Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.55.05 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2ca4c74783d2c632bfb4b1da0063fd42",
"name": "M1_20170523_1526_dsRNA_F6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:55:30"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9612 (max 9613) in '9612'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1156
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.16612e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 30 mean_circ: 0.94239
Ran for 2050 frames Cell Events: 104 mean_circ: 0.951198
Ran for 2550 frames Cell Events: 131 mean_circ: 0.953636
Ran for 3050 frames Cell Events: 169 mean_circ: 0.953636
Ran for 3550 frames Cell Events: 196 mean_circ: 0.954296
Ran for 4550 frames Cell Events: 248 mean_circ: 0.953433
Ran for 5050 frames Cell Events: 277 mean_circ: 0.952942
Ran for 5550 frames Cell Events: 299 mean_circ: 0.952095
Ran for 7550 frames Cell Events: 421 mean_circ: 0.950634
Ran for 8050 frames Cell Events: 444 mean_circ: 0.950848
Ran for 9050 frames Cell Events: 496 mean_circ: 0.950795
Ran for 9550 frames Cell Events: 517 mean_circ: 0.950657
Ran for 10050 frames Cell Events: 538 mean_circ: 0.950232
Ran for 12050 frames Cell Events: 650 mean_circ: 0.951251
Ran for 12550 frames Cell Events: 672 mean_circ: 0.951668
Ran for 14050 frames Cell Events: 751 mean_circ: 0.951363
Ran for 15550 frames Cell Events: 849 mean_circ: 0.951171
Ran for 16050 frames Cell Events: 872 mean_circ: 0.950781
Ran for 18550 frames Cell Events: 1007 mean_circ: 0.950354
Ran for 19050 frames Cell Events: 1027 mean_circ: 0.949937
Ran for 20550 frames Cell Events: 1127 mean_circ: 0.950434
Ran for 21550 frames Cell Events: 1215 mean_circ: 0.950303
Ran for 22050 frames Cell Events: 1234 mean_circ: 0.950496
Ran for 24050 frames Cell Events: 1357 mean_circ: 0.950575
Ran for 25050 frames Cell Events: 1418 mean_circ: 0.950338
Ran for 25550 frames Cell Events: 1440 mean_circ: 0.950374
Ran for 26050 frames Cell Events: 1465 mean_circ: 0.950432
Ran for 27550 frames Cell Events: 1553 mean_circ: 0.950368
Ran for 28050 frames Cell Events: 1595 mean_circ: 0.950368
Ran for 28550 frames Cell Events: 1623 mean_circ: 0.950155
Ran for 29050 frames Cell Events: 1646 mean_circ: 0.950221
Ran for 30550 frames Cell Events: 1742 mean_circ: 0.950366
Ran for 31550 frames Cell Events: 1797 mean_circ: 0.950239
Ran for 33050 frames Cell Events: 1878 mean_circ: 0.950352
Ran for 33550 frames Cell Events: 1904 mean_circ: 0.950454
Ran for 34550 frames Cell Events: 1962 mean_circ: 0.950305
Ran for 37050 frames Cell Events: 2089 mean_circ: 0.950069
Ran for 38050 frames Cell Events: 2143 mean_circ: 0.950129
Ran for 39550 frames Cell Events: 2252 mean_circ: 0.950061
Ran for 43050 frames Cell Events: 2449 mean_circ: 0.94962
Ran for 44550 frames Cell Events: 2525 mean_circ: 0.949696
Ran for 47050 frames Cell Events: 2664 mean_circ: 0.949695
Ran for 47550 frames Cell Events: 2685 mean_circ: 0.949751
Ran for 48050 frames Cell Events: 2706 mean_circ: 0.949793
Ran for 48550 frames Cell Events: 2729 mean_circ: 0.949817
Ran for 49050 frames Cell Events: 2758 mean_circ: 0.949818
Ran for 50550 frames Cell Events: 2839 mean_circ: 0.949852
Ran for 51050 frames Cell Events: 2867 mean_circ: 0.949942
Ran for 51550 frames Cell Events: 2899 mean_circ: 0.949991
Ran for 53050 frames Cell Events: 2967 mean_circ: 0.950158
Ran for 53550 frames Cell Events: 2987 mean_circ: 0.950007
Ran for 54050 frames Cell Events: 3008 mean_circ: 0.950052
Ran for 55050 frames Cell Events: 3075 mean_circ: 0.950029
Ran for 55550 frames Cell Events: 3099 mean_circ: 0.950097
Ran for 56050 frames Cell Events: 3119 mean_circ: 0.950105
Ran for 57550 frames Cell Events: 3198 mean_circ: 0.94999
Ran for 58050 frames Cell Events: 3216 mean_circ: 0.949989
Ran for 58550 frames Cell Events: 3249 mean_circ: 0.949833
Ran for 61050 frames Cell Events: 3385 mean_circ: 0.949819
Ran for 62050 frames Cell Events: 3426 mean_circ: 0.949876
Ran for 63050 frames Cell Events: 3481 mean_circ: 0.94974
Ran for 65050 frames Cell Events: 3626 mean_circ: 0.949751
Ran for 65550 frames Cell Events: 3649 mean_circ: 0.949703
Ran for 66550 frames Cell Events: 3708 mean_circ: 0.949761
Ran for 67050 frames Cell Events: 3724 mean_circ: 0.949755
Ran for 68050 frames Cell Events: 3803 mean_circ: 0.94985
Ran for 68550 frames Cell Events: 3830 mean_circ: 0.949869
Ran for 69050 frames Cell Events: 3857 mean_circ: 0.949847
Ran for 70050 frames Cell Events: 3904 mean_circ: 0.949878
Ran for 71050 frames Cell Events: 3945 mean_circ: 0.949905
Ran for 71550 frames Cell Events: 3966 mean_circ: 0.949946
Ran for 72050 frames Cell Events: 3995 mean_circ: 0.949948
Ran for 73050 frames Cell Events: 4063 mean_circ: 0.949973
Ran for 73550 frames Cell Events: 4092 mean_circ: 0.949954
Ran for 74550 frames Cell Events: 4162 mean_circ: 0.950027
