Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.04.41 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7d1e602b2cbc8eb810f77b6e9600e97f",
"name": "M1_20170521_1745_dsRNA_A2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:05:03"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8042 (max 8043) in '8042'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1251
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.5216e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 42 mean_circ: 0.927715
Ran for 1550 frames Cell Events: 52 mean_circ: 0.93138
Ran for 3050 frames Cell Events: 98 mean_circ: 0.929132
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Ran for 5050 frames Cell Events: 162 mean_circ: 0.927032
Ran for 6550 frames Cell Events: 203 mean_circ: 0.926351
Ran for 7050 frames Cell Events: 214 mean_circ: 0.926269
Ran for 8550 frames Cell Events: 270 mean_circ: 0.927739
Ran for 9550 frames Cell Events: 314 mean_circ: 0.924173
Ran for 10550 frames Cell Events: 354 mean_circ: 0.923239
Ran for 11050 frames Cell Events: 384 mean_circ: 0.922637
Ran for 12050 frames Cell Events: 416 mean_circ: 0.922629
Ran for 12550 frames Cell Events: 440 mean_circ: 0.923415
Ran for 13050 frames Cell Events: 453 mean_circ: 0.923629
Ran for 13550 frames Cell Events: 467 mean_circ: 0.924103
Ran for 14050 frames Cell Events: 489 mean_circ: 0.924384
Ran for 14550 frames Cell Events: 499 mean_circ: 0.924576
Ran for 15550 frames Cell Events: 548 mean_circ: 0.923828
Ran for 17050 frames Cell Events: 608 mean_circ: 0.923716
Ran for 17550 frames Cell Events: 635 mean_circ: 0.924301
Ran for 18550 frames Cell Events: 675 mean_circ: 0.924599
Ran for 19050 frames Cell Events: 693 mean_circ: 0.924811
Ran for 20050 frames Cell Events: 719 mean_circ: 0.925156
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Ran for 37050 frames Cell Events: 1280 mean_circ: 0.928067
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Ran for 63550 frames Cell Events: 2161 mean_circ: 0.928336
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Ran for 64550 frames Cell Events: 2191 mean_circ: 0.928332
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Ran for 66550 frames Cell Events: 2243 mean_circ: 0.928337
Ran for 67050 frames Cell Events: 2257 mean_circ: 0.928328
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Ran for 68550 frames Cell Events: 2304 mean_circ: 0.928253
Ran for 69050 frames Cell Events: 2321 mean_circ: 0.928368
Ran for 70050 frames Cell Events: 2353 mean_circ: 0.928372
Ran for 70550 frames Cell Events: 2366 mean_circ: 0.928215
Ran for 71550 frames Cell Events: 2396 mean_circ: 0.928243
Ran for 72050 frames Cell Events: 2407 mean_circ: 0.928179
Ran for 72550 frames Cell Events: 2423 mean_circ: 0.928294
Ran for 74550 frames Cell Events: 2473 mean_circ: 0.928401
Ran for 75050 frames Cell Events: 2488 mean_circ: 0.928442
Ran for 75550 frames Cell Events: 2498 mean_circ: 0.928479
Ran for 76550 frames Cell Events: 2535 mean_circ: 0.928431
Ran for 77050 frames Cell Events: 2549 mean_circ: 0.928407
Ran for 77550 frames Cell Events: 2568 mean_circ: 0.928393
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Ran for 78550 frames Cell Events: 2597 mean_circ: 0.928251
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Ran for 80050 frames Cell Events: 2643 mean_circ: 0.928283
Ran for 81550 frames Cell Events: 2705 mean_circ: 0.928326
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Ran for 83050 frames Cell Events: 2754 mean_circ: 0.928283
Ran for 83550 frames Cell Events: 2777 mean_circ: 0.928235
Ran for 85050 frames Cell Events: 2857 mean_circ: 0.928376
Ran for 85550 frames Cell Events: 2881 mean_circ: 0.928426
Ran for 86550 frames Cell Events: 2927 mean_circ: 0.9286
Ran for 87550 frames Cell Events: 2969 mean_circ: 0.92863
Ran for 88050 frames Cell Events: 2983 mean_circ: 0.928594
Ran for 88550 frames Cell Events: 2989 mean_circ: 0.928615
Ran for 89050 frames Cell Events: 3000 mean_circ: 0.928636
Ran for 90050 frames Cell Events: 3042 mean_circ: 0.928683
Ran for 90550 frames Cell Events: 3058 mean_circ: 0.928617
Ran for 91050 frames Cell Events: 3075 mean_circ: 0.928532
Ran for 91550 frames Cell Events: 3088 mean_circ: 0.928525
Ran for 92050 frames Cell Events: 3106 mean_circ: 0.928532
Ran for 92550 frames Cell Events: 3123 mean_circ: 0.92857
Ran for 93050 frames Cell Events: 3150 mean_circ: 0.928533
Ran for 94050 frames Cell Events: 3188 mean_circ: 0.928368
Ran for 94550 frames Cell Events: 3205 mean_circ: 0.928381
Ran for 95050 frames Cell Events: 3218 mean_circ: 0.928424
Ran for 96050 frames Cell Events: 3259 mean_circ: 0.928535
Ran for 96550 frames Cell Events: 3280 mean_circ: 0.928619
Ran for 97050 frames Cell Events: 3296 mean_circ: 0.92867
Ran for 98050 frames Cell Events: 3347 mean_circ: 0.928595
Ran for 98550 frames Cell Events: 3360 mean_circ: 0.928572
Ran for 99050 frames Cell Events: 3379 mean_circ: 0.928592
Ran for 99550 frames Cell Events: 3395 mean_circ: 0.928589
Ran for 100050 frames Cell Events: 3416 mean_circ: 0.928349
Ran for 100550 frames Cell Events: 3435 mean_circ: 0.928343
Ran for 101050 frames Cell Events: 3459 mean_circ: 0.928347
Ran for 102050 frames Cell Events: 3500 mean_circ: 0.928233
Ran for 102550 frames Cell Events: 3518 mean_circ: 0.928275
Ran for 103050 frames Cell Events: 3535 mean_circ: 0.928261
Ran for 103550 frames Cell Events: 3558 mean_circ: 0.928238
Ran for 104050 frames Cell Events: 3570 mean_circ: 0.928179
Ran for 104550 frames Cell Events: 3586 mean_circ: 0.928239
Ran for 105050 frames Cell Events: 3613 mean_circ: 0.928174
