Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.52.46 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3080c38648a829ecdc096ec7487dfc33",
"name": "M1_20170521_1542_dsRNA_B8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:53:13"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8084 (max 8085) in '8084'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7262
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.4476e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 60 mean_circ: 0.933242
Ran for 2050 frames Cell Events: 79 mean_circ: 0.934413
Ran for 2550 frames Cell Events: 96 mean_circ: 0.934732
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Ran for 3550 frames Cell Events: 129 mean_circ: 0.935687
Ran for 4050 frames Cell Events: 144 mean_circ: 0.936326
Ran for 5050 frames Cell Events: 197 mean_circ: 0.935771
Ran for 5550 frames Cell Events: 226 mean_circ: 0.934425
Ran for 6050 frames Cell Events: 247 mean_circ: 0.935909
Ran for 8050 frames Cell Events: 333 mean_circ: 0.935751
Ran for 11050 frames Cell Events: 412 mean_circ: 0.936141
Ran for 11550 frames Cell Events: 431 mean_circ: 0.936166
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Ran for 12550 frames Cell Events: 483 mean_circ: 0.9343
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Ran for 15050 frames Cell Events: 565 mean_circ: 0.934763
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Ran for 17050 frames Cell Events: 647 mean_circ: 0.933169
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Ran for 67050 frames Cell Events: 2442 mean_circ: 0.930169
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Ran for 72050 frames Cell Events: 2614 mean_circ: 0.929778
Ran for 74550 frames Cell Events: 2711 mean_circ: 0.929672
Ran for 75550 frames Cell Events: 2745 mean_circ: 0.929617
Ran for 76550 frames Cell Events: 2781 mean_circ: 0.929554
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Ran for 91550 frames Cell Events: 3349 mean_circ: 0.929134
Ran for 92550 frames Cell Events: 3383 mean_circ: 0.929125
Ran for 94550 frames Cell Events: 3460 mean_circ: 0.929429
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Ran for 96050 frames Cell Events: 3509 mean_circ: 0.929475
Ran for 97550 frames Cell Events: 3567 mean_circ: 0.929629
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Ran for 100550 frames Cell Events: 3667 mean_circ: 0.929591
Ran for 101050 frames Cell Events: 3683 mean_circ: 0.929621
Ran for 101550 frames Cell Events: 3698 mean_circ: 0.929571
Ran for 102050 frames Cell Events: 3711 mean_circ: 0.929539
Ran for 103050 frames Cell Events: 3745 mean_circ: 0.92957
Ran for 103550 frames Cell Events: 3762 mean_circ: 0.929682
Ran for 104050 frames Cell Events: 3783 mean_circ: 0.929679
Ran for 104550 frames Cell Events: 3794 mean_circ: 0.929674
Ran for 105550 frames Cell Events: 3823 mean_circ: 0.929659
Ran for 106050 frames Cell Events: 3847 mean_circ: 0.929774
Ran for 106550 frames Cell Events: 3864 mean_circ: 0.929688
Ran for 107550 frames Cell Events: 3895 mean_circ: 0.929639
Ran for 108550 frames Cell Events: 3927 mean_circ: 0.929723
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Ran for 121550 frames Cell Events: 4441 mean_circ: 0.929356
Ran for 122050 frames Cell Events: 4452 mean_circ: 0.92935
Ran for 123050 frames Cell Events: 4484 mean_circ: 0.929318
Ran for 124050 frames Cell Events: 4521 mean_circ: 0.92937
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Ran for 125050 frames Cell Events: 4555 mean_circ: 0.929392
Ran for 125550 frames Cell Events: 4575 mean_circ: 0.929424
Ran for 126550 frames Cell Events: 4617 mean_circ: 0.929438
Ran for 127050 frames Cell Events: 4629 mean_circ: 0.929484
Ran for 127550 frames Cell Events: 4649 mean_circ: 0.929497
Ran for 128050 frames Cell Events: 4678 mean_circ: 0.929448
Ran for 128550 frames Cell Events: 4691 mean_circ: 0.929537
Ran for 129050 frames Cell Events: 4717 mean_circ: 0.92957
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Ran for 130550 frames Cell Events: 4763 mean_circ: 0.929572
Ran for 131550 frames Cell Events: 4795 mean_circ: 0.929621
Ran for 134050 frames Cell Events: 4921 mean_circ: 0.92973
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Ran for 142550 frames Cell Events: 5328 mean_circ: 0.92978
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Ran for 143550 frames Cell Events: 5374 mean_circ: 0.929803
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Ran for 145550 frames Cell Events: 5454 mean_circ: 0.929834
Ran for 146050 frames Cell Events: 5473 mean_circ: 0.929837
Ran for 147550 frames Cell Events: 5541 mean_circ: 0.929812
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Ran for 154050 frames Cell Events: 5768 mean_circ: 0.929865
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Ran for 156550 frames Cell Events: 5869 mean_circ: 0.930008
Ran for 157050 frames Cell Events: 5884 mean_circ: 0.930009
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Ran for 203050 frames Cell Events: 7714 mean_circ: 0.930259
Ran for 203550 frames Cell Events: 7740 mean_circ: 0.930265
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Ran for 204550 frames Cell Events: 7769 mean_circ: 0.930255
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Ran for 205550 frames Cell Events: 7812 mean_circ: 0.930321
Ran for 206550 frames Cell Events: 7856 mean_circ: 0.930346
Ran for 207050 frames Cell Events: 7880 mean_circ: 0.930372
Ran for 207550 frames Cell Events: 7912 mean_circ: 0.93037
Ran for 208050 frames Cell Events: 7927 mean_circ: 0.930423
Ran for 208550 frames Cell Events: 7945 mean_circ: 0.930441
Ran for 209050 frames Cell Events: 7957 mean_circ: 0.930471
Ran for 210050 frames Cell Events: 7998 mean_circ: 0.930497
Ran for 211050 frames Cell Events: 8043 mean_circ: 0.930462