Ran for 75550 frames Cell Events: 4223 mean_circ: 0.949938
Ran for 76050 frames Cell Events: 4253 mean_circ: 0.949991
Ran for 76550 frames Cell Events: 4278 mean_circ: 0.949944
Ran for 77050 frames Cell Events: 4319 mean_circ: 0.949941
Ran for 77550 frames Cell Events: 4357 mean_circ: 0.949944
Ran for 78050 frames Cell Events: 4383 mean_circ: 0.949984
Ran for 79050 frames Cell Events: 4450 mean_circ: 0.949949
Ran for 80550 frames Cell Events: 4551 mean_circ: 0.949924
Ran for 81050 frames Cell Events: 4580 mean_circ: 0.949926
Ran for 82550 frames Cell Events: 4680 mean_circ: 0.94992
Ran for 84550 frames Cell Events: 4803 mean_circ: 0.949935
Ran for 85050 frames Cell Events: 4831 mean_circ: 0.949903
Ran for 86550 frames Cell Events: 4911 mean_circ: 0.949805
Ran for 87050 frames Cell Events: 4936 mean_circ: 0.949754
Ran for 88050 frames Cell Events: 5009 mean_circ: 0.949723
Ran for 88550 frames Cell Events: 5044 mean_circ: 0.949687
Ran for 89550 frames Cell Events: 5097 mean_circ: 0.949674
Ran for 90050 frames Cell Events: 5118 mean_circ: 0.949681
Ran for 93550 frames Cell Events: 5345 mean_circ: 0.949606
Ran for 94050 frames Cell Events: 5378 mean_circ: 0.94956
Ran for 94550 frames Cell Events: 5408 mean_circ: 0.949557
Ran for 95050 frames Cell Events: 5447 mean_circ: 0.949566
Ran for 97550 frames Cell Events: 5625 mean_circ: 0.949579
Ran for 99550 frames Cell Events: 5744 mean_circ: 0.949688
Ran for 100050 frames Cell Events: 5771 mean_circ: 0.94967
Ran for 102050 frames Cell Events: 5904 mean_circ: 0.949665
Ran for 103550 frames Cell Events: 6001 mean_circ: 0.949808
Ran for 104050 frames Cell Events: 6027 mean_circ: 0.949806
Ran for 104550 frames Cell Events: 6048 mean_circ: 0.949801
Ran for 107050 frames Cell Events: 6236 mean_circ: 0.949693
Ran for 107550 frames Cell Events: 6264 mean_circ: 0.949703
Ran for 108050 frames Cell Events: 6285 mean_circ: 0.949707
Ran for 111050 frames Cell Events: 6509 mean_circ: 0.94963
Ran for 111550 frames Cell Events: 6534 mean_circ: 0.949641
Ran for 113050 frames Cell Events: 6623 mean_circ: 0.949654
Ran for 113550 frames Cell Events: 6655 mean_circ: 0.949596
Ran for 114050 frames Cell Events: 6696 mean_circ: 0.949489
Ran for 114550 frames Cell Events: 6718 mean_circ: 0.949542
Ran for 115050 frames Cell Events: 6752 mean_circ: 0.94957
Ran for 116050 frames Cell Events: 6817 mean_circ: 0.949587
Ran for 117050 frames Cell Events: 6889 mean_circ: 0.94963
Ran for 117550 frames Cell Events: 6932 mean_circ: 0.949553
Ran for 118050 frames Cell Events: 6962 mean_circ: 0.949593
Ran for 118550 frames Cell Events: 7001 mean_circ: 0.949597
Ran for 119050 frames Cell Events: 7051 mean_circ: 0.949597
Ran for 121050 frames Cell Events: 7179 mean_circ: 0.949526
Ran for 121550 frames Cell Events: 7206 mean_circ: 0.949553
Ran for 122550 frames Cell Events: 7268 mean_circ: 0.94946
Ran for 123050 frames Cell Events: 7299 mean_circ: 0.94946
Ran for 124050 frames Cell Events: 7355 mean_circ: 0.949527
Ran for 125050 frames Cell Events: 7404 mean_circ: 0.949405
Ran for 125550 frames Cell Events: 7435 mean_circ: 0.949414
Ran for 126050 frames Cell Events: 7470 mean_circ: 0.949376
Ran for 126550 frames Cell Events: 7513 mean_circ: 0.949383
Ran for 127550 frames Cell Events: 7579 mean_circ: 0.949418
Ran for 128550 frames Cell Events: 7658 mean_circ: 0.949458
Ran for 129550 frames Cell Events: 7710 mean_circ: 0.949496
Ran for 130050 frames Cell Events: 7744 mean_circ: 0.94953
Ran for 130550 frames Cell Events: 7781 mean_circ: 0.94953
Ran for 133050 frames Cell Events: 7958 mean_circ: 0.949551
Ran for 133550 frames Cell Events: 7994 mean_circ: 0.949535
Ran for 134050 frames Cell Events: 8029 mean_circ: 0.949594
Ran for 135050 frames Cell Events: 8079 mean_circ: 0.949577
Ran for 135550 frames Cell Events: 8118 mean_circ: 0.94957
Ran for 136550 frames Cell Events: 8195 mean_circ: 0.94964
Ran for 137050 frames Cell Events: 8224 mean_circ: 0.949636
Ran for 137550 frames Cell Events: 8246 mean_circ: 0.949634
Ran for 138550 frames Cell Events: 8314 mean_circ: 0.949644
Ran for 140050 frames Cell Events: 8414 mean_circ: 0.94957
Ran for 142050 frames Cell Events: 8531 mean_circ: 0.949578
Ran for 142550 frames Cell Events: 8561 mean_circ: 0.949591
Ran for 143550 frames Cell Events: 8640 mean_circ: 0.949598
Ran for 144050 frames Cell Events: 8673 mean_circ: 0.949596
Ran for 145050 frames Cell Events: 8757 mean_circ: 0.949584
Ran for 147050 frames Cell Events: 8921 mean_circ: 0.949549
Ran for 147550 frames Cell Events: 8958 mean_circ: 0.94954
Ran for 148050 frames Cell Events: 8989 mean_circ: 0.949543
Ran for 151050 frames Cell Events: 9173 mean_circ: 0.949401
Ran for 152550 frames Cell Events: 9261 mean_circ: 0.949439
Ran for 155050 frames Cell Events: 9398 mean_circ: 0.949469
Ran for 155550 frames Cell Events: 9425 mean_circ: 0.949472
Ran for 156550 frames Cell Events: 9505 mean_circ: 0.949493



