Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.45.44 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f85e892f0c6eb159bf74a50a843cf2bc",
"name": "M1_20170523_1327_NK1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:46:08"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.743
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.09498e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.942232
Ran for 550 frames Cell Events: 22 mean_circ: 0.940756
Ran for 1550 frames Cell Events: 72 mean_circ: 0.922358
Ran for 2050 frames Cell Events: 98 mean_circ: 0.925306
Ran for 2550 frames Cell Events: 131 mean_circ: 0.927653
Ran for 3050 frames Cell Events: 157 mean_circ: 0.92732
Ran for 4050 frames Cell Events: 201 mean_circ: 0.928218
Ran for 4550 frames Cell Events: 218 mean_circ: 0.927197
Ran for 5050 frames Cell Events: 238 mean_circ: 0.927304
Ran for 5550 frames Cell Events: 265 mean_circ: 0.927214
Ran for 6050 frames Cell Events: 289 mean_circ: 0.928198
Ran for 6550 frames Cell Events: 311 mean_circ: 0.928743
Ran for 7050 frames Cell Events: 334 mean_circ: 0.929042
Ran for 7550 frames Cell Events: 358 mean_circ: 0.928976
Ran for 8050 frames Cell Events: 378 mean_circ: 0.92901
Ran for 11050 frames Cell Events: 533 mean_circ: 0.929469
Ran for 14050 frames Cell Events: 701 mean_circ: 0.929578
Ran for 14550 frames Cell Events: 740 mean_circ: 0.929697
Ran for 16050 frames Cell Events: 811 mean_circ: 0.92942
Ran for 17050 frames Cell Events: 860 mean_circ: 0.92916
Ran for 17550 frames Cell Events: 880 mean_circ: 0.929334
Ran for 19050 frames Cell Events: 973 mean_circ: 0.928924
Ran for 19550 frames Cell Events: 1012 mean_circ: 0.928529
Ran for 20050 frames Cell Events: 1042 mean_circ: 0.928441
Ran for 22550 frames Cell Events: 1183 mean_circ: 0.929603
Ran for 25050 frames Cell Events: 1341 mean_circ: 0.929638
Ran for 25550 frames Cell Events: 1366 mean_circ: 0.929607
Ran for 28050 frames Cell Events: 1511 mean_circ: 0.929474
Ran for 29550 frames Cell Events: 1600 mean_circ: 0.92862
Ran for 30050 frames Cell Events: 1630 mean_circ: 0.92861
Ran for 31050 frames Cell Events: 1685 mean_circ: 0.928656
Ran for 31550 frames Cell Events: 1702 mean_circ: 0.92874
Ran for 33050 frames Cell Events: 1776 mean_circ: 0.928778
Ran for 34550 frames Cell Events: 1848 mean_circ: 0.928504
Ran for 35550 frames Cell Events: 1914 mean_circ: 0.928309
Ran for 36550 frames Cell Events: 1968 mean_circ: 0.928205
Ran for 37050 frames Cell Events: 1998 mean_circ: 0.928164
Ran for 37550 frames Cell Events: 2024 mean_circ: 0.92818
Ran for 38050 frames Cell Events: 2045 mean_circ: 0.928154
Ran for 38550 frames Cell Events: 2070 mean_circ: 0.928117
Ran for 39050 frames Cell Events: 2098 mean_circ: 0.928335
Ran for 40050 frames Cell Events: 2144 mean_circ: 0.928312
Ran for 40550 frames Cell Events: 2166 mean_circ: 0.928364
Ran for 41550 frames Cell Events: 2225 mean_circ: 0.928819
Ran for 43050 frames Cell Events: 2309 mean_circ: 0.929115
Ran for 43550 frames Cell Events: 2338 mean_circ: 0.929029
Ran for 47550 frames Cell Events: 2537 mean_circ: 0.928774
