Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.54.31 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a229aee1fb5df101186333a0b52e1910",
"name": "M1_20170523_1516_dsRNA_F2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:54:56"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0444
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.16048e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 4050 frames Cell Events: 230 mean_circ: 0.944257
Ran for 5050 frames Cell Events: 294 mean_circ: 0.943119
Ran for 5550 frames Cell Events: 325 mean_circ: 0.943263
Ran for 7050 frames Cell Events: 423 mean_circ: 0.943228
Ran for 8050 frames Cell Events: 479 mean_circ: 0.943506
Ran for 8550 frames Cell Events: 513 mean_circ: 0.942596
Ran for 10050 frames Cell Events: 602 mean_circ: 0.942881
Ran for 11550 frames Cell Events: 700 mean_circ: 0.943321
Ran for 12550 frames Cell Events: 764 mean_circ: 0.943153
Ran for 14050 frames Cell Events: 866 mean_circ: 0.942358
Ran for 15550 frames Cell Events: 962 mean_circ: 0.942458
Ran for 16050 frames Cell Events: 998 mean_circ: 0.942778
Ran for 20550 frames Cell Events: 1337 mean_circ: 0.941486
Ran for 21050 frames Cell Events: 1358 mean_circ: 0.941464
Ran for 22050 frames Cell Events: 1408 mean_circ: 0.94147
Ran for 22550 frames Cell Events: 1440 mean_circ: 0.941503
Ran for 23050 frames Cell Events: 1477 mean_circ: 0.941765
Ran for 24050 frames Cell Events: 1545 mean_circ: 0.941594
Ran for 24550 frames Cell Events: 1578 mean_circ: 0.941645
Ran for 25550 frames Cell Events: 1654 mean_circ: 0.941665
Ran for 26050 frames Cell Events: 1682 mean_circ: 0.941559
Ran for 26550 frames Cell Events: 1709 mean_circ: 0.94156
Ran for 32050 frames Cell Events: 2145 mean_circ: 0.942127
Ran for 33050 frames Cell Events: 2208 mean_circ: 0.942226
Ran for 33550 frames Cell Events: 2232 mean_circ: 0.94241
Ran for 34050 frames Cell Events: 2263 mean_circ: 0.942286
Ran for 34550 frames Cell Events: 2306 mean_circ: 0.942311
Ran for 36050 frames Cell Events: 2401 mean_circ: 0.942167
Ran for 37050 frames Cell Events: 2461 mean_circ: 0.94205
Ran for 37550 frames Cell Events: 2495 mean_circ: 0.942053
Ran for 39550 frames Cell Events: 2635 mean_circ: 0.941962
Ran for 42550 frames Cell Events: 2804 mean_circ: 0.942065
Ran for 43050 frames Cell Events: 2838 mean_circ: 0.941966
Ran for 43550 frames Cell Events: 2878 mean_circ: 0.942034
Ran for 44050 frames Cell Events: 2912 mean_circ: 0.941997
Ran for 44550 frames Cell Events: 2940 mean_circ: 0.941888
Ran for 45550 frames Cell Events: 3007 mean_circ: 0.941897
Ran for 46050 frames Cell Events: 3042 mean_circ: 0.94178
Ran for 47550 frames Cell Events: 3132 mean_circ: 0.941803
Ran for 48550 frames Cell Events: 3194 mean_circ: 0.941792
Ran for 49050 frames Cell Events: 3229 mean_circ: 0.941794
Ran for 50550 frames Cell Events: 3339 mean_circ: 0.941666
Ran for 51050 frames Cell Events: 3363 mean_circ: 0.941696
Ran for 52050 frames Cell Events: 3387 mean_circ: 0.941608
Ran for 53550 frames Cell Events: 3507 mean_circ: 0.941605
Ran for 54050 frames Cell Events: 3540 mean_circ: 0.941708
Ran for 55050 frames Cell Events: 3609 mean_circ: 0.941722
Ran for 56050 frames Cell Events: 3668 mean_circ: 0.941683
Ran for 56550 frames Cell Events: 3700 mean_circ: 0.941732
Ran for 57050 frames Cell Events: 3726 mean_circ: 0.941723
Ran for 58550 frames Cell Events: 3807 mean_circ: 0.941702
Ran for 59050 frames Cell Events: 3842 mean_circ: 0.941698
Ran for 60550 frames Cell Events: 3949 mean_circ: 0.941449
Ran for 62550 frames Cell Events: 4114 mean_circ: 0.941293
Ran for 63050 frames Cell Events: 4142 mean_circ: 0.941319
Ran for 64050 frames Cell Events: 4208 mean_circ: 0.941371
Ran for 64550 frames Cell Events: 4237 mean_circ: 0.941394
Ran for 65050 frames Cell Events: 4265 mean_circ: 0.941366
Ran for 66050 frames Cell Events: 4319 mean_circ: 0.941284
Ran for 66550 frames Cell Events: 4354 mean_circ: 0.941215
Ran for 69050 frames Cell Events: 4522 mean_circ: 0.941219
Ran for 69550 frames Cell Events: 4550 mean_circ: 0.941306
Ran for 70050 frames Cell Events: 4583 mean_circ: 0.941297
Ran for 70550 frames Cell Events: 4613 mean_circ: 0.941242
