Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.55.37 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "6f59814b84edd849c65bcc5276109464",
"name": "M1_20170523_1529_dsRNA_F6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:55:58"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8347 (max 8348) in '8347'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.118
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.16782e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 57 mean_circ: 0.928523
Ran for 1550 frames Cell Events: 93 mean_circ: 0.930323
Ran for 2050 frames Cell Events: 125 mean_circ: 0.934984
Ran for 3550 frames Cell Events: 184 mean_circ: 0.938353
Ran for 4550 frames Cell Events: 262 mean_circ: 0.937164
Ran for 5050 frames Cell Events: 289 mean_circ: 0.937476
Ran for 6550 frames Cell Events: 384 mean_circ: 0.938184
Ran for 7050 frames Cell Events: 415 mean_circ: 0.9375
Ran for 7550 frames Cell Events: 441 mean_circ: 0.937664
Ran for 8050 frames Cell Events: 479 mean_circ: 0.938082
Ran for 9050 frames Cell Events: 558 mean_circ: 0.938496
Ran for 9550 frames Cell Events: 593 mean_circ: 0.938685
Ran for 13550 frames Cell Events: 868 mean_circ: 0.938856
Ran for 15050 frames Cell Events: 965 mean_circ: 0.938529
Ran for 16550 frames Cell Events: 1051 mean_circ: 0.938323
Ran for 19050 frames Cell Events: 1205 mean_circ: 0.939166
Ran for 19550 frames Cell Events: 1239 mean_circ: 0.939266
Ran for 20050 frames Cell Events: 1263 mean_circ: 0.939399
Ran for 20550 frames Cell Events: 1287 mean_circ: 0.939287
Ran for 21550 frames Cell Events: 1351 mean_circ: 0.939426
Ran for 22050 frames Cell Events: 1378 mean_circ: 0.939389
Ran for 22550 frames Cell Events: 1411 mean_circ: 0.939377
Ran for 23050 frames Cell Events: 1440 mean_circ: 0.9393
Ran for 23550 frames Cell Events: 1473 mean_circ: 0.939435
Ran for 24050 frames Cell Events: 1499 mean_circ: 0.93932
Ran for 24550 frames Cell Events: 1526 mean_circ: 0.939433
Ran for 26050 frames Cell Events: 1618 mean_circ: 0.93882
Ran for 27050 frames Cell Events: 1695 mean_circ: 0.938952
Ran for 27550 frames Cell Events: 1733 mean_circ: 0.939096
Ran for 28050 frames Cell Events: 1781 mean_circ: 0.939051
Ran for 28550 frames Cell Events: 1808 mean_circ: 0.938726
Ran for 29050 frames Cell Events: 1838 mean_circ: 0.938702
Ran for 31550 frames Cell Events: 1999 mean_circ: 0.939024
Ran for 32050 frames Cell Events: 2031 mean_circ: 0.939018
Ran for 32550 frames Cell Events: 2058 mean_circ: 0.939062
Ran for 35550 frames Cell Events: 2266 mean_circ: 0.938791
Ran for 36550 frames Cell Events: 2336 mean_circ: 0.938666
Ran for 37050 frames Cell Events: 2366 mean_circ: 0.938642
Ran for 39050 frames Cell Events: 2472 mean_circ: 0.938773
Ran for 39550 frames Cell Events: 2519 mean_circ: 0.938773
Ran for 41050 frames Cell Events: 2640 mean_circ: 0.938685
Ran for 41550 frames Cell Events: 2689 mean_circ: 0.938796
Ran for 42050 frames Cell Events: 2720 mean_circ: 0.938789
Ran for 44050 frames Cell Events: 2881 mean_circ: 0.938582
Ran for 44550 frames Cell Events: 2914 mean_circ: 0.938806
Ran for 46050 frames Cell Events: 3020 mean_circ: 0.938801
Ran for 46550 frames Cell Events: 3052 mean_circ: 0.93886
Ran for 47050 frames Cell Events: 3088 mean_circ: 0.938755
Ran for 48550 frames Cell Events: 3189 mean_circ: 0.938715
Ran for 49050 frames Cell Events: 3215 mean_circ: 0.938704
Ran for 52550 frames Cell Events: 3453 mean_circ: 0.938472
Ran for 54550 frames Cell Events: 3627 mean_circ: 0.938718
Ran for 55550 frames Cell Events: 3713 mean_circ: 0.938749
Ran for 57050 frames Cell Events: 3823 mean_circ: 0.938792
Ran for 57550 frames Cell Events: 3848 mean_circ: 0.938824
Ran for 58050 frames Cell Events: 3882 mean_circ: 0.938772
