Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.49.36 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2f88e0b74e7bcc13be26eb8321c24446",
"name": "M1_20170523_1415_dsRNA_G8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:49:56"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8120 (max 8121) in '8120'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9973
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.1238e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 90 mean_circ: 0.927071
Ran for 2050 frames Cell Events: 124 mean_circ: 0.925719
Ran for 3050 frames Cell Events: 176 mean_circ: 0.927038
Ran for 3550 frames Cell Events: 210 mean_circ: 0.925175
Ran for 4050 frames Cell Events: 261 mean_circ: 0.926041
Ran for 4550 frames Cell Events: 291 mean_circ: 0.92758
Ran for 7550 frames Cell Events: 473 mean_circ: 0.930334
Ran for 9550 frames Cell Events: 618 mean_circ: 0.932422
Ran for 10050 frames Cell Events: 641 mean_circ: 0.932474
Ran for 10550 frames Cell Events: 677 mean_circ: 0.932513
Ran for 11050 frames Cell Events: 700 mean_circ: 0.932396
Ran for 11550 frames Cell Events: 730 mean_circ: 0.932382
Ran for 12050 frames Cell Events: 749 mean_circ: 0.932182
Ran for 14050 frames Cell Events: 876 mean_circ: 0.93269
Ran for 17550 frames Cell Events: 1078 mean_circ: 0.932601
Ran for 18050 frames Cell Events: 1097 mean_circ: 0.932679
Ran for 19050 frames Cell Events: 1148 mean_circ: 0.93314
Ran for 19550 frames Cell Events: 1174 mean_circ: 0.932767
Ran for 21050 frames Cell Events: 1272 mean_circ: 0.932558
Ran for 22550 frames Cell Events: 1318 mean_circ: 0.932186
Ran for 23050 frames Cell Events: 1351 mean_circ: 0.932142
Ran for 25550 frames Cell Events: 1532 mean_circ: 0.931815
Ran for 26550 frames Cell Events: 1573 mean_circ: 0.93164
Ran for 27050 frames Cell Events: 1601 mean_circ: 0.932008
Ran for 27550 frames Cell Events: 1626 mean_circ: 0.932105
Ran for 28550 frames Cell Events: 1712 mean_circ: 0.932456
Ran for 29050 frames Cell Events: 1742 mean_circ: 0.932356
Ran for 31550 frames Cell Events: 1902 mean_circ: 0.932331
Ran for 32050 frames Cell Events: 1934 mean_circ: 0.932476
Ran for 32550 frames Cell Events: 1959 mean_circ: 0.932525
Ran for 33550 frames Cell Events: 2002 mean_circ: 0.932394
Ran for 34050 frames Cell Events: 2049 mean_circ: 0.932292
Ran for 34550 frames Cell Events: 2090 mean_circ: 0.932121
Ran for 36050 frames Cell Events: 2179 mean_circ: 0.932504
Ran for 36550 frames Cell Events: 2211 mean_circ: 0.932453
Ran for 37050 frames Cell Events: 2232 mean_circ: 0.932223
Ran for 37550 frames Cell Events: 2253 mean_circ: 0.932122
Ran for 38550 frames Cell Events: 2321 mean_circ: 0.931804
Ran for 39050 frames Cell Events: 2350 mean_circ: 0.931644
Ran for 39550 frames Cell Events: 2377 mean_circ: 0.931546
Ran for 40050 frames Cell Events: 2400 mean_circ: 0.931554
Ran for 40550 frames Cell Events: 2423 mean_circ: 0.931701
Ran for 42050 frames Cell Events: 2528 mean_circ: 0.931612
Ran for 42550 frames Cell Events: 2557 mean_circ: 0.931378
Ran for 43050 frames Cell Events: 2590 mean_circ: 0.93138
Ran for 43550 frames Cell Events: 2607 mean_circ: 0.931416
Ran for 44050 frames Cell Events: 2626 mean_circ: 0.931262
Ran for 44550 frames Cell Events: 2641 mean_circ: 0.931363
Ran for 45050 frames Cell Events: 2661 mean_circ: 0.93129
Ran for 45550 frames Cell Events: 2697 mean_circ: 0.931409
Ran for 46050 frames Cell Events: 2716 mean_circ: 0.931511
Ran for 46550 frames Cell Events: 2743 mean_circ: 0.931498
Ran for 47050 frames Cell Events: 2764 mean_circ: 0.931356
Ran for 47550 frames Cell Events: 2796 mean_circ: 0.93142
Ran for 48050 frames Cell Events: 2835 mean_circ: 0.931383
Ran for 49050 frames Cell Events: 2894 mean_circ: 0.931548
Ran for 50550 frames Cell Events: 2976 mean_circ: 0.931441
Ran for 51050 frames Cell Events: 3015 mean_circ: 0.931483
Ran for 51550 frames Cell Events: 3043 mean_circ: 0.931436
Ran for 52550 frames Cell Events: 3096 mean_circ: 0.931368
Ran for 53050 frames Cell Events: 3138 mean_circ: 0.931376
Ran for 54050 frames Cell Events: 3197 mean_circ: 0.931384
Ran for 55550 frames Cell Events: 3317 mean_circ: 0.93117
Ran for 56550 frames Cell Events: 3386 mean_circ: 0.931293
Ran for 59050 frames Cell Events: 3524 mean_circ: 0.931221
Ran for 60050 frames Cell Events: 3591 mean_circ: 0.931314
Ran for 60550 frames Cell Events: 3621 mean_circ: 0.931453
Ran for 61550 frames Cell Events: 3693 mean_circ: 0.931558
