Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.06.38 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2c1e0cbc418058303d8fd29bbc0de3f6",
"name": "M1_20170521_1809_dsRNA_B2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:07:00"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8261 (max 8262) in '8261'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.3557
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.53582e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 42 mean_circ: 0.929167
Ran for 1550 frames Cell Events: 66 mean_circ: 0.929109
Ran for 2050 frames Cell Events: 94 mean_circ: 0.925522
Ran for 3050 frames Cell Events: 121 mean_circ: 0.922021
Ran for 3550 frames Cell Events: 143 mean_circ: 0.921786
Ran for 4050 frames Cell Events: 161 mean_circ: 0.922929
Ran for 5550 frames Cell Events: 195 mean_circ: 0.92081
Ran for 6550 frames Cell Events: 225 mean_circ: 0.919518
Ran for 7050 frames Cell Events: 243 mean_circ: 0.919057
Ran for 7550 frames Cell Events: 258 mean_circ: 0.920092
Ran for 8050 frames Cell Events: 270 mean_circ: 0.918784
Ran for 9050 frames Cell Events: 302 mean_circ: 0.918472
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Ran for 10050 frames Cell Events: 342 mean_circ: 0.918683
Ran for 10550 frames Cell Events: 352 mean_circ: 0.918708
Ran for 11050 frames Cell Events: 369 mean_circ: 0.918863
Ran for 11550 frames Cell Events: 389 mean_circ: 0.919792
Ran for 12050 frames Cell Events: 407 mean_circ: 0.919613
Ran for 12550 frames Cell Events: 420 mean_circ: 0.919707
Ran for 13550 frames Cell Events: 459 mean_circ: 0.919723
Ran for 14050 frames Cell Events: 475 mean_circ: 0.920105
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Ran for 15550 frames Cell Events: 553 mean_circ: 0.919481
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Ran for 19550 frames Cell Events: 678 mean_circ: 0.920367
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Ran for 58550 frames Cell Events: 2096 mean_circ: 0.919616
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Ran for 64550 frames Cell Events: 2335 mean_circ: 0.919518
Ran for 65050 frames Cell Events: 2352 mean_circ: 0.919584
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Ran for 66550 frames Cell Events: 2413 mean_circ: 0.919467
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Ran for 71050 frames Cell Events: 2554 mean_circ: 0.919667
Ran for 71550 frames Cell Events: 2584 mean_circ: 0.919588
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Ran for 72550 frames Cell Events: 2619 mean_circ: 0.919805
Ran for 73050 frames Cell Events: 2624 mean_circ: 0.919856
Ran for 74050 frames Cell Events: 2657 mean_circ: 0.919881
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Ran for 75050 frames Cell Events: 2683 mean_circ: 0.919925
Ran for 75550 frames Cell Events: 2695 mean_circ: 0.919989
Ran for 76050 frames Cell Events: 2703 mean_circ: 0.920051
Ran for 76550 frames Cell Events: 2721 mean_circ: 0.920085
Ran for 77550 frames Cell Events: 2758 mean_circ: 0.920129
Ran for 78050 frames Cell Events: 2783 mean_circ: 0.920114
Ran for 78550 frames Cell Events: 2798 mean_circ: 0.920191
Ran for 79050 frames Cell Events: 2817 mean_circ: 0.920113
Ran for 79550 frames Cell Events: 2837 mean_circ: 0.920107