Ran for 72050 frames Cell Events: 4736 mean_circ: 0.941244
Ran for 73050 frames Cell Events: 4797 mean_circ: 0.94126
Ran for 73550 frames Cell Events: 4822 mean_circ: 0.94127
Ran for 74050 frames Cell Events: 4850 mean_circ: 0.941251
Ran for 74550 frames Cell Events: 4887 mean_circ: 0.941269
Ran for 75050 frames Cell Events: 4925 mean_circ: 0.941216
Ran for 76550 frames Cell Events: 5043 mean_circ: 0.941265
Ran for 78050 frames Cell Events: 5167 mean_circ: 0.941224
Ran for 79050 frames Cell Events: 5216 mean_circ: 0.941298
Ran for 79550 frames Cell Events: 5253 mean_circ: 0.9413
Ran for 80550 frames Cell Events: 5330 mean_circ: 0.941295
Ran for 81550 frames Cell Events: 5400 mean_circ: 0.941278
Ran for 82550 frames Cell Events: 5463 mean_circ: 0.941278
Ran for 83550 frames Cell Events: 5531 mean_circ: 0.941254
Ran for 85550 frames Cell Events: 5687 mean_circ: 0.941207
Ran for 87050 frames Cell Events: 5793 mean_circ: 0.941211
Ran for 87550 frames Cell Events: 5825 mean_circ: 0.941125
Ran for 88050 frames Cell Events: 5870 mean_circ: 0.941122
Ran for 88550 frames Cell Events: 5897 mean_circ: 0.941134
Ran for 89050 frames Cell Events: 5926 mean_circ: 0.941204
Ran for 90050 frames Cell Events: 5992 mean_circ: 0.941028
Ran for 91050 frames Cell Events: 6069 mean_circ: 0.940908
Ran for 91550 frames Cell Events: 6104 mean_circ: 0.940935
Ran for 92550 frames Cell Events: 6165 mean_circ: 0.940983
Ran for 95050 frames Cell Events: 6337 mean_circ: 0.940936
Ran for 96050 frames Cell Events: 6408 mean_circ: 0.940837
Ran for 97550 frames Cell Events: 6521 mean_circ: 0.940736
Ran for 99550 frames Cell Events: 6633 mean_circ: 0.940787
Ran for 100050 frames Cell Events: 6671 mean_circ: 0.940819
Ran for 101550 frames Cell Events: 6798 mean_circ: 0.940955
Ran for 102050 frames Cell Events: 6826 mean_circ: 0.940889
Ran for 102550 frames Cell Events: 6868 mean_circ: 0.94093
Ran for 105550 frames Cell Events: 7116 mean_circ: 0.940989
Ran for 108050 frames Cell Events: 7314 mean_circ: 0.941107
Ran for 111050 frames Cell Events: 7534 mean_circ: 0.941214
Ran for 111550 frames Cell Events: 7565 mean_circ: 0.941242
Ran for 112050 frames Cell Events: 7610 mean_circ: 0.941253
Ran for 112550 frames Cell Events: 7639 mean_circ: 0.941256
Ran for 113050 frames Cell Events: 7666 mean_circ: 0.94129
Ran for 114050 frames Cell Events: 7729 mean_circ: 0.94124
Ran for 114550 frames Cell Events: 7757 mean_circ: 0.941272
Ran for 115050 frames Cell Events: 7783 mean_circ: 0.941312
Ran for 115550 frames Cell Events: 7809 mean_circ: 0.941312
Ran for 117050 frames Cell Events: 7930 mean_circ: 0.941312
Ran for 117550 frames Cell Events: 7955 mean_circ: 0.941365
Ran for 118550 frames Cell Events: 8016 mean_circ: 0.941371
Ran for 120550 frames Cell Events: 8159 mean_circ: 0.941305
Ran for 121050 frames Cell Events: 8187 mean_circ: 0.941326
Ran for 121550 frames Cell Events: 8217 mean_circ: 0.941311
Ran for 123050 frames Cell Events: 8325 mean_circ: 0.941322
Ran for 125050 frames Cell Events: 8489 mean_circ: 0.941309
Ran for 126050 frames Cell Events: 8558 mean_circ: 0.941248
Ran for 128550 frames Cell Events: 8766 mean_circ: 0.941283
Ran for 130050 frames Cell Events: 8857 mean_circ: 0.941261
Ran for 131550 frames Cell Events: 8952 mean_circ: 0.941138
Ran for 132550 frames Cell Events: 9025 mean_circ: 0.941138
Ran for 133050 frames Cell Events: 9046 mean_circ: 0.941146
Ran for 133550 frames Cell Events: 9078 mean_circ: 0.941166
Ran for 134050 frames Cell Events: 9106 mean_circ: 0.941162
Ran for 134550 frames Cell Events: 9123 mean_circ: 0.941165
Ran for 136550 frames Cell Events: 9250 mean_circ: 0.94115
Ran for 137050 frames Cell Events: 9286 mean_circ: 0.941187
Ran for 140050 frames Cell Events: 9507 mean_circ: 0.941133
Ran for 140550 frames Cell Events: 9552 mean_circ: 0.9411
Ran for 141050 frames Cell Events: 9591 mean_circ: 0.941092
Ran for 142050 frames Cell Events: 9670 mean_circ: 0.94108
Ran for 142550 frames Cell Events: 9722 mean_circ: 0.941049
Ran for 143050 frames Cell Events: 9758 mean_circ: 0.941045
Ran for 143550 frames Cell Events: 9791 mean_circ: 0.941088
Ran for 145050 frames Cell Events: 9902 mean_circ: 0.941058



