Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.49.10 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d8d7165b7ec0dc00cdc23fad5550a9cd",
"name": "M1_20170523_1413_dsRNA_G8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:49:30"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8119 (max 8121) in '8119'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9978
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.12228e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.758951
Ran for 2050 frames Cell Events: 128 mean_circ: 0.931047
Ran for 2550 frames Cell Events: 135 mean_circ: 0.933505
Ran for 3050 frames Cell Events: 163 mean_circ: 0.936375
Ran for 4050 frames Cell Events: 208 mean_circ: 0.938693
Ran for 5050 frames Cell Events: 260 mean_circ: 0.940653
Ran for 5550 frames Cell Events: 280 mean_circ: 0.94105
Ran for 7550 frames Cell Events: 392 mean_circ: 0.939716
Ran for 8550 frames Cell Events: 429 mean_circ: 0.94068
Ran for 11050 frames Cell Events: 553 mean_circ: 0.940522
Ran for 11550 frames Cell Events: 580 mean_circ: 0.940898
Ran for 13550 frames Cell Events: 670 mean_circ: 0.941156
Ran for 15550 frames Cell Events: 803 mean_circ: 0.940992
Ran for 17550 frames Cell Events: 905 mean_circ: 0.942007
Ran for 18050 frames Cell Events: 933 mean_circ: 0.942004
Ran for 19550 frames Cell Events: 1014 mean_circ: 0.94214
Ran for 20050 frames Cell Events: 1042 mean_circ: 0.94218
Ran for 20550 frames Cell Events: 1078 mean_circ: 0.942116
Ran for 21050 frames Cell Events: 1117 mean_circ: 0.942171
Ran for 22550 frames Cell Events: 1186 mean_circ: 0.941914
Ran for 23550 frames Cell Events: 1248 mean_circ: 0.941747
Ran for 24550 frames Cell Events: 1289 mean_circ: 0.941646
Ran for 25550 frames Cell Events: 1349 mean_circ: 0.941719
Ran for 26050 frames Cell Events: 1365 mean_circ: 0.941829
Ran for 28050 frames Cell Events: 1497 mean_circ: 0.942278
Ran for 29050 frames Cell Events: 1549 mean_circ: 0.942089
Ran for 30050 frames Cell Events: 1610 mean_circ: 0.942479
Ran for 30550 frames Cell Events: 1624 mean_circ: 0.942427
Ran for 34550 frames Cell Events: 1867 mean_circ: 0.941882
Ran for 37550 frames Cell Events: 2101 mean_circ: 0.941969
Ran for 38550 frames Cell Events: 2141 mean_circ: 0.941971
Ran for 39050 frames Cell Events: 2169 mean_circ: 0.94216
Ran for 39550 frames Cell Events: 2205 mean_circ: 0.942291
Ran for 40050 frames Cell Events: 2231 mean_circ: 0.94234
Ran for 42050 frames Cell Events: 2372 mean_circ: 0.942061
Ran for 43050 frames Cell Events: 2444 mean_circ: 0.942338
Ran for 44550 frames Cell Events: 2515 mean_circ: 0.942041
Ran for 45050 frames Cell Events: 2540 mean_circ: 0.941844
Ran for 45550 frames Cell Events: 2569 mean_circ: 0.941828
Ran for 46050 frames Cell Events: 2586 mean_circ: 0.94185
Ran for 47050 frames Cell Events: 2640 mean_circ: 0.941898
Ran for 48550 frames Cell Events: 2709 mean_circ: 0.941923
Ran for 49050 frames Cell Events: 2733 mean_circ: 0.941799
Ran for 50550 frames Cell Events: 2823 mean_circ: 0.941976
Ran for 52050 frames Cell Events: 2913 mean_circ: 0.941556
Ran for 52550 frames Cell Events: 2935 mean_circ: 0.941713
Ran for 53050 frames Cell Events: 2960 mean_circ: 0.941721
Ran for 55050 frames Cell Events: 3091 mean_circ: 0.942007
Ran for 55550 frames Cell Events: 3121 mean_circ: 0.942051
Ran for 56550 frames Cell Events: 3169 mean_circ: 0.942199
Ran for 57550 frames Cell Events: 3215 mean_circ: 0.942306
Ran for 58550 frames Cell Events: 3279 mean_circ: 0.942424
Ran for 59550 frames Cell Events: 3326 mean_circ: 0.942542
Ran for 60050 frames Cell Events: 3355 mean_circ: 0.942543
Ran for 61550 frames Cell Events: 3426 mean_circ: 0.942614
Ran for 62050 frames Cell Events: 3453 mean_circ: 0.942561
Ran for 64050 frames Cell Events: 3564 mean_circ: 0.942497
Ran for 65050 frames Cell Events: 3605 mean_circ: 0.94246
Ran for 65550 frames Cell Events: 3624 mean_circ: 0.942511
Ran for 67550 frames Cell Events: 3744 mean_circ: 0.9425
Ran for 68550 frames Cell Events: 3802 mean_circ: 0.942617
Ran for 69050 frames Cell Events: 3830 mean_circ: 0.942569
Ran for 72050 frames Cell Events: 3998 mean_circ: 0.942566
Ran for 73050 frames Cell Events: 4057 mean_circ: 0.942724
Ran for 73550 frames Cell Events: 4095 mean_circ: 0.942696
Ran for 74550 frames Cell Events: 4145 mean_circ: 0.942656
Ran for 75050 frames Cell Events: 4168 mean_circ: 0.942612
Ran for 75550 frames Cell Events: 4195 mean_circ: 0.94265
Ran for 78050 frames Cell Events: 4313 mean_circ: 0.942731
Ran for 78550 frames Cell Events: 4339 mean_circ: 0.942746
Ran for 79550 frames Cell Events: 4400 mean_circ: 0.942671