Ran for 62550 frames Cell Events: 3758 mean_circ: 0.931426
Ran for 63550 frames Cell Events: 3837 mean_circ: 0.931395
Ran for 64050 frames Cell Events: 3854 mean_circ: 0.931162
Ran for 64550 frames Cell Events: 3891 mean_circ: 0.931191
Ran for 65050 frames Cell Events: 3922 mean_circ: 0.931188
Ran for 65550 frames Cell Events: 3946 mean_circ: 0.931275
Ran for 66050 frames Cell Events: 3978 mean_circ: 0.931342
Ran for 70050 frames Cell Events: 4228 mean_circ: 0.931187
Ran for 72550 frames Cell Events: 4406 mean_circ: 0.931078
Ran for 73050 frames Cell Events: 4434 mean_circ: 0.931101
Ran for 74050 frames Cell Events: 4500 mean_circ: 0.931013
Ran for 75050 frames Cell Events: 4549 mean_circ: 0.930973
Ran for 76050 frames Cell Events: 4609 mean_circ: 0.93111
Ran for 77550 frames Cell Events: 4710 mean_circ: 0.930979
Ran for 79050 frames Cell Events: 4825 mean_circ: 0.931142
Ran for 80050 frames Cell Events: 4883 mean_circ: 0.930953
Ran for 81050 frames Cell Events: 4941 mean_circ: 0.931077
Ran for 82050 frames Cell Events: 5005 mean_circ: 0.931058
Ran for 82550 frames Cell Events: 5030 mean_circ: 0.931083
Ran for 84550 frames Cell Events: 5144 mean_circ: 0.931114
Ran for 85550 frames Cell Events: 5196 mean_circ: 0.931126
Ran for 86050 frames Cell Events: 5217 mean_circ: 0.931157
Ran for 88550 frames Cell Events: 5366 mean_circ: 0.931164
Ran for 89050 frames Cell Events: 5386 mean_circ: 0.931121
Ran for 89550 frames Cell Events: 5408 mean_circ: 0.931168
Ran for 90050 frames Cell Events: 5432 mean_circ: 0.931121
Ran for 92050 frames Cell Events: 5518 mean_circ: 0.931262
Ran for 93050 frames Cell Events: 5561 mean_circ: 0.931246
Ran for 93550 frames Cell Events: 5590 mean_circ: 0.931234
Ran for 94050 frames Cell Events: 5624 mean_circ: 0.931308
Ran for 94550 frames Cell Events: 5664 mean_circ: 0.931277
Ran for 96550 frames Cell Events: 5788 mean_circ: 0.931239
Ran for 97050 frames Cell Events: 5806 mean_circ: 0.931283
Ran for 97550 frames Cell Events: 5832 mean_circ: 0.931355
Ran for 99550 frames Cell Events: 5962 mean_circ: 0.931383
Ran for 101550 frames Cell Events: 6122 mean_circ: 0.931347
Ran for 102050 frames Cell Events: 6144 mean_circ: 0.931355
Ran for 104050 frames Cell Events: 6259 mean_circ: 0.931265
Ran for 105550 frames Cell Events: 6344 mean_circ: 0.931304
Ran for 107050 frames Cell Events: 6428 mean_circ: 0.931326
Ran for 107550 frames Cell Events: 6452 mean_circ: 0.931381
Ran for 109050 frames Cell Events: 6521 mean_circ: 0.931455
Ran for 110050 frames Cell Events: 6587 mean_circ: 0.931462
Ran for 111050 frames Cell Events: 6649 mean_circ: 0.931533
Ran for 111550 frames Cell Events: 6681 mean_circ: 0.931575
Ran for 112050 frames Cell Events: 6698 mean_circ: 0.931611
Ran for 113050 frames Cell Events: 6776 mean_circ: 0.931554
Ran for 114550 frames Cell Events: 6869 mean_circ: 0.93153
Ran for 115550 frames Cell Events: 6959 mean_circ: 0.931607
Ran for 116050 frames Cell Events: 6989 mean_circ: 0.931545
Ran for 117050 frames Cell Events: 7055 mean_circ: 0.931608
Ran for 118550 frames Cell Events: 7161 mean_circ: 0.931491
Ran for 119050 frames Cell Events: 7178 mean_circ: 0.931478
Ran for 119550 frames Cell Events: 7218 mean_circ: 0.931482
Ran for 120550 frames Cell Events: 7296 mean_circ: 0.931605
Ran for 121050 frames Cell Events: 7323 mean_circ: 0.931561
Ran for 121550 frames Cell Events: 7346 mean_circ: 0.931561
Ran for 122550 frames Cell Events: 7398 mean_circ: 0.931519
Ran for 123050 frames Cell Events: 7418 mean_circ: 0.931494
Ran for 124050 frames Cell Events: 7478 mean_circ: 0.931422
Ran for 125050 frames Cell Events: 7553 mean_circ: 0.931329
Ran for 125550 frames Cell Events: 7576 mean_circ: 0.93131
Ran for 126050 frames Cell Events: 7598 mean_circ: 0.931326
Ran for 126550 frames Cell Events: 7618 mean_circ: 0.931302
Ran for 127050 frames Cell Events: 7636 mean_circ: 0.931294
Ran for 128050 frames Cell Events: 7685 mean_circ: 0.93132
Ran for 129550 frames Cell Events: 7788 mean_circ: 0.93131
Ran for 130550 frames Cell Events: 7845 mean_circ: 0.931394
Ran for 131050 frames Cell Events: 7871 mean_circ: 0.931405
Ran for 132050 frames Cell Events: 7937 mean_circ: 0.931433
Ran for 133050 frames Cell Events: 7982 mean_circ: 0.931382
Ran for 133550 frames Cell Events: 8006 mean_circ: 0.9314
Ran for 134050 frames Cell Events: 8029 mean_circ: 0.931408
Ran for 134550 frames Cell Events: 8069 mean_circ: 0.931408
Ran for 135050 frames Cell Events: 8104 mean_circ: 0.931385