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 7.16122e+008
****************************************
Measurement finished

Mean Circularitiy: 0.941057
Mean Area: 560.587
Mean FL-1 intensity: 3324.31
Mean FL-2 intensity: 4850.17
Mean FL-3 intensity: 0

frames processed: 143539
Total Time: 73235.9ms
Number of Runs (new bg img) 0
Frames collected: 146306
Skipped frames: 41654
Rescued Frames: 1714327
Samples DAQ: 36577739
Samples written: 73153102
Samples per Channel: 36576551
FPS: 1997.74

Samples per second: 499435
Expected samples/s: 500000

Events: 10001
Events per second: 136.559
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :230643
# 1 frames :49535
# 2 frames :2545
# 3 frames :934
# 4 frames :444
# 5 frames :204
# 6 frames :2645
# 7 frames :1300
# 8 frames :977
# 9 frames :561
# 10 frames :370
# 11 frames :285
# 12 frames :213
# 13 frames :241
# 14 frames :286
# 15 frames :234
# 16 frames :154
# 17 frames :138
# 18 frames :92
# 19 frames :98
# 20 frames :82
# 21 frames :81
# 22 frames :63
# 23 frames :54
# 24 frames :53
# 25 frames :30
# 26 frames :35
# 27 frames :29
# 28 frames :20
# 29 frames :23
# 30 frames :22
# 31 frames :22
# 32 frames :16
# 33 frames :14
# 34 frames :7
# 35 frames :12
# 36 frames :9
# 37 frames :7
# 38 frames :6
# 39 frames :6
# 40 frames :7
# 41 frames :8
# 42 frames :7
# 43 frames :9
# 44 frames :1
# 45 frames :2
# 46 frames :4
# 47 frames :5
# 48 frames :2
# 49 frames :3
# 50 frames :2
# 51 frames :2
# 52 frames :1
# 53 frames :5
# 54 frames :4
# 55 frames :1
# 57 frames :1
# 59 frames :2
# 60 frames :1
# 61 frames :1
# 64 frames :1
# 66 frames :1
# 75 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0444
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0