Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.54.02 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e60898f7c32bd1d5f5b82e82601989f1",
"name": "M1_20170523_1514_dsRNA_F2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:54:23"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8051 (max 8052) in '8051'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.065
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.15897e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 46 mean_circ: 0.95526
Ran for 1550 frames Cell Events: 75 mean_circ: 0.957416
Ran for 2550 frames Cell Events: 131 mean_circ: 0.955022
Ran for 3050 frames Cell Events: 161 mean_circ: 0.954603
Ran for 4050 frames Cell Events: 214 mean_circ: 0.952379
Ran for 5050 frames Cell Events: 275 mean_circ: 0.9509
Ran for 7050 frames Cell Events: 412 mean_circ: 0.95026
Ran for 7550 frames Cell Events: 453 mean_circ: 0.95026
Ran for 9050 frames Cell Events: 554 mean_circ: 0.950399
Ran for 10050 frames Cell Events: 609 mean_circ: 0.950042
Ran for 12050 frames Cell Events: 729 mean_circ: 0.950017
Ran for 13050 frames Cell Events: 790 mean_circ: 0.95054
Ran for 15550 frames Cell Events: 926 mean_circ: 0.950091
Ran for 17050 frames Cell Events: 1037 mean_circ: 0.950329
Ran for 18050 frames Cell Events: 1116 mean_circ: 0.95065
Ran for 18550 frames Cell Events: 1160 mean_circ: 0.951088
Ran for 19550 frames Cell Events: 1212 mean_circ: 0.950977
Ran for 20050 frames Cell Events: 1236 mean_circ: 0.951361
Ran for 21050 frames Cell Events: 1293 mean_circ: 0.951187
Ran for 23550 frames Cell Events: 1448 mean_circ: 0.950642
Ran for 24550 frames Cell Events: 1500 mean_circ: 0.950797
Ran for 25050 frames Cell Events: 1531 mean_circ: 0.950758
Ran for 25550 frames Cell Events: 1562 mean_circ: 0.951004
Ran for 27050 frames Cell Events: 1667 mean_circ: 0.951119
Ran for 28550 frames Cell Events: 1766 mean_circ: 0.951316
Ran for 30050 frames Cell Events: 1866 mean_circ: 0.951195
Ran for 32050 frames Cell Events: 1995 mean_circ: 0.951126
Ran for 32550 frames Cell Events: 2019 mean_circ: 0.951221
Ran for 34050 frames Cell Events: 2118 mean_circ: 0.951029
Ran for 34550 frames Cell Events: 2141 mean_circ: 0.95098
Ran for 35050 frames Cell Events: 2174 mean_circ: 0.950893
Ran for 37050 frames Cell Events: 2293 mean_circ: 0.950847
Ran for 37550 frames Cell Events: 2334 mean_circ: 0.95087
Ran for 39050 frames Cell Events: 2463 mean_circ: 0.950645
Ran for 39550 frames Cell Events: 2487 mean_circ: 0.950774
Ran for 40050 frames Cell Events: 2512 mean_circ: 0.950771
Ran for 41050 frames Cell Events: 2563 mean_circ: 0.95077
Ran for 41550 frames Cell Events: 2597 mean_circ: 0.950796
Ran for 42050 frames Cell Events: 2616 mean_circ: 0.950734
Ran for 44050 frames Cell Events: 2722 mean_circ: 0.950694
Ran for 45550 frames Cell Events: 2804 mean_circ: 0.950736
Ran for 46050 frames Cell Events: 2835 mean_circ: 0.950823
Ran for 46550 frames Cell Events: 2857 mean_circ: 0.95082
Ran for 47550 frames Cell Events: 2898 mean_circ: 0.950732
Ran for 48050 frames Cell Events: 2913 mean_circ: 0.950808
Ran for 49050 frames Cell Events: 2976 mean_circ: 0.950872
Ran for 50050 frames Cell Events: 3031 mean_circ: 0.95044
Ran for 50550 frames Cell Events: 3056 mean_circ: 0.950496
Ran for 51050 frames Cell Events: 3071 mean_circ: 0.95057
Ran for 52050 frames Cell Events: 3137 mean_circ: 0.950645
Ran for 52550 frames Cell Events: 3177 mean_circ: 0.950645
Ran for 53050 frames Cell Events: 3195 mean_circ: 0.950632
Ran for 54050 frames Cell Events: 3259 mean_circ: 0.950678
Ran for 56550 frames Cell Events: 3429 mean_circ: 0.950931
Ran for 58050 frames Cell Events: 3519 mean_circ: 0.950994
Ran for 58550 frames Cell Events: 3547 mean_circ: 0.951024
Ran for 59050 frames Cell Events: 3562 mean_circ: 0.951004
Ran for 59550 frames Cell Events: 3599 mean_circ: 0.951119
Ran for 60050 frames Cell Events: 3631 mean_circ: 0.951029
