Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.23.19 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f335412060f57d0c5b2cb925cd625920",
"name": "M1_20170522_1423_dsRNA_C7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:23:45"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9999 (max 10000) in
'9999'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.2565
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.2644e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 19 mean_circ: 0.94658
Ran for 1050 frames Cell Events: 37 mean_circ: 0.948286
Ran for 2050 frames Cell Events: 85 mean_circ: 0.947773
Ran for 2550 frames Cell Events: 100 mean_circ: 0.946013
Ran for 3050 frames Cell Events: 132 mean_circ: 0.945238
Ran for 3550 frames Cell Events: 162 mean_circ: 0.945094
Ran for 4050 frames Cell Events: 184 mean_circ: 0.945372
Ran for 5550 frames Cell Events: 250 mean_circ: 0.945169
Ran for 7050 frames Cell Events: 335 mean_circ: 0.943245
Ran for 7550 frames Cell Events: 359 mean_circ: 0.942542
Ran for 8050 frames Cell Events: 371 mean_circ: 0.942845
Ran for 9550 frames Cell Events: 454 mean_circ: 0.942952
Ran for 10050 frames Cell Events: 472 mean_circ: 0.942824
Ran for 10550 frames Cell Events: 489 mean_circ: 0.942731
Ran for 11550 frames Cell Events: 527 mean_circ: 0.94299
Ran for 12050 frames Cell Events: 549 mean_circ: 0.942219
Ran for 12550 frames Cell Events: 568 mean_circ: 0.941928
Ran for 13050 frames Cell Events: 592 mean_circ: 0.941339
Ran for 14550 frames Cell Events: 641 mean_circ: 0.94117
Ran for 15550 frames Cell Events: 691 mean_circ: 0.941036
Ran for 16050 frames Cell Events: 715 mean_circ: 0.940916
Ran for 16550 frames Cell Events: 747 mean_circ: 0.94094
Ran for 17050 frames Cell Events: 768 mean_circ: 0.940913
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Ran for 22050 frames Cell Events: 1004 mean_circ: 0.94078
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Ran for 23550 frames Cell Events: 1079 mean_circ: 0.940673
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Ran for 24550 frames Cell Events: 1124 mean_circ: 0.940841
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Ran for 37050 frames Cell Events: 1633 mean_circ: 0.940211
Ran for 37550 frames Cell Events: 1649 mean_circ: 0.939989
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Ran for 39550 frames Cell Events: 1743 mean_circ: 0.939999
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Ran for 61050 frames Cell Events: 2682 mean_circ: 0.940297
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Ran for 63050 frames Cell Events: 2765 mean_circ: 0.940116
Ran for 63550 frames Cell Events: 2784 mean_circ: 0.940059
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Ran for 64550 frames Cell Events: 2821 mean_circ: 0.940051
Ran for 66050 frames Cell Events: 2885 mean_circ: 0.940019
Ran for 66550 frames Cell Events: 2917 mean_circ: 0.939972
Ran for 67050 frames Cell Events: 2940 mean_circ: 0.939996
Ran for 67550 frames Cell Events: 2970 mean_circ: 0.940017
Ran for 68050 frames Cell Events: 2997 mean_circ: 0.940152
Ran for 68550 frames Cell Events: 3022 mean_circ: 0.940191
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Ran for 70050 frames Cell Events: 3083 mean_circ: 0.94021
Ran for 71050 frames Cell Events: 3129 mean_circ: 0.940341
Ran for 72550 frames Cell Events: 3204 mean_circ: 0.94034
Ran for 73050 frames Cell Events: 3227 mean_circ: 0.940332