Ran for 48050 frames Cell Events: 2563 mean_circ: 0.928723
Ran for 49550 frames Cell Events: 2646 mean_circ: 0.928863
Ran for 52050 frames Cell Events: 2773 mean_circ: 0.928771
Ran for 54050 frames Cell Events: 2891 mean_circ: 0.928772
Ran for 54550 frames Cell Events: 2916 mean_circ: 0.928918
Ran for 55050 frames Cell Events: 2940 mean_circ: 0.928949
Ran for 55550 frames Cell Events: 2966 mean_circ: 0.928949
Ran for 56550 frames Cell Events: 3006 mean_circ: 0.928997
Ran for 57550 frames Cell Events: 3067 mean_circ: 0.929087
Ran for 59550 frames Cell Events: 3169 mean_circ: 0.928989
Ran for 60050 frames Cell Events: 3187 mean_circ: 0.928916
Ran for 60550 frames Cell Events: 3212 mean_circ: 0.928943
Ran for 61050 frames Cell Events: 3236 mean_circ: 0.928973
Ran for 61550 frames Cell Events: 3266 mean_circ: 0.928982
Ran for 62050 frames Cell Events: 3290 mean_circ: 0.929013
Ran for 62550 frames Cell Events: 3313 mean_circ: 0.92895
Ran for 63050 frames Cell Events: 3351 mean_circ: 0.928977
Ran for 63550 frames Cell Events: 3379 mean_circ: 0.929011
Ran for 67050 frames Cell Events: 3592 mean_circ: 0.929182
Ran for 67550 frames Cell Events: 3615 mean_circ: 0.929287
Ran for 68550 frames Cell Events: 3661 mean_circ: 0.929242
Ran for 69550 frames Cell Events: 3723 mean_circ: 0.929251
Ran for 71050 frames Cell Events: 3804 mean_circ: 0.929293
Ran for 72050 frames Cell Events: 3865 mean_circ: 0.929401
Ran for 72550 frames Cell Events: 3896 mean_circ: 0.929207
Ran for 73050 frames Cell Events: 3929 mean_circ: 0.929216
Ran for 74050 frames Cell Events: 3984 mean_circ: 0.929271
Ran for 76050 frames Cell Events: 4085 mean_circ: 0.929119
Ran for 76550 frames Cell Events: 4113 mean_circ: 0.929162
Ran for 78050 frames Cell Events: 4192 mean_circ: 0.929364
Ran for 78550 frames Cell Events: 4223 mean_circ: 0.929344
Ran for 79050 frames Cell Events: 4249 mean_circ: 0.929163
Ran for 79550 frames Cell Events: 4283 mean_circ: 0.929188
Ran for 80050 frames Cell Events: 4310 mean_circ: 0.929176
Ran for 80550 frames Cell Events: 4348 mean_circ: 0.929102
Ran for 82550 frames Cell Events: 4470 mean_circ: 0.929014
Ran for 84050 frames Cell Events: 4552 mean_circ: 0.928977
Ran for 85050 frames Cell Events: 4615 mean_circ: 0.92902
Ran for 86050 frames Cell Events: 4665 mean_circ: 0.929052
Ran for 86550 frames Cell Events: 4694 mean_circ: 0.929088
Ran for 87050 frames Cell Events: 4716 mean_circ: 0.92902
Ran for 88050 frames Cell Events: 4774 mean_circ: 0.928975
Ran for 89050 frames Cell Events: 4820 mean_circ: 0.928914
Ran for 89550 frames Cell Events: 4843 mean_circ: 0.928886
Ran for 90050 frames Cell Events: 4865 mean_circ: 0.928935
Ran for 90550 frames Cell Events: 4899 mean_circ: 0.928937
Ran for 91050 frames Cell Events: 4930 mean_circ: 0.928886
Ran for 91550 frames Cell Events: 4960 mean_circ: 0.928894
Ran for 93050 frames Cell Events: 5055 mean_circ: 0.928914
Ran for 93550 frames Cell Events: 5079 mean_circ: 0.928962
Ran for 94050 frames Cell Events: 5111 mean_circ: 0.929041
Ran for 96050 frames Cell Events: 5233 mean_circ: 0.929121