Ran for 80550 frames Cell Events: 2875 mean_circ: 0.920299
Ran for 81050 frames Cell Events: 2893 mean_circ: 0.920322
Ran for 81550 frames Cell Events: 2910 mean_circ: 0.920312
Ran for 82550 frames Cell Events: 2946 mean_circ: 0.9203
Ran for 83050 frames Cell Events: 2957 mean_circ: 0.920263
Ran for 84050 frames Cell Events: 2991 mean_circ: 0.920349
Ran for 85050 frames Cell Events: 3030 mean_circ: 0.920593
Ran for 86050 frames Cell Events: 3070 mean_circ: 0.920455
Ran for 86550 frames Cell Events: 3081 mean_circ: 0.920468
Ran for 87050 frames Cell Events: 3103 mean_circ: 0.920401
Ran for 87550 frames Cell Events: 3122 mean_circ: 0.920407
Ran for 88050 frames Cell Events: 3142 mean_circ: 0.920417
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Ran for 90050 frames Cell Events: 3227 mean_circ: 0.920609
Ran for 91050 frames Cell Events: 3266 mean_circ: 0.920788
Ran for 91550 frames Cell Events: 3285 mean_circ: 0.920767
Ran for 92050 frames Cell Events: 3309 mean_circ: 0.920842
Ran for 92550 frames Cell Events: 3328 mean_circ: 0.920912
Ran for 93050 frames Cell Events: 3344 mean_circ: 0.920969
Ran for 94050 frames Cell Events: 3387 mean_circ: 0.921047
Ran for 94550 frames Cell Events: 3401 mean_circ: 0.920997
Ran for 96050 frames Cell Events: 3454 mean_circ: 0.921057
Ran for 97050 frames Cell Events: 3490 mean_circ: 0.920964
Ran for 97550 frames Cell Events: 3506 mean_circ: 0.921003
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Ran for 102050 frames Cell Events: 3690 mean_circ: 0.921137
Ran for 102550 frames Cell Events: 3705 mean_circ: 0.921066
Ran for 104550 frames Cell Events: 3783 mean_circ: 0.921019
Ran for 106050 frames Cell Events: 3859 mean_circ: 0.92117
Ran for 106550 frames Cell Events: 3875 mean_circ: 0.921148
Ran for 107050 frames Cell Events: 3884 mean_circ: 0.921106
Ran for 107550 frames Cell Events: 3896 mean_circ: 0.921128
Ran for 108050 frames Cell Events: 3916 mean_circ: 0.921091
Ran for 108550 frames Cell Events: 3928 mean_circ: 0.921083
Ran for 109050 frames Cell Events: 3943 mean_circ: 0.92108
Ran for 109550 frames Cell Events: 3951 mean_circ: 0.92106
Ran for 110550 frames Cell Events: 3995 mean_circ: 0.920992
Ran for 111050 frames Cell Events: 4016 mean_circ: 0.921051
Ran for 111550 frames Cell Events: 4031 mean_circ: 0.921097
Ran for 112050 frames Cell Events: 4054 mean_circ: 0.921198
Ran for 112550 frames Cell Events: 4070 mean_circ: 0.921198
Ran for 113050 frames Cell Events: 4085 mean_circ: 0.921197
Ran for 113550 frames Cell Events: 4099 mean_circ: 0.921138
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Ran for 120050 frames Cell Events: 4340 mean_circ: 0.920808
Ran for 120550 frames Cell Events: 4361 mean_circ: 0.920783
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Ran for 121550 frames Cell Events: 4404 mean_circ: 0.920644
Ran for 122050 frames Cell Events: 4431 mean_circ: 0.920623
Ran for 122550 frames Cell Events: 4441 mean_circ: 0.920531
Ran for 123050 frames Cell Events: 4462 mean_circ: 0.920471
Ran for 123550 frames Cell Events: 4485 mean_circ: 0.920475
Ran for 125050 frames Cell Events: 4525 mean_circ: 0.920451
Ran for 125550 frames Cell Events: 4550 mean_circ: 0.920428
Ran for 126050 frames Cell Events: 4576 mean_circ: 0.920435
Ran for 126550 frames Cell Events: 4587 mean_circ: 0.920437