Ran for 80050 frames Cell Events: 4423 mean_circ: 0.942682
Ran for 80550 frames Cell Events: 4446 mean_circ: 0.942682
Ran for 83050 frames Cell Events: 4610 mean_circ: 0.942684
Ran for 83550 frames Cell Events: 4629 mean_circ: 0.942647
Ran for 84050 frames Cell Events: 4655 mean_circ: 0.942645
Ran for 85050 frames Cell Events: 4718 mean_circ: 0.942672
Ran for 85550 frames Cell Events: 4758 mean_circ: 0.942672
Ran for 86550 frames Cell Events: 4814 mean_circ: 0.942705
Ran for 87050 frames Cell Events: 4852 mean_circ: 0.942693
Ran for 88550 frames Cell Events: 4961 mean_circ: 0.9426
Ran for 89550 frames Cell Events: 5006 mean_circ: 0.942622
Ran for 90050 frames Cell Events: 5030 mean_circ: 0.942652
Ran for 91050 frames Cell Events: 5085 mean_circ: 0.942646
Ran for 92550 frames Cell Events: 5150 mean_circ: 0.942629
Ran for 93550 frames Cell Events: 5199 mean_circ: 0.942632
Ran for 94050 frames Cell Events: 5218 mean_circ: 0.942541
Ran for 97550 frames Cell Events: 5481 mean_circ: 0.94261
Ran for 98050 frames Cell Events: 5500 mean_circ: 0.942614
Ran for 98550 frames Cell Events: 5520 mean_circ: 0.942548
Ran for 100550 frames Cell Events: 5608 mean_circ: 0.942585
Ran for 102050 frames Cell Events: 5698 mean_circ: 0.942571
Ran for 102550 frames Cell Events: 5725 mean_circ: 0.942585
Ran for 103050 frames Cell Events: 5750 mean_circ: 0.94265
Ran for 103550 frames Cell Events: 5775 mean_circ: 0.942696
Ran for 104050 frames Cell Events: 5794 mean_circ: 0.942631
Ran for 105550 frames Cell Events: 5859 mean_circ: 0.942677
Ran for 106050 frames Cell Events: 5886 mean_circ: 0.942682
Ran for 106550 frames Cell Events: 5910 mean_circ: 0.94268
Ran for 108050 frames Cell Events: 6003 mean_circ: 0.942684
Ran for 108550 frames Cell Events: 6033 mean_circ: 0.942676
Ran for 109050 frames Cell Events: 6064 mean_circ: 0.942672
Ran for 109550 frames Cell Events: 6087 mean_circ: 0.942648
Ran for 110550 frames Cell Events: 6138 mean_circ: 0.942616
Ran for 112050 frames Cell Events: 6226 mean_circ: 0.942574
Ran for 112550 frames Cell Events: 6248 mean_circ: 0.942592
Ran for 113050 frames Cell Events: 6265 mean_circ: 0.942572
Ran for 114050 frames Cell Events: 6319 mean_circ: 0.942555
Ran for 114550 frames Cell Events: 6352 mean_circ: 0.942541
Ran for 115050 frames Cell Events: 6366 mean_circ: 0.942549
Ran for 115550 frames Cell Events: 6397 mean_circ: 0.942529
Ran for 116050 frames Cell Events: 6423 mean_circ: 0.94245
Ran for 119550 frames Cell Events: 6605 mean_circ: 0.942557
Ran for 120550 frames Cell Events: 6642 mean_circ: 0.942621
Ran for 121050 frames Cell Events: 6654 mean_circ: 0.942624
Ran for 122050 frames Cell Events: 6724 mean_circ: 0.942547
Ran for 124050 frames Cell Events: 6814 mean_circ: 0.94261
Ran for 124550 frames Cell Events: 6846 mean_circ: 0.942656
Ran for 125050 frames Cell Events: 6875 mean_circ: 0.942646
Ran for 125550 frames Cell Events: 6893 mean_circ: 0.942653
Ran for 126050 frames Cell Events: 6916 mean_circ: 0.942684
Ran for 126550 frames Cell Events: 6941 mean_circ: 0.942697
Ran for 127050 frames Cell Events: 6959 mean_circ: 0.942649
Ran for 127550 frames Cell Events: 6981 mean_circ: 0.942674
Ran for 128550 frames Cell Events: 7020 mean_circ: 0.942719
Ran for 129050 frames Cell Events: 7033 mean_circ: 0.942744
Ran for 129550 frames Cell Events: 7063 mean_circ: 0.942782
Ran for 130550 frames Cell Events: 7124 mean_circ: 0.942732
Ran for 131050 frames Cell Events: 7141 mean_circ: 0.942749
Ran for 131550 frames Cell Events: 7170 mean_circ: 0.942788
Ran for 132050 frames Cell Events: 7196 mean_circ: 0.942795
Ran for 132550 frames Cell Events: 7232 mean_circ: 0.942846
Ran for 133550 frames Cell Events: 7283 mean_circ: 0.94282
Ran for 134050 frames Cell Events: 7298 mean_circ: 0.942829
Ran for 134550 frames Cell Events: 7316 mean_circ: 0.942862
Ran for 135050 frames Cell Events: 7336 mean_circ: 0.94286
Ran for 136050 frames Cell Events: 7375 mean_circ: 0.942859
Ran for 137050 frames Cell Events: 7433 mean_circ: 0.942817
Ran for 137550 frames Cell Events: 7467 mean_circ: 0.942804
Ran for 140050 frames Cell Events: 7608 mean_circ: 0.942752
Ran for 141550 frames Cell Events: 7697 mean_circ: 0.942725
Ran for 142050 frames Cell Events: 7735 mean_circ: 0.942765
Ran for 145550 frames Cell Events: 7944 mean_circ: 0.942671
Ran for 146050 frames Cell Events: 7968 mean_circ: 0.942684
Ran for 146550 frames Cell Events: 8002 mean_circ: 0.942684
Ran for 147050 frames Cell Events: 8025 mean_circ: 0.942663
Ran for 147550 frames Cell Events: 8050 mean_circ: 0.94269
Ran for 148050 frames Cell Events: 8080 mean_circ: 0.942689