Ran for 59050 frames Cell Events: 3942 mean_circ: 0.938731
Ran for 59550 frames Cell Events: 3974 mean_circ: 0.938723
Ran for 62550 frames Cell Events: 4184 mean_circ: 0.938762
Ran for 64550 frames Cell Events: 4275 mean_circ: 0.938956
Ran for 65050 frames Cell Events: 4315 mean_circ: 0.938919
Ran for 66550 frames Cell Events: 4429 mean_circ: 0.938861
Ran for 69550 frames Cell Events: 4663 mean_circ: 0.938948
Ran for 70050 frames Cell Events: 4696 mean_circ: 0.938891
Ran for 72050 frames Cell Events: 4850 mean_circ: 0.938998
Ran for 73050 frames Cell Events: 4928 mean_circ: 0.939015
Ran for 73550 frames Cell Events: 4952 mean_circ: 0.939031
Ran for 74550 frames Cell Events: 5024 mean_circ: 0.939047
Ran for 76050 frames Cell Events: 5143 mean_circ: 0.93927
Ran for 76550 frames Cell Events: 5176 mean_circ: 0.939247
Ran for 77550 frames Cell Events: 5252 mean_circ: 0.939239
Ran for 78050 frames Cell Events: 5278 mean_circ: 0.939226
Ran for 78550 frames Cell Events: 5298 mean_circ: 0.939241
Ran for 79050 frames Cell Events: 5319 mean_circ: 0.939217
Ran for 79550 frames Cell Events: 5358 mean_circ: 0.939218
Ran for 81550 frames Cell Events: 5521 mean_circ: 0.939276
Ran for 82050 frames Cell Events: 5543 mean_circ: 0.939268
Ran for 82550 frames Cell Events: 5582 mean_circ: 0.939281
Ran for 83050 frames Cell Events: 5613 mean_circ: 0.939271
Ran for 83550 frames Cell Events: 5639 mean_circ: 0.939292
Ran for 84050 frames Cell Events: 5662 mean_circ: 0.939229
Ran for 84550 frames Cell Events: 5691 mean_circ: 0.939163
Ran for 85050 frames Cell Events: 5731 mean_circ: 0.939237
Ran for 86050 frames Cell Events: 5796 mean_circ: 0.939239
Ran for 86550 frames Cell Events: 5833 mean_circ: 0.939234
Ran for 88050 frames Cell Events: 5896 mean_circ: 0.939235
Ran for 89050 frames Cell Events: 5961 mean_circ: 0.939307
Ran for 90050 frames Cell Events: 6021 mean_circ: 0.939312
Ran for 90550 frames Cell Events: 6061 mean_circ: 0.939312
Ran for 91550 frames Cell Events: 6143 mean_circ: 0.939259
Ran for 93550 frames Cell Events: 6233 mean_circ: 0.939195
Ran for 94050 frames Cell Events: 6266 mean_circ: 0.939208
Ran for 96050 frames Cell Events: 6393 mean_circ: 0.939162
Ran for 98050 frames Cell Events: 6531 mean_circ: 0.939119
Ran for 98550 frames Cell Events: 6558 mean_circ: 0.939146
Ran for 99050 frames Cell Events: 6582 mean_circ: 0.939164
Ran for 101050 frames Cell Events: 6696 mean_circ: 0.939228
Ran for 101550 frames Cell Events: 6718 mean_circ: 0.939215
Ran for 102050 frames Cell Events: 6750 mean_circ: 0.939199
Ran for 104050 frames Cell Events: 6850 mean_circ: 0.939196
Ran for 104550 frames Cell Events: 6877 mean_circ: 0.939182
Ran for 105550 frames Cell Events: 6940 mean_circ: 0.939274
Ran for 108050 frames Cell Events: 7114 mean_circ: 0.939205
Ran for 109050 frames Cell Events: 7174 mean_circ: 0.939261
Ran for 109550 frames Cell Events: 7210 mean_circ: 0.939253
Ran for 110050 frames Cell Events: 7239 mean_circ: 0.939134
Ran for 110550 frames Cell Events: 7275 mean_circ: 0.939113
Ran for 111550 frames Cell Events: 7333 mean_circ: 0.939121
Ran for 112050 frames Cell Events: 7376 mean_circ: 0.939102
Ran for 114050 frames Cell Events: 7470 mean_circ: 0.93919
Ran for 114550 frames Cell Events: 7527 mean_circ: 0.93919
Ran for 115550 frames Cell Events: 7608 mean_circ: 0.938979
Ran for 116050 frames Cell Events: 7656 mean_circ: 0.938892
Ran for 117050 frames Cell Events: 7741 mean_circ: 0.93888
Ran for 119550 frames Cell Events: 7942 mean_circ: 0.938858
Ran for 120050 frames Cell Events: 7988 mean_circ: 0.93873
Ran for 120550 frames Cell Events: 8029 mean_circ: 0.938716
Ran for 123550 frames Cell Events: 8265 mean_circ: 0.938588
Ran for 124050 frames Cell Events: 8291 mean_circ: 0.938597