Ran for 127550 frames Cell Events: 4634 mean_circ: 0.920454
Ran for 128050 frames Cell Events: 4655 mean_circ: 0.920398
Ran for 129050 frames Cell Events: 4694 mean_circ: 0.920269
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Ran for 146550 frames Cell Events: 5332 mean_circ: 0.920333
Ran for 147050 frames Cell Events: 5350 mean_circ: 0.920347
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Ran for 167050 frames Cell Events: 6031 mean_circ: 0.920495
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Ran for 168050 frames Cell Events: 6066 mean_circ: 0.920515
Ran for 168550 frames Cell Events: 6083 mean_circ: 0.920551
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Ran for 171550 frames Cell Events: 6181 mean_circ: 0.920528
Ran for 172050 frames Cell Events: 6204 mean_circ: 0.920553
Ran for 172550 frames Cell Events: 6224 mean_circ: 0.920558
Ran for 173050 frames Cell Events: 6243 mean_circ: 0.92055
Ran for 173550 frames Cell Events: 6257 mean_circ: 0.920551
Ran for 174050 frames Cell Events: 6270 mean_circ: 0.920596
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Ran for 177050 frames Cell Events: 6387 mean_circ: 0.920562
Ran for 177550 frames Cell Events: 6410 mean_circ: 0.920522
Ran for 178050 frames Cell Events: 6431 mean_circ: 0.920531
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Ran for 181550 frames Cell Events: 6564 mean_circ: 0.920399
Ran for 182050 frames Cell Events: 6581 mean_circ: 0.92043
Ran for 183050 frames Cell Events: 6621 mean_circ: 0.920473
Ran for 183550 frames Cell Events: 6641 mean_circ: 0.920496
Ran for 184050 frames Cell Events: 6661 mean_circ: 0.92047
Ran for 184550 frames Cell Events: 6675 mean_circ: 0.920493
Ran for 185050 frames Cell Events: 6693 mean_circ: 0.92047
Ran for 187050 frames Cell Events: 6750 mean_circ: 0.920376
Ran for 187550 frames Cell Events: 6772 mean_circ: 0.920349
Ran for 188050 frames Cell Events: 6793 mean_circ: 0.92034
Ran for 188550 frames Cell Events: 6825 mean_circ: 0.920345
Ran for 189550 frames Cell Events: 6856 mean_circ: 0.920367
Ran for 190050 frames Cell Events: 6873 mean_circ: 0.920397
Ran for 190550 frames Cell Events: 6895 mean_circ: 0.920431
Ran for 191550 frames Cell Events: 6948 mean_circ: 0.920505
Ran for 193050 frames Cell Events: 7008 mean_circ: 0.920458
Ran for 193550 frames Cell Events: 7028 mean_circ: 0.920447
Ran for 195550 frames Cell Events: 7114 mean_circ: 0.920423
Ran for 196550 frames Cell Events: 7153 mean_circ: 0.920446
Ran for 197550 frames Cell Events: 7180 mean_circ: 0.92042
Ran for 199050 frames Cell Events: 7232 mean_circ: 0.920435
Ran for 199550 frames Cell Events: 7249 mean_circ: 0.920402
Ran for 202050 frames Cell Events: 7346 mean_circ: 0.920326
Ran for 202550 frames Cell Events: 7361 mean_circ: 0.920273
Ran for 203550 frames Cell Events: 7400 mean_circ: 0.920257
Ran for 204050 frames Cell Events: 7424 mean_circ: 0.920248
Ran for 205050 frames Cell Events: 7461 mean_circ: 0.920297
Ran for 205550 frames Cell Events: 7482 mean_circ: 0.920294
Ran for 206050 frames Cell Events: 7503 mean_circ: 0.920292
Ran for 206550 frames Cell Events: 7517 mean_circ: 0.920296
Ran for 207050 frames Cell Events: 7534 mean_circ: 0.92029
Ran for 207550 frames Cell Events: 7561 mean_circ: 0.92034