End this measurment..



Stopping time: 7.12302e+008
****************************************
Measurement finished

Mean Circularitiy: 0.942733
Mean Area: 629.715
Mean FL-1 intensity: 3563.65
Mean FL-2 intensity: 4529.21
Mean FL-3 intensity: 0

frames processed: 146338
Total Time: 74411.3ms
Number of Runs (new bg img) 0
Frames collected: 148664
Skipped frames: 21460
Rescued Frames: 788224
Samples DAQ: 37168969
Samples written: 74332148
Samples per Channel: 37166074
FPS: 1997.87

Samples per second: 499468
Expected samples/s: 500000

Events: 8121
Events per second: 109.137
jumped back (PMT): 3136
#Frames read, frequency
# 0 frames :230309
# 1 frames :54707
# 2 frames :2743
# 3 frames :1017
# 4 frames :573
# 5 frames :295
# 6 frames :1233
# 7 frames :1207
# 8 frames :833
# 9 frames :553
# 10 frames :672
# 11 frames :454
# 12 frames :251
# 13 frames :193
# 14 frames :226
# 15 frames :175
# 16 frames :174
# 17 frames :144
# 18 frames :110
# 19 frames :93
# 20 frames :85
# 21 frames :78
# 22 frames :65
# 23 frames :48
# 24 frames :53
# 25 frames :36
# 26 frames :40
# 27 frames :45
# 28 frames :27
# 29 frames :29
# 30 frames :24
# 31 frames :24
# 32 frames :18
# 33 frames :15
# 34 frames :12
# 35 frames :12
# 36 frames :10
# 37 frames :13
# 38 frames :8
# 39 frames :9
# 40 frames :12
# 41 frames :10
# 42 frames :6
# 43 frames :6
# 44 frames :10
# 45 frames :1
# 46 frames :7
# 47 frames :4
# 48 frames :2
# 49 frames :10
# 50 frames :2
# 51 frames :5
# 52 frames :2
# 53 frames :3
# 54 frames :2
# 55 frames :5
# 56 frames :2
# 57 frames :2
# 58 frames :1
# 60 frames :3
# 61 frames :1
# 62 frames :1
# 63 frames :1
# 65 frames :1
# 66 frames :2
# 67 frames :2
# 68 frames :1
# 70 frames :1
# 72 frames :2
# 74 frames :1
# 88 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9978
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0