Ran for 105550 frames Cell Events: 3625 mean_circ: 0.928154
Ran for 106050 frames Cell Events: 3645 mean_circ: 0.928088
Ran for 106550 frames Cell Events: 3671 mean_circ: 0.928147
Ran for 107050 frames Cell Events: 3681 mean_circ: 0.92814
Ran for 108050 frames Cell Events: 3714 mean_circ: 0.928216
Ran for 108550 frames Cell Events: 3719 mean_circ: 0.928215
Ran for 109050 frames Cell Events: 3732 mean_circ: 0.92827
Ran for 109550 frames Cell Events: 3752 mean_circ: 0.928292
Ran for 110050 frames Cell Events: 3787 mean_circ: 0.928267
Ran for 110550 frames Cell Events: 3801 mean_circ: 0.928126
Ran for 111050 frames Cell Events: 3814 mean_circ: 0.928098
Ran for 111550 frames Cell Events: 3826 mean_circ: 0.92799
Ran for 112050 frames Cell Events: 3846 mean_circ: 0.928002
Ran for 112550 frames Cell Events: 3861 mean_circ: 0.927924
Ran for 113050 frames Cell Events: 3870 mean_circ: 0.927922
Ran for 113550 frames Cell Events: 3888 mean_circ: 0.927909
Ran for 114050 frames Cell Events: 3903 mean_circ: 0.927952
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Ran for 115050 frames Cell Events: 3944 mean_circ: 0.92779
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Ran for 116550 frames Cell Events: 4006 mean_circ: 0.927736
Ran for 117050 frames Cell Events: 4022 mean_circ: 0.927686
Ran for 118050 frames Cell Events: 4059 mean_circ: 0.927622
Ran for 118550 frames Cell Events: 4075 mean_circ: 0.927605
Ran for 119050 frames Cell Events: 4088 mean_circ: 0.927642
Ran for 120050 frames Cell Events: 4132 mean_circ: 0.927591
Ran for 120550 frames Cell Events: 4157 mean_circ: 0.927631
Ran for 121050 frames Cell Events: 4177 mean_circ: 0.9277
Ran for 122050 frames Cell Events: 4218 mean_circ: 0.927767
Ran for 123050 frames Cell Events: 4263 mean_circ: 0.927756
Ran for 123550 frames Cell Events: 4287 mean_circ: 0.927772
Ran for 124550 frames Cell Events: 4331 mean_circ: 0.927752
Ran for 125550 frames Cell Events: 4368 mean_circ: 0.927779
Ran for 126050 frames Cell Events: 4379 mean_circ: 0.927794
Ran for 126550 frames Cell Events: 4402 mean_circ: 0.927769
Ran for 128050 frames Cell Events: 4443 mean_circ: 0.927749
Ran for 128550 frames Cell Events: 4467 mean_circ: 0.927705
Ran for 129050 frames Cell Events: 4479 mean_circ: 0.92774
Ran for 129550 frames Cell Events: 4501 mean_circ: 0.927743
Ran for 130050 frames Cell Events: 4516 mean_circ: 0.927776
Ran for 130550 frames Cell Events: 4532 mean_circ: 0.927731
Ran for 131550 frames Cell Events: 4560 mean_circ: 0.927671
Ran for 132050 frames Cell Events: 4578 mean_circ: 0.927669
Ran for 133050 frames Cell Events: 4620 mean_circ: 0.927566
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Ran for 142550 frames Cell Events: 4976 mean_circ: 0.927588
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Ran for 177050 frames Cell Events: 6228 mean_circ: 0.927374
Ran for 177550 frames Cell Events: 6247 mean_circ: 0.927401
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Ran for 182050 frames Cell Events: 6395 mean_circ: 0.927513
Ran for 182550 frames Cell Events: 6413 mean_circ: 0.927482
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Ran for 195050 frames Cell Events: 6837 mean_circ: 0.927192
Ran for 195550 frames Cell Events: 6853 mean_circ: 0.927149
Ran for 196050 frames Cell Events: 6869 mean_circ: 0.927121
Ran for 196550 frames Cell Events: 6885 mean_circ: 0.927161
Ran for 198050 frames Cell Events: 6942 mean_circ: 0.927171
Ran for 198550 frames Cell Events: 6955 mean_circ: 0.927132
Ran for 199050 frames Cell Events: 6968 mean_circ: 0.927127
Ran for 199550 frames Cell Events: 6983 mean_circ: 0.92713
Ran for 200050 frames Cell Events: 6998 mean_circ: 0.927127
Ran for 200550 frames Cell Events: 7014 mean_circ: 0.927136
Ran for 201050 frames Cell Events: 7029 mean_circ: 0.927126
Ran for 202050 frames Cell Events: 7071 mean_circ: 0.927182
Ran for 202550 frames Cell Events: 7092 mean_circ: 0.927169
Ran for 203050 frames Cell Events: 7121 mean_circ: 0.927192
Ran for 203550 frames Cell Events: 7142 mean_circ: 0.927126
Ran for 204050 frames Cell Events: 7164 mean_circ: 0.927162
Ran for 204550 frames Cell Events: 7189 mean_circ: 0.927162
Ran for 206050 frames Cell Events: 7261 mean_circ: 0.927263
Ran for 206550 frames Cell Events: 7287 mean_circ: 0.927288
Ran for 207050 frames Cell Events: 7300 mean_circ: 0.927272
Ran for 207550 frames Cell Events: 7318 mean_circ: 0.927254
Ran for 208550 frames Cell Events: 7352 mean_circ: 0.927283
Ran for 209550 frames Cell Events: 7384 mean_circ: 0.92734
Ran for 211550 frames Cell Events: 7456 mean_circ: 0.927342
Ran for 212050 frames Cell Events: 7479 mean_circ: 0.927326
Ran for 212550 frames Cell Events: 7497 mean_circ: 0.927322
Ran for 214050 frames Cell Events: 7577 mean_circ: 0.927248
Ran for 215050 frames Cell Events: 7613 mean_circ: 0.927252
Ran for 215550 frames Cell Events: 7634 mean_circ: 0.927265
Ran for 216050 frames Cell Events: 7645 mean_circ: 0.927294
Ran for 218550 frames Cell Events: 7763 mean_circ: 0.927093
Ran for 219050 frames Cell Events: 7781 mean_circ: 0.927089
Ran for 220050 frames Cell Events: 7822 mean_circ: 0.927038
Ran for 221050 frames Cell Events: 7859 mean_circ: 0.927045
Ran for 221550 frames Cell Events: 7880 mean_circ: 0.927051
Ran for 223050 frames Cell Events: 7923 mean_circ: 0.927073
Ran for 224050 frames Cell Events: 7962 mean_circ: 0.927115
Ran for 224550 frames Cell Events: 7978 mean_circ: 0.927138
Ran for 225050 frames Cell Events: 7996 mean_circ: 0.927137
Ran for 225550 frames Cell Events: 8008 mean_circ: 0.927161
Ran for 226050 frames Cell Events: 8028 mean_circ: 0.927146