Ran for 209050 frames Cell Events: 7598 mean_circ: 0.920293
Ran for 209550 frames Cell Events: 7612 mean_circ: 0.92029
Ran for 210050 frames Cell Events: 7629 mean_circ: 0.920256
Ran for 210550 frames Cell Events: 7645 mean_circ: 0.920216
Ran for 211050 frames Cell Events: 7671 mean_circ: 0.920224
Ran for 212050 frames Cell Events: 7710 mean_circ: 0.920228
Ran for 212550 frames Cell Events: 7719 mean_circ: 0.920218
Ran for 213050 frames Cell Events: 7736 mean_circ: 0.920225
Ran for 213550 frames Cell Events: 7752 mean_circ: 0.920219
Ran for 214050 frames Cell Events: 7765 mean_circ: 0.920139
Ran for 214550 frames Cell Events: 7785 mean_circ: 0.920113
Ran for 216050 frames Cell Events: 7837 mean_circ: 0.920129
Ran for 216550 frames Cell Events: 7853 mean_circ: 0.920139
Ran for 217050 frames Cell Events: 7871 mean_circ: 0.920122
Ran for 217550 frames Cell Events: 7883 mean_circ: 0.920124
Ran for 218050 frames Cell Events: 7906 mean_circ: 0.920127
Ran for 219050 frames Cell Events: 7944 mean_circ: 0.920035
Ran for 219550 frames Cell Events: 7956 mean_circ: 0.920056
Ran for 220050 frames Cell Events: 7987 mean_circ: 0.920039
Ran for 221050 frames Cell Events: 8029 mean_circ: 0.920054
Ran for 221550 frames Cell Events: 8058 mean_circ: 0.920073
Ran for 222050 frames Cell Events: 8086 mean_circ: 0.920086
Ran for 222550 frames Cell Events: 8097 mean_circ: 0.920088
Ran for 223050 frames Cell Events: 8104 mean_circ: 0.920057
Ran for 223550 frames Cell Events: 8118 mean_circ: 0.920059
Ran for 224050 frames Cell Events: 8140 mean_circ: 0.920044
Ran for 224550 frames Cell Events: 8149 mean_circ: 0.920037
Ran for 225050 frames Cell Events: 8169 mean_circ: 0.920021
Ran for 225550 frames Cell Events: 8192 mean_circ: 0.920045
Ran for 226050 frames Cell Events: 8214 mean_circ: 0.920032
Ran for 226550 frames Cell Events: 8238 mean_circ: 0.920018



End this measurment..



Stopping time: 5.53696e+008
****************************************
Measurement finished

Mean Circularitiy: 0.920006
Mean Area: 644.339
Mean FL-1 intensity: 3590.95
Mean FL-2 intensity: 5079.37
Mean FL-3 intensity: 0

frames processed: 224957
Total Time: 113625ms
Number of Runs (new bg img) 0
Frames collected: 227113
Skipped frames: 66310
Rescued Frames: 3268459
Samples DAQ: 56778263
Samples written: 113556446
Samples per Channel: 56778223
FPS: 1998.79

Samples per second: 499697
Expected samples/s: 500000

Events: 8262
Events per second: 72.7127
jumped back (PMT): 11
#Frames read, frequency
# 0 frames :310749
# 1 frames :127415
# 2 frames :4575
# 3 frames :1455
# 4 frames :633
# 5 frames :208
# 6 frames :3002
# 7 frames :1818
# 8 frames :1273
# 9 frames :604
# 10 frames :516
# 11 frames :366
# 12 frames :360
# 13 frames :233
# 14 frames :238
# 15 frames :161
# 16 frames :124
# 17 frames :108
# 18 frames :63
# 19 frames :41
# 20 frames :52
# 21 frames :28
# 22 frames :34
# 23 frames :24
# 24 frames :13
# 25 frames :17
# 26 frames :13
# 27 frames :10
# 28 frames :7
# 29 frames :10
# 30 frames :5
# 31 frames :5
# 32 frames :6
# 33 frames :8
# 34 frames :4
# 35 frames :1
# 36 frames :3
# 37 frames :1
# 38 frames :1
# 39 frames :1
# 41 frames :1
# 42 frames :2
# 44 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.3557
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0