End this measurment..



Stopping time: 5.44866e+008
****************************************
Measurement finished

Mean Circularitiy: 0.930457
Mean Area: 640.11
Mean FL-1 intensity: 3142.57
Mean FL-2 intensity: 4227.32
Mean FL-3 intensity: 0

frames processed: 209794
Total Time: 106191ms
Number of Runs (new bg img) 0
Frames collected: 212250
Skipped frames: 21989
Rescued Frames: 972139
Samples DAQ: 53062853
Samples written: 106124820
Samples per Channel: 53062410
FPS: 1998.76

Samples per second: 499689
Expected samples/s: 500000

Events: 8085
Events per second: 76.1365
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :304098
# 1 frames :104629
# 2 frames :4030
# 3 frames :1613
# 4 frames :817
# 5 frames :399
# 6 frames :2138
# 7 frames :1229
# 8 frames :1456
# 9 frames :969
# 10 frames :661
# 11 frames :424
# 12 frames :393
# 13 frames :251
# 14 frames :231
# 15 frames :200
# 16 frames :152
# 17 frames :142
# 18 frames :106
# 19 frames :90
# 20 frames :79
# 21 frames :59
# 22 frames :45
# 23 frames :39
# 24 frames :35
# 25 frames :22
# 26 frames :18
# 27 frames :22
# 28 frames :15
# 29 frames :17
# 30 frames :10
# 31 frames :12
# 32 frames :9
# 33 frames :19
# 34 frames :3
# 35 frames :7
# 36 frames :3
# 37 frames :3
# 38 frames :2
# 39 frames :8
# 40 frames :3
# 41 frames :4
# 42 frames :1
# 43 frames :2
# 44 frames :2
# 45 frames :2
# 50 frames :1
# 53 frames :1
# 58 frames :1
# 69 frames :1
# 72 frames :2
# 82 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7262
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0