Ran for 73550 frames Cell Events: 3245 mean_circ: 0.940336
Ran for 74550 frames Cell Events: 3282 mean_circ: 0.940239
Ran for 75550 frames Cell Events: 3319 mean_circ: 0.940324
Ran for 76050 frames Cell Events: 3333 mean_circ: 0.940347
Ran for 77550 frames Cell Events: 3421 mean_circ: 0.940278
Ran for 78050 frames Cell Events: 3442 mean_circ: 0.940211
Ran for 78550 frames Cell Events: 3464 mean_circ: 0.940261
Ran for 79550 frames Cell Events: 3506 mean_circ: 0.940345
Ran for 80050 frames Cell Events: 3527 mean_circ: 0.940346
Ran for 80550 frames Cell Events: 3550 mean_circ: 0.940387
Ran for 81550 frames Cell Events: 3583 mean_circ: 0.940414
Ran for 82550 frames Cell Events: 3636 mean_circ: 0.940378
Ran for 84050 frames Cell Events: 3713 mean_circ: 0.940351
Ran for 85050 frames Cell Events: 3730 mean_circ: 0.940358
Ran for 85550 frames Cell Events: 3740 mean_circ: 0.940354
Ran for 87050 frames Cell Events: 3810 mean_circ: 0.94034
Ran for 87550 frames Cell Events: 3829 mean_circ: 0.940343
Ran for 88550 frames Cell Events: 3880 mean_circ: 0.940223
Ran for 90550 frames Cell Events: 3975 mean_circ: 0.940067
Ran for 91050 frames Cell Events: 3998 mean_circ: 0.940051
Ran for 92050 frames Cell Events: 4039 mean_circ: 0.940075
Ran for 92550 frames Cell Events: 4060 mean_circ: 0.939971
Ran for 94550 frames Cell Events: 4137 mean_circ: 0.939926
Ran for 95050 frames Cell Events: 4161 mean_circ: 0.939945
Ran for 95550 frames Cell Events: 4186 mean_circ: 0.940005
Ran for 96550 frames Cell Events: 4229 mean_circ: 0.93995
Ran for 97550 frames Cell Events: 4276 mean_circ: 0.939866
Ran for 98050 frames Cell Events: 4297 mean_circ: 0.939821
Ran for 99050 frames Cell Events: 4339 mean_circ: 0.93977
Ran for 100550 frames Cell Events: 4406 mean_circ: 0.939883
Ran for 101550 frames Cell Events: 4455 mean_circ: 0.93989
Ran for 102550 frames Cell Events: 4498 mean_circ: 0.9399
Ran for 103550 frames Cell Events: 4542 mean_circ: 0.939848
Ran for 104050 frames Cell Events: 4565 mean_circ: 0.939744
Ran for 106050 frames Cell Events: 4658 mean_circ: 0.939619
Ran for 106550 frames Cell Events: 4679 mean_circ: 0.939669
Ran for 107050 frames Cell Events: 4694 mean_circ: 0.939727
Ran for 109050 frames Cell Events: 4779 mean_circ: 0.939725
Ran for 109550 frames Cell Events: 4803 mean_circ: 0.939716
Ran for 110050 frames Cell Events: 4828 mean_circ: 0.939666
Ran for 110550 frames Cell Events: 4851 mean_circ: 0.939721
Ran for 111550 frames Cell Events: 4896 mean_circ: 0.939632
Ran for 112050 frames Cell Events: 4922 mean_circ: 0.939635
Ran for 112550 frames Cell Events: 4940 mean_circ: 0.939607
Ran for 113550 frames Cell Events: 4978 mean_circ: 0.939668
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Ran for 115550 frames Cell Events: 5080 mean_circ: 0.939805
Ran for 116050 frames Cell Events: 5100 mean_circ: 0.939693
Ran for 116550 frames Cell Events: 5113 mean_circ: 0.939692
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Ran for 117550 frames Cell Events: 5160 mean_circ: 0.939705
Ran for 118050 frames Cell Events: 5174 mean_circ: 0.93974
Ran for 119550 frames Cell Events: 5253 mean_circ: 0.939686
Ran for 120050 frames Cell Events: 5272 mean_circ: 0.939552
Ran for 120550 frames Cell Events: 5301 mean_circ: 0.939561
Ran for 121550 frames Cell Events: 5344 mean_circ: 0.939505
Ran for 122050 frames Cell Events: 5362 mean_circ: 0.939499
Ran for 122550 frames Cell Events: 5393 mean_circ: 0.939454
