Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.17.31 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e500d7d7d7221dab94114c27a04aba2a",
"name": "M1_20170522_1312_nk1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:17:51"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8040 (max 8041) in '8040'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.7127
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.22192e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 40 mean_circ: 0.931103
Ran for 2050 frames Cell Events: 57 mean_circ: 0.922781
Ran for 3050 frames Cell Events: 106 mean_circ: 0.921665
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Ran for 4550 frames Cell Events: 166 mean_circ: 0.92619
Ran for 5050 frames Cell Events: 182 mean_circ: 0.926688
Ran for 5550 frames Cell Events: 197 mean_circ: 0.927221
Ran for 6050 frames Cell Events: 215 mean_circ: 0.925507
Ran for 7050 frames Cell Events: 261 mean_circ: 0.927663
Ran for 8550 frames Cell Events: 335 mean_circ: 0.925029
Ran for 10050 frames Cell Events: 394 mean_circ: 0.925419
Ran for 10550 frames Cell Events: 407 mean_circ: 0.925384
Ran for 11050 frames Cell Events: 429 mean_circ: 0.92572
Ran for 11550 frames Cell Events: 450 mean_circ: 0.926013
Ran for 12050 frames Cell Events: 472 mean_circ: 0.925676
Ran for 12550 frames Cell Events: 492 mean_circ: 0.926433
Ran for 14050 frames Cell Events: 541 mean_circ: 0.92736
Ran for 15050 frames Cell Events: 569 mean_circ: 0.927554
Ran for 15550 frames Cell Events: 581 mean_circ: 0.927649
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Ran for 16550 frames Cell Events: 606 mean_circ: 0.927815
Ran for 17550 frames Cell Events: 657 mean_circ: 0.928082
Ran for 18050 frames Cell Events: 674 mean_circ: 0.928249
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Ran for 23550 frames Cell Events: 849 mean_circ: 0.92859
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Ran for 35050 frames Cell Events: 1256 mean_circ: 0.927567
Ran for 35550 frames Cell Events: 1275 mean_circ: 0.927607
Ran for 36050 frames Cell Events: 1295 mean_circ: 0.927554
Ran for 36550 frames Cell Events: 1324 mean_circ: 0.927637
Ran for 37050 frames Cell Events: 1340 mean_circ: 0.927688
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Ran for 63550 frames Cell Events: 2214 mean_circ: 0.927232
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Ran for 64550 frames Cell Events: 2214 mean_circ: 0.927232
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Ran for 67050 frames Cell Events: 2268 mean_circ: 0.919813
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Ran for 68050 frames Cell Events: 2282 mean_circ: 0.919809
Ran for 68550 frames Cell Events: 2307 mean_circ: 0.919585
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Ran for 70550 frames Cell Events: 2399 mean_circ: 0.919829
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Ran for 71550 frames Cell Events: 2440 mean_circ: 0.919906
Ran for 72550 frames Cell Events: 2491 mean_circ: 0.919878
Ran for 74050 frames Cell Events: 2549 mean_circ: 0.920174
Ran for 74550 frames Cell Events: 2570 mean_circ: 0.920294
Ran for 75050 frames Cell Events: 2586 mean_circ: 0.920457
Ran for 75550 frames Cell Events: 2592 mean_circ: 0.920514
Ran for 76050 frames Cell Events: 2618 mean_circ: 0.920547
Ran for 76550 frames Cell Events: 2645 mean_circ: 0.920468