End this measurment..



Stopping time: 7.16691e+008
****************************************
Measurement finished

Mean Circularitiy: 0.949505
Mean Area: 585.053
Mean FL-1 intensity: 3529.85
Mean FL-2 intensity: 3995.49
Mean FL-3 intensity: 0

frames processed: 153279
Total Time: 78973.6ms
Number of Runs (new bg img) 0
Frames collected: 157828
Skipped frames: 46203
Rescued Frames: 1801792
Samples DAQ: 39457043
Samples written: 78914000
Samples per Channel: 39457000
FPS: 1998.49

Samples per second: 499623
Expected samples/s: 500000

Events: 9613
Events per second: 121.724
jumped back (PMT): 3488
#Frames read, frequency
# 0 frames :247658
# 1 frames :53220
# 2 frames :2783
# 3 frames :1044
# 4 frames :531
# 5 frames :273
# 6 frames :1826
# 7 frames :1328
# 8 frames :1045
# 9 frames :573
# 10 frames :429
# 11 frames :347
# 12 frames :287
# 13 frames :232
# 14 frames :321
# 15 frames :240
# 16 frames :152
# 17 frames :152
# 18 frames :110
# 19 frames :86
# 20 frames :108
# 21 frames :79
# 22 frames :84
# 23 frames :62
# 24 frames :51
# 25 frames :48
# 26 frames :47
# 27 frames :39
# 28 frames :39
# 29 frames :31
# 30 frames :25
# 31 frames :26
# 32 frames :17
# 33 frames :26
# 34 frames :23
# 35 frames :15
# 36 frames :11
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# 38 frames :6
# 39 frames :7
# 40 frames :10
# 41 frames :13
# 42 frames :6
# 43 frames :12
# 44 frames :6
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# 48 frames :3
# 49 frames :4
# 50 frames :1
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# 52 frames :1
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# 55 frames :3
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# 68 frames :1
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# 70 frames :3
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# 72 frames :1
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# 89 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1156
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0