Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.29.33 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "711679c1fb26557bd76b5a80e52ebf58",
"name": "M1_20170522_1534_dsRNA_D6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:29:54"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8068 (max 8069) in '8068'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.9827
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.30685e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 33 mean_circ: 0.9432
Ran for 1050 frames Cell Events: 54 mean_circ: 0.941596
Ran for 1550 frames Cell Events: 73 mean_circ: 0.93819
Ran for 2050 frames Cell Events: 94 mean_circ: 0.93684
Ran for 3050 frames Cell Events: 154 mean_circ: 0.93911
Ran for 4050 frames Cell Events: 217 mean_circ: 0.93965
Ran for 4550 frames Cell Events: 234 mean_circ: 0.939759
Ran for 5050 frames Cell Events: 264 mean_circ: 0.939387
Ran for 7050 frames Cell Events: 350 mean_circ: 0.938553
Ran for 7550 frames Cell Events: 367 mean_circ: 0.937877
Ran for 8550 frames Cell Events: 422 mean_circ: 0.937767
Ran for 9050 frames Cell Events: 448 mean_circ: 0.93775
Ran for 9550 frames Cell Events: 474 mean_circ: 0.938034
Ran for 10050 frames Cell Events: 505 mean_circ: 0.93653
Ran for 10550 frames Cell Events: 544 mean_circ: 0.935729
Ran for 11050 frames Cell Events: 575 mean_circ: 0.935381
Ran for 11550 frames Cell Events: 596 mean_circ: 0.935285
Ran for 14050 frames Cell Events: 741 mean_circ: 0.935033
Ran for 14550 frames Cell Events: 758 mean_circ: 0.934912
Ran for 15050 frames Cell Events: 782 mean_circ: 0.935031
Ran for 16550 frames Cell Events: 860 mean_circ: 0.934819
Ran for 17050 frames Cell Events: 880 mean_circ: 0.934944
Ran for 17550 frames Cell Events: 901 mean_circ: 0.935167
Ran for 20550 frames Cell Events: 1053 mean_circ: 0.934451
Ran for 21050 frames Cell Events: 1078 mean_circ: 0.934538
Ran for 24050 frames Cell Events: 1275 mean_circ: 0.933936
Ran for 25050 frames Cell Events: 1331 mean_circ: 0.934205
Ran for 25550 frames Cell Events: 1367 mean_circ: 0.934315
Ran for 26050 frames Cell Events: 1390 mean_circ: 0.934458
Ran for 26550 frames Cell Events: 1418 mean_circ: 0.934423
Ran for 29050 frames Cell Events: 1575 mean_circ: 0.934418
Ran for 29550 frames Cell Events: 1594 mean_circ: 0.934573
Ran for 30050 frames Cell Events: 1626 mean_circ: 0.934558
Ran for 30550 frames Cell Events: 1651 mean_circ: 0.934575
Ran for 31050 frames Cell Events: 1679 mean_circ: 0.934454
Ran for 32050 frames Cell Events: 1745 mean_circ: 0.934367
Ran for 33050 frames Cell Events: 1814 mean_circ: 0.933853
Ran for 34550 frames Cell Events: 1880 mean_circ: 0.933831
Ran for 37050 frames Cell Events: 2038 mean_circ: 0.933759
Ran for 38550 frames Cell Events: 2143 mean_circ: 0.934002
Ran for 40050 frames Cell Events: 2244 mean_circ: 0.933905
Ran for 40550 frames Cell Events: 2266 mean_circ: 0.933832
Ran for 42050 frames Cell Events: 2348 mean_circ: 0.933717
Ran for 42550 frames Cell Events: 2375 mean_circ: 0.933705
Ran for 43550 frames Cell Events: 2434 mean_circ: 0.933783
Ran for 44050 frames Cell Events: 2458 mean_circ: 0.933839
Ran for 44550 frames Cell Events: 2478 mean_circ: 0.933808
Ran for 45050 frames Cell Events: 2503 mean_circ: 0.933771
Ran for 45550 frames Cell Events: 2536 mean_circ: 0.933681
Ran for 46050 frames Cell Events: 2563 mean_circ: 0.933652
Ran for 46550 frames Cell Events: 2584 mean_circ: 0.933727
Ran for 47550 frames Cell Events: 2632 mean_circ: 0.933538
Ran for 48550 frames Cell Events: 2702 mean_circ: 0.933597
Ran for 49550 frames Cell Events: 2761 mean_circ: 0.933682
Ran for 50050 frames Cell Events: 2801 mean_circ: 0.933561
Ran for 51550 frames Cell Events: 2888 mean_circ: 0.93369
Ran for 52050 frames Cell Events: 2910 mean_circ: 0.933698
Ran for 52550 frames Cell Events: 2934 mean_circ: 0.933729
Ran for 53550 frames Cell Events: 2988 mean_circ: 0.933779
Ran for 55550 frames Cell Events: 3106 mean_circ: 0.933724
Ran for 56050 frames Cell Events: 3129 mean_circ: 0.933717
Ran for 57050 frames Cell Events: 3178 mean_circ: 0.933755
Ran for 58050 frames Cell Events: 3216 mean_circ: 0.933747
Ran for 60050 frames Cell Events: 3342 mean_circ: 0.933774
Ran for 61050 frames Cell Events: 3388 mean_circ: 0.933745
Ran for 63550 frames Cell Events: 3526 mean_circ: 0.933747
Ran for 64050 frames Cell Events: 3550 mean_circ: 0.933745
Ran for 64550 frames Cell Events: 3589 mean_circ: 0.933825
Ran for 65050 frames Cell Events: 3616 mean_circ: 0.933863
Ran for 65550 frames Cell Events: 3636 mean_circ: 0.933861
Ran for 67050 frames Cell Events: 3723 mean_circ: 0.933943
Ran for 67550 frames Cell Events: 3747 mean_circ: 0.934011
Ran for 68550 frames Cell Events: 3798 mean_circ: 0.933915
Ran for 69550 frames Cell Events: 3849 mean_circ: 0.933745
Ran for 70050 frames Cell Events: 3862 mean_circ: 0.933771