Ran for 61550 frames Cell Events: 3751 mean_circ: 0.951138
Ran for 65550 frames Cell Events: 4035 mean_circ: 0.951255
Ran for 66550 frames Cell Events: 4090 mean_circ: 0.951122
Ran for 67050 frames Cell Events: 4118 mean_circ: 0.951029
Ran for 68050 frames Cell Events: 4201 mean_circ: 0.951004
Ran for 69050 frames Cell Events: 4254 mean_circ: 0.95101
Ran for 70050 frames Cell Events: 4303 mean_circ: 0.950956
Ran for 70550 frames Cell Events: 4333 mean_circ: 0.950949
Ran for 71050 frames Cell Events: 4351 mean_circ: 0.950923
Ran for 71550 frames Cell Events: 4380 mean_circ: 0.950988
Ran for 72050 frames Cell Events: 4413 mean_circ: 0.950983
Ran for 73050 frames Cell Events: 4486 mean_circ: 0.951072
Ran for 74050 frames Cell Events: 4556 mean_circ: 0.95105
Ran for 74550 frames Cell Events: 4596 mean_circ: 0.951092
Ran for 75550 frames Cell Events: 4646 mean_circ: 0.951037
Ran for 76550 frames Cell Events: 4711 mean_circ: 0.951
Ran for 77050 frames Cell Events: 4750 mean_circ: 0.95098
Ran for 78050 frames Cell Events: 4798 mean_circ: 0.950983
Ran for 79050 frames Cell Events: 4847 mean_circ: 0.950929
Ran for 79550 frames Cell Events: 4866 mean_circ: 0.950931
Ran for 80550 frames Cell Events: 4922 mean_circ: 0.950875
Ran for 81050 frames Cell Events: 4954 mean_circ: 0.950937
Ran for 82050 frames Cell Events: 5029 mean_circ: 0.951109
Ran for 82550 frames Cell Events: 5050 mean_circ: 0.951116
Ran for 83050 frames Cell Events: 5086 mean_circ: 0.951143
Ran for 84050 frames Cell Events: 5128 mean_circ: 0.951114
Ran for 84550 frames Cell Events: 5162 mean_circ: 0.951104
Ran for 86550 frames Cell Events: 5316 mean_circ: 0.950815
Ran for 87050 frames Cell Events: 5327 mean_circ: 0.950865
Ran for 90050 frames Cell Events: 5553 mean_circ: 0.951064
Ran for 90550 frames Cell Events: 5593 mean_circ: 0.951064
Ran for 92050 frames Cell Events: 5691 mean_circ: 0.950898
Ran for 92550 frames Cell Events: 5712 mean_circ: 0.950949
Ran for 95550 frames Cell Events: 5937 mean_circ: 0.950717
Ran for 96050 frames Cell Events: 5958 mean_circ: 0.950734
Ran for 96550 frames Cell Events: 5982 mean_circ: 0.950742
Ran for 97550 frames Cell Events: 6037 mean_circ: 0.950744
Ran for 98550 frames Cell Events: 6107 mean_circ: 0.9507
Ran for 99050 frames Cell Events: 6152 mean_circ: 0.950735
Ran for 100050 frames Cell Events: 6207 mean_circ: 0.950786
Ran for 100550 frames Cell Events: 6241 mean_circ: 0.950803
Ran for 101550 frames Cell Events: 6288 mean_circ: 0.950816
Ran for 102550 frames Cell Events: 6329 mean_circ: 0.950829
Ran for 103050 frames Cell Events: 6360 mean_circ: 0.950846
Ran for 104050 frames Cell Events: 6413 mean_circ: 0.95096
Ran for 106050 frames Cell Events: 6563 mean_circ: 0.951023
Ran for 107550 frames Cell Events: 6639 mean_circ: 0.950954
Ran for 110050 frames Cell Events: 6776 mean_circ: 0.950981
Ran for 112550 frames Cell Events: 6944 mean_circ: 0.951024
Ran for 115050 frames Cell Events: 7106 mean_circ: 0.951035
Ran for 116550 frames Cell Events: 7192 mean_circ: 0.950975
Ran for 118050 frames Cell Events: 7295 mean_circ: 0.950994
Ran for 119050 frames Cell Events: 7350 mean_circ: 0.950994
Ran for 119550 frames Cell Events: 7381 mean_circ: 0.95095
Ran for 120050 frames Cell Events: 7401 mean_circ: 0.950991
Ran for 120550 frames Cell Events: 7423 mean_circ: 0.951005
Ran for 121550 frames Cell Events: 7481 mean_circ: 0.951014
Ran for 122550 frames Cell Events: 7542 mean_circ: 0.951
Ran for 124050 frames Cell Events: 7621 mean_circ: 0.950936
Ran for 125050 frames Cell Events: 7687 mean_circ: 0.950943
Ran for 125550 frames Cell Events: 7717 mean_circ: 0.950994
Ran for 126050 frames Cell Events: 7751 mean_circ: 0.951002
Ran for 127050 frames Cell Events: 7812 mean_circ: 0.951025
Ran for 128550 frames Cell Events: 7916 mean_circ: 0.951062
Ran for 130050 frames Cell Events: 8015 mean_circ: 0.951161
Ran for 130550 frames Cell Events: 8045 mean_circ: 0.951189