Ran for 98550 frames Cell Events: 5383 mean_circ: 0.929036
Ran for 100050 frames Cell Events: 5459 mean_circ: 0.92894
Ran for 100550 frames Cell Events: 5489 mean_circ: 0.928934
Ran for 101050 frames Cell Events: 5528 mean_circ: 0.928934
Ran for 101550 frames Cell Events: 5554 mean_circ: 0.928874
Ran for 102050 frames Cell Events: 5579 mean_circ: 0.928798
Ran for 104550 frames Cell Events: 5699 mean_circ: 0.928916
Ran for 107550 frames Cell Events: 5872 mean_circ: 0.928979
Ran for 110050 frames Cell Events: 6017 mean_circ: 0.928896
Ran for 110550 frames Cell Events: 6029 mean_circ: 0.928881
Ran for 112050 frames Cell Events: 6098 mean_circ: 0.92884
Ran for 113050 frames Cell Events: 6148 mean_circ: 0.928909
Ran for 113550 frames Cell Events: 6165 mean_circ: 0.92892
Ran for 114050 frames Cell Events: 6189 mean_circ: 0.928917
Ran for 115050 frames Cell Events: 6240 mean_circ: 0.928879
Ran for 115550 frames Cell Events: 6279 mean_circ: 0.92893
Ran for 116550 frames Cell Events: 6320 mean_circ: 0.92882
Ran for 117050 frames Cell Events: 6340 mean_circ: 0.928811
Ran for 117550 frames Cell Events: 6371 mean_circ: 0.928777
Ran for 118050 frames Cell Events: 6393 mean_circ: 0.928827
Ran for 118550 frames Cell Events: 6422 mean_circ: 0.928845
Ran for 119050 frames Cell Events: 6453 mean_circ: 0.928847
Ran for 119550 frames Cell Events: 6474 mean_circ: 0.928866
Ran for 120050 frames Cell Events: 6503 mean_circ: 0.928871
Ran for 123550 frames Cell Events: 6663 mean_circ: 0.928753
Ran for 124050 frames Cell Events: 6685 mean_circ: 0.928782
Ran for 124550 frames Cell Events: 6704 mean_circ: 0.928787
Ran for 125050 frames Cell Events: 6727 mean_circ: 0.928797
Ran for 125550 frames Cell Events: 6747 mean_circ: 0.928814
Ran for 127050 frames Cell Events: 6843 mean_circ: 0.928862
Ran for 127550 frames Cell Events: 6866 mean_circ: 0.928892
Ran for 128050 frames Cell Events: 6906 mean_circ: 0.928862
Ran for 130550 frames Cell Events: 7052 mean_circ: 0.92883
Ran for 131550 frames Cell Events: 7115 mean_circ: 0.928823
Ran for 132050 frames Cell Events: 7137 mean_circ: 0.92874
Ran for 133550 frames Cell Events: 7233 mean_circ: 0.928794
Ran for 136050 frames Cell Events: 7397 mean_circ: 0.928786
Ran for 136550 frames Cell Events: 7425 mean_circ: 0.928824
Ran for 137050 frames Cell Events: 7447 mean_circ: 0.928807
Ran for 137550 frames Cell Events: 7476 mean_circ: 0.928828
Ran for 138550 frames Cell Events: 7531 mean_circ: 0.928861
Ran for 139550 frames Cell Events: 7614 mean_circ: 0.928751
Ran for 140550 frames Cell Events: 7649 mean_circ: 0.92874
Ran for 141050 frames Cell Events: 7688 mean_circ: 0.928751
Ran for 141550 frames Cell Events: 7719 mean_circ: 0.928761
Ran for 143550 frames Cell Events: 7867 mean_circ: 0.928873
Ran for 145050 frames Cell Events: 7975 mean_circ: 0.92887
Ran for 148550 frames Cell Events: 8193 mean_circ: 0.928889
Ran for 149550 frames Cell Events: 8242 mean_circ: 0.928913
Ran for 151050 frames Cell Events: 8309 mean_circ: 0.928942
Ran for 151550 frames Cell Events: 8326 mean_circ: 0.928935