Ran for 70550 frames Cell Events: 3886 mean_circ: 0.933711
Ran for 71050 frames Cell Events: 3898 mean_circ: 0.933711
Ran for 72050 frames Cell Events: 3944 mean_circ: 0.933716
Ran for 73550 frames Cell Events: 4042 mean_circ: 0.93369
Ran for 74050 frames Cell Events: 4069 mean_circ: 0.933736
Ran for 75050 frames Cell Events: 4123 mean_circ: 0.933831
Ran for 76050 frames Cell Events: 4183 mean_circ: 0.933864
Ran for 77550 frames Cell Events: 4293 mean_circ: 0.933851
Ran for 78050 frames Cell Events: 4317 mean_circ: 0.933778
Ran for 78550 frames Cell Events: 4358 mean_circ: 0.933738
Ran for 79050 frames Cell Events: 4375 mean_circ: 0.933765
Ran for 79550 frames Cell Events: 4389 mean_circ: 0.933691
Ran for 80550 frames Cell Events: 4463 mean_circ: 0.933674
Ran for 81050 frames Cell Events: 4484 mean_circ: 0.933678
Ran for 81550 frames Cell Events: 4508 mean_circ: 0.933589
Ran for 82550 frames Cell Events: 4559 mean_circ: 0.933588
Ran for 83550 frames Cell Events: 4627 mean_circ: 0.933588
Ran for 84550 frames Cell Events: 4698 mean_circ: 0.933312
Ran for 85050 frames Cell Events: 4728 mean_circ: 0.933317
Ran for 85550 frames Cell Events: 4751 mean_circ: 0.933338
Ran for 86550 frames Cell Events: 4794 mean_circ: 0.933291
Ran for 87050 frames Cell Events: 4827 mean_circ: 0.933181
Ran for 91050 frames Cell Events: 5102 mean_circ: 0.933292
Ran for 91550 frames Cell Events: 5131 mean_circ: 0.93331
Ran for 92050 frames Cell Events: 5154 mean_circ: 0.933217
Ran for 94550 frames Cell Events: 5309 mean_circ: 0.933346
Ran for 95050 frames Cell Events: 5333 mean_circ: 0.933411
Ran for 95550 frames Cell Events: 5376 mean_circ: 0.933411
Ran for 96550 frames Cell Events: 5433 mean_circ: 0.933436
Ran for 97050 frames Cell Events: 5461 mean_circ: 0.933515
Ran for 97550 frames Cell Events: 5481 mean_circ: 0.933435
Ran for 98050 frames Cell Events: 5509 mean_circ: 0.933415
Ran for 98550 frames Cell Events: 5549 mean_circ: 0.933368
Ran for 99050 frames Cell Events: 5576 mean_circ: 0.933278
Ran for 100050 frames Cell Events: 5634 mean_circ: 0.933256
Ran for 100550 frames Cell Events: 5655 mean_circ: 0.93324
Ran for 101050 frames Cell Events: 5680 mean_circ: 0.933143
Ran for 101550 frames Cell Events: 5707 mean_circ: 0.933154
Ran for 102050 frames Cell Events: 5728 mean_circ: 0.93317
Ran for 102550 frames Cell Events: 5756 mean_circ: 0.933178
Ran for 103050 frames Cell Events: 5784 mean_circ: 0.933147
Ran for 103550 frames Cell Events: 5810 mean_circ: 0.933136
Ran for 104550 frames Cell Events: 5855 mean_circ: 0.933117
Ran for 105050 frames Cell Events: 5884 mean_circ: 0.933176
Ran for 106050 frames Cell Events: 5939 mean_circ: 0.933249
Ran for 107050 frames Cell Events: 6013 mean_circ: 0.933255
Ran for 109550 frames Cell Events: 6165 mean_circ: 0.933256
Ran for 110550 frames Cell Events: 6226 mean_circ: 0.933211
Ran for 111050 frames Cell Events: 6247 mean_circ: 0.933181
Ran for 111550 frames Cell Events: 6264 mean_circ: 0.933232
Ran for 113550 frames Cell Events: 6416 mean_circ: 0.933197
Ran for 114050 frames Cell Events: 6435 mean_circ: 0.933238
Ran for 114550 frames Cell Events: 6457 mean_circ: 0.933251
Ran for 115550 frames Cell Events: 6507 mean_circ: 0.933304
Ran for 118050 frames Cell Events: 6652 mean_circ: 0.93336
Ran for 120050 frames Cell Events: 6779 mean_circ: 0.933482
Ran for 120550 frames Cell Events: 6801 mean_circ: 0.93346
Ran for 121550 frames Cell Events: 6841 mean_circ: 0.933521
Ran for 122050 frames Cell Events: 6878 mean_circ: 0.933495
Ran for 123550 frames Cell Events: 6988 mean_circ: 0.933322
Ran for 124050 frames Cell Events: 7014 mean_circ: 0.933317
Ran for 124550 frames Cell Events: 7048 mean_circ: 0.933314
Ran for 125050 frames Cell Events: 7081 mean_circ: 0.93328
Ran for 125550 frames Cell Events: 7105 mean_circ: 0.933244
Ran for 127050 frames Cell Events: 7199 mean_circ: 0.933196
Ran for 127550 frames Cell Events: 7215 mean_circ: 0.933253
Ran for 128550 frames Cell Events: 7264 mean_circ: 0.933264
Ran for 129050 frames Cell Events: 7286 mean_circ: 0.933281
Ran for 129550 frames Cell Events: 7309 mean_circ: 0.933316
Ran for 130550 frames Cell Events: 7375 mean_circ: 0.933316
Ran for 131550 frames Cell Events: 7441 mean_circ: 0.933234
Ran for 132050 frames Cell Events: 7467 mean_circ: 0.933259
Ran for 132550 frames Cell Events: 7488 mean_circ: 0.933256
Ran for 133050 frames Cell Events: 7522 mean_circ: 0.933251
Ran for 133550 frames Cell Events: 7555 mean_circ: 0.933234
Ran for 138050 frames Cell Events: 7821 mean_circ: 0.933292
Ran for 138550 frames Cell Events: 7842 mean_circ: 0.933281
Ran for 139050 frames Cell Events: 7870 mean_circ: 0.933207
Ran for 139550 frames Cell Events: 7912 mean_circ: 0.933167
Ran for 140550 frames Cell Events: 7979 mean_circ: 0.933226
Ran for 141050 frames Cell Events: 8010 mean_circ: 0.933259
Ran for 141550 frames Cell Events: 8033 mean_circ: 0.933276