Ran for 77050 frames Cell Events: 2659 mean_circ: 0.920272
Ran for 77550 frames Cell Events: 2683 mean_circ: 0.920317
Ran for 78050 frames Cell Events: 2697 mean_circ: 0.920408
Ran for 78550 frames Cell Events: 2725 mean_circ: 0.920516
Ran for 79050 frames Cell Events: 2750 mean_circ: 0.920482
Ran for 80050 frames Cell Events: 2785 mean_circ: 0.920579
Ran for 80550 frames Cell Events: 2815 mean_circ: 0.920596
Ran for 81050 frames Cell Events: 2832 mean_circ: 0.920649
Ran for 81550 frames Cell Events: 2848 mean_circ: 0.920637
Ran for 82050 frames Cell Events: 2860 mean_circ: 0.920713
Ran for 83050 frames Cell Events: 2899 mean_circ: 0.920773
Ran for 83550 frames Cell Events: 2910 mean_circ: 0.920773
Ran for 84050 frames Cell Events: 2921 mean_circ: 0.920801
Ran for 84550 frames Cell Events: 2937 mean_circ: 0.920841
Ran for 85050 frames Cell Events: 2957 mean_circ: 0.920885
Ran for 86050 frames Cell Events: 2993 mean_circ: 0.921161
Ran for 86550 frames Cell Events: 3014 mean_circ: 0.921255
Ran for 87050 frames Cell Events: 3041 mean_circ: 0.921341
Ran for 88050 frames Cell Events: 3066 mean_circ: 0.921382
Ran for 88550 frames Cell Events: 3081 mean_circ: 0.921387
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Ran for 90050 frames Cell Events: 3129 mean_circ: 0.921508
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Ran for 91050 frames Cell Events: 3162 mean_circ: 0.921663
Ran for 92050 frames Cell Events: 3196 mean_circ: 0.9217
Ran for 92550 frames Cell Events: 3218 mean_circ: 0.921623
Ran for 94050 frames Cell Events: 3262 mean_circ: 0.921709
Ran for 94550 frames Cell Events: 3280 mean_circ: 0.921639
Ran for 95050 frames Cell Events: 3308 mean_circ: 0.921654
Ran for 95550 frames Cell Events: 3325 mean_circ: 0.921647
Ran for 96050 frames Cell Events: 3343 mean_circ: 0.921657
Ran for 96550 frames Cell Events: 3367 mean_circ: 0.921522
Ran for 97050 frames Cell Events: 3389 mean_circ: 0.921431
Ran for 98050 frames Cell Events: 3431 mean_circ: 0.921375
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Ran for 99550 frames Cell Events: 3506 mean_circ: 0.921456
Ran for 100050 frames Cell Events: 3527 mean_circ: 0.921495
Ran for 100550 frames Cell Events: 3533 mean_circ: 0.921545
Ran for 101050 frames Cell Events: 3542 mean_circ: 0.921563
Ran for 102550 frames Cell Events: 3605 mean_circ: 0.921625
Ran for 103550 frames Cell Events: 3649 mean_circ: 0.921718
Ran for 104050 frames Cell Events: 3674 mean_circ: 0.921829
Ran for 106050 frames Cell Events: 3741 mean_circ: 0.921857
Ran for 107050 frames Cell Events: 3767 mean_circ: 0.92194
Ran for 107550 frames Cell Events: 3779 mean_circ: 0.921946
Ran for 108050 frames Cell Events: 3799 mean_circ: 0.921996
Ran for 108550 frames Cell Events: 3811 mean_circ: 0.921981
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Ran for 109550 frames Cell Events: 3845 mean_circ: 0.921955
Ran for 110050 frames Cell Events: 3861 mean_circ: 0.922043
Ran for 111550 frames Cell Events: 3911 mean_circ: 0.922206
Ran for 112050 frames Cell Events: 3918 mean_circ: 0.922193
Ran for 113050 frames Cell Events: 3952 mean_circ: 0.92229
Ran for 115550 frames Cell Events: 4087 mean_circ: 0.922029
Ran for 116050 frames Cell Events: 4105 mean_circ: 0.922013
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Ran for 120050 frames Cell Events: 4238 mean_circ: 0.922108
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Ran for 122050 frames Cell Events: 4324 mean_circ: 0.922195
