circ: 0.930853
Ran for 213550 frames Cell Events: 8238 mean_circ: 0.930803
Ran for 214050 frames Cell Events: 8255 mean_circ: 0.930757
Ran for 215050 frames Cell Events: 8297 mean_circ: 0.930769
Ran for 215550 frames Cell Events: 8309 mean_circ: 0.930764
Ran for 216050 frames Cell Events: 8330 mean_circ: 0.93076
Ran for 216550 frames Cell Events: 8361 mean_circ: 0.930737
Ran for 217550 frames Cell Events: 8394 mean_circ: 0.930741
Ran for 218050 frames Cell Events: 8419 mean_circ: 0.93074
Ran for 218550 frames Cell Events: 8436 mean_circ: 0.930767
Ran for 219050 frames Cell Events: 8454 mean_circ: 0.930709
Ran for 219550 frames Cell Events: 8478 mean_circ: 0.930719



End this measurment..



Stopping time: 5.44188e+008
****************************************
Measurement finished

Mean Circularitiy: 0.930706
Mean Area: 589.938
Mean FL-1 intensity: 3024.48
Mean FL-2 intensity: 4161.78
Mean FL-3 intensity: 0

frames processed: 218643
Total Time: 110496ms
Number of Runs (new bg img) 0
Frames collected: 220876
Skipped frames: 18572
Rescued Frames: 792125
Samples DAQ: 55220068
Samples written: 110437780
Samples per Channel: 55218890
FPS: 1998.95

Samples per second: 499736
Expected samples/s: 500000

Events: 8536
Events per second: 77.2516
jumped back (PMT): 7
#Frames read, frequency
# 0 frames :301446
# 1 frames :124479
# 2 frames :4380
# 3 frames :1389
# 4 frames :519
# 5 frames :198
# 6 frames :3834
# 7 frames :1549
# 8 frames :1291
# 9 frames :518
# 10 frames :444
# 11 frames :350
# 12 frames :332
# 13 frames :161
# 14 frames :211
# 15 frames :123
# 16 frames :117
# 17 frames :71
# 18 frames :34
# 19 frames :44
# 20 frames :45
# 21 frames :35
# 22 frames :33
# 23 frames :19
# 24 frames :11
# 25 frames :8
# 26 frames :15
# 27 frames :10
# 28 frames :7
# 29 frames :4
# 30 frames :6
# 31 frames :6
# 32 frames :1
# 33 frames :4
# 34 frames :4
# 35 frames :5
# 36 frames :3
# 37 frames :1
# 38 frames :4
# 39 frames :1
# 40 frames :1
# 41 frames :1
# 49 frames :1
# 50 frames :2
# 53 frames :1
# 56 frames :1
# 57 frames :1
# 63 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6198
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0