End this measurment..



Stopping time: 7.16845e+008
****************************************
Measurement finished

Mean Circularitiy: 0.93868
Mean Area: 585.383
Mean FL-1 intensity: 3264.67
Mean FL-2 intensity: 4173.63
Mean FL-3 intensity: 0

frames processed: 120287
Total Time: 62531.9ms
Number of Runs (new bg img) 0
Frames collected: 124922
Skipped frames: 50838
Rescued Frames: 1869633
Samples DAQ: 31230507
Samples written: 62460780
Samples per Channel: 31230390
FPS: 1997.73

Samples per second: 499431
Expected samples/s: 500000

Events: 8348
Events per second: 133.5
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :197355
# 1 frames :42064
# 2 frames :2096
# 3 frames :779
# 4 frames :370
# 5 frames :159
# 6 frames :2358
# 7 frames :939
# 8 frames :895
# 9 frames :515
# 10 frames :312
# 11 frames :214
# 12 frames :177
# 13 frames :206
# 14 frames :255
# 15 frames :186
# 16 frames :165
# 17 frames :104
# 18 frames :74
# 19 frames :67
# 20 frames :66
# 21 frames :65
# 22 frames :54
# 23 frames :56
# 24 frames :40
# 25 frames :31
# 26 frames :27
# 27 frames :24
# 28 frames :13
# 29 frames :18
# 30 frames :17
# 31 frames :8
# 32 frames :16
# 33 frames :13
# 34 frames :10
# 35 frames :10
# 36 frames :7
# 37 frames :7
# 38 frames :2
# 39 frames :6
# 40 frames :4
# 41 frames :2
# 43 frames :4
# 44 frames :6
# 45 frames :2
# 46 frames :1
# 48 frames :1
# 50 frames :1
# 53 frames :1
# 54 frames :1
# 55 frames :2
# 60 frames :2
# 61 frames :2
# 62 frames :1
# 63 frames :1
# 64 frames :1
# 66 frames :1
# 68 frames :1
# 71 frames :1
# 75 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.118
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0