Ran for 122550 frames Cell Events: 4344 mean_circ: 0.922231
Ran for 123050 frames Cell Events: 4362 mean_circ: 0.922251
Ran for 124050 frames Cell Events: 4399 mean_circ: 0.922293
Ran for 124550 frames Cell Events: 4409 mean_circ: 0.922314
Ran for 125050 frames Cell Events: 4428 mean_circ: 0.922307
Ran for 125550 frames Cell Events: 4453 mean_circ: 0.922329
Ran for 126050 frames Cell Events: 4470 mean_circ: 0.922286
Ran for 126550 frames Cell Events: 4487 mean_circ: 0.922323
Ran for 127050 frames Cell Events: 4502 mean_circ: 0.922371
Ran for 128550 frames Cell Events: 4559 mean_circ: 0.922403
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Ran for 130550 frames Cell Events: 4639 mean_circ: 0.922435
Ran for 131050 frames Cell Events: 4658 mean_circ: 0.922505
Ran for 131550 frames Cell Events: 4672 mean_circ: 0.922444
Ran for 132050 frames Cell Events: 4692 mean_circ: 0.922553
Ran for 132550 frames Cell Events: 4718 mean_circ: 0.922652
Ran for 133050 frames Cell Events: 4736 mean_circ: 0.922648
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Ran for 158050 frames Cell Events: 5723 mean_circ: 0.923164
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Ran for 160050 frames Cell Events: 5809 mean_circ: 0.923223
Ran for 160550 frames Cell Events: 5832 mean_circ: 0.923234
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Ran for 162550 frames Cell Events: 5925 mean_circ: 0.923155
Ran for 163050 frames Cell Events: 5942 mean_circ: 0.923196
Ran for 164550 frames Cell Events: 6014 mean_circ: 0.92321
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Ran for 166550 frames Cell Events: 6091 mean_circ: 0.923235
Ran for 167550 frames Cell Events: 6134 mean_circ: 0.923217
Ran for 168050 frames Cell Events: 6168 mean_circ: 0.923279
Ran for 168550 frames Cell Events: 6184 mean_circ: 0.923293
Ran for 169050 frames Cell Events: 6201 mean_circ: 0.923316
Ran for 170050 frames Cell Events: 6241 mean_circ: 0.923429
Ran for 171050 frames Cell Events: 6288 mean_circ: 0.92338
Ran for 171550 frames Cell Events: 6311 mean_circ: 0.923297
Ran for 172050 frames Cell Events: 6330 mean_circ: 0.923291
Ran for 173550 frames Cell Events: 6393 mean_circ: 0.92333
Ran for 174050 frames Cell Events: 6419 mean_circ: 0.92328
Ran for 174550 frames Cell Events: 6436 mean_circ: 0.923271
Ran for 175050 frames Cell Events: 6457 mean_circ: 0.92331
Ran for 175550 frames Cell Events: 6480 mean_circ: 0.923262
Ran for 176550 frames Cell Events: 6531 mean_circ: 0.923323
Ran for 177050 frames Cell Events: 6552 mean_circ: 0.923313
Ran for 178050 frames Cell Events: 6606 mean_circ: 0.923255
Ran for 178550 frames Cell Events: 6640 mean_circ: 0.923167
Ran for 179550 frames Cell Events: 6674 mean_circ: 0.923156
Ran for 180550 frames Cell Events: 6727 mean_circ: 0.923205
Ran for 181550 frames Cell Events: 6768 mean_circ: 0.923148
Ran for 182050 frames Cell Events: 6783 mean_circ: 0.923139
Ran for 183550 frames Cell Events: 6842 mean_circ: 0.923129
Ran for 185050 frames Cell Events: 6901 mean_circ: 0.923141
Ran for 185550 frames Cell Events: 6923 mean_circ: 0.923122
Ran for 186050 frames Cell Events: 6951 mean_circ: 0.923239
Ran for 186550 frames Cell Events: 6971 mean_circ: 0.923239
Ran for 187050 frames Cell Events: 6988 mean_circ: 0.923283
Ran for 187550 frames Cell Events: 7005 mean_circ: 0.923285