Ran for 152050 frames Cell Events: 8358 mean_circ: 0.928944
Ran for 152550 frames Cell Events: 8383 mean_circ: 0.928957
Ran for 153550 frames Cell Events: 8430 mean_circ: 0.929014
Ran for 155050 frames Cell Events: 8521 mean_circ: 0.928969
Ran for 156550 frames Cell Events: 8625 mean_circ: 0.928847
Ran for 158050 frames Cell Events: 8723 mean_circ: 0.928889
Ran for 158550 frames Cell Events: 8754 mean_circ: 0.928885
Ran for 161050 frames Cell Events: 8877 mean_circ: 0.928834
Ran for 162050 frames Cell Events: 8934 mean_circ: 0.928817
Ran for 162550 frames Cell Events: 8959 mean_circ: 0.928843
Ran for 163050 frames Cell Events: 8988 mean_circ: 0.928865
Ran for 163550 frames Cell Events: 9004 mean_circ: 0.928789
Ran for 164050 frames Cell Events: 9041 mean_circ: 0.928736
Ran for 166550 frames Cell Events: 9188 mean_circ: 0.928767
Ran for 167050 frames Cell Events: 9221 mean_circ: 0.928768
Ran for 168050 frames Cell Events: 9265 mean_circ: 0.928769
Ran for 170050 frames Cell Events: 9348 mean_circ: 0.928765



End this measurment..



Ran for 174550 frames Cell Events: 9617 mean_circ: 0.928638
Stopping time: 7.09585e+008
****************************************
Measurement finished

Mean Circularitiy: 0.928637
Mean Area: 635.07
Mean FL-1 intensity: 3197.6
Mean FL-2 intensity: 4660.66
Mean FL-3 intensity: 0

frames processed: 170503
Total Time: 87348.9ms
Number of Runs (new bg img) 0
Frames collected: 174550
Skipped frames: 6964
Rescued Frames: 181340
Samples DAQ: 43638844
Samples written: 87274780
Samples per Channel: 43637390
FPS: 1998.31

Samples per second: 499576
Expected samples/s: 500000

Events: 9617
Events per second: 110.099
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :268823
# 1 frames :65783
# 2 frames :3407
# 3 frames :1166
# 4 frames :626
# 5 frames :300
# 6 frames :2545
# 7 frames :1250
# 8 frames :1169
# 9 frames :772
# 10 frames :779
# 11 frames :357
# 12 frames :285
# 13 frames :188
# 14 frames :246
# 15 frames :191
# 16 frames :190
# 17 frames :117
# 18 frames :101
# 19 frames :78
# 20 frames :90
# 21 frames :78
# 22 frames :49
# 23 frames :48
# 24 frames :42
# 25 frames :50
# 26 frames :27
# 27 frames :20
# 28 frames :26
# 29 frames :21
# 30 frames :24
# 31 frames :23
# 32 frames :17
# 33 frames :18
# 34 frames :10
# 35 frames :11
# 36 frames :9
# 37 frames :8
# 38 frames :13
# 39 frames :10
# 40 frames :4
# 41 frames :9
# 42 frames :6
# 43 frames :8
# 44 frames :9
# 45 frames :3
# 46 frames :4
# 47 frames :8
# 48 frames :2
# 49 frames :4
# 50 frames :2
# 51 frames :2
# 52 frames :3
# 54 frames :1
# 55 frames :3
# 56 frames :3
# 57 frames :1
# 58 frames :3
# 59 frames :3
# 60 frames :2
# 61 frames :2
# 62 frames :1
# 63 frames :2
# 64 frames :1
# 65 frames :2
# 66 frames :1
# 67 frames :1
# 68 frames :3
# 69 frames :1
# 70 frames :1
# 73 frames :1
# 74 frames :1
# 75 frames :1
# 76 frames :1
# 78 frames :1
# 79 frames :1
# 80 frames :1
# 91 frames :1
# 98 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.743
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0