Ran for 123550 frames Cell Events: 5432 mean_circ: 0.939394
Ran for 124050 frames Cell Events: 5456 mean_circ: 0.939423
Ran for 124550 frames Cell Events: 5482 mean_circ: 0.939486
Ran for 125550 frames Cell Events: 5528 mean_circ: 0.939427
Ran for 126050 frames Cell Events: 5554 mean_circ: 0.939441
Ran for 126550 frames Cell Events: 5577 mean_circ: 0.939385
Ran for 127050 frames Cell Events: 5598 mean_circ: 0.939401
Ran for 128050 frames Cell Events: 5657 mean_circ: 0.939442
Ran for 129050 frames Cell Events: 5709 mean_circ: 0.939461
Ran for 130550 frames Cell Events: 5799 mean_circ: 0.93953
Ran for 131050 frames Cell Events: 5820 mean_circ: 0.939483
Ran for 131550 frames Cell Events: 5834 mean_circ: 0.939422
Ran for 132050 frames Cell Events: 5857 mean_circ: 0.93935
Ran for 132550 frames Cell Events: 5884 mean_circ: 0.939314
Ran for 133050 frames Cell Events: 5915 mean_circ: 0.939367
Ran for 133550 frames Cell Events: 5934 mean_circ: 0.939351
Ran for 135050 frames Cell Events: 6011 mean_circ: 0.93932
Ran for 136550 frames Cell Events: 6076 mean_circ: 0.939298
Ran for 137050 frames Cell Events: 6108 mean_circ: 0.939282
Ran for 137550 frames Cell Events: 6129 mean_circ: 0.939338
Ran for 138050 frames Cell Events: 6150 mean_circ: 0.939352
Ran for 138550 frames Cell Events: 6176 mean_circ: 0.939396
Ran for 139050 frames Cell Events: 6206 mean_circ: 0.93936
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Ran for 140550 frames Cell Events: 6274 mean_circ: 0.939245
Ran for 141050 frames Cell Events: 6297 mean_circ: 0.939228
Ran for 141550 frames Cell Events: 6330 mean_circ: 0.939206
Ran for 142050 frames Cell Events: 6357 mean_circ: 0.939165
Ran for 142550 frames Cell Events: 6383 mean_circ: 0.939165
Ran for 143550 frames Cell Events: 6443 mean_circ: 0.939157
Ran for 144550 frames Cell Events: 6487 mean_circ: 0.939079
Ran for 145050 frames Cell Events: 6510 mean_circ: 0.939074
Ran for 145550 frames Cell Events: 6539 mean_circ: 0.93906
Ran for 146050 frames Cell Events: 6559 mean_circ: 0.9391
Ran for 147050 frames Cell Events: 6604 mean_circ: 0.939045
Ran for 147550 frames Cell Events: 6632 mean_circ: 0.939011
Ran for 149050 frames Cell Events: 6711 mean_circ: 0.939024
Ran for 149550 frames Cell Events: 6727 mean_circ: 0.939044
Ran for 150050 frames Cell Events: 6750 mean_circ: 0.939059
Ran for 150550 frames Cell Events: 6771 mean_circ: 0.939081
Ran for 151050 frames Cell Events: 6793 mean_circ: 0.939082
Ran for 152050 frames Cell Events: 6849 mean_circ: 0.939014
Ran for 152550 frames Cell Events: 6866 mean_circ: 0.938979
Ran for 153550 frames Cell Events: 6906 mean_circ: 0.939006
Ran for 154050 frames Cell Events: 6925 mean_circ: 0.939028
Ran for 154550 frames Cell Events: 6951 mean_circ: 0.93903
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Ran for 156550 frames Cell Events: 7030 mean_circ: 0.939004
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Ran for 158050 frames Cell Events: 7081 mean_circ: 0.938935
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Ran for 172550 frames Cell Events: 7759 mean_circ: 0.938908
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Ran for 182050 frames Cell Events: 8242 mean_circ: 0.939006
Ran for 182550 frames Cell Events: 8261 mean_circ: 0.939
Ran for 183550 frames Cell Events: 8291 mean_circ: 0.938973
Ran for 184550 frames Cell Events: 8334 mean_circ: 0.938997