Ran for 188050 frames Cell Events: 7024 mean_circ: 0.923289
Ran for 188550 frames Cell Events: 7040 mean_circ: 0.923331
Ran for 189050 frames Cell Events: 7057 mean_circ: 0.923332
Ran for 189550 frames Cell Events: 7084 mean_circ: 0.923389
Ran for 190050 frames Cell Events: 7100 mean_circ: 0.923377
Ran for 190550 frames Cell Events: 7111 mean_circ: 0.923408
Ran for 192550 frames Cell Events: 7205 mean_circ: 0.923501
Ran for 193050 frames Cell Events: 7231 mean_circ: 0.923472
Ran for 193550 frames Cell Events: 7245 mean_circ: 0.923424
Ran for 195050 frames Cell Events: 7348 mean_circ: 0.923353
Ran for 195550 frames Cell Events: 7374 mean_circ: 0.923382
Ran for 196050 frames Cell Events: 7397 mean_circ: 0.92342
Ran for 197550 frames Cell Events: 7462 mean_circ: 0.92347
Ran for 198550 frames Cell Events: 7507 mean_circ: 0.923577
Ran for 199050 frames Cell Events: 7521 mean_circ: 0.923609
Ran for 199550 frames Cell Events: 7537 mean_circ: 0.923628
Ran for 200050 frames Cell Events: 7560 mean_circ: 0.923654
Ran for 201050 frames Cell Events: 7595 mean_circ: 0.923668
Ran for 202550 frames Cell Events: 7672 mean_circ: 0.923718
Ran for 203050 frames Cell Events: 7692 mean_circ: 0.923725
Ran for 203550 frames Cell Events: 7710 mean_circ: 0.923725
Ran for 204550 frames Cell Events: 7749 mean_circ: 0.923738
Ran for 205050 frames Cell Events: 7754 mean_circ: 0.923703
Ran for 205550 frames Cell Events: 7775 mean_circ: 0.923711
Ran for 206050 frames Cell Events: 7790 mean_circ: 0.92371
Ran for 207050 frames Cell Events: 7828 mean_circ: 0.923706
Ran for 207550 frames Cell Events: 7847 mean_circ: 0.923714
Ran for 208050 frames Cell Events: 7871 mean_circ: 0.923724
Ran for 208550 frames Cell Events: 7889 mean_circ: 0.923675
Ran for 209550 frames Cell Events: 7941 mean_circ: 0.923634
Ran for 210050 frames Cell Events: 7963 mean_circ: 0.923659
Ran for 211050 frames Cell Events: 8004 mean_circ: 0.923708



End this measurment..



Stopping time: 6.22298e+008
****************************************
Measurement finished

Mean Circularitiy: 0.923665
Mean Area: 628.693
Mean FL-1 intensity: 3002.3
Mean FL-2 intensity: 4703.64
Mean FL-3 intensity: 0

frames processed: 209698
Total Time: 106031ms
Number of Runs (new bg img) 0
Frames collected: 211898
Skipped frames: 3349
Rescued Frames: 170372
Samples DAQ: 52974540
Samples written: 105948814
Samples per Channel: 52974407
FPS: 1998.45

Samples per second: 499611
Expected samples/s: 500000

Events: 8041
Events per second: 75.836
jumped back (PMT): 13
#Frames read, frequency
# 0 frames :294661
# 1 frames :113967
# 2 frames :4069
# 3 frames :1447
# 4 frames :645
# 5 frames :277
# 6 frames :2704
# 7 frames :1476
# 8 frames :1184
# 9 frames :566
# 10 frames :701
# 11 frames :416
# 12 frames :376
# 13 frames :211
# 14 frames :243
# 15 frames :160
# 16 frames :123
# 17 frames :97
# 18 frames :75
# 19 frames :57
# 20 frames :60
# 21 frames :37
# 22 frames :34
# 23 frames :27
# 24 frames :29
# 25 frames :19
# 26 frames :15
# 27 frames :12
# 28 frames :12
# 29 frames :5
# 30 frames :8
# 31 frames :10
# 32 frames :6
# 33 frames :5
# 34 frames :9
# 35 frames :4
# 36 frames :1
# 37 frames :2
# 38 frames :1
# 39 frames :1
# 43 frames :3
# 45 frames :1
# 46 frames :1
# 52 frames :1
# 55 frames :2
# 57 frames :1
# 61 frames :1
# 97 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.7127
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0