End this measurment..



Stopping time: 7.15962e+008
****************************************
Measurement finished

Mean Circularitiy: 0.951177
Mean Area: 566.108
Mean FL-1 intensity: 3579.99
Mean FL-2 intensity: 4618.6
Mean FL-3 intensity: 0

frames processed: 127550
Total Time: 65433.3ms
Number of Runs (new bg img) 0
Frames collected: 130730
Skipped frames: 38887
Rescued Frames: 1632685
Samples DAQ: 32682523
Samples written: 65364962
Samples per Channel: 32682481
FPS: 1997.91

Samples per second: 499477
Expected samples/s: 500000

Events: 8052
Events per second: 123.057
jumped back (PMT): 2881
#Frames read, frequency
# 0 frames :207799
# 1 frames :43203
# 2 frames :2410
# 3 frames :757
# 4 frames :470
# 5 frames :220
# 6 frames :1294
# 7 frames :1028
# 8 frames :870
# 9 frames :491
# 10 frames :393
# 11 frames :258
# 12 frames :260
# 13 frames :171
# 14 frames :260
# 15 frames :211
# 16 frames :168
# 17 frames :117
# 18 frames :101
# 19 frames :75
# 20 frames :71
# 21 frames :94
# 22 frames :88
# 23 frames :79
# 24 frames :49
# 25 frames :38
# 26 frames :35
# 27 frames :38
# 28 frames :19
# 29 frames :30
# 30 frames :21
# 31 frames :18
# 32 frames :18
# 33 frames :17
# 34 frames :18
# 35 frames :20
# 36 frames :12
# 37 frames :13
# 38 frames :11
# 39 frames :5
# 40 frames :8
# 41 frames :5
# 42 frames :19
# 43 frames :10
# 44 frames :6
# 45 frames :7
# 46 frames :8
# 47 frames :7
# 49 frames :2
# 50 frames :4
# 51 frames :4
# 52 frames :3
# 53 frames :4
# 55 frames :6
# 56 frames :1
# 57 frames :2
# 58 frames :2
# 59 frames :1
# 60 frames :1
# 62 frames :2
# 63 frames :2
# 64 frames :1
# 67 frames :2
# 70 frames :1
# 74 frames :1
# 80 frames :1
# 84 frames :2
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.065
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0