Ran for 185050 frames Cell Events: 8360 mean_circ: 0.939014
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Ran for 190050 frames Cell Events: 8601 mean_circ: 0.938963
Ran for 190550 frames Cell Events: 8617 mean_circ: 0.938972
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Ran for 192050 frames Cell Events: 8706 mean_circ: 0.939043
Ran for 192550 frames Cell Events: 8728 mean_circ: 0.939054
Ran for 193550 frames Cell Events: 8769 mean_circ: 0.93903
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Ran for 200550 frames Cell Events: 9116 mean_circ: 0.939026
Ran for 201050 frames Cell Events: 9143 mean_circ: 0.939026
Ran for 201550 frames Cell Events: 9162 mean_circ: 0.939052
Ran for 202050 frames Cell Events: 9185 mean_circ: 0.939051
Ran for 202550 frames Cell Events: 9200 mean_circ: 0.939049
Ran for 203050 frames Cell Events: 9224 mean_circ: 0.938993
Ran for 203550 frames Cell Events: 9256 mean_circ: 0.938994
Ran for 204050 frames Cell Events: 9280 mean_circ: 0.93899
Ran for 205050 frames Cell Events: 9333 mean_circ: 0.939005
Ran for 205550 frames Cell Events: 9357 mean_circ: 0.938996
Ran for 207550 frames Cell Events: 9490 mean_circ: 0.938953
Ran for 208050 frames Cell Events: 9517 mean_circ: 0.938929
Ran for 209050 frames Cell Events: 9568 mean_circ: 0.938932
Ran for 209550 frames Cell Events: 9588 mean_circ: 0.938949
Ran for 210050 frames Cell Events: 9610 mean_circ: 0.93898
Ran for 210550 frames Cell Events: 9639 mean_circ: 0.93901
Ran for 211050 frames Cell Events: 9662 mean_circ: 0.938994
Ran for 211550 frames Cell Events: 9682 mean_circ: 0.938996
Ran for 213550 frames Cell Events: 9797 mean_circ: 0.938951
Ran for 214050 frames Cell Events: 9815 mean_circ: 0.93893
Ran for 214550 frames Cell Events: 9834 mean_circ: 0.938954
Ran for 215050 frames Cell Events: 9846 mean_circ: 0.938971
Ran for 215550 frames Cell Events: 9863 mean_circ: 0.938991
Ran for 216050 frames Cell Events: 9890 mean_circ: 0.939013
Ran for 217050 frames Cell Events: 9940 mean_circ: 0.939039
Ran for 218050 frames Cell Events: 9980 mean_circ: 0.93906



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 6.2655e+008
****************************************
Measurement finished

Mean Circularitiy: 0.939065
Mean Area: 563.962
Mean FL-1 intensity: 3112.02
Mean FL-2 intensity: 4764.01
Mean FL-3 intensity: 0

frames processed: 216857
Total Time: 109345ms
Number of Runs (new bg img) 0
Frames collected: 218548
Skipped frames: 25171
Rescued Frames: 1274856
Samples DAQ: 54637130
Samples written: 109274180
Samples per Channel: 54637090
FPS: 1998.69

Samples per second: 499674
Expected samples/s: 500000

Events: 10000
Events per second: 91.4533
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :304634
# 1 frames :115134
# 2 frames :5002
# 3 frames :1312
# 4 frames :498
# 5 frames :182
# 6 frames :4403
# 7 frames :1591
# 8 frames :1663
# 9 frames :568
# 10 frames :397
# 11 frames :255
# 12 frames :295
# 13 frames :136
# 14 frames :354
# 15 frames :147
# 16 frames :115
# 17 frames :89
# 18 frames :53
# 19 frames :22
# 20 frames :46
# 21 frames :34
# 22 frames :34
# 23 frames :32
# 24 frames :12
# 25 frames :6
# 26 frames :10
# 27 frames :6
# 28 frames :5
# 29 frames :10
# 30 frames :8
# 31 frames :2
# 32 frames :3
# 33 frames :2
# 34 frames :1
# 35 frames :1
# 52 frames :1
# 66 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.2565
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0