End this measurment..



Stopping time: 5.52273e+008
****************************************
Measurement finished

Mean Circularitiy: 0.927128
Mean Area: 646.162
Mean FL-1 intensity: 3013.79
Mean FL-2 intensity: 4183.25
Mean FL-3 intensity: 0

frames processed: 225372
Total Time: 113311ms
Number of Runs (new bg img) 0
Frames collected: 226469
Skipped frames: 57812
Rescued Frames: 2896780
Samples DAQ: 56617579
Samples written: 113234228
Samples per Channel: 56617114
FPS: 1998.65

Samples per second: 499661
Expected samples/s: 500000

Events: 8043
Events per second: 70.9816
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :318722
# 1 frames :117836
# 2 frames :4545
# 3 frames :1504
# 4 frames :922
# 5 frames :377
# 6 frames :1916
# 7 frames :1859
# 8 frames :1263
# 9 frames :692
# 10 frames :520
# 11 frames :439
# 12 frames :452
# 13 frames :327
# 14 frames :279
# 15 frames :221
# 16 frames :165
# 17 frames :174
# 18 frames :130
# 19 frames :82
# 20 frames :74
# 21 frames :65
# 22 frames :55
# 23 frames :33
# 24 frames :35
# 25 frames :38
# 26 frames :24
# 27 frames :23
# 28 frames :17
# 29 frames :13
# 30 frames :15
# 31 frames :13
# 32 frames :6
# 33 frames :7
# 34 frames :6
# 35 frames :3
# 36 frames :4
# 37 frames :2
# 38 frames :2
# 39 frames :2
# 40 frames :2
# 41 frames :1
# 42 frames :1
# 43 frames :1
# 44 frames :1
# 46 frames :1
# 47 frames :2
# 50 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1251
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0