End this measurment..



Stopping time: 7.12448e+008
****************************************
Measurement finished

Mean Circularitiy: 0.931415
Mean Area: 631.685
Mean FL-1 intensity: 3362.09
Mean FL-2 intensity: 4747.82
Mean FL-3 intensity: 0

frames processed: 132242
Total Time: 67697.8ms
Number of Runs (new bg img) 0
Frames collected: 135261
Skipped frames: 24479
Rescued Frames: 860974
Samples DAQ: 33815530
Samples written: 67630348
Samples per Channel: 33815174
FPS: 1998.01

Samples per second: 499502
Expected samples/s: 500000

Events: 8121
Events per second: 119.96
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :210340
# 1 frames :48590
# 2 frames :2443
# 3 frames :879
# 4 frames :483
# 5 frames :205
# 6 frames :1911
# 7 frames :997
# 8 frames :855
# 9 frames :459
# 10 frames :405
# 11 frames :341
# 12 frames :251
# 13 frames :193
# 14 frames :252
# 15 frames :156
# 16 frames :135
# 17 frames :139
# 18 frames :85
# 19 frames :77
# 20 frames :74
# 21 frames :70
# 22 frames :62
# 23 frames :56
# 24 frames :44
# 25 frames :44
# 26 frames :29
# 27 frames :27
# 28 frames :34
# 29 frames :21
# 30 frames :27
# 31 frames :16
# 32 frames :12
# 33 frames :18
# 34 frames :11
# 35 frames :13
# 36 frames :12
# 37 frames :10
# 38 frames :10
# 39 frames :5
# 40 frames :8
# 41 frames :7
# 42 frames :9
# 43 frames :3
# 44 frames :2
# 45 frames :5
# 46 frames :2
# 47 frames :2
# 48 frames :2
# 49 frames :1
# 51 frames :1
# 52 frames :4
# 53 frames :1
# 54 frames :2
# 55 frames :1
# 56 frames :2
# 58 frames :3
# 59 frames :5
# 60 frames :3
# 61 frames :3
# 62 frames :1
# 63 frames :1
# 68 frames :1
# 72 frames :1
# 77 frames :1
# 79 frames :1
# 85 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9973
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0