End this measurment..



Stopping time: 6.30756e+008
****************************************
Measurement finished

Mean Circularitiy: 0.933304
Mean Area: 592.108
Mean FL-1 intensity: 3228.37
Mean FL-2 intensity: 4986.56
Mean FL-3 intensity: 0

frames processed: 141842
Total Time: 71181.3ms
Number of Runs (new bg img) 0
Frames collected: 142233
Skipped frames: 42544
Rescued Frames: 2241970
Samples DAQ: 35558201
Samples written: 71116316
Samples per Channel: 35558158
FPS: 1998.18

Samples per second: 499544
Expected samples/s: 500000

Events: 8069
Events per second: 113.358
jumped back (PMT): 9
#Frames read, frequency
# 0 frames :216529
# 1 frames :55474
# 2 frames :3402
# 3 frames :878
# 4 frames :577
# 5 frames :197
# 6 frames :1729
# 7 frames :1280
# 8 frames :866
# 9 frames :592
# 10 frames :994
# 11 frames :297
# 12 frames :248
# 13 frames :129
# 14 frames :175
# 15 frames :201
# 16 frames :180
# 17 frames :117
# 18 frames :90
# 19 frames :44
# 20 frames :36
# 21 frames :50
# 22 frames :52
# 23 frames :42
# 24 frames :38
# 25 frames :21
# 26 frames :28
# 27 frames :19
# 28 frames :29
# 29 frames :16
# 30 frames :9
# 31 frames :16
# 32 frames :8
# 33 frames :12
# 34 frames :13
# 35 frames :7
# 36 frames :4
# 37 frames :10
# 38 frames :6
# 39 frames :3
# 40 frames :2
# 41 frames :3
# 42 frames :2
# 43 frames :1
# 44 frames :4
# 45 frames :1
# 48 frames :1
# 51 frames :3
# 54 frames :2
# 55 frames :1
